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All Journal Indonesian Green Technology Journal INDONESIAN JOURNAL OF ESSENTIAL OIL Jurnal Ilmu Kefarmasian Indonesia
Elvina Dhiaul Iftitah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Engineering Health Professions Medicine & Pharmacology Public Health Other

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Pengaruh Waktu Inkubasi terhadap Biotransformasi Minyak Jarak (Ricinus communis L.) oleh Aspergillus oryzae Dian Mulyani; Elvina Dhiaul Iftitah; Arie Srihardyastutie
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 3, No 2 (2018)
Publisher : Institut Atsiri Universitas Brawijaya

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Abstract

Biotransformasi mikrobial menggunakan jamur Aspergillus oryzae dilakukan dengan menggunakan orbital shaker pada temperatur  26oC, 130 rpm dan variasi waktu inkubasi 3, 5, 7 dan 9 hari. Produk yang dihasilkan dianalisis menggunakan GC-MS. Analisis difokuskan pada produk-produk senyawa intermediet, yang mengarah ke pembentukan senyawa lakton. Produk utama yang terbentuk melalui biotransformasi minyak jarak oleh Aspergillus oryzae  (26oC, 130 rpm) adalah etil heksadekanoat (8,24%, 3 hari), etil pentadekanoat (1,22%, 5 hari), etil pentadekanoat (56,28%, 7 hari) dan etil pentadekanoat (0,34%, 9 hari). Semakin lama waktu inkubasi diperoleh semakin banyak produk etil pentadekanoat yang terbentuk, dari 1,22% (5 hari) menjadi 56,28% (7 hari). Namun, mengalami penurunan menjadi 0,34% (9 hari). Senyawa etil pentadekanoat terbentuk maksimum pada hari ke-7. 
Perbandingan Tingkat Keefektifan Sintesis Hidroksisitronelal melalui Hidrasi Garam Natrium Sitronelil Bisulfit dan Sitronelal Reiza Tri Suciani Putri; Edi Priyo Utomo; Elvina Dhiaul Iftitah
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 2, No 2 (2017)
Publisher : Institut Atsiri Universitas Brawijaya

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Sintesis hidroksisitronelal dari minyak sereh wangi, diperoleh dengan menggunakan bahan dasar sitronelal dan garam natrium sitronelil bisulfit. Sitronelal dapat diisolasi dari minyak sereh wangi melalui reaksi penggaraman menggunakan NaHSO3 , yang diikuti reaksi hidrolisis dengan NaOH 10%. Hasil isolasi sitronelal dikarakterisasi dengan FTIR shimadzu dan Kromatografi Gas-Spektrometer Massa (KG-SM). Pada penelitian ini dilakukan reaksi hidrasi untuk masg-masing bahan dasar pada ikatan rangkap tak jenuh menggunakan katalis asam, yaitu HCl 1%, H2SO4 1%, dan H3PO4 1% selama 8 jam. Produk reaksi tersebut dikarakterisasi menggunakan KG-SM sehingga diperoleh % randemen masing-masing bahan dasar dan katalis asam. Hidroksisitronelal dengan bahan dasar garam natrium sitronelil bisulfit menghasilkan % randemen tertinggi 7,7 %, sedangkan % randemen tertinggi untuk bahan dasar sitronelal sebesar 26,9 %. Kedua % randemen tersebut diperoleh dari hasi reaksi hidrasi dengan katalis H3PO4 1%.
Prototipe Hand Sanitizer Nanoemulsi Berbasis Surfaktan Alami Lerak (Sapindus rarak) Sebagai Antibakteri Dinisa Eka Putri; Edi Priyo Utomo; Elvina Dhiaul Iftitah
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 2, No 2 (2017)
Publisher : Institut Atsiri Universitas Brawijaya

