Asri Pirade Paserang
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Tadulako

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PENGARUH PUPUK HAYATI DENGAN KOMPOSISI MIKROORGANISME YANG BERBEDA TERHADAP PERTUMBUHAN TANAMAN Amalia Puji` Cahyani; Asri Pirade Paserang; Meryany Ananda; Wahyu Harso
Biocelebes Vol. 15 No. 2 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i2.15781

Abstract

Biofertilizer can be used to replace chemical fertilizer so that soil quality is maintained and soil pollution can be avoided. The study aimed to determine the effect of biofertilizers with different compositions of microorganisms on the growth of maize plants. The research was carried out in a greenhouse. Maize plants were fertilized by two kinds of biofertilizers with different compositions of microorganisms. The first biofertilizer contained Azotobacter sp, Azospirillum sp, Rhizobium sp, Trichoderma sp, and Lactobacillus sp, and the second biofertilizer contained Strenotrophomonas sp. and Paenibacillus polymyxa. As controls, maize plants were fertilized by sterilized those biofertilizers. The results showed that the second biofertilizer increased the dry weight of the maize plant. Meanwhile, the first biofertilizer did not increase it. Several things must be considered in the application of biofertilizers, such as the composition of microorganisms, type of plant, level of fertilization, and the method of applying fertilizer to the plant.
PENGARUH KOMBINASI IAA (Indole-3-Acetic Acid) DAN BAP (6-Benzylaminopurine) TERHADAP INISIASI TANAMAN VANILI (Vanilla planifolia Andrews) Apri Salfiani; Asri Pirade Paserang
Biocelebes Vol. 15 No. 2 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i2.15782

Abstract

Provision of Vanilla (Vanilla planifolia Andrews) with traditional cultivation often has problems, including the availability of unhealthy seeds due to disease caused by Fusarium oxysporum. This disease can thwart vanilla plantations up to 85% because the pathogen can infect all parts of the vanilla plant making it difficult to control efforts. Alternative efforts were made to overcome this problem, namely through the initiation process in tissue culture by combining the hormones IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine). This study aims to determine the effect and the maximum concentration of the combination of IAA and BAP on the initiation of vanilla plants. This study was conducted based on a completely randomized design (CRD) with 5 treatments and 3 repetitions. This treatment consisted of: A (MS + 0.1 ppm IAA + 1 ppm BAP), B (MS + 0.2 ppm IAA + 1 ppm BAP), C (MS + 0.3 ppm IAA + 1 ppm BAP), D (MS + 0.4 ppm IAA + 1 ppm BAP), and E (MS + 0.5 ppm IAA + 1 ppm BAP). Observations were made after 40 days from the planting process. The results showed that the concentration of the combination of IAA and BAP can affect the growth and organogenesis of the initiation of vanilla plants (Vanilla planifolia Andrews). Treatment C (MS + 0.3 ppm IAA + 1 ppm BAP) was the best concentration from this study, with average values: number of shoots (1), shoot length (1.73 cm), number of roots (1), and number of leaves (1.33).
APLIKASI HORMON BAP, NAA, AIR KELAPA TERHADAP MULTIPLIKASI PISANG CAVENDISH (Musa acuminata L.) SECARA IN VITRO Asri Pirade Paserang; Riska Riska
Biocelebes Vol. 16 No. 1 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i1.15949

