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All Journal Jurnal Biologi Tropis Jurnal Medik Veteriner
I Wayan Wahyudi, I Wayan
Fakultas Ekonomi dan Bisnis Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Behavioral Patterns and Honey Yield Between Native and Non-native Stingless Bees in Bali's Ecosystem Wahyudi, I Wayan; Suardana, Anak Agung Komang
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8323

Abstract

The coronavirus disease 2019 (COVID-19) pandemic has led to significant growth in honey bee cultivation in Bali, leading to the introduction of nonnative bee species alongside the indigenous Tetragonula laeviceps. However, the 75% failure rate of nonnative species cultivation between 2020 and 2023 necessitated a deeper understanding of species adaptability in the Bali ecosystem. This study specifically examined the behavioral adaptation and honey production of Heterotrigona itama compared with those of Tetragonula laeviceps in a shared cultivation environment. A seven-month comparative experimental study involving 30 bee colonies (15 H. itama introduced from Lampung and 15 local T. laeviceps) was conducted. Time series sampling was employed at three daily intervals (09:00, 13:00, and 16:00) for behavioral observations, while honey production was measured via nondestructive vacuum extraction. H. itama demonstrated superior honey production (516.3 ml average) to that of T. laeviceps (213.3 ml average). The species exhibited distinct foraging preferences, with T. laeviceps favoring Jatropha integerrima and H. itama preferring Antigonon leptopus and Xanthostemon chrynsanthus. Both species have developed different adaptive strategies for nectar–water content management, with H. itama utilizing sun exposure and T. laeviceps employing smaller storage pots. This study revealed successful coexistence between native and nonnative species through differentiated foraging behaviors and resource utilization strategies, with H. itama showing greater productive capacity attributed to morphological and behavioral differences.
Co-Authors Anak Agung Komang Suardana Handayani, Ni Made I Ketut Suada I Made Damriyasa I Nyoman Arsana, I Nyoman I Wayan Masa Tenaya, I Wayan Masa Ida Ayu Pasti Apsari Kadek Karang Agustina Ni Ketut Ayu Juliasih Ni Luh Gede Sudaryati Sudiartawan, I Putu Sumarya, I Made TRI KOMALA SARI Widayantari, Anak Agung Ayu Sauca Sunia