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Abstract

Telah dilakukan optimasi komposisi bahan dalam pembuatan nanoemulsi Hand Sanitazer dengan menggunakan pendekatan metoda permukaan respons. Bahan-bahan tersebut terdiri dari ekstrak lerak yang mempunyai aktivitas antibakteri Staphylococcus aureus, etanol dan Tween 80. Ketiga bahan tersebut diformulasikan menurut metoda permukaan respons Box-Behken sehingga dihasilkan 17 formula yang kemudian diuji karakternya meliputi ukuran nanoemulsi, kejernihan larutan, kestabilan larutan, kelngketan dan kecepatan kering. Pembentukan nanoemulsi dengan surfaktan Lerak pada prototipe hand sanitizer dengan dibantu proses sonikasi selama 2 jam dapat menghasilkan distribusi nanoemulsi berukuran 296 - 1000 nm sebanyak 1%. Optimasi hasil prototipe nanoemulsi hand sanitizer yang dilakukan menggunakan Design Expert 6.0.8 Portable menghasilkan komposisi optimum etanol 86,18%, ekstrak Lerak 2% dan tween 80 0,21%.  Pada komposisi tersebut menghasilkan titik optimal yang diprediksi memiliki ukuran nanoemulsi sebesar 667 nm dengan tingkat kejernihan  60,60%, stabilitas nanoemulsi lebih besar 5 jam 61,48%. Respon panellist terhadap  kelengketan sebesar 52,42% (tidak lengket) dan kecepatan kering kurang dari 1 menit sebesar 89,88%
Studi Pengaruh pH dan Konsentrasi Garam Prekursor AgNO3 dari Sintesis Nanokomposit ZnO-Ag Menggunakan Minyak Cengkeh (Syzygium aromaticum L.) serta Uji Aktivitas Antibakteri terhadap Escherichia coli Yuli Ainul Rosita; Elvina Dhiaul Iftitah; Masruroh Masruroh
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 3, No 2 (2018)
Publisher : Institut Atsiri Universitas Brawijaya

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Abstract

Penelitian ini mempelajari pengaruh pH dan konsentrasi garam prekursor AgNO3 dalam sintesis nanokomposit ZnO-Ag menggunakan bioreduktor minyak cengkeh (Syzygium aromaticum L.) dengan bantuan microwave. Variasi pH larutan yang digunakan adalah 6,9 dan 12. Kosentrasi garam prekursor AgNO3 dengan perbandingan [Zn(Ac)2.2H2O]:[AgNO3] adalah 5:1, 5:3 dan 5:5 mM. Karakterisasi hasil produk sintesis dianalisis dengan XRD dan SEM-EDS. Uji aktivitas antibakteri terhadap Escherichia coli menggunakan metode difusi sumuran. Hasil karakterisasi XRD menunjukkan bahwa pH dan konsentrasi garam prekursor AgNO3 mempengaruhi ukuran kristal. Semakin besar pH, maka ukuran kristal semakin besar. Semakin kecil konsentrasi garam prekursor AgNO3, maka semakin kecil ukuran kristal yaitu sekitar (8,31-41,23 nm). Hasil terbaik dengan ukuran terkecil pada pH 9 dan konsentrasi garam prekursor AgNO3 5:1 mM menunjukkan terbentuknya masing-masing puncak kristal ZnO, Zn(OH)2 dan Ag. Berdasarkan analisis SEM-EDS menunjukkan morfologi logam Ag yang tersebar pada logam ZnO dengan massa% Zn (51,73%), O (39,74%) dan Ag (8,53%). Hasil uji aktibakteri terhadap Escherichia coli mampu menghambat sebesar 16,5 mm.
Studi Sintesis Patchouli Asetat melalui Pembentukan Alkoksida dari Patchouli Alkohol Fidela Azaria Antasari; Elvina Dhiaul Iftitah; Edi Priyo Utomo
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 2, No 2 (2017)
Publisher : Institut Atsiri Universitas Brawijaya

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Abstract

Sintesis patchouli asetat telah dilakukan melalui pembentukan alkoksida dari patchouli alkohol. Patchouli alkoksida diperoleh dari mereaksikan patchouli alkohol dengan logam natrium. Reaksi asetilasi patchouli alkoksida dilakukan dengan menggunakan pereaksi asam asetat  glasial dengan  katalis H2SO4  yang dibandingkan dengan menggunakan pereaksi anhidrida asetat . Asam asetat glasial maupun anhidrida asetat digunakan sebagai sumber asetil yang bereaksi dengan ion alkoksida dari patchouli alkohol untuk menghasilkan produk ester yaitu patchouli asetat. Hasil sintesis yang diperoleh dikarakterisasi dengan menggunakan Kromatografi gas – spektrometer massa  dan didukung oleh spektrofotometer FT-IR serta analisis aroma berdasarkan uji organoleptik. Hasil analisis dengan KG-SM dan FT-IR menunjukkan sintesis dengan pereaksi anhidrida asetat lebih muda daripada asam asetat glasial meskipun diawali dengan pembentukan patchouli alkoksida dengan % rendemen sebesar 67,16 %. Senyawa patchouli asetat hasil sintesis memberikan aroma soft woody dengan latar sweet.
Isolasi dan Identifikasi Snyawa Geraniol dari Minyak Atsiri Tanaman Sereh Wangi Cymbopogon nardus (L.) Rendle Yelfi Anwar; Shirly Kumala; Elvina Dhiaul Iftitah; Partomuan Simanjuntak
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.403 KB) | DOI: 10.35814/jifi.v17i2.746

