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All Journal Journal of Tropical Biodiversity and Biotechnology Jurnal Dinamika Pengabdian (JDP) Indonesia Chimica Acta Indonesian Journal of Chemistry Communications in Science and Technology SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Chemistry Indonesian Journal of Chemical Research Jurnal Pengabdian Masyarakat Borneo Abdimas Galuh : Jurnal Pengabdian Kepada Masyarakat Indobiosains Molekul: Jurnal Ilmiah Kimia
Hasnah Natsir
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10 Tamalanrea, Makassar 90245, South Sulawesi
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Isolation and Identification of Cellulolytic Bacteria from Gut of Horn Beetle Larvae (Oryctes rhinoceros L.) Riskawati, Riskawati; Natsir, Hasnah; Dali, Seniwati; Baharuddin, Maswati
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6848

Abstract

The horn beetle larvae (Oryctes rhinoceros L.) contain symbiotic bacteria that are used to digest and degrade cellulose as food so that it has the potential to produce cellulase enzymes. This study aims to isolate, characterize and identify microbial symbionts from horn beetle larvae that have the potential to produce cellulase enzymes.The methods in this study include morphology and physiology identification of bacteria, qualitative and quantitative activity tests and species determination using 16S rRNA sequencing technique. Based on the results of morphological observations, five bacterial isolates were taken which has the potential as a cellulase producer is indicated by the presence of a clear zone that is produced when a qualitative test is carried out using congo red staining with different cellulolytic indices. Based on the quantitative bacterial activity test using UV-Vis, the highest activity was found in PES3 isolates at 1.62 x 10-2 and PES5 at 1.61 x 10-2. Species determination results found that PES3 isolates belonged to the genus Acinetobacter and PES5 belonged to Pseudomonas. In addition to the isolates obtained for the environment and the industrial sector, cellulolytic bacteria can provide added value such as hydrolyze cellulose waste into alternative fuels.
Chitinase Enzyme-Producing Endophytic Bacterias From the Roots of the Plant Gembolo (Dioscorea bulbifera): Isolation, Characterization and its Potential as an Antifungal Agent Yunita, Vivi Alfi; Natsir, Hasnah; Ahmad, Ahyar; Baharuddin, Maswati
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.9422

Abstract

Chitinase is an enzyme of the chitinolytic group that has many roles in agriculture, especially as an antifungal, because chitin is one of the constituent components of the fungal cell wall. This study aimed to isolate and identify endophytic bacteria from gembolo (Dioscorea bulbifera) roots and to characterize the chitinase enzyme from these endophytic bacteria to be used as an antifungal. Isolation and identification of gembolo plant root endophytic bacteria using PCR method of 16S rRNA gene amplification and sequencing. Characterization of the chitinase enzyme produced includes determining of optimum pH, temperature, and substrate UV-Vis spectrophotometer. Antagonistic test of the chitinase enzyme and endophytic bacterial isolates (isolate K4) Fusarium oxysporum. The results showed that bacterial isolate K4 had with chitinolytic index of 2.45 mm. Electrophoresis results with PCR 16s rRNA gene; the length of the amplified fragment is the position of 1300 bp. By doing the BLAST process in GenBank, the bacterial isolate has 97.93% similarity with Enterobacter cloacae. Then, this endophytic bacteria is called Enterobacter cloacae K4-G. This bacterium produced chitinase enzyme reaching maximum chitinase activity at the 38 hours with an activity of 0.0312 U/mL. The chitinase characterization results of E. cloacae K4-G showed that the optimum conditions were reached at at pH 6, temperature 45 ᵒC, and 2.5% substrate with a chitinase activity value of 0.2467 U/mL. Chitinase enzyme and bacteria Enterobacter cloacae K4-G can inhibit the growth of the fungus Fusarium oxysporum. Therefore, Chitinase from Enterobacter cloacae K4-G can be used as an antifungal pathogen in plants.
Bioactivity and Metabolites Compounds of Medicinal Plants Endophytic Fungi in Indonesia Sukmawaty, Eka; Karim, Abdul; dwyana, Zarsawaty; Natsir, Hasnah; Karim, Harningsih; Ahmad, Ahyar
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.79070

