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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Kebun Raya
Eka Martha Della Rahayu
Pusat Konservasi Tumbuhan Kebun Raya Bogor - LIPI, Jl. Ir. H. Juanda No. 13, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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UPAYA KONSERVASI EX SITU DIPTEROCARPACEAE DI KEBUN RAYA BOGOR Rahayu, Eka Martha Della
Buletin Kebun Raya Vol 12, No 2 (2009): Buletin Kebun Raya Vol. 12 (2) Juli 2009
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Malesia is the center of Dipterocarps diversity in the world, with most species found in Indonesia. Sadly, their existence is threatened by illegal logging, extensive exploitation, and habitat conversion. There are 143 Indonesian Dipterocarps categorized as threatened in the IUCN Red List. Bogor Botanic Garden (BBG) is one of the leading institutions focuses on ex situ conservation of plants. Dipterocarps collection in the BBG represents 41.5% of Dipterocarps of Sumatra, 17.0% of Dipterocarps of Kalimantan, 77.8% of Dipterocarps of Java, 66.7% of Dipterocarps of Bali and Nusa Tenggara, 57.1% of Dipterocarps of Sulawesi, 66.7% Dipterocarps of Moluccas, and 8.3% Dipterocarps of Papua. Totally, BBG has only conserved about 37.8% of threatened Indonesian Dipterocarps, still far from the target aimed by the Global Strategy for Plant Conservation (60% conserved). The BBG has initiated genetic resource conservation of Dipterocarps, through seed preservation. Since most Dipterocarps have recalcitrant seeds, conventional storage methods cannot be used. One of the alternative solutions is to cryopreserve the excised embryo or embryonic axes to overcome the limits of conventional storage methods. Collection of threatened and endemic species along with research on embryo culture and cryopreservation should be intensified in the future.
UPAYA KONSERVASI EX SITU DIPTEROCARPACEAE DI KEBUN RAYA BOGOR Rahayu, Eka Martha Della
Buletin Kebun Raya Vol 12, No 2 (2009): Buletin Kebun Raya Indonesia Vol. 12 (2) July 2009
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Malesia is the center of Dipterocarps diversity in the world, with most species found in Indonesia. Sadly, their existence is threatened by illegal logging, extensive exploitation, and habitat conversion. There are 143 Indonesian Dipterocarps categorized as threatened in the IUCN Red List. Bogor Botanic Garden (BBG) is one of the leading institutions focuses on ex situ conservation of plants. Dipterocarps collection in the BBG represents 41.5% of Dipterocarps of Sumatra, 17.0% of Dipterocarps of Kalimantan, 77.8% of Dipterocarps of Java, 66.7% of Dipterocarps of Bali and Nusa Tenggara, 57.1% of Dipterocarps of Sulawesi, 66.7% Dipterocarps of Moluccas, and 8.3% Dipterocarps of Papua. Totally, BBG has only conserved about 37.8% of threatened Indonesian Dipterocarps, still far from the target aimed by the Global Strategy for Plant Conservation (60% conserved). The BBG has initiated genetic resource conservation of Dipterocarps, through seed preservation. Since most Dipterocarps have recalcitrant seeds, conventional storage methods cannot be used. One of the alternative solutions is to cryopreserve the excised embryo or embryonic axes to overcome the limits of conventional storage methods. Collection of threatened and endemic species along with research on embryo culture and cryopreservation should be intensified in the future.
INDUKSI POLIPLOIDI MENGGUNAKAN KOLKISIN SECARA IN VIVO PADA BIBIT ANGGREK BULAN (Phalaenopsis amabilis (L.) BLUME) Rahayu, Eka Martha Della; Sukma, Dewi; Syukur, M.; Aziz, Sandra A.; Irawati, Irawati
Buletin Kebun Raya Vol 18, No 1 (2015): Buletin Kebun Raya Vol. 18 (1) January 2015
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Induksi poliploidi pada bibit Phalaenopsis amabilis telah dilakukan menggunakan kolkisin secara in vivo. Induksi poliploidi dilakukan dengan meneteskan kolkisin pada pucuk bibit P. amabilis.  Tujuan penelitian ini adalah untuk mendapatkan konsentrasi kolkisin yang efektif untuk induksi poliploidi bibit P. amabilis dan  menghasilkan bibit P. amabilis poliploid. Percobaan disusun dalam rancangan kelompok lengkap teracak dengan satu faktor, yaitu konsentrasi kolkisin. Pucuk bibit P. amabilis ditetesi 0,01 ml kolkisin (0, 1000, 2000, 3000, 4000, dan 5000 mg L-1). Hasil percobaan menunjukkan bahwa peningkatan konsentrasi kolkisin dari 1000 sampai 5000 mg L-1 tidak berpengaruh nyata terhadap persentase hidup dan pertumbuhan bibit pada 24 minggu setelah perlakuan (24 MSP). Bibit P. amabilis poliploid dapat dihasilkan pada penetesan kolkisin 1000, 3000, 4000, dan 5000 mg L-1 dengan konsentrasi kolkisin paling efektif adalah 5000 mg L-1. Bibit poliploid memiliki ukuran stomata lebih besar dari bibit diploid sebaliknya kerapatan stomatanya lebih rendah.
Induksi Poliploidi Phalaenopsis amabilis (L.) Blume dan Phalaenopsis amboinensis J. J. Smith dengan Kolkisin dalam Kultur In Vitro Rahayu, Eka Martha Della; Sukma, Dewi; Syukur, Muhamad; ,, rawati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 3 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.73 KB) | DOI: 10.24831/jai.v43i3.11248

