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Khamir Penghasil Indole-3-Acetic Acid dari Rhizosfer Anggrek Tanah Pecteilis susannae (L.) Rafin Lasmini, Titi; Sutariningsih Soetarto, Endang
Biogenesis Vol 2, No 1 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Indole-3-acetic acid (IAA) is a growth hormone produced by plants. Several types of yeast has been reported to produce these hormones, and generally produce IAA in an higher amount than in plants. The purpose of this study is to get the yeast producing indole-3-acetic acid in the rhizosphere soil orchid Pecteilis susannae (L.) Rafin and to test the ability of yeast isolates to form IAA on medium L-tryptophan given as precursors. Yeasts were isolated and tested for their ability to produce IAA in vitro. The results showed that there are four potential yeast isolates that produce IAA which are isolates K2, U5, A3, and A1. Highest concentration of IAA was produced by A3 isolates grown on a medium containing GPB 200 ug / ml of L tryptophan in the amount of 56.262 g/ml.Keywords: Indole-3-acetic acid, rhizosfer yeast, Pecteilis susannae
Pengaruh Obat Kumur Berbasis Fluoride Dan Essential Oils Terhadap Jumlah Angka Kuman Rongga Mulut Lasmini, Titi
Jurnal Ilmiah Kesehatan Vol 14 No 1 (2022): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v14i1.475

Abstract

Mouthwash is a solution or liquid that is used to clean the oral cavity with the aim of eliminating the bacteria causing tooth decay, working as a refresher and eliminating bad breath. The purpose of this study were to determine the differences of bacterial colony count in the oral cavity before and after using fluoride and essential oils based mouthwash, and to compare the effectiveness of both mouthwashes in reducing the number of oral bacteria. The sample for this study was mouth rinse collected from 10 students of Akademi Kesehatan John Paul II Pekanbaru, before and after using mouthwash. The samples were inoculated on Nutrient agar by pour plate method, and the bacterial colonies (CFU/mL) were counted after 2 days incubation.The results of this study showed that there was a difference of colony count between before and after the used of both mouthwashes. The mean of bacterial colony count after using fluoride-based mouthwash decreased  by 314830.00 CFU/mL, while  essential oils-based mouthwash reduced the bacterial colony count by 880220.00 CFU/mL. Dependent T-test showed that there were statistically significant reduction of bacterial colony count after the used of fluoride-based mouthwash (P=0.034) and essential oils-based mouthwash (P=0.008). Based on the Independent T-test there was no statistically significant difference (P>0.05) between fluoride and essential oils based mouthwash in reducing oral bacterial count.
Edukasi Urgensi Pemeriksaan Golongan Darah dan Aplikasinya pada Santriwati Al-Fityan School Aceh Safwan; Hadijah, Siti; Darmawati; Erlinawati; Putri, Safridha Kemala; Fitriana; Fajarna, Farah; Syahnita, Hastuti; Situmorang, Paska Ramawati; Mutia, Liza; Lubis, Nita Andriani; Lasmini, Titi; Rosmiati, Karolina; Wardhani, Sri Muri Dasa
AJAD : Jurnal Pengabdian kepada Masyarakat Vol. 4 No. 3 (2024): DECEMBER 2024
Publisher : Divisi Riset, Lembaga Mitra Solusi Teknologi Informasi (L-MSTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59431/ajad.v4i3.390

Abstract

Blood type is important for human life and is very useful in medical matters such as determining the blood type of parents and children, the success of medical procedures such as blood transfusions and organ transplants. Transfusion of blood from an incompatible group causes an immunological transfusion reaction resulting in hemolytic anemia, kidney failure, shock and death. Most of the students of Al-Fityan School Aceh's tahfiz program do not know their blood type. Apart from that, the students of the Al-Fityan School Aceh tahfiz program have minimal knowledge about blood types. The aim of this community service is to increase students' knowledge about the urgency of checking blood types and its application in everyday life. The target of this community service is the female students of the Al-Fityan School Aceh tahfiz program, consisting of 47 people. The implementation begins with education first and continues with a blood type check. Examination Results: The blood group of the female students of Tahfis Al- Fityan School Aceh is blood group O, namely 70% (31 people), while blood groups A and B are 12.8% (6 people) respectively and the smallest blood group frequency is blood group AB, namely 8.5% (4 people).
Pengaruh Obat Kumur Berbasis Fluoride Dan Essential Oils Terhadap Jumlah Angka Kuman Rongga Mulut Lasmini, Titi
Jurnal Ilmiah Kesehatan Vol 14 No 1 (2022): Jurnal Ilmiah Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/jik.v14i1.475

Abstract

Mouthwash is a solution or liquid that is used to clean the oral cavity with the aim of eliminating the bacteria causing tooth decay, working as a refresher and eliminating bad breath. The purpose of this study were to determine the differences of bacterial colony count in the oral cavity before and after using fluoride and essential oils based mouthwash, and to compare the effectiveness of both mouthwashes in reducing the number of oral bacteria. The sample for this study was mouth rinse collected from 10 students of Akademi Kesehatan John Paul II Pekanbaru, before and after using mouthwash. The samples were inoculated on Nutrient agar by pour plate method, and the bacterial colonies (CFU/mL) were counted after 2 days incubation.The results of this study showed that there was a difference of colony count between before and after the used of both mouthwashes. The mean of bacterial colony count after using fluoride-based mouthwash decreased  by 314830.00 CFU/mL, while  essential oils-based mouthwash reduced the bacterial colony count by 880220.00 CFU/mL. Dependent T-test showed that there were statistically significant reduction of bacterial colony count after the used of fluoride-based mouthwash (P=0.034) and essential oils-based mouthwash (P=0.008). Based on the Independent T-test there was no statistically significant difference (P>0.05) between fluoride and essential oils based mouthwash in reducing oral bacterial count.
Isolation and Screening of Proteolytic Bacteria Isolated from the Digestive Tract of Pangasius Catfish Patin (Pangasius hypophthalmus) Zafrida, Siska; Lasmini, Titi; Hartini, Hartini; Aruan, Anggi Agustina
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

The demand for protease enzymes in Indonesia has exhibited a significant upward trend; however, domestic large-scale production capacity remains limited. To mitigate dependency on imports, strategic efforts to develop indigenous protease production are essential. This study aims to identify and isolate protease-producing bacteria from the digestive tract of catfish (Pangasius hypophthalmus). This research employed an observational method with a cross-sectional design. Samples were obtained from the stomach of the catfish and subsequently inoculated into Brain Heart Infusion (BHI) media. Bacterial isolation was performed using Blood Agar Plate (BAP) and MacConkey Agar. The proteolytic activity of the resulting isolates was evaluated on Skim Milk Agar (SMA), followed by macroscopic and microscopic characterization. The study yielded three pure isolates; however, screening for proteolytic activity revealed that only one isolate, designated as PHS1, exhibited protease production. The proteolytic activity was characterized by the formation of a 2 mm clear zone (lysis zone) with a proteolytic index of 3. Microscopic analysis identified the isolate as a Gram-positive, spore-forming bacterium.