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All Journal Jurnal Veteriner Jurnal VOK@SINDO The Journal of Pure and Applied Chemistry Research Formosa Journal of Multidisciplinary Research (FJMR)

Chanif Mahdi

Unknown Affiliation

Author-ID : 440491

Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Education Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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Articles

Formalin Borax and Rhodamin Quick Detection Instruments Discovered by Researcher and Education Staff of Brawijaya University Produced By Biochem Laboratory Chanif Mahdi
Jurnal VOK@SINDO Vol 1, No 1 (2013)
Publisher : Program Pendidikan Vokasi Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.453 KB)

Formalin borax and rhodamin are toxic and carcinogenic substances which could cause cells damage in short term, and could cause 50 kond of degenerative disease and cancer in long term. The aims of this study are to know the potent and effectiveness of three of instruments of reagent kit testers: Formalin main reagent (FMR), Borax main reagent (BMR) and Colour main reagent (CMR) to detect formalin, borax and rhodamin on food, and to know the content of formalin, borax and rhodamin on food recently. The result of the study showed that quick detection instruments of reagent kit tester produced by BioChem laboratory are effective and efficient to detect formalin, borax and rhodamin on food, with limit potent minimal concentration detection were 5 ppm or less, in not more 10 minutes periods. The result of the study also showed that formalin borax and rhodamin still can be found in many kinds of food distributed in area of Malang.

A Study on Brown Seaweed Therapy ( Sargassum sp.) toward MDA Levels and Histological Improvement on Rat Foot Suffering Rheumatoid Arthritis Fauziah Fauziah; Aulanni'am Aulanni'am; Chanif Mahdi
The Journal of Pure and Applied Chemistry Research Vol 2, No 3 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Rheumatoid arthritis (AR), an autoimun disease, is characterized by the inflammation in the joint area caused an excessive of free radicals. An excessive of free radicals in the body cause oxidative stress, that increasing levels of malondialdehyde (MDA) as an indicator of lipid peroxidation and the decreasing levels of anti-oxidants. The treated with extract of brown seaweed (Sargassum sp.) intended to find out the MDA levels in serum and the histological of the foot joints rheumatoid arthritis rats. Malondialdehyde levels are determined through a TBA test (Thio Barbituric acid), meanwhile the histological of the rat foot joints was determined by Hematoxylen-Eosin staining (HE). The results showed the brown seaweed extract therapy (Sargassum sp.) was significantly (p <0.01) reduce levels of malondialdehyde (MDA) in the serum of 21,24% and improving histological foot joints rheumatoid arthritis rats.

Effect of D-Alpha Tocopherol Therapy towards Malondialdehyde Level and Histology of Kidney in Rattus norvegicus with MLD-STZ Induction Marissa Agnestiansyah Mahardhian; Aulanni’am Aulanni'am; Chanif Mahdi
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Diabetic Nephropathy is a kidney disease which occurs due to complication of diabetes mellitus as a consequence of the damage of the kidney endothelial cells. Hyperglicemia condition in patients with diabetes mellitus that induces an oxidative stress, were related to endothelial cell damage. Oxidative stress as a result of hyperglycemia will activate a number of signal transduction pathways resulting in increase of free radicals. D-alpha tocopherol as one of antioxidant substance, that can act as an inhibitor of free radical chain reactions, play an important role in the reduction of the oxidative stress effect. Effect of D-alpha-tocopherol in reducing oxidative stress is identified by the levels of malondialdehyde (MDA) in kidney and histology of kidney. This study used five groups mice; they were a control group, a diabetic group which was induced with MLD-STZ, and a therapeutic groups with a varieties doses of D-alpha tocopherol (100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW). The results showed that the D-alpha tocopherol was able to reduce the levels of malondialdehyde (MDA) and repair the histology of kidney of mice induced by MLD-STZ.

Peningkatan Konsentrasi Testosteron pada Tikus Akibat Paparan Ekstrak Air Biji Pinang Muslim Akmal; Chanif Mahdi; Aulanni’am -
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.695 KB)

Areca catechu which is known in Indonesia as pinang, contains alkaloids such as arecoline, arecaine,arecaidine, arecolidine, guvacine, guvacoline, and isoguvasine. Arecoline has an ability to change gonadmorfofunction, including shape abnormality of sperm. The aim of this research was to find out the prospectof extract betel nut of A.catechu as male anti fertility agents based on its activity to increase the testosteroneconcentration. Animal models used consisted of 5 groups of 2-3 months male rats (Rattus norvegicus,Wistar strain) and induced for 1 week by water extract of betel nut at the dose of 0, 1, 2, 3, and 4 gram/200gram body weight. Testosterone concentration was determined by ELISA technique. The result showedthat extract betel nut of A. catechu is potential source of natural and beneficial male anti fertility agentsas it can increase the testosterone concentration.

Production and Characterization of Bacillus firmus pectinase Anna Roosdiana; Sasangka Prasetyawan; Chanif Mahdi; Sutrisno Sutrisno
The Journal of Pure and Applied Chemistry Research Vol 2, No 1 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Pectinase is enzyme which functions to hydrolyze pectin become D-galacturonic acid unit. This enzyme is potential in various industries, especially in fruit juice industry. Pectinase can be derived from various microorganisms resulting in different pectinase character. The aims of this research were to determine the optimum condition of pectinase production and to characterize the resulted pectinase including optimum condition of pectinase activity and the influence of metal ion. The optimum condition of pectinase production was carried out by growing Bacillus firmus on basal media containing pectin as inducer at various pH (5, 6, 7, 8, 9, 10), temperature (30, 35, 40, 45, 50) oC and fermentation time (6, 12, 18, 24, 30, 36) hours. while the optimum pectinase activity was done at various pH ( 4, 6, 7, 8, 10 ), temperature (30, 35, 40, 45, 50) oC and reaction time (10, 20, 30, 40, 50) minutes. The influence of Zn2+, Mg2+, K+ at 2-10 mM to pectinase activity were also investigated. The result showed that optimum condition of pectinase production occurred at pH7-8, temperature 40-50 oC and fermentation time 18hours, while the optimum condition of pectinase activity was pH 7, temperature 50 oC and reaction time 30 minutes. The existence of Zn2+, Mg2+, K+ ions affected significantly to pectinase activity. Mg2+ acted as non competitive inhibitor; however K+ and Zn2+ acted as un competitive inhibitor.

Study on iNOS expression and levels of Malondialdehyde in the White Rat (Rattus norvegicus) Exposed with Aggregatibacter actinomycetemcomitans Reviana Setin; Aulanni'am Aulanni'am; Chanif Mahdi
The Journal of Pure and Applied Chemistry Research Vol 2, No 2 (2013)
Publisher : Chemistry Department, The University of Brawijaya

Aggregatibacter actinomycetemcomitant (Aa) is gram-negative bacteria composed of lipopolysaccharides (LPS) in its outer membrane. This bacteria is normally found in dental plaque and periodontal pockets causing parotid gland damage when it is present in excess amounts. LPS is an endotoxin and a complex glycolipid composed of polysaccharides (hydrophilic) and lipid A (hydrophobic) which can induces Reactive Oxygen Species (ROS), and increase the levels of malonaldialdehyde (MDA) and tissue damage. The aims of this research are to find the expression of iNOS parotid gland cell and to determine levels of MDA using thiobarbituric acid (TBA) spectrophotometric test at 533 nm. The results showed a significantly difference (P<0.05) of MDA levels in control rats (3.135 ± 0.0436 ppm) and Aa rats (6.307 ± 0.0473 ppm). iNOS expression is confirmed by the brown spots formed on the parotid gland, affording the enhancements of iNOS expression in Aa rats and control rats of 2.292 ± 0.038 and 0.166 ± 0.003, respectively (P<0.05). Keywords : Aggregatibacter actinomycetemcomitans, Reactive Oxygen Species, malondialdehyde, iNOS

Visualization of Dengue Hemorrhagic Fever (DHF) Spread Mapping at Mitra Sehat Hospital, Situbondo Chanif Mahdi; Malikal Bulgis; Nadhia Rossa Permatasari
Formosa Journal of Multidisciplinary Research Vol. 3 No. 6 (2024): June 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/fjmr.v3i6.9466

Dengue Fever (DF) is a contagious disease caused by the dengue virus transmitted through the bite of Aedes Aegypti mosquitoes and sporadically always becomes an Extraordinary Incident (KLB) every year. Geographic Information System (GIS) is one of the tools that can be used to convey information about the spread of the disease and help analyze the condition of an area against the disease to determine the actions that must be taken to handle the disease. To produce this web-based Geographic Information System, data on Dengue Hemorrhagic Fever cases at Mitra Sehat Hospital are needed. This web-based Geographic Information System begins with data collection, followed by program development using the Google Form platform for data entry, Google Spreadsheet for data collection, and Google Data Studio for data visualization to make it easier to understand.

Co-Authors Anna Roosdiana Aulanni'am, Aulanni'am Aulanni’am - Fauziah Fauziah Malikal Bulgis Marissa Agnestiansyah Mahardhian Muslim Akmal Nadhia Rossa Permatasari Reviana Setin Sasangka Prasetyawan Sutrisno Sutrisno

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