Abstract

ABSTRACTPhalaenopsis amabilis (L.) Blume and Phalaenopsis amboinensis J.J. Smith (diploid) are important in Phalaenopsis breeding. Polyploid species are needed for crossing with polyploid hybrid varities of Phalaenopsis. The objectives of this study were to obtain effective concentration of colchicine to induce polyploidy and to produce polyploid plantlets of P. amabilis and P. amboinensis. Experiment was arranged in randomized complete block design with one factor, the colchicine concentration. Protocorms of P. amabilis and P. amboinensis were immersed in half strength of Murashige-Skoog (1/2 MS) liquid media added with colchicine (0; 0.5; 5; 25; 50, and 75 mg L-1) for 10 days. The results showed that higher concentration of colchicine on both species did not have significant effect on the survival of the plantlets at 24 weeks after treatment. The average number of leaves and roots of colchicine treated planlets from both species were less than the control plantlets. Immersing protocorm in colchicine at concentration of 50 mg L-1 for 10 days was effective in inducing polyploid plantlets of P. amabilis and P. amboinensis with the frequency of 33.3% and 40%, respectively. Polyploid plantlet has larger stomata size and lower stomata density than the diploid ones.Keywords: chromosome number, colchicine, polyploid, protocorm, stomatal density, stomatal size
Mikromorfologi dan Perkecambahan In Vitro Biji Anggrek Endemik Sulawesi: Phalaenopsis venosa Shim & Fowlie Rahayu, Eka Martha Della; Winda Utami Putri; Fitri Fatma Wardani; Lydia Natalia Endewip
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 1 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (654.242 KB) | DOI: 10.24831/jai.v50i1.39012

Abstract

Phalaenopsis venosa Shim & Fowlie adalah anggrek endemik Sulawesi terancam punah sehingga perlu dilakukan upaya konservasi. Tujuan penelitian untuk mengetahui mikromorfologi dan media perkecambahan in vitro untuk mendukung upaya konservasi P. venosa. Biji P. venosa dipanen pada enam bulan setelah penyerbukan dan disimpan di freezer pada suhu -4 °C selama sembilan bulan. Pengamatan mikromorfologi menggunakan mikroskop cahaya OPTIKA M-699. Biji P. venosa memiliki panjang 91.99±17.73 µm, lebar 20.64 ±4.34 µm; serta embrio dengan panjang 52.27±12.13 µm, lebar 16.72±3.42 µm, dan rongga udara 13.25% ± 11.65%. Pengujian perkecambahan menggunakan rancangan acak lengkap dengan satu faktor, yaitu enam macam media, dan enam ulangan. Media perkecambahan yang diujikan yaitu Hyponex modifikasi (HS), Vacin & Went modifikasi (mVW), Knudson’s C modifikasi (mKC), Knudson’s C (KCA), Murashige & Skoog setengah konsentrasi (1/2MS), dan Murashige & Skoog (MS). Tingkat perkecambahan tertinggi pada 12 minggu setelah tanam (12 MST) terdapat pada media HS (70.52%) dengan rata-rata 14.35 biji berkecambah. Perkembangan protokorm di media HS lebih lambat dibandingkan perkembangan protokorm di media 1/2MS yang menunjukkan pembentukan rhizoid dan pucuk daun. Hal itu karena media 1/2MS lebih kaya nutrisi dibandingkan dengan media HS. Kata kunci: media perkecambahan, mikromorfologi biji, Orchidaceae
Co-Authors Dewi Sukma Fitri Fatma Wardani Irawati Irawati Lydia Natalia Endewip M. Syukur Muhamad Syukur rawati ,, rawati Sandra A. Aziz Winda Utami Putri