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All Journal Jurnal Fitopatologi Indonesia AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia
Supyani Supyani
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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HYPOVIRULENT ISOLATES OF FUSARIUM COLLECTED FROM CHILI CROPS IN BOYOLALI REGENCY, CENTRAL JAVA, INDONESIA Supyani, Supyani; Widadi, Sri
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Fusarium, a genus of filamentous fungi, has many species which serving as important pathogens to many diseases in crops. Till today, there have not been effective and efficient control methods for such fungi. Recently, scientists agree that application of biological agents is a tactful choice. Development of hypovirulent strains of fungus as biocontrol agents is very limited. This research was aimed to find hypovirulent isolates of Fusarium from field as biological agents. A hundred isolates of Fusarium from chili were collected in Boyolali, Central Java. Morphological characterization revealed that isolates performed varied colony phenotypes. Based on colony phenotype pattern, isolates were classified into five groups. From each group, one hypovirulent isolate was selected based on colony growth rate on potato dextrose agar media. The selected hypovirulent isolates were used for virulence assay in apple. The result showed that there were four hypovirulent isolates i.e.: B6, C15, D19, and E20 isolates. Total RNA extraction of the identified hypovirulent isolates revealed the existence of viral RNA in C15 isolate. Based on the existence of viral RNA in C15 isolate, the hypovirulent traits were due to mycoviral infection, whereas the hypovirulent traits performed by the other three were due to genetic factors. Keywords: biological control agent, Fusarium, hypovirulent isolate, virulence assay 
Aplikasi Gelombang Mikro sebagai Pengendali Cendawan Patogen Terbawa Benih Kedelai Raden Sujayadi; Supyani Supyani; Edi Purwanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 6 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.45 KB) | DOI: 10.14692/jfi.13.6.191

Abstract

One of the obstacles in providing high quality soybean (Glycine max) seed is the infection of seed-borne fungal pathogens. Micro wave treatment is one of the alternative methods to control the seed-borne pathogens effectively but it needs to be further developed. This research consisted of two stages. The first stage was aimed to determine the best soybean seed moisture content in maintaining seed physiological quality after exposing to micro wave. The second stage was aimed to determine the most effective duration of micro wave exposure to reduce the rate of infection of seed-borne fungal pathogens using the best moisture content resulted from the first stage. The experiment  on seed moisture indicated that 9.20% was the best seed moisture content in maintaining the physiological quality when the seed was exposed to micro wave.  Furthermore, micro wave exposure of 60 seconds was able to control Aspergillus flavus, while the exposure of  80 seconds was able to control Fusarium sp., Curvularia sp., and A. niger.
Mikovirus, Pengembangannya sebagai Agens Pengendali Hayati Supyani Supyani
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.17874

Abstract

Mycoviruses are viruses that infect fungus. In plant pathogenic fungi, mycoviruses infection may cause hypovirulence. Therefore, mycoviruses could be developed as biological control agents against the plant pathogenic fungi. Abroad, mycoviruses have long been studied and developed into biological control agents. So far, many mycoviruses have been and are being developed as biological control agents. One that has been successfully manipulated is the Cryphonectria hypovirus 1 to control blight disease on chestnut trees (Castanea dentata). In Indonesia, in brief, mycoviruses has not been sufficiently investigated. This article is aimed to introduce mycoviruses in Indonesia, as well as to inform that a study on mycoviruses has been underway in the country IntisariMikovirus adalah virus yang menginfeksi jamur. Pada jamur patogen tumbuhan, infeksi mikovirus dapat menyebabkan hipovirulen. Dengan demikian mikovirus dapat dikembangkan sebagai agens pengendali hayati terhadap jamur patogen tumbuhan. Di luar negeri mikovirus sudah sejak lama dikaji, diantaranya adalah untuk dikembangkan sebagai agens pengendali hayati. Sampai sekarang sudah banyak mikovirus yang telah/sedang dikembangkan sebagai agens pengendali hayati. Salah satu yang sudah berhasil adalah Cryphonectria hypovirus 1 untuk mengendalikan penyakit hawar kastanye (Castanea dentata). Di tanah air, mikovirus dapat dibilang belum dikaji secara memadai. Artikel ini bertujuan untuk memperkenalkan mikovirus di tanah air, sekaligus menginformasikan rintisan kajian mikovirus yang telah dilakukan di tanah air.
HYPOVIRULENT ISOLATES OF FUSARIUM COLLECTED FROM CHILI CROPS IN BOYOLALI REGENCY, CENTRAL JAVA, INDONESIA Supyani Supyani; Sri Widadi
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i1.467

Abstract

Fusarium, a genus of filamentous fungi, has many species which serving as important pathogens to many diseases in crops. Till today, there have not been effective and efficient control methods for such fungi. Recently, scientists agree that application of biological agents is a tactful choice. Development of hypovirulent strains of fungus as biocontrol agents is very limited. This research was aimed to find hypovirulent isolates of Fusarium from field as biological agents. A hundred isolates of Fusarium from chili were collected in Boyolali, Central Java. Morphological characterization revealed that isolates performed varied colony phenotypes. Based on colony phenotype pattern, isolates were classified into five groups. From each group, one hypovirulent isolate was selected based on colony growth rate on potato dextrose agar media. The selected hypovirulent isolates were used for virulence assay in apple. The result showed that there were four hypovirulent isolates i.e.: B6, C15, D19, and E20 isolates. Total RNA extraction of the identified hypovirulent isolates revealed the existence of viral RNA in C15 isolate. Based on the existence of viral RNA in C15 isolate, the hypovirulent traits were due to mycoviral infection, whereas the hypovirulent traits performed by the other three were due to genetic factors.
Co-Authors . Hadiwiyono Budi Tjahjono Edi Purwanto Jamil, Wahyu Hidayah Andriyani Lulu'il Maknun Raden Sujayadi Sri Widadi Sri Widadi Sri Widadi