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Efek Ekstrak Daun Singawalang (Petiveria alliacea) dalam Menurunkan Kadar Glukosa Darah melalui Peningkatan Ekspresi AMPK-Î±1 pada Tikus Model Diabetes Melitus Mustika, Arifa; Indrawati, Roostantia; Sari, Gadis M.
Indonesian Journal of Clinical Pharmacy Vol 6, No 1 (2017)
Publisher : Indonesian Journal of Clinical Pharmacy

Singawalang (Petiveria alliaceae) adalah tumbuhan yang banyak ditemukan di Indonesia dan secaraempiris digunakan oleh penduduk untuk mengobati diabetes melitus. Hingga saat ini, penelitian tentangpengaruh dan mekanisme ekstrak daun Singawalang dalam menurunkan kadar glukosa darah belumbanyak dilakukan. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak daun Singawalangterhadap penurunan kadar glukosa darah dan ekspresi AMPK-Î±1 pada hati. Jenis penelitian ini adalahpenelitian eksperimental dengan rncangan acak lengkap. Penelitian dilakukan di LaboratoriumFarmakologi dan Terapi dan Laboratorium Patologi Anatomi Fakultas Kedokteran Universitas Airlanggapada bulan AprilâAgustus 2015. Hewan coba tikus strain Rattus norvegicus dibuat model diabetesmelitus, diinduksi dengan Streptozotocin. Sebanyak 25 tikus model diabetes melitus dibagi secara acakmenjadi 5 kelompok. Kelompok perlakuan 1, 2 dan 3 adalah kelompok yang memperoleh ekstrak daunSingawalang dengan dosis 90 mg/kgbb, 180 mg/kgbb dan 360 mg/kgbb. Kelompok kontrol positif adalahkelompok yang memperoleh metformin dengan dosis 150 mg/kgbb dan kontrol negatif adalah kelompoktikus yang memperoleh vehikulum. Ekstrak dan metformin diberikan sehari sekali, personde selama 14hari. Pada hari ke-15 setelah terapi, tikus diperiksa kadar glukosa darah dan dieuthanasia untuk diambilorgannya. Ekspresi AMPK-Î±1 dinilai menggunakan imunohistokima. Data yang terkumpul dianalisisdengan ANAVA dan Wilcoxon (Î±=0,05). Hasil penelitian menunjukkan adanya perbedaan kadar glukosadarah yang bermakna antara kontrol negatif dengan dosis 90 mg/kgbb, dosis 360 mg/kgbb, dan kontrolpositif, antara dosis 180 mg/kgbb dengan dosis 360 mg/kgbb dan kontrol positif. Analisis terhadapekspresi AMPK-Î±1 pada hati tikus menunjukkan perbedaan yang bermakna antara dosis 180 mg/kgbbdengan dosis 360 mg/kgbb dan kontrol positif. Simpulan dari penelitian ini adalah ekstrak Singawalangdapat menurunkan kadar glukosa darah pada tikus model diabetes melitus melalui aktivasi AMPK-Î±1.

Efek Ekstrak Daun Singawalang (Petiveria alliacea) dalam Menurunkan Kadar Glukosa Darah melalui Peningkatan Ekspresi AMPK-α1 pada Tikus Model Diabetes Melitus Arifa Mustika; Roostantia Indrawati; Gadis M. Sari
Indonesian Journal of Clinical Pharmacy Vol 6, No 1 (2017)
Publisher : Universitas Padjadjaran

Singawalang (Petiveria alliaceae) adalah tumbuhan yang banyak ditemukan di Indonesia dan secara empiris digunakan oleh penduduk untuk mengobati diabetes melitus. Hingga saat ini, penelitian tentang pengaruh dan mekanisme ekstrak daun Singawalang dalam menurunkan kadar glukosa darah belum banyak dilakukan. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak daun Singawalang terhadap penurunan kadar glukosa darah dan ekspresi AMPK-α1 pada hati. Jenis penelitian ini adalah penelitian eksperimental dengan rncangan acak lengkap. Penelitian dilakukan di Laboratorium Farmakologi dan Terapi dan Laboratorium Patologi Anatomi Fakultas Kedokteran Universitas Airlangga pada bulan April–Agustus 2015. Hewan coba tikus strain Rattus norvegicus dibuat model diabetes melitus, diinduksi dengan Streptozotocin. Sebanyak 25 tikus model diabetes melitus dibagi secara acak menjadi 5 kelompok. Kelompok perlakuan 1, 2 dan 3 adalah kelompok yang memperoleh ekstrak daun Singawalang dengan dosis 90 mg/kgbb, 180 mg/kgbb dan 360 mg/kgbb. Kelompok kontrol positif adalah kelompok yang memperoleh metformin dengan dosis 150 mg/kgbb dan kontrol negatif adalah kelompok tikus yang memperoleh vehikulum. Ekstrak dan metformin diberikan sehari sekali, personde selama 14 hari. Pada hari ke-15 setelah terapi, tikus diperiksa kadar glukosa darah dan dieuthanasia untuk diambil organnya. Ekspresi AMPK-α1 dinilai menggunakan imunohistokima. Data yang terkumpul dianalisis dengan ANAVA dan Wilcoxon (α=0,05). Hasil penelitian menunjukkan adanya perbedaan kadar glukosa darah yang bermakna antara kontrol negatif dengan dosis 90 mg/kgbb, dosis 360 mg/kgbb, dan kontrol positif, antara dosis 180 mg/kgbb dengan dosis 360 mg/kgbb dan kontrol positif. Analisis terhadap ekspresi AMPK-α1 pada hati tikus menunjukkan perbedaan yang bermakna antara dosis 180 mg/kgbb dengan dosis 360 mg/kgbb dan kontrol positif. Simpulan dari penelitian ini adalah ekstrak Singawalang dapat menurunkan kadar glukosa darah pada tikus model diabetes melitus melalui aktivasi AMPK-α1.Kata kunci: AMPK-α1, diabetes melitus, kadar glukosa darah, Singawalang Effect of Petiveria alliacea Leaves Extract in Decreasing Serum Level of Blood Glucose Level Through Activation of AMPK-α1 in Diabetes Mellitus Rat ModelsAbstractSingawalang (Petiveria alliaceae) is a medicinal herb that is used traditionally as folk medicine for various diseases. The plant has been used as an antidiabetic agent in Indonesian society. Until now, the effect and mechanism of action of Petiveria alliaceae as antidiabetic agent is not clear. The aim of the study was to determine the effect of Petiveria alliaceae to blood glucose level and to identify expression of AMPK-α1 in rat liver. The research was experimental study with randomized and was conducted at Laboratory Pharmacology and Therapy, and Laboratory Patology Anatomy, Faculty of Medicine, Universitas Airlangga, on April until August 2015. Twenty five male rats were injected by Streptozotocin to create diabetic rat models and randomly divided into 5 groups. The group 1, 2 and 3 are treatment groups that were given the ethanol extract of Petiveria alliaceae at dose 90 mg/kgbw, 180 mg/kgbw, and 360 mg/kgbw, orally, once daily for fourteen days. The fourth group is a negative control who were given distilled water and the fifth group is a positive control who were given metformin at dose 150 mg/kgbw. On the 15th day, blood glucose level were measured by glucometer and the rats were sacrificed to collect the liver. Expression of AMPK-α1 were assessed by immunohistochemistry. Data were analyzed by ANAVA and Wilcoxon (α=0,05). The results have been showed that there were significant differences in glucose blood level between negative control with the group were given the extract at dose 90 mg/kgbw, 360 mg/kgbw and positive control, between dose 180 mg/kgbw with dose 360 mg/kgbw and positive control. A significant differences of expression of AMPK-α1 showed between dose 180 mg/kgbw with dose 360 mg/kgbw and positive control. The conclusion of this study was the extract ethanol of Singawalang leaves reduce blood glucose level in diabetes mellitus rat models through increase in the expression of AMPK-α1.Keywords: AMPK-α1, blood glucose level, diabetes mellitus, Singawalang

Pengaruh Pemberian Virgin Coconut Oil terhadap Kadar Kreatinin dan Urea Nitrogen Darah Rattus Norvegicus Jantan NOVIANE ANGRELLA; ROOSTANTIA INDRAWATI; LESTARI DEWI
HANG TUAH MEDICAL JOURNAL Vol 17 No 2 (2020): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

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Abstract Background: Virgin Coconut Oil (VCO) is a functional product that is highly favored and have been widely applied in the everyday lives of society. The increase of the frequencies and duration of VCO consumption is suspected that VCO could negatively impact the target organ. The kidney is a vital organ in the human body, it is responsible for clearing out the residual waste products of metabolism in the body. The evalution of the kidney‟s function can be done through the measurement of creatinine and blood urea nitrogen. The purpose of this study is to determine the effect of VCO administration on kidney function. Method: 24 male wistar rats that are divided into 4 groups, which are listed as follows, K(-) or a negative-controlled group, P(1) or the first group of rats that are given VCO with a dose of 2mL/200gr of the rats‟ total BW/day, P(2) or the second group of rats that are given VCO with a dose of 3mL/200gr of the rats‟ total BW/day, and P(3) or the third group of rats that are given VCO with a dose of 4mL/200gr of the rats‟ total BW/day for 28 day Results: Administrating VCO with a dose of 2mL/200gr and 3mL/200gr of the rats‟ total BW/day does not affect the blood‟s creatinine level while with a dose of 4mL/200gr of the rats‟ total BW/day decreases the blood‟s creatinine level. Administrating VCO with a dose of 2 mL/200gr, 3 mL/200gr, and 4 mL/200gr of the rats‟ total BW/day does not affect the level of blood urea nitrogen. Conclusion: Administration of VCO dose of 4 mL / 200 g BW rat / day significantly decreased blood creatinine levels in experimental animals, while other doses did not affect the creatinine levels or blood urea nitrogen levels

Effect of Spirulina Platensis on Liver Malondialdehyde Levels of Male White Rat Induced by High Dose Paracetamol Anggilia Vanny; Sihning E. J. Tehupuring; Indri Ngesti Rahayu; Roostantia Indrawati
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 12, No 2 (2023): September 2023
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jikw.v12i2.2700

Paracetamol is widely used in therapy, yet it becomes hepatotoxic agent in a high dose using. The CYP450 enzyme metabolizes a small portion of paracetamol to a reactive substance, NAPQI. High dose of paracetamol causes an extensive increase in NAPQI levels. NAPQI then binds to hepatocytes conditions of oxidative stress. Spirulina platensis contains C-phycocyanin, flavonoids, and phenolics that inhibit lipid peroxidation and reduce oxidative stress. This study aims to determine the effect of Spirulina platensis on MDA levels in the liver tissue of male Wistar rats induced by high dose of paracetamol. This research was experimental with a Randomized Post-test Only Control Group design using 24 white male rats divided into three groups; negative control group (G-), positive control group (G+) was given paracetamol at a dose of 1,250 mg/kg on the 8th day, and treatment group (Gt) was given an amount of Spirulina platensis extract 360 mg/kg BW/day for ten days from 1st until 10th day and paracetamol at a single dose of 1,250 mg/kg BW on the 8th. At the 10th day of the treatment, all groups were terminated for liver tissue collection. TBA test was used to calculate MDA levels. Statistical tests analyzed the results of the data. This statistical tests by SPSS software: MDA levels in the treatment group did not significantly differ (p = 0.144) compared to the negative control group. There was a significant difference of liver tissue MDA levels in the treatment group (p = 0.016) compared to the positive control group. This study concludes that administering Spirulina platensis at a dose of 360 mg/kg/day for ten days can help prevent on increase in tissue MDA levels and maintain liver conditions close to their original state.

THE INFLUENCE OF HYPERBARIC OXYGEN THERAPY ON COCHLEAR PERCEPTION IMPROVEMENT IN SUDDEN DEAFNESS: LITERATURE REVIEW Zahira, Azzeldine Aliya; EP, Djati Widodo; Hisnindarsyah, Hisnindarsyah; Buwana, Aditya Wira; Indrawati, Roostantia
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 2 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i2.2536

Gangguan pendengaran mendadak diketahui menjadi salah satu gangguan pendengaran yang sering terjadi dengan jumlah kasus berkisar 80% hingga 90% kasus, dan mayoritas berdampak pada gangguan pendengaran mendadak di salah satu telinga. Kasus gangguan pendengaran mendadak diketahui dapat disembuhkan melalui manajemen yang sesuai. Kelainan vaskuler, kelainan imunologi, infeksi akibat virus serta rusaknya membran koklea dinilai sebagai faktor penyebab gangguan pendengaran. Salah satu manajemen gangguan pendengaran mendadak yang sering dilakukan dalam jangka waktu pendek adalah terapi oksigen hiperbarik. Terapi oksigen hiperbarik pada pasien gangguan pendengaran mendadak sebagai salah satu manajemen penanganan gangguan pendengaran mendadak bekerja melalui mekanisme peningkatan distribusi oksigen pada koklea. Penelitian ini disusun dengan tinjauan literatur sistematis untuk mengevaluasi pengaruh terapi oksigen hiperbarik terhadap perbaikan gangguan pendengaran mendadak. Peneliti memilih sampel dari jurnal internasional dan nasional yang terindeks oleh ProQuest, Wiley Online, Science Direct, Taylor Francis, SAGE, PubMed, dan SINTA antara tahun 2017 dan 2022. Peneliti menyadari kurangnya literatur yang relevan karena hanya 11 jurnal yang sesuai dengan kriteria penelitian. Namun, ini dianggap sebagai langkah awal untuk penelitian lebih lanjut. Temuan menunjukkan bahwa terapi oksigen hiperbarik berpengaruh terhadap perbaikan persepsi koklea pada pasien yang mengalami gangguan pendengaran mendadak, setelah pemberian terapi dengan rata-rata waktu 1-2 minggu, dengan tekanan 1,8 ATA hingga 2 ATA, baik dikombinasikan dengan kortikosteroid maupun terapi oksigen hiperbarik saja. Rekomendasi penulis adalah penelitian selanjutnya dapat meneliti faktor yang memengaruhi efektivitas pemberian terapi oksigen hiperbarik terhadap perbaikan gangguan pendengaran mendadak serta pemberian terapi oksigen hiperbarik dapat menjadi salah satu terapi adjuvan yang dapat diberikan kepada pasien di Indonesia.

Penatalaksanaan Pasien Penyakit Jantung Koroner Di RSPAL dr. Ramelan Surabaya ZULVINA, NADIFA FAULIZA; M. FATHUDDIN AL-ISLAMI; NABELLA CHOLIDATUN NIKMAH; NADHIFA NURIZZAH DHIYANISA; NADIFA SALMA SARI; ROOSTANTIA INDRAWATI; INTAN KOMALASARI
Hang Tuah Medical Journal Vol 22 No 2 (2025): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/htmj.v22i2.503

Background: World Health Organization (WHO) data shows that coronary heart disease is the highest mortality and morbidity throughout the world among other heart and vascular diseases, namely around 43%. The pharmacological therapy is evaluated based on the rationality of drug use including the right indication, the right drug, the right patient and the right dose. The aim of evaluating rational drug use is to ensure that patients receive treatment that suits their needs and at an affordable price. The rational use of drugs is important in the success of disease and improving the quality of health. Methode: The method in research uses an observational analytical method with a retrospective cross-sectional approach, with a purposive sampling technique. Data obtained from the patient's medical record status. The population in this study were all outpatients with a diagnosis of coronary heart disease in The Polyclinic Cardiology Department at RSPAL dr. Ramelan Surabaya period of January – March 2023. Result : The most patient characteristics based on range of age were in the 56 until 65 years old, namely 113 patients (33.43%). Characteristics of patients based on gender, the highest number of coronary heart medication users were men, 240 patients (71%). Characteristics of patients based on comorbidities (with comorbidities) were mostly with comorbidities, 296 patients (88%). The percentage of rational drugs of use includes the right indication (78.70%), the right drug (82.25%), the right dose (100%), and the right patient (99.11%). Conclusion: The rationale for using coronary heart medication in coronary heart patients in the outpatient installation at RSPAL dr. Ramelan Surabaya is 94.30% rational. Keywords: Coronary Heart Disease, Evaluation of Drug Use, Drug Evaluation, Outpatient.

Neuromuscular Taping Increases Muscle Strength, Flexibility, and Shortens The 100 Meter Sprinter Travel Time Endaryanto, Agung Hadi; Asnar, Elyana; Indrawati, Roostantia
Folia Medica Indonesiana Vol. 57, No. 1
Publisher : Folia Medica Indonesiana

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Lari sprint atau lari jarak pendek adalah salah satu nomor dalam atletik yang paling bergengsi, pelarinya disebut Sprinter. NMT adalah tehnik aplikasi yang menggunakan elastic adhesif tape yang memberikan kemampuan peningkatan fungsional otot, dan stimulasi sistem saraf. Tujuan penelitian ini, untuk mengetahui pengaruh neuromuscular taping terhadap kekuatan otot, fleksibilitas otot dan waktu tempuh sprinter 100 meter. Penelitian ini menggunakan metode pre test-post test with control grup design dengan total sampel berjumlah 13 orang dengan rentang usia atlet 15-19 tahun, pada tanggal 4-5 Agustus 2016 di GOR Gresik. Metode 1 kelompok dengan 2 perlakuan yaitu perlakuan I sebelum pemberian NMT dilakukan pengukuran kekuatan otot, fleksibilitas otot, dan waktu tempuh berlari 100 meter dan sesudah pemberian NMT dilakukan pengukuran kembali, perlakuan 2 sebagai kelompok kontrol dilakukan tanpa pemberian NMT dilakukan pengukuran kekuatan otot, fleksibilitas otot, dan waktu tempuh berlari 100 meter. Hasil penelitian menunjukkan pada perlakuan 1 kekuatan otot gastrocnemius dengan peningkatan rerata 11,38±7,83kg, nilai p=0,000, fleksibilitas nilai p=0,003 (dorsofleksi), p=0,013 (plantarfleksi) dengan peningkatan rerata 3,46±4,27 derajat, dan pemendekan waktu tempuh dengan nilai p=0-001, pada perlakuan 2 kekuatan otot gastrocnemius dengan penurunan rerata 2,12±2,43kg, nilai p=0,009, fleksibilitas nilai p=1,000 (dorsofleksi), p=0,165 (plantarfleksi) dengan peningkatan rerata 0,77±1,88 derajat, dan waktu tempuh terjadi perpanjangan waktu dengan nilai p=0-001 . Pemberian NMT pada sprinter memberikan hasil yang lebih baik dalam peningkatan kekuatan otot, fleksibilitas otot dan memperpendek waktu tempuh.

The Effect of Black Sea Cucumber (Holothuria atra) Ethanolic Extract and Gel on Diabetic Ulcers in Rattus norvegicus Induced by Streptozotocin Erika Nur Fanani; Roostantia Indrawati; Wienta Diarsvitri; Indri Ngesti Rahayu; Viranda Senora Permata; Nilam Cahya; Alifiah Wahyu Nur Fadilah
Majalah Biomorfologi Vol. 36 No. 1 (2026): MAJALAH BIOMORFOLOGI
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v36i1.2026.10-18

Background: Diabetic ulcer is a common complication of poorly controlled diabetes, often leading to chronic wounds and impaired healing. Black sea cucumber (Holothuria atra) is known to contain bioactive compounds, including saponins and flavonoids, which exhibit antidiabetic and wound-healing properties. Objective: To evaluate the therapeutic effect of ethanolic extract and gel formulations of Holothuria atra on diabetic ulcers in streptozotocin (STZ)-induced Rattus norvegicus. Materials and Methods: A randomized pre-test and post-test control group design was employed, involving 30 rats divided into five groups. Group C(-) served as the healthy control, while C(+) was a diabetic group treated with acarbose (4.5 mg/kg body weight) and 10% povidone-iodine. The experimental groups T1, T2, and T3 consisted of diabetic rats treated with Holothuria atra extract at doses of 50, 75, and 100 mg/kg body weight, respectively, along with corresponding topical gel concentrations (50%, 75%, and 100%) for 14 days. Ulcer healing was monitored by measuring wound closure, and data were analyzed using a one-way ANOVA test via IBM SPSS version 26.0 for Windows. Results: C(+) achieved the fastest wound closure by day 4, followed by T3 and T2 on day 5, and T1 on day 6. However, statistical analysis revealed no significant difference in ulcer healing between the groups (p = 0.56). Conclusion: Holothuria atra extract orally and gel topically demonstrated comparable healing efficacy to standard treatment with acarbose and povidone-iodine, although the differences were not statistically significant. These findings suggest Holothuria atra has potential as a complementary therapy for diabetic ulcers.

Effect of Ethanol Extract of Sea Pandan Fruit (Pandanus Tectorius) on Blood Glucose Levels in Male White Rats (Rattus Norvegicus) Induced by Dexamethasone Ghifara Kireina Annisa; Nita Pranitasari; R. Varidianto Yudo Tjahjono; Roostantia Indrawati
Indonesian Journal of Global Health Research Vol. 8 No. 3 (2026): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v8i3.1214

Diabetes mellitus is a metabolic disorder with increased blood glucose due to decreased insulin production or sensitivity. Chronic hyperglycemia can lead to endothelial dysfunction and vascular complications. Dexamethasone as a synthetic glucocorticoid can cause hyperglycemia through increased gluconeogenesis and insulin resistance. The antioxidant content in sea pandanus (Pandanus tectorius) has the potential to lower blood glucose levels. This study aims to determine the effect of ethanol extract of sea pandan fruit on blood glucose levels of male white rats (Rattus norvegicus) induced by dexamethasone. This study aims to determine the effect of ethanol extract of sea pandan fruit (Pandanus tectorius) on blood glucose levels in male white rats (Rattus norvegicus) induced by dexamethasone. Experimental research with a post test only control group on 30 mice grouped into 3 groups. Namely, K(-) which was only given standard feed, K(+) which was induced by dexamethasone at a dose of 5 mg/KgBB, and KP which was induced by dexamethasone at a dose of 5 mg/KgBB and given Pandanus tectorius fruit extract at a dose of 100 mg/KgBB, then at the end of the study a blood sample was taken to measure blood glucose levels and then terminated. The sample was selected using simple random sampling technique. The data was analyzed using the One Way Anova test. In the results of the study, the average blood glucose level K(-) = 170.37 was obtained; K(+) = 274.62; KP = 243. In the One Way Anova parametric test, a significant result was obtained which was 0.005 (p<0.05). Then in the Post Hoc Games-Howell test, K(-) and K(+) had a significance of 0.001 (p<0.05), while the K(-) and KP groups as well as K(+) and KP showed no significant results. This suggests that ethanol extract of Pandanus tectorius fruit did not successfully inhibit the increase in blood glucose levels in dexamethasone-induced white rats. The administration of ethanol extract of Pandanus tectorius fruit as a therapy was able to reduce the blood glucose level of male Rattus norvegicus induced by dexamethasone compared to a K(+) positive control of 31.62 mg/dL (11.5%).

Effectiveness of Ethanol Extract From Sea Pandan (Pandanus Tectorius) on Hydroxyproline Levels as A Parameter For Collagen Formation in The Healing Process of Incision Wounds in Male Rattus Norvegicus Vanessa Hedyana Putri; Roostantia Indrawati; Andi Abdullah; Afifah Sekarkusuma; Shehnaz Neisa Rasyid
Asian Journal of Social and Humanities Vol. 4 No. 4 (2026): Asian Journal of Social and Humanities
Publisher : Pelopor Publikasi Akademika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59888/ajosh.v4i4.674

This study aims to determine the effectiveness of ethanol extract gel of sea pandan fruit on hydroxycroline levels as a parameter for collagen formation in the healing process of incisional wounds of male white rats (Rattus norvegicus). This study is a laboratory experimental research with a post-test only control group design. The experimental animals used were male white rats which were divided into negative control groups (Gel Base), positive control (Bioplacenton®), and treatment groups with marine pandan ethanol extract gel concentrations of 10%, 20%, and 30%. The treatment was carried out for 10 days at the Integrated Biomolecular and Hyperbaric Laboratory, Faculty of Medicine, Hang Tuah University, Surabaya. The measured parameter was the level of hydroxychlorine of skin tissue. The results showed that there was a significant difference in hydroxyproline levels between treatment groups (p=0.032). The 20% concentration provides the most optimal effect with the highest average hydroxycroline levels and the narrowest data distribution, signaling more consistent fibroblast stimulation and collagen deposition. A concentration of 10% indicates high variability so the effect is less stable, while a concentration of 30% provides a moderate effect that may be affected by mild irritation. The conclusion of this study is that sea pandan fruit ethanol extract gel has the potential to accelerate the healing of incision wounds through increasing hydroxycrolin levels as an indicator of collagen formation.

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