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HYPOVIRULENT ISOLATES OF FUSARIUM COLLECTED FROM CHILI CROPS IN BOYOLALI REGENCY, CENTRAL JAVA, INDONESIA Supyani, Supyani; Widadi, Sri
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Fusarium, a genus of filamentous fungi, has many species which serving as important pathogens to many diseases in crops. Till today, there have not been effective and efficient control methods for such fungi. Recently, scientists agree that application of biological agents is a tactful choice. Development of hypovirulent strains of fungus as biocontrol agents is very limited. This research was aimed to find hypovirulent isolates of Fusarium from field as biological agents. A hundred isolates of Fusarium from chili were collected in Boyolali, Central Java. Morphological characterization revealed that isolates performed varied colony phenotypes. Based on colony phenotype pattern, isolates were classified into five groups. From each group, one hypovirulent isolate was selected based on colony growth rate on potato dextrose agar media. The selected hypovirulent isolates were used for virulence assay in apple. The result showed that there were four hypovirulent isolates i.e.: B6, C15, D19, and E20 isolates. Total RNA extraction of the identified hypovirulent isolates revealed the existence of viral RNA in C15 isolate. Based on the existence of viral RNA in C15 isolate, the hypovirulent traits were due to mycoviral infection, whereas the hypovirulent traits performed by the other three were due to genetic factors.Â Keywords: biological control agent, Fusarium, hypovirulent isolate, virulence assayÂ

Aplikasi Gelombang Mikro sebagai Pengendali Cendawan Patogen Terbawa Benih Kedelai Raden Sujayadi; Supyani Supyani; Edi Purwanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 6 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

One of the obstacles in providing high quality soybean (Glycine max) seed is the infection of seed-borne fungal pathogens. Micro wave treatment is one of the alternative methods to control the seed-borne pathogens effectively but it needs to be further developed. This research consisted of two stages. The first stage was aimed to determine the best soybean seed moisture content in maintaining seed physiological quality after exposing to micro wave. The second stage was aimed to determine the most effective duration of micro wave exposure to reduce the rate of infection of seed-borne fungal pathogens using the best moisture content resulted from the first stage. The experiment on seed moisture indicated that 9.20% was the best seed moisture content in maintaining the physiological quality when the seed was exposed to micro wave. Furthermore, micro wave exposure of 60 seconds was able to control Aspergillus flavus, while the exposure of 80 seconds was able to control Fusarium sp., Curvularia sp., and A. niger.

Mikovirus, Pengembangannya sebagai Agens Pengendali Hayati Supyani Supyani
Jurnal Perlindungan Tanaman Indonesia Vol 21, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.17874

Mycoviruses are viruses that infect fungus. In plant pathogenic fungi, mycoviruses infection may cause hypovirulence. Therefore, mycoviruses could be developed as biological control agents against the plant pathogenic fungi. Abroad, mycoviruses have long been studied and developed into biological control agents. So far, many mycoviruses have been and are being developed as biological control agents. One that has been successfully manipulated is the Cryphonectria hypovirus 1 to control blight disease on chestnut trees (Castanea dentata). In Indonesia, in brief, mycoviruses has not been sufficiently investigated. This article is aimed to introduce mycoviruses in Indonesia, as well as to inform that a study on mycoviruses has been underway in the country IntisariMikovirus adalah virus yang menginfeksi jamur. Pada jamur patogen tumbuhan, infeksi mikovirus dapat menyebabkan hipovirulen. Dengan demikian mikovirus dapat dikembangkan sebagai agens pengendali hayati terhadap jamur patogen tumbuhan. Di luar negeri mikovirus sudah sejak lama dikaji, diantaranya adalah untuk dikembangkan sebagai agens pengendali hayati. Sampai sekarang sudah banyak mikovirus yang telah/sedang dikembangkan sebagai agens pengendali hayati. Salah satu yang sudah berhasil adalah Cryphonectria hypovirus 1 untuk mengendalikan penyakit hawar kastanye (Castanea dentata). Di tanah air, mikovirus dapat dibilang belum dikaji secara memadai. Artikel ini bertujuan untuk memperkenalkan mikovirus di tanah air, sekaligus menginformasikan rintisan kajian mikovirus yang telah dilakukan di tanah air.