Abstract

This study aims to determine the effect of proper concentration of coconut water on the shoot multiplication of cavendish banana (M. acuminata L.) with the addition of the NAA and BAP hormones. This study used a Completely Randomized Design (CRD) with 8 treatments. Each treatment repeated 3 times. Therefore, there were 24 experimental units. Each unit using 1 explant so there were 24 explants. The treatments consisted of: A1 (0% coconut water + 0.2 ppm NAA), A2 (15% coconut water + 0.2 ppm NAA), A3 (20% coconut water + 0.2 ppm NAA), A4 (coconut water 25% + NAA 0.2 ppm), A5 (0% coconut water + BAP 2 ppm), A6 (15% coconut water + BAP 2 ppm), A7 (20% coconut water + 2 ppm BAP) and A8 (coconut water 25% + BAP 2 ppm). The parameters observed were days to the emergence of shoots, number of shoots and percentage of the number of shoots. The results showed that the addition of the 2 ppm BAP hormone which is in treatments A7 showed the fastest emergence of shoots with an average of 8 DAP and the average number of shoots that appeared was 2,67 and the percentage of the number of shoots was 88,89%.
Transformation of Inhibitor of Meristem Activity (IMA) Gene into Jatropha curcas L. Paserang, Asri Pirade; Tjahjoleksono, Aris; Widyastuti, Utut; Suharsono,
Makara Journal of Science Vol. 19, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Jatropha is one of the many biodiesel plants developed in tropical countries. Efforts to increase its productivity can be done using various methods of breeding. One of the breeding methods is the introduction of genes into the Jatropha plant. The aim of this study is to assess the success of genetic transformation using the Inhibitor of Meristem Activity (IMA) gene in Jatropha curcas. The research procedures included inoculation of explants with Agrobacterium tumefaciens, callus induction, screening test of selection media, regeneration, and gene expression analysis using Polymerase Chain Reaction (PCR). IMA is one of the genes that controls flowering genes and ovule development. It was first isolated from tomato plants and has been successfully overexpressed in these plants using the Cauliflower Mosaic Virus (CaMV) 35S promoter. In this experiment, plant transformation was performed on J. curcas as the target. Explant callus formation in both the control and treated samples was good, but shoot formation decreased dramatically in the treated explants. PCR analysis indicated that IMA genes can be inserted into J. curcas with the size of the IMA gene is 500 bp.
MELISSOPALYNOLOGICAL ANALYSIS OF HONEY FROM DOLAGO VILLAGE, CENTRAL SULAWESI Rasyiid, Mustafid; Iqbal, Moh.; Paserang, Asri Pirade; Budiono, Yudhistira Kristinov; Nugraha, Muh. Ardian Satrio; Wiguna, Triadhy
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 11 No. 2 (2025): Biolink February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v11i2.13402

Abstract

One thing that honeybees create that is very beneficial to human health is honey. Consequently, honey is frequently faked. With melissopalynology analysis, honey quality can be ascertained. A technique for analyzing pollen in honey to ascertain its botanical and geographic origin is called melissopalynology analysis.This study uses melissopalynology to analyze the quality and pollen composition of honey produced by Apis cerana bees in Dolago Village, Central Sulawesi. The research consists of five stages: collecting honey, extracting pollen from it using a modified acetolysis method, identifying pollen, classifying honey, and last, absolute pollen count. The analysis identified 11 plant species contributing to honey production, with Cocos nucifera as the predominant pollen (70%). Classified as unifloral honey with Group IV pollen richness, this research confirms the high quality and unique floral origin of Dolago honey, emphasizing its potential for local economic development.
PERTUMBUHAN TANAMAN DAN STRUKTUR ANATOMI DAUN KEDELAI, KACANG PANJANG, DAN KACANG TANAH PADA KETINGGIAN TEMPAT YANG BERBEDA Andre, Andre; Harso, Wahyu; Rasyiid, Mustafid; Paserang, Asri Pirade; Iqbal, Moh; Sari, Retno
Biocelebes Vol. 19 No. 1 (2025)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v19i1.17357

Abstract

Soybeans, yardlong beans and peanuts are cultivated crops widely grown in the tropics at different altitudes. Differences in environmental factors between lowlands and highlands will affect plant growth and leaf anatomical structure. The study aimed to determine the growth and anatomical structure of the leaves of soybean, yardlong bean and peanut plants at different altitudes. The test plants were grown at two different altitudes. At an altitude of ± 20 masl, the test plants were placed in the greenhouse of the Biology Department of the Faculty of Mathematics and Natural Sciences, Tadulako University and at an altitude of ± 630 masl, the test plants were placed in Bahagia Village, Palolo District, Sigi Regency. The results showed that yardlong bean and peanut plants grown at an altitude of ± 630 masl had a lower stomatal density than those grown at ± 20 masl so the plant dry weight produced was also lower. Soybean plants have a larger stem diameter and plant dry weight at an altitude of ± 630 masl, these are due to the higher stomatal density and leaf thickness compared to those grown at an altitude of ± 20 masl.