Joko Prayitno
Institute for Environmental Technology, Badan Penerapan dan Pengkajian Teknologi, Puspiptek Serpong, Tangerang 15314

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Journal : Jurnal Ecolab

DEGRADASI MINYAK MENTAH DAN SOLAR OLEH KONSORSIUM MIKROBA ASAL PERTAMBANGAN MINYAK CEPU Joko Prayitno; Esi Liyastuti; Amalia Mahmudah
Jurnal Ecolab Vol 4, No 2 (2010): Jurnal Ecolab
Publisher : Pusat Standardisasi Instrumen Kualitas Lingkungan Hidup Laboratorium Lingkungan (P3KLL)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jklh.2010.4.2.81-88

Abstract

The aim of this research was to obtain oil-degrading microbes from Cepu mining area and to investigate the best combination of microbial consortium to degrade crude and diesel oil. Two yeast isolates were obtained out of ten isolates from Cepu samples. Four best isolates (C7, C6, C4 an C3) were then selected and used for consortium experiments. All consortia either grown in crude or diesel oil showed increased in absorbance values (as the indication of oil degradation) and increased in cell populations at day three. However, pH of the medium decreased from 6.5 at day zero to 2.9-3.5 at day three. After day three, the absorbance values, cell populations and pHs tend to flat or slightly reduced. The best combination of microbial consortium was K3, containing C7, C6 and C4 isolates. This consortium was able to degrade 49-50% of crude or diesel oil for 12 day.