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All Journal International Journal of Renewable Energy Development Jurnal Matematika & Sains Journal of Engineering and Technological Sciences Jurnal Kimia Riset Berkala Penelitian Hayati
Akhmaloka Akhmaloka
Departemen Kimia, FMIPA, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Energy Engineering Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology

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Journal : Journal of Engineering and Technological Sciences

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Ribotyping Identification of Thermophilic Bacterium from Papandayan Crater Akhmaloka, Akhmaloka; Suharto, A.; Nurbaiti, S.; Tika, I. N.; Warganegara, F. M.
Journal of Engineering and Technological Sciences Vol 38, No 1 (2006)
Publisher : ITB Journal Publisher, LPPM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.31 KB) | DOI: 10.5614/itbj.eng.sci.2006.38.1.1

Abstract

A few thermophilic bacteria were isolated from a hot spring located in Papandayan Crater, Garut. One of the organisms showed a well growth at temperature of up to 80 oC. Chromosomal DNA from the organism was isolated and used to amplify 16S rRNA gene fragment. The gene was amplified by a set of universal primers (27F and 1492R) resulting in a 1.5 kb DNA fragment. The gene was cloned and sequenced. The phylogenetic tree, homological analysis, and detailed comparison of the sequences showed that 16S rRNA gene sequence of the Papandayan isolate is unique compared to other known strains, however the sequence had closest similarities with Bacillus caldolyticus and Bacillus caldotenax.
Ribotyping Identification of Thermophilic Bacterium from Papandayan Crater Akhmaloka Akhmaloka; A. Suharto; S. Nurbaiti; I. N. Tika; F. M. Warganegara
Journal of Engineering and Technological Sciences Vol. 38 No. 1 (2006)
Publisher : Institute for Research and Community Services, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/itbj.eng.sci.2006.38.1.1

Abstract

A few thermophilic bacteria were isolated from a hot spring located in Papandayan Crater, Garut. One of the organisms showed a well growth at temperature of up to 80 oC. Chromosomal DNA from the organism was isolated and used to amplify 16S rRNA gene fragment. The gene was amplified by a set of universal primers (27F and 1492R) resulting in a 1.5 kb DNA fragment. The gene was cloned and sequenced. The phylogenetic tree, homological analysis, and detailed comparison of the sequences showed that 16S rRNA gene sequence of the Papandayan isolate is unique compared to other known strains, however the sequence had closest similarities with Bacillus caldolyticus and Bacillus caldotenax.
Co-Authors A. Suharto A. Suharto AFAF BAKTIR Ami Soewandi J.S. Arina, Linea Alfa Dewi, Arinta Elvi Restiawaty F Madayanti, F F. M. Warganegara F. M. Warganegara Gani, Kindi Pyta Garaika H. Sutedjo Hadi Sutedjo I. N. Tika I. N. Tika Kurniawati, Katarina Ika M. Sindumarta Meray, Nishia Waya Ni Nyoman Tri Puspaningsih P Aditiawati P. E. Susilowati R. Hertadi RUKMAN HERTADI S. Nurbaiti S. Nurbaiti Safika S, Safika Senam Senam Suharti Suharti Y. Sriwulan M. Yogi Wibisono Budhi