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All Journal Jurnal Biologi Tropis Jurnal Medik Veteriner Svāsthya: Trends in General Medicine and Public Health
I Wayan Wahyudi, I Wayan
Fakultas Ekonomi dan Bisnis Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Behavioral Patterns and Honey Yield Between Native and Non-native Stingless Bees in Bali's Ecosystem Wahyudi, I Wayan; Suardana, Anak Agung Komang
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8323

Abstract

The coronavirus disease 2019 (COVID-19) pandemic has led to significant growth in honey bee cultivation in Bali, leading to the introduction of nonnative bee species alongside the indigenous Tetragonula laeviceps. However, the 75% failure rate of nonnative species cultivation between 2020 and 2023 necessitated a deeper understanding of species adaptability in the Bali ecosystem. This study specifically examined the behavioral adaptation and honey production of Heterotrigona itama compared with those of Tetragonula laeviceps in a shared cultivation environment. A seven-month comparative experimental study involving 30 bee colonies (15 H. itama introduced from Lampung and 15 local T. laeviceps) was conducted. Time series sampling was employed at three daily intervals (09:00, 13:00, and 16:00) for behavioral observations, while honey production was measured via nondestructive vacuum extraction. H. itama demonstrated superior honey production (516.3 ml average) to that of T. laeviceps (213.3 ml average). The species exhibited distinct foraging preferences, with T. laeviceps favoring Jatropha integerrima and H. itama preferring Antigonon leptopus and Xanthostemon chrynsanthus. Both species have developed different adaptive strategies for nectar–water content management, with H. itama utilizing sun exposure and T. laeviceps employing smaller storage pots. This study revealed successful coexistence between native and nonnative species through differentiated foraging behaviors and resource utilization strategies, with H. itama showing greater productive capacity attributed to morphological and behavioral differences.
Exposure to electromagnetic waves for reducing brain health: A bibliometric analysis Savitri, Ida Ayu Gede Winda; Adnyana, I Made Dwi Mertha; Wahyudi, I Wayan; Dewi, Gusti Ayu Putu Wahyu Purnama; Ariastuti, Indria; Sundari, Ni Luh Putu Ari; Nuartha, Pande Komang Aditya Ryan
Svāsthya: Trends in General Medicine and Public Health Vol. 1 No. 1 (2024): July 2024
Publisher : PT. Mega Science Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70347/svsthya.v1i1.12

Abstract

Exposure to electromagnetic waves has been reported to have an adverse impact on human brain health. Various claims have revealed the impact of long-term exposure to electromagnetic waves clinically and nonclinically, but there are no comprehensive studies that explain the effects of electromagnetic wave exposure on human brain health.  This study aimed to identify the effects of electromagnetic wave exposure on human brain health. Using a meta-synthesis approach and bibliometric analysis. A total of 271 articles from Scopus, Web of Science and PubMed were screened to obtain 148 terms and analyzed descriptively. The findings from 2004–2024 articles that met the selection criteria resulted in 10 major clusters, 903 networks and 4,020 network strengths. The terms "1800 MHz mobile phone”, "900 MHz”, "acute exposure”, "assessment”, "behavior”, "bioeffect”, "biological effect”, "biological tissue”, "blood brain barrier”, "cell phone radiation”, "cellular phone”, "cellular telephone”, "central nervous system”, "child”, "chronic exposure" and others were most studied. Increased use of electronic devices and wireless technology significantly affects information processing and storage; disrupts synaptic communication and neuronal signaling; causes cognitive decline; disrupts the integrity of the blood‒brain barrier; and causes hypersensitivity through disruption of monoamine neurotransmitter metabolism, decreased dopamine levels, norepinephrine and epinephrine levels, 5-hydroxytryptamine "serotonin", excitatory amino acid neurotransmitters and acetylcholine esterase (AChE). The increased use of electronic devices and wireless technology significantly affects brain performance and health. Thus, it is important to minimize the effects of exposure to electromagnetic waves.
Corticosteroids and glaucoma: How do treatments trigger ocular nerve damage? - A systematic literature review Putra, I Gede Satya Wijaya; Adnyana, I Made Dwi Mertha; Wahyudi, I Wayan; Suardana, Anak Agung Komang; Indrayanti, Ni Komang Rina; Prabowo, Sang Kompyang Yudistira Eddy; Panduyasa, I Gede; Widyantari, Dewa Ayu; Agustini, Desak Gede Dwi; Suwandani, Ni Made Ayu
Svāsthya: Trends in General Medicine and Public Health Vol. 1 No. 3 (2024): November 2024
Publisher : PT. Mega Science Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70347/svsthya.v1i3.22

Abstract

Corticosteroids are steroid hormone derivatives produced by the adrenal glands. Corticosteroids in the health sector have been widely utilized as anti-inflammatory agents because of their strong and rapid effects. This study aimed to identify the impact of long-term corticosteroid use on eye damage. This study uses a systematic literature review, which is guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) to the article screening process.  The data of this study, in the form of demographic variables, route of administration, steroid type, comorbidities, and patient risk factors, were descriptively analyzed. As a result, 15 of the 22 articles were selected for analysis. Chronic corticosteroid administration may precipitate optic neuropathy, manifesting as glaucoma, which is characterized by progressive structural degeneration of retinal ganglion cells (RGCs) and concomitant visual function deterioration. The locus of primary pathological insult in glaucomatous conditions is the optic nerve head, specifically at the optic disc. This anatomical site is notable for the abrupt 90-degree angular deviation of RGC axons as they transition from the retinal nerve fiber layer to their trajectory within the optic nerve proper. The use of corticosteroids can damage ocular nerve tissue through an increase in intraocular pressure (IOP) beyond 21 mmHg. This increase in IOP is due to changes in the microstructure of the trabeculum webbing, resulting in increased obstruction to the outflow of aqueous humor.
Co-Authors Adnyana , I Made Dwi Mertha Agustini, Desak Gede Dwi Anak Agung Komang Suardana Ariastuti, Indria Dewi, Gusti Ayu Putu Wahyu Purnama Handayani, Ni Made I Ketut Suada I Made Damriyasa I Nyoman Arsana, I Nyoman I Wayan Masa Tenaya, I Wayan Masa Ida Ayu Pasti Apsari Indrayanti, Ni Komang Rina Kadek Karang Agustina Ni Ketut Ayu Juliasih Ni Luh Gede Sudaryati Nuartha, Pande Komang Aditya Ryan Panduyasa, I Gede Prabowo, Sang Kompyang Yudistira Eddy Putra, I Gede Satya Wijaya Savitri, Ida Ayu Gede Winda Sudiartawan, I Putu Sumarya, I Made Sundari, Ni Luh Putu Ari Suwandani, Ni Made Ayu TRI KOMALA SARI Widayantari, Anak Agung Ayu Sauca Sunia Widyantari, Dewa Ayu