HYPOVIRULENT ISOLATES OF FUSARIUM COLLECTED FROM CHILI CROPS IN BOYOLALI REGENCY, CENTRAL JAVA, INDONESIA Supyani Supyani; Sri Widadi
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i1.467

Fusarium, a genus of filamentous fungi, has many species which serving as important pathogens to many diseases in crops. Till today, there have not been effective and efficient control methods for such fungi. Recently, scientists agree that application of biological agents is a tactful choice. Development of hypovirulent strains of fungus as biocontrol agents is very limited. This research was aimed to find hypovirulent isolates of Fusarium from field as biological agents. A hundred isolates of Fusarium from chili were collected in Boyolali, Central Java. Morphological characterization revealed that isolates performed varied colony phenotypes. Based on colony phenotype pattern, isolates were classified into five groups. From each group, one hypovirulent isolate was selected based on colony growth rate on potato dextrose agar media. The selected hypovirulent isolates were used for virulence assay in apple. The result showed that there were four hypovirulent isolates i.e.: B6, C15, D19, and E20 isolates. Total RNA extraction of the identified hypovirulent isolates revealed the existence of viral RNA in C15 isolate. Based on the existence of viral RNA in C15 isolate, the hypovirulent traits were due to mycoviral infection, whereas the hypovirulent traits performed by the other three were due to genetic factors.

Identification of plant growth promoting rhizobacteria around Pulang Pisang Food Estate, Central Kalimantan, Indonesia Andriani, Lutfi Tri; Poromarto, Susilo Hambeg; Supyani, Supyani; Purwanto, Edi; Hadiwiyono, Hadiwiyono
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225169-178

Plant growth-promoting bacteria are well known as biostimulants, biofertilizers, bioprotectants, and biodegraders. The Pulang Pisau Food Estate is an Indonesian government program aimed at increasing rice crop production to achieve sustainable food self-sufficiency. Research on rhizobacteria in the Pulang Pisau Food Estate area is still relatively limited. In this study, we conducted in vitro assays to evaluate the potential of indigenous bacterial isolates from the Pulang Pisau Food Estate as plant growth-promoting rhizobacteria (PGPR). The study focused on four bacterial isolates, which were tested for plant growth-promoting traits including phosphate solubilization, indole-3-acetic acid (IAA) production, effects on rice seed germination, and detached leaf assays to assess the ability of rhizobacteria to inhibit bacterial pathogens. The results of 16S rRNA gene identification suggested that isolates UNS-P1, UNS-P3, and UNS-R1 were closely related to Bacillus cereus, while one previously identified isolate (UNS-R2) was confirmed as Bacillus subtilis. All bacterial strains were able to produce IAA, while only one isolate demonstrated the ability to solubilize phosphate. In the germination test, no significant differences were observed in root length, but a significant difference in shoot (plant) height was detected. Bacillus subtilis (UNS-R2), at a 10³ dilution, resulted in significantly greater plant height compared to other treatments. Among the four bacterial isolates, only one showed the ability to inhibit the pathogen Pantoea ananatis. These results suggest that indigenous bacterial isolates from the Pulang Pisau Food Estate have potential as plant growth-promoting rhizobacteria (PGPR) and may contribute to enhancing plant growth and serve as biocontrol agents against P. ananatis.

Evaluasi Efektivitas Mikoriza dan Pupuk Organik terhadap Pengendalian Serangan Fusarium oxysporum f.sp. Cepae pada Bawang Putih Supyani, Supyani; Septariani, Dwiwiyati Nurul; Haqiki, Zahra Tazkiyatun; Hadiwiyono, Hadiwiyono
Agrosains : Jurnal Penelitian Agronomi Vol 25, No 2 (2023): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v25i2.71308

