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All Journal Program Kreativitas Mahasiswa - Penelitian Indonesia Chimica Acta
Ali Muhakim
Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Hasanuddin
Chemical Engineering, Chemistry & Bioengineering

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KARAKTERISASI DAN UJI BIOAKTIVITAS SENYAWA KIMIA ANTI TUBERCULOSIS (TBC) PADA SPONS PETROSIA ALFIANI DARI PERAIRAN SELAT MAKASSAR Rahman, Abd.; Aminah, Ibtisamatul; Muhakim, Ali
Program Kreativitas Mahasiswa - Penelitian PKM-P 2013
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.491 KB)

Abstract

This study is intended to take advantage of the chemical compounds contained in sponge species Petrosia alfiani as anti tuberculosis (TB). This study through the stages isolation, extraction, identification and bioactivity testing of chemical compounds Petrosia alfiani sponge. In this study produce chloroform extract as much as 20 g of solid resulting from 20 Kg wet sponge and also produce 4 pieces of pure compounds are not yet known its name.Keyword: Petrosia alfiani sponge, isolation, identication, bioactivity testing, chemical compounds.
The Toxicity and Cytotoxicity Test of Anticancer Peptides Isolated from Symbiotic Bacteria Asmi, Nur; Ahmad, Ahyar; Natsir, Hasnah; Karim, Harningsih; Muhakim, Ali; Khairunnur, Siti
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Vol. 18 No. 1 (2025): Volume 18, No 1: June 2025
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70561/ica.v18i1.43925

Abstract

The purpose of this research was to evaluate the toxicity and cytotoxicity of the fragment peptide from symbiotic bacteria in the lung cancer LK-2 cell line, compared normal M5S cell line. Protein hydrolyzates were separated using Molecular Weight Cut Off (MWCO) to isolate peptide fragments. Fragments were categorized as ≤ 5 kDa, > 5-10 kDa, and > 10 kDa to evaluate their toxicity through the Brine Shrimp Lethality Test (BSLT) method and to study cytotoxicity effects and proliferation in LK-2 lung cancer cells against normal M5S cells. MTT assay was used to analyze the percentage of living cells and cell growth. The Selectivity Index (SI) equation was applied to determine selectivity. Findings indicated that the peptide fragment ≤ 5 kDa had the highest toxicity, with an LC50 of 8.15 µg/ml. The number of LK-2 cells that died increased when exposed to peptide fragments of sizes ≤ 5 kDa, > 5-10 kDa, and > 10 kDa over 16 hours. Moreover, cell growth in LK-2 cells decreased after 16 hours, while there was no significant decline (P < 0.05) in M5S cells compared to the controls. The peptide fragments with molecular weights ≤ 5 kDa and > 5-10 kDa demonstrated the highest selectivity, resulting in an SI value of 3. These results suggest that the selected peptide fragments may serve effectively as anticancer agents. This study highlights the potential of peptides from symbiotic bacteria as possible treatments for cancer, particularly lung cancer.
Co-Authors Abd. Rahman Ahyar Ahmad Harningsih Karim Hasnah Natsir Ibtisamatul Aminah Khairunnur, Siti Nur Asmi