Abstract

Background: Indonesia is a major producer of several essential oils such as citronella oil, clove oil, cananga oil, vetiver oil, sandalwood oil, and patchouli oil. The Cymbopogon (Poaceae) genus has the most important components of essential oils, namely citral, geraniol, citronellol, citronellal. Geraniol is one of the most important chemical compounds in the aroma and fragrance industry and shows the nature of insecticides and natural insect repellents which show low toxicity and is suggested to represent a new class of chemoprevention agents for cancer, antimicrobial, anti-oxidant, anti-inflammatory and some vascular effects Transdermal drugs also attract the attention of researchers and scientists in the field of formulation. Objective: This study aimed to obtain geraniol compounds obtained from Cymbopogon nardus (L.) Rendle and identification. Methodology: The citronella, essential oil was obtained from the Cymbopogon nardus (L) Rendle plant using a water-steam distillation method, then fractionation was carried out and continued by purification isolation by column chromatography. Results: Based on the interpretation of IR data, Core magnetic resonance (proton and carbon RMI) and mass spectra for isolate 5 were determined as geraniol compounds. Conclusion: Geraniol can be obtained from Cymbopogon nardus (L) Rendle plants by water-steam distillation, fractionation and isolation methods.
Formulation and Physicochemical Properties of Kaffir Lime Oil-in-Water Beverage Emulsions and Antibacterial Test Vincentius Johar Windrayan Pambudi; Elvina Dhiaul Iftitah; Diah Mardiana
The Indonesian Green Technology Journal Vol 11, No 1 (2022)
Publisher : Program Pascasarjana Universitas Brawijaya

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Abstract

Abstract Essential oil inside beverage emulsion became an interesting subject to get a new value for the fast-moving customer goods (FMCG) market. In this study, kaffir lime oil (KFO) antibacterial test on formulation and physicochemical properties of kaffir lime oil in water beverage emulsions was investigated. The main ingredients of beverage emulsions are water, high fructose corn syrup (HFCS), Arabic gum as an emulsifier, vitamin E, and kaffir lime oil as flavoring and antibacterial. KFO composition used instrument with Stability of oil-in-water emulsion was observed by measuring physicochemical properties: turbidity, alkalinity, and conductivity. GC-MS characterization on kaffir lime essential oils revealed the top three components, i.e., citronella (46,47%), citronellol (12,22%), and citronellyl acetate (6,48%). FT-IR KFO spectrum had absorbance at 1726 (C=O stretching), 2922, and 2874 cm-1 (C-H stretching from aldehyde). Absorbance was also present on 1454 and 1379 cm-1 (C=C stretching) wavelengths, resembling citronella. Gum arabic with R−S(=O)₂−OH  group addition reduced pH value to 1.1 on each composition addition and increased conductivity by 317.3 μS/cm. Fructose addition reduced the value by 153.4 μS/cm. Turbidity value increased averagely by 46,9 NTU, then reduced by 14,4 NTU. Citronellal in KFO could hinder e-coli bacterial growth and had an alt value >72 CFU/mL. This formulation study produces a beverage product with pH, conductivity, and turbidity values following the standard and has criteria under the microbiological contamination limit of BPOM and SNI standards. Keywords: emulsified beverage, kaffir lime oil, physicochemical properties, stability, perception
The Activity of Lipase From Jatropha Seed (Jatropha Curcas L.) And Its Application On Hydrolysis of Castor Oil In Organic Solvent Taritsu Hazal Faradis; Arie Srihardyastutie; Elvina Dhiaul Iftitah
The Indonesian Green Technology Journal Vol. 11 No. 2 (2022)
Publisher : Postgraduate School Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.igtj.2022.011.02.02

