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All Journal Jurnal Sain Veteriner Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan
TRI KOMALA SARI
Virology Laboratory Faculty Of Veterinary Medicine Udayana University Bali
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Shedding Virus Vaksin Flu Burung Subtipe (H5N1) Isolat Dari Bali Tidak Ditemukan Pascavaksinasi Ayam Petelur Kencana, Gusti Ayu Yuniati; Sari, Tri Komala; Wijaya, Dhyana Ayu Manggala; Suartha, I Nyoman; Kendran, Anak Agung Sagung
Indonesia Medicus Veterinus Vol 10 (6) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.6.830

Abstract

Flu burung atau Avian influenza (AI) merupakan penyakit viral akut yang sudah tersebar luas di seluruh dunia, dan saat ini bersifat endemik di Indonesia. Avian Influenza digolongkan penyakit menular strategis prioritas karena bersifat zoonosis berbahaya yang dapat menyerang unggas dan mamalia maupun manusia. Unggas yang diternakkan secara massal lebih rentan terserang avian influenza, seperti ayam petelur, sehingga perlu dilakukan upaya pencegahan dengan vaksinasi. Virus avian influenza mudah mengalami mutasi sehingga tidak dapat dikenali oleh antibodi yang sudah ada di dalam tubuh unggas, oleh karena itu perlu untuk selalu dilakukan pengembangan vaksin, contohnya adalah vaksin avian influenza subtipe H5N1. Tujuan penelitian ini untuk mengetahui apakah vaksin AI-H5N1 isolat dari Bali dapat digunakan pada peternakan ayam komersial dengan melihat kemamampuannya menekan shedding virus. Penelitian ini dilakukan dengan mengambil 20 sampel swab kloaka secara acak dari 40 ekor ayam petelur yang sudah divaksin Avian Influenza subtipe H5N1 isolat dari Bali. Sampel swab selanjutnya diinokulasikan pada telur ayam berembrio melalui ruang alantois, diinkubasi dalam inkubator suhu 370C. Setelah 2-3 hari pascainokulasi, cairan alantois dipanen dan dilakukan uji HA/HI. Hasil penelitian menunjukkan tidak ditemukannya shedding virus vaksin AI-H5N1 isolat dari Bali, ditandai dari semua sampel swab yang diambil pada periode 1-4 minggu pascavaksinasi menunjukkan hasil negatif pada uji HA.
Respon Imun Seluler Ayam Petelur Pascavaksinasi Avian Influenza Subtipe H5N1 Isolat dari Bali Gusti Ayu Yuniati Kencana; Tri Komala Sari; I Nyoman Suartha; I Ketut Tomy Caesar Ramanda; Anak Agung Sagung Kendran
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.66086

Abstract

Avian Influenza subtype H5N1 (AI-H5N1) is a malignant virus that is very detrimental to laying chickens because it is highly contagious and mutates easily. Prevention of AI-H5N1 disease in laying chickens is carried out by vaccination, therefore to maintain the quality of the vaccine, continuous research is needed. This study aims to determine the potential of AI-H5N1 vaccine isolates from Bali as measured based on cellular immune response based on total and differential leukocyte cells. Formation of antibodies is influenced by the nonspecific and specific immune system involving leukocytes, especially lymphocytes. Total of 40 layers of Novogen Brown strain were used for the research sample, kept since the age of one day on a commercial farm in Perean Village, Tabanan Regency, Bali. Laying chickens are vaccinated at 5 weeks of age by intramuscular injection. Total of 20 chikens were taken randomly and used for the sample. Blood draws were performed once pre-vaccination and five times each week after vaccination with anticoagulants. Total leukocytes were examined by an auto hematology analyzer, while differential leucocytes with thin blood smear stained with Giemsa. Total and differential leukocyte data were analyzed by means of the variance test followed by the Duncan test. Results showed that AI-H5N1 vaccination from Bali isolates could increase total and differential leucocytes of laying chickens and had significant effect on the mean total leukocytes, the absolute values of heterophyll cells, eosinophils, lymphocytes, and monocytes, but had no significant effect on post-vaccination basophil cells.
Pola Distribusi Unggas dari Pasar Tradisonal Berperan dalam Penyebaran Virus Flu Burung I MADE SUMA ANTARA; I NYOMAN SUARTHA; I KADEK SAKA WIRYANA; I MADE SUKADA; I WAYAN WIRATA; I GUSTI NGURAH DIBYA PRASETYA; NI MADE RITHA KRISNA DEWI; TRI KOMALA SARI; I GUSTI NGURAH KADE MAHARDIKA
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (727.869 KB)

Abstract

A study has been carried out to map the distribution pattern of poultry from traditional market toreduce the transmission risk of avian influenza virus. The data were collected from threes markets wherepoultry are sold, namely in Bringkit of Badung Regency, Kumbasari of Denpasar City, and Kediri ofTabanan Regency. Data collections was based on interviews using questionnaire. Poultry from all marketsare distributed throughout Bali. Poultry are traded mainly for religious ceremony and immediatelyslaughtered as it arrives at the consumer’s house. The distribution pattern of poultry seems to play asignificant role in the disseminations of avian influenza virus. The right implementation of biosecurity intraditional markets is highly recommended to curb the risk.
Deteksi Virus Classical Swine Fever di Bali dengan RT-PCR I Wayan Wirata; Ida Ayu Sri Chandra Dewi; I Gusti Ngurah Narendra Putra1,; Ida Bagus Oka Winaya; Ida Bagus Kade Suardana3,; Tri Komala Sari; I Nyoman Suartha; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.782 KB)

Abstract

Classical Swine Fever (CSF) virus has been confirmed for the first time in pig in Bali. The object of thisstudy was suspected CSF cases diagnosed at the diagnostic laboratory assistantship of the Faculty ofVeterinary Medicine, Udayana University, in 2007-2008. Total number of cases was 12. Case recordsincluded the signalment of case (breed