Abstract

Indonesia is rich in diversity of medicinal plants, vital in traditional medicine and the pharmaceutical industry. However, overharvesting, along with population growth, land use changes, deforestation, and climate change, endanger these plants. This review investigates the potential of endophytic endosymbiont as an alternative. These endosymbionts can synthesise bioactive compounds similar to those found in medicinal plants.  This study compiled data from various sources on endophytic fungi and their bioactivity. The review aims to categorise Indonesian medicinal plants, to identify their associated endophytic fungi from different plant parts, and to assess their bioactivity. The results revealed numerous medicinal plant families and a variety of endophytic fungi isolated from fruits, leaves, twigs, bark, roots, and rhizomes. These fungi exhibited bioactivities, including antioxidant, anticancer, antidiabetic, and antimicrobial effects, with metabolites such as alkaloids, flavonoids, peptides, phenols, polyketides, quinones, steroids, and terpenoids. Fusarium and Colletotrichum were the most common endophytic fungi found. Notably, the biological activity was consistent among endophytic fungi from various host organs, but variations were observed according to the host's geographical origin. This suggests that Indonesia's diverse geography influences metabolite production and activity. However, the same host plant may harbour different species in distinct organs. These findings indicate that endophytic fungi within medicinal plants represent a promising source of bioactive compounds for future Indonesian medicine production. Future research should explore metabolite compounds and bioactivity across different geographical regions. 
Produksi Oligomer Kitosan dari Limbah Udang Windu (Panaeus monodon) Menggunakan Enzim Kitosanase dari Isolat Bakteri Klebsiella sp. Sarni, Sarni; Natsir, Hasnah; Dali, Seniwati
Indonesian Journal of Chemical Research Vol 3 No 2 (2016): Edisi Bulan Januari (Edition For January)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2016.3-sar

Abstract

Chitosan is a biopolymer that is the main content of D-glucosamine and several parts of N-acetyl-D-glucosamine bound to β- (1-4) glucoside. Chitosan receive special attention as functional biopolymers for applications in various fields. Chitosan is more effectively absorbed into the human body when it gets converted into chitosan oligomer form. Chitosan oligomer is a mixture of oligomers of D-glucosamine are formed through a process of severing ties depolymerization of chitosan with β-glycosidic. This study aims to produce chitosan oligomer of waste tiger shrimp (Penaeus monodon) using enzyme kitosanase of bacteria Klebsiella sp. Chitosan oligomer produced by using the enzyme chitosanase at a temperature of 40 °C and pH 8 with the activity of 0.309 U/mL (5,235 U/mg) obtained in the form of a mixture of monomer to octamer, which soluble in acetic acid 0.25% to 0.5%, having intrinsic viscosity decreases with increasing time of incubation is 0.195 (1 hour incubation); 0.9 (incubation 2 hours) and 0.7 (incubation 3 hours) with molecular weight range of 4103.12 g/mol (incubation 1 hour) ; 1483.48 g/mol (incubation 2 hours) and 1065.79 g/mol (incubation 3 hours).
In Vitro and In Silico Assessment of Methanol Extract from Moringa oleifera Seeds as α-Amylase Inhibitor Natsir, Hasnah; A Arfah, Rugaiyah; Arif, Abdur Rahman; Nadir, Muhammad; Anita, Anita; Sartika, Sartika; Rahmi, Nur; Karimah, Aulia
Indonesian Journal of Chemical Research Vol 12 No 2 (2024): Edition for September 2024
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2024.12-has