The main problem in garlic cultivation was root rot disease caused by Fusarium oxysporum f.sp. cepae. Environmentally friendly control following sustainable agriculture can be used to increase plant resistance by providing biological fertilizers such as mycorrhizae and organic fertilizers such as compost and husk charcoal. This research aimed to evaluate the effectiveness of the application of mycorrhizae and organic fertilizers against Fusarium oxysporum f.sp. cepae. The research was conducted from June to November 2021 at the Tawangmangu Horticultural Seed Garden in Jl. Raya Tawangmangu, Beji, Tawangmangu, Karanganyar. Laboratory test was conducted from December 2021 to January 2022 at Laboratory of Plant Pests and Diseases and Laboratory of Soil Chemistry, Faculty of Agriculture, Sebelas Maret University. The method used was completely randomized design with a combination of mycorrhizae treatment (2 gr plant -1), compost fertilizer (16 ton ha-1), and husk charcoal fertilizer (6 ton ha-1). The data obtained were analyzed using analysis of variance and then continued with the Duncan’s Multiple Range Test. The results showed that mycorrhizae gave 22.95% effectiveness in wilting control and 20.93% in tuber rot control. Organic fertilizers provide the wilting effectiveness control by 9.27% (compost) and 8.06% (husk charcoal), as well as tuber rot effectiveness control by 27.91% (compost) and 25.58% (husk charcoal). The combination of mycorrhizae and organic fertilizers gave the highest wilt control effectiveness of 26.07% in the combination of mycorrhizae, compost, and husk bran. The effectiveness of tuber rot control was found in the combination of Mycorrhizae and compost, which was 34.88%.

Efficacy of Some Packages of Fungicide for Seed Treatment and Spraying Corn in the Fields against Downy Mildew Siregar, Ichwanul Malik; Widono, Salim; Faruk, Mohammed Iqbal; Supyani, Supyani
Agrotechnology Research Journal Vol 8, No 2 (2024): Agrotechnology Research Jurnal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v8i2.94044

This study aimed to identify the most effective combination of seed treatments and fungicide applications to control downy mildew on corn seeds. It also evaluated the efficacy of various fungicides in suppressing the spread of downy mildew. The research was conducted from October 2021 to January 2022 in Banggle Village, Kanigoro District, Blitar Regency, Central Java Province. The methodology employed a randomized complete block design, with treatment factors including seed treatment and fungicide spraying. Four treatment packages were utilized: one control group with no seed treatment or fungicide application and three others as comparators. Each treatment was replicated four times. The active ingredients used for seed treatment and field spraying included Dimethomorph, Pyraclostrobin, Oxathiapiprolin, and Fenamidone. The results indicated that downy mildew on corn emerged during the fourth week after planting. Corn treated with the fungicide packages exhibited significantly better outcomes compared to the control group. The most effective treatment package involved seed treatment with Dimethomorph and Pyraclostrobin, followed by spraying with Dimethomorph on the seventh day after planting, Oxathiapiprolin on the fourteenth day, and Dimethomorph once again on the twenty-first day after planting. This combination achieved the highest efficacy index of 70.67%.

Potential of Trichocompost to Improve Organic Garlic Production in Fusarium Wilt–Endemic Fields in Tawangmangu, Central Java Poromarto, Susilo Hambeg; Supyani, Supyani; Widono, Salim; Septariani, Dwiwiyati Nurul; Hadiwiyono, Hadiwiyono; Enggartiasto, Argha Hyta Dimas
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.111410

Garlic is a high-value commodity in Tawangmangu, one of its major production centers in Central Java. However, productivity in this region is seriously affected by Fusarium, the pathogen causes basal rot and wilting in the upper parts of the plant. The disease known as basal rot or Fusarium wilt, with the disease intensity in the field can exceeding 60%. Currently, chemical control remains the primary management strategies, which in organic farming, this practice is not acceptable. Therefore, environmentally friendly alternatives are required, such as using organic fertilizers, biological control agent like Trichoderma or combination. Trichoderma is also known as a cellulolytic microbe, which can enhance the composting process and produce Trichompost with improved quality. Field experiment results indicate that Trichocompost (TC) performs better compared to compost (C), Trichoderma (T), or untreated treatment. This findings suggest that garlic treated with Trichocompost and organic fertilizer show better growth with lower wilting intensity. Therefore, there is an indication that increased plant growth can reduce the intensity of Fusarium wilt in garlic.

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