Abstract

Jatropha curcas L. is one of the seed plants that have lipase activity and can work well in hydrolysis reactions and chemical synthesis. This study aims to determine lipase activity that reacts with castor oil in organic solvents. In this research, organic solvents, especially hydrophobic solvents, were used in the hydrolysis reaction to increase lipase's catalytic activity. The organic solvent used has a hydrophobicity level between 2 and 4, namely hexane. The research stages consisted of lipase isolation, lipase assay, the effect of adding metal ions assay, and analysis of the compound of castor oil. The results showed that jatropha seeds had lipase activity in crude of 0.603 U/mL and increased to 0.911 U/mL after the addition of K+. The result of hydrolysis and transesterification of castor oil by lipase is ricinoleic acid (4,58%) and methyl ricinoleic (11,67%), and the concentration of ricinoleic acid (17.09%) and methyl ricinoleic (60.83%) were increased after addition of K+. The esterification reaction produces alkyl ester compounds such as methyl palmitate, ethyl pentadecanoate, methyl linoleate, methyl oleate, and methyl stearate. The lipase isolated from jatropha seeds not only catalyzes the hydrolysis reaction but also catalyzes chemical synthesis reactions such as esterification and transesterification. Keywords: lipase enzyme, Jatropha seeds, isolation, biocatalyst, hexane.
Physicochemical Properties and Antibacterial Activity of Kaffir Lime Oil Emulsion Applicable to Baverage Product Vincentius Johar Windrayan Pambudi; Elvina Dhiaul Iftitah; Diah Mardiana
The Indonesian Green Technology Journal Vol. 11 No. 1 (2022)
Publisher : Postgraduate School Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.igtj.2022.011.01.02

Abstract

Essential oil inside beverage emulsion became an interesting subject to get a new value for the fast-moving customer goods (FMCG) market. In this study, kaffir lime oil (KFO) antibacterial test on formulation and physicochemical properties of kaffir lime oil in water beverage emulsions was investigated. The main ingredients of beverage emulsions are water, high fructose corn syrup (HFCS), Arabic gum as an emulsifier, vitamin E, and kaffir lime oil as flavoring and antibacterial. KFO composition used instrument with Stability of oil-in-water emulsion was observed by measuring physicochemical properties: turbidity, alkalinity, and conductivity. GC-MS characterization on kaffir lime essential oils revealed the top three components, i.e., citronella (46,47%), citronellol (12,22%), and citronellyl acetate (6,48%). FT-IR KFO spectrum had absorbance at 1726 (C=O stretching), 2922, and 2874 cm-1 (C-H stretching from aldehyde). Absorbance was also present on 1454 and 1379 cm-1 (C=C stretching) wavelengths, resembling citronella. Gum arabic with R−S(=O)₂−OH  group addition reduced pH value to 1.1 on each composition addition and increased conductivity by 317.3 μS/cm. Fructose addition reduced the value by 153.4 μS/cm. Turbidity value increased averagely by 46,9 NTU, then reduced by 14,4 NTU. Citronellal in KFO could hinder e-coli bacterial growth and had an alt value >72 CFU/mL. This formulation study produces a beverage product with pH, conductivity, and turbidity values following the standard and has criteria under the microbiological contamination limit of BPOM and SNI standards. Keywords: emulsified beverage, kaffir lime oil, physicochemical properties, stability, perception
Antibacterial Retention of Star Anise (Illicium verum) Essential Oil and Oleoresin in the Cellulose-PEG Composite Miranda, Fitri Ariadna Sodi; Elvina Dhiaul Iftitah; Warsito
Indonesian Green Technology Journal Vol. 12 No. 1 (2023)
Publisher : Sekolah Pascasarjana, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.igtj.2023.012.01.01

Abstract

Star anise essential oil (EO) and oleoresin (OL) consists of various volatile compounds that can be easily damaged and affect their quality and function. The degradation is mainly caused by the deterioration and release of their active components. Encapsulating the extracts into a composite matrix can give slow release and minimize the contact between active compounds and surrounding. Cellulose-PEG composite has good compatibility and affinity, it can retain the extracts so does their biological activities. In this work, the essential oil and oleoresin were encapsulated into the cellulose-PEG composite with a ratio of 1:2 (µL EO and OL per mg of composite). The cellulose was obtained from extraction of star anise residue of EO and OL isolation. The extraction steps were pre-alkalization, alkalization, and three-stage bleaching. Antibacterial assay for the samples uses a combination of agar disc and well method, where the pure one uses the disc as absorbent and both the disc and composite are put in the well. The diameter of the inhibition zone against Staphylococcus aureus was observed once a week for eight week. The result shows that pure and composite-packed star anise essential oil and oleoresin samples have fluctuating antibacterial activity during the obeservation. Both packed EO and OL show a lower difference of the diameter of the inhibiton zone which indicated the composite can retain the antibacterial activity of the star anise essential oil and oleoresin. Keywords: Star anise, antibacterial, essential oil, oleoresin, composite.
Co-Authors Anwar, Yelfi Arie Srihardyastutie Diah Mardiana Dian Mulyani Dinisa Eka Putri Edi Priyo Utomo Fidela Azaria Antasari Masruroh Masruroh Miranda, Fitri Ariadna Sodi Partomuan Simanjuntak Reiza Tri Suciani Putri Shirly Kumala Taritsu Hazal Faradis Vincentius Johar Windrayan Pambudi Warsito Yuli Ainul Rosita