Abstract

Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia, posing a global health threat. Current diabetes treatments often rely on synthetic drugs with side effects, prompting the search for safer natural alternatives. This study investigated the secondary metabolic compounds in methanol extract from M. oleifera seeds and evaluated their antidiabetic activity. The α-amylase enzyme was analyzed to determine optimal incubation time, pH, and temperature. The antidiabetic activity was assessed via α-amylase inhibition using in vitro and in silico methods. Phytochemical screening revealed the presence of flavonoids, alkaloids, and tannins in the extract. Optimal enzyme conditions were 30 minutes of incubation, pH 6.9, and 25 °C. The extract showed the highest activity at 15% concentration with 67.94% inhibition and an IC50 of 15.38%, compared to acarbose with 41.76% inhibition and an IC50 of 17.89%. In silico analysis indicated that 9-octadecanoic acid (Z) methyl ester had a lower inhibition constant and binding energy (2.67 mM and -3.51 kcal/mol) than acarbose (15.72 mM and -2.46 kcal/mol), suggesting a higher enzyme affinity. These findings suggest that M. oleifera seeds contain compounds with potential as antidiabetic agents.
Green synthesis of copper oxide (CuO) nanoparticles using Anredera cordifolia leaf extract and their antioxidant activity Munandar, Nurharis; Kasim, Syaharuddin; Arfah, Rugaiyah; Basir, Djabal Nur; Hala, Yusafir; Zakir, Muhammad; Natsir, Hasnah
Communications in Science and Technology Vol 7 No 2 (2022)
Publisher : Komunitas Ilmuwan dan Profesional Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21924/cst.7.2.2022.1004

Abstract

Our present study describes the green synthesis of copper oxide nanoparticles (CuONPs) using a leaf extract from Anredera cordifolia (AC). The presence of flavonoids, saponins, and alkaloids in the plant extract was confirmed by phytochemical analysis, and these chemicals can be used as reducing, stabilizing, and capping agents. UV-Vis Spectrophotometry, XRD, FT-IR, and SEM-EDS were used to characterize the CuONPs. The UV-Vis spectroscopy of the biosynthesized CuONPs revealed an absorption peak at 325 nm. The XRD graph also revealed that the Debye-Scherrer formula observed an average crystalline size of around 43.47 nm. EDS analysis confirmed the composition and purity of CuONPs. The presence of functional groups -OH, C=O, and C-O triggers the synthesis of CuONPs, according to FT-IR analysis. Furthermore, the CuONPs antioxidant property inhibited free radicals as antioxidants with an IC50 value of 34.20 g/mL.
Pemberdayaan Masyarakat Kabupaten Pinrang Melalui Pemanfaatan Kelapa, Kacang Tanah dan Kakao (Kalangka) dalam Proses Fermentasi Menjadi Produk Bahan Makanan Permatasari, Nur Umriani; Natsir, Hasnah; Haedar, Nur; Musa, Bulkis; Situmorang, Sarah Victoria Octovianni; Anggriani, Dhea Faraditha
Abdimas Galuh Vol 6, No 2 (2024): September 2024
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v6i2.15462

Abstract

Kabupaten Pinrang adalah salah satu kabupaten di Sulawesi Selatan yang terkenal akan kelimpahan kekayaan hayati seperti kacang tanah, kakao, dan kelapa. Ketiga komoditas ini bila diolah melalui tahap fermentasi maka dapat menjadi bioproduk pangan yang berpotensi tinggi dan berkualitas. Fermentasi adalah proses tranformasi kimia substrat organik dengan memanfaatkan aktivitas enzim dari mikroorganisme. Kacang kedelai dan kacang tanah yang difermentasi serta penambahan ragi Rhizopus sp. menghasilkan bioproduk pangan yang dikenal sebagai tempe kalangka yang bermanfaat mencegah penyakit kanker payudara dan jantung koroner. Larutan palape dari limbah air kelapa dan kakao menjadi media asam yang mempercepat pertumbuhan ragi tempe. Nata de coko tinggi kandungan serat dapat meningkatkan kesehatan pencernaan. Metode pembuatan produk tersebut mudah untuk diterapkan. Kegiatan ini bertujuan memberikan pengetahuan akan cara pembuatan tempe, larutan palape dan nata de coko skala home industry, serta menumbuhkan kesadaran berwirausaha yang dapat memperbaiki taraf ekonomi keluarga di Kelurahan Tiroang, Kabupaten Pinrang. Langkah awal yang dilakukan yaitu mensurvei lokasi dan pembuatan ketiga produk dalam skala laboratorium. Pelaksanaan kegiatan dimulai dengan sesi penyuluhan materi manfaat ketiga produk beserta cara pembuatan desain pengemasan yang baik, dan diakhiri dengan sesi diskusi. Kegiatan ini dihadiri oleh target PKM, yaitu kaum ibu rumah tangga serta perwakilan daerah setempat. Kegiatan berlangsung dengan lancar dimana para hadirin mengikuti dengan seksama dan penuh antusias baik dalam sesi diskusi dan praktik. Survei data kegiatan melalui uji pre-test dan post test yang menunjukkan peningkatan pemahaman peserta terkait pelatihan yang diberikan. Diharapkan, kegiatan ini meningkatkan pemanfaatan komoditi lokal secara optimal.
A Review of Marine Bacterial Intracellular and Extracellular Bioactive Compounds as Novel Antibacterial and Anti-Inflammation Agents Karim, Harningsih; Azis, Arief; Ramadani, Ananda; Anita, Anita; Ahmad, Ahyar; Natsir, Hasnah; Taba, Paulina; Putri, Suriati Eka; Sarlan, Sarlan; Larekeng, Siti Halimah; Irfandi, Rizal
Indonesian Journal of Chemistry Vol 25, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.90039

Abstract

Unique and varied bioactive compounds produced by the ocean have drawn attention and served as a focus for creating antibacterial and anti-inflammatory agents. As part of the approach for locating these research sources, databases such as PubMed, Science Direct, MDPI, Google Scholar, Springer Link, Web of Science, Scopus, and Wiley Online Library were used to identify completed studies. Numerous intriguing bioactive compounds have so far been isolated from marine bacteria. A crucial resource in the ongoing search for novel peptides, proteins, lipids, nucleosides, enzymes, alkaloids, polyketides, and terpenoids is the diversity of marine bacterium strains. This review summarizes several bacterial intracellular and extracellular bioactive compounds that have been applied as antibacterial and anti-inflammatory agents in 2016–2024, which we present in the form of structures, species sources, and evaluations of these compounds' antibacterial and anti-inflammatory activities. Therefore, this review article can be used as a recommendation for utilizing biomaterials from marine bacteria that are promising in the future for industrial-scale production of antibacterial and anti-inflammatory agents.
Optimasi Pertumbuhan Bakteri Laut Penghasil Protease Menggunakan Variasi Konsentrasi Media Skim Milk Modifikasi Anita, Anita; Natsir, Hasnah; Ahmad, Ahyar; Taba, Paulina; Karim, Harningsih
Indobiosains 2025: Volume 7 Nomor 1 Februari 2025
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/indobiosains.v7i1.17822

Abstract

This study aims to analyze the growth rate of marine bacteria producing protease using variations in the concentration of skim milk media and to observe the relationship between cell growth and enzyme activity. The study was conducted using three concentrations of skim milk media (1%, 2%, and 3%) and observation of growth and enzyme activity for 108 hours. The results showed that 1% skim milk concentration produced the largest proteolytic zone diameter (13.8 mm) with the fastest growth (1 day). Observations of cell growth patterns and enzyme activity showed that the highest enzyme activity (0.5933 U/mL) occurred in the early growth phase and decreased in subsequent phases even though cell growth increased. This indicates that there is no linear relationship between cell growth and enzyme activity in marine bacteria-producing protease, where enzyme production and activity are influenced by various complex factors such as physiological conditions of the cells and nutrient availability.
PELATIHAN PENGOLAHAN AIR KELAPA MENJADI KECAP DI DESA MATTIRODECENG, KECAMATAN TIROANG, KABUPATEN PINRANG Natsir, Hasnah; Arif, Abdur Rahman; Arfah, Rugaiyah A; Zakir, Muhammad; St Fauziah, St Fauziah; Budi, Prastawa
Jurnal Dinamika Pengabdian Vol. 6 No. 1 (2020): JURNAL DINAMIKA PENGABDIAN VOL. 6 NO. 1 OKTOBER 2020
Publisher : Departemen Budidaya Pertanian Fakultas Pertanian UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jdp.v6i1.10009

Abstract

Mattirodeceng is a village located in Pinrang Regency, which has considerable natural resource potential in coconut production. But the utilization of coconut plants, especially the water of coconut, is still low. Coconut water only used for consumption purposes at certain times. The purpose of this activity is to provide training on the benefits and ways of processing coconut water into soy sauce for PKK groups in the Mattirodeceng village. The method used in this activity consisted of the pre-activity stage, which included a survey of the location and trial making of soy sauce made from coconut water. The implementation of activities carried out by counseling methods and training on the benefits and ways of processing coconut water into soy sauce. The result of the application of this activity was an increase in participants' knowledge about coconut water, namely its nutritional content, and its processed products in the form of soy sauce. The Organoleptic test showed the level of panelists' preference for taste, flavour, texture, and color showed good results, where all panelists liked the coconut water soy sauce products   Keywords: Coconut water, soy sauce, organoleptic Mattirodeceng Village.   ABSTRAK Mattirodeceng merupakan salah satu desa yang terletak di Kabupaten Pinrang yang memiliki potensi sumber daya alam yang cukup besar dalam produksi kelapa. Namun pemanfaatan tanaman kelapa, terutama air kelapa masih rendah. Air kelapa hanya digunakan untuk keperluan konsumsi pada saat-saat tertentu. Tujuan dari kegiatan ini yaitu untuk memberikan pelatihan manfaat dan cara pengolahan air kelapa menjadi kecap kepada kelompok PKK di desa mattirodeceng. Metode yang digunakan dalam kegiatan ini terdiri dari tahap pra kegiatan yang meliputi survei lokasi dan uji coba pembuatan kecap air kelapa. Tahap pelaksanaan kegiatan dengan metode penyuluhan dan pelatihan tentang manfaat serta cara pengolahan air kelapa menjadi kecap. Hasil dari pelaksanaan kegiatan ini adalah meningkatnya pengetahuan peserta tentang air kelapa, yaitu kandungan gizinya, dan produk olahannya berupa kecap. Hasil uji organoleptik menunjukkan tingkat kesukaan panelis terhadap rasa, aroma, tekstur dan warna menunjukkan hasil yang baik, dimana seluruh panelis menyukai produk kecap air kelapa.   Kata kunci: Air kelapa, kecap, organoleptik, Desa Mattirodeceng.
Co-Authors A Arfah, Rugaiyah Abdul Karim Abdur Rahman Arif, Abdur Rahman Ahyar Ahmad Alam, Muh. Nur Ali Muhakim Andi Evi Erviani Anggriani, Dhea Faraditha Anita Anita Arfah, Rugaiyah Arfah, Rugaiyah A Arfah, Rugaiyah A. Aziz, Fitriani Djabal Nur Basir dwyana, Zarsawaty Fauziah, St Gita, Gita Harningsih Karim Husnul Khotimah Indah Raya Irfandi, Rizal Karimah, Aulia Khairunnur, Siti Mardianti, Riska Mardiyanti, Riska Maswati Baharuddin Muh. Yusri Karim Muhammad Nadir Muhammad Zakir Munandar, Nurharis Musa, Bulkis Nur Asmi Nur Haedar Paulina Taba Permatasari, Nur Umriani Prastawa Budi Ramadani, Ananda Rasyid, Herlina Rauf, Wahyudin Riskawati Riskawati, Riskawati Sarlan, Sarlan Sarni Sarni Sartika Sartika Seniwati Dali SITI HALIMAH LAREKENG, SITI HALIMAH Situmorang, Sarah Victoria Octovianni Solly Aryza St Fauziah, St Fauziah Sukmawaty, Eka Suriati Eka Putri Syaharuddin Kasim Yunita, Vivi Alfi Yusafir Hala Zakir, Masculine