Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrotechnology Research Journal
Supyani Supyani
Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
HYPOVIRULENT ISOLATES OF FUSARIUM COLLECTED FROM CHILI CROPS IN BOYOLALI REGENCY, CENTRAL JAVA, INDONESIA Supyani, Supyani; Widadi, Sri
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Fusarium, a genus of filamentous fungi, has many species which serving as important pathogens to many diseases in crops. Till today, there have not been effective and efficient control methods for such fungi. Recently, scientists agree that application of biological agents is a tactful choice. Development of hypovirulent strains of fungus as biocontrol agents is very limited. This research was aimed to find hypovirulent isolates of Fusarium from field as biological agents. A hundred isolates of Fusarium from chili were collected in Boyolali, Central Java. Morphological characterization revealed that isolates performed varied colony phenotypes. Based on colony phenotype pattern, isolates were classified into five groups. From each group, one hypovirulent isolate was selected based on colony growth rate on potato dextrose agar media. The selected hypovirulent isolates were used for virulence assay in apple. The result showed that there were four hypovirulent isolates i.e.: B6, C15, D19, and E20 isolates. Total RNA extraction of the identified hypovirulent isolates revealed the existence of viral RNA in C15 isolate. Based on the existence of viral RNA in C15 isolate, the hypovirulent traits were due to mycoviral infection, whereas the hypovirulent traits performed by the other three were due to genetic factors. Keywords: biological control agent, Fusarium, hypovirulent isolate, virulence assay 
Efektivitas Ekstrak Daun Bunga Pukul Empat untuk Pengendalian Penyakit Mosaik Kacang Panjang Supyani, Supyani; Widadi, Sri; Jamil, Wahyu Hidayah Andriyani
Agrotechnology Research Journal Vol 1, No 1 (2017): AGROTECHNOLOGY RESEARCH JOURNAL
Publisher : Perhimpunan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (566.522 KB) | DOI: 10.20961/agrotechresj.v1i1.18870

Abstract

Production of  long beans has declined, caused by mosaic virus infection. The disease was still difficult to be control. This study aims to determine role and to measure the effectivity of Mirabilis jalapa leaf extract to inhibit long bean mosaic disease, based on the concentration of extract and time of  application. The Research was arranged by Completely Randomized Design (CRD) with two factors, time of application, and concentration of M. jalapa leaf extract. Data were analyzed  using F  test and DMRT (Duncans Multiple Range Test) at level of 5%. The results showed that application of M. jalapa leaf extract 25% one day before inoculation was best treatment to delay the emergence of the mosaic disease symptoms up to 32 days and able to increase the average weight of pods up to 104,45 grams. Soaking the seeds to extract 25% were able to suppress the virus up to 96,93%. The Application of M. jalapa leaf extract 50% with symptomatic leaf sap of mosaic simultaneously showed highest in plant’s fresh weight and biomass weight. Concentration of M. jalapa  leaf extract 25%, which applied one day before inoculation was most effective to control the mosaic virus disease.
Identification of plant growth promoting rhizobacteria around Pulang Pisang Food Estate, Central Kalimantan, Indonesia Andriani, Lutfi Tri; Poromarto, Susilo Hambeg; Supyani, Supyani; Purwanto, Edi; Hadiwiyono, Hadiwiyono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225169-178

Abstract

Plant growth-promoting bacteria are well known as biostimulants, biofertilizers, bioprotectants, and biodegraders. The Pulang Pisau Food Estate is an Indonesian government program aimed at increasing rice crop production to achieve sustainable food self-sufficiency. Research on rhizobacteria in the Pulang Pisau Food Estate area is still relatively limited. In this study, we conducted in vitro assays to evaluate the potential of indigenous bacterial isolates from the Pulang Pisau Food Estate as plant growth-promoting rhizobacteria (PGPR). The study focused on four bacterial isolates, which were tested for plant growth-promoting traits including phosphate solubilization, indole-3-acetic acid (IAA) production, effects on rice seed germination, and detached leaf assays to assess the ability of rhizobacteria to inhibit bacterial pathogens. The results of 16S rRNA gene identification suggested that isolates UNS-P1, UNS-P3, and UNS-R1 were closely related to Bacillus cereus, while one previously identified isolate (UNS-R2) was confirmed as Bacillus subtilis. All bacterial strains were able to produce IAA, while only one isolate demonstrated the ability to solubilize phosphate. In the germination test, no significant differences were observed in root length, but a significant difference in shoot (plant) height was detected. Bacillus subtilis (UNS-R2), at a 10³ dilution, resulted in significantly greater plant height compared to other treatments. Among the four bacterial isolates, only one showed the ability to inhibit the pathogen Pantoea ananatis. These results suggest that indigenous bacterial isolates from the Pulang Pisau Food Estate have potential as plant growth-promoting rhizobacteria (PGPR) and may contribute to enhancing plant growth and serve as biocontrol agents against P. ananatis.
Co-Authors . Hadiwiyono Andriani, Lutfi Tri Edi Purwanto Jamil, Wahyu Hidayah Andriyani Poromarto, Susilo Hambeg Sri Widadi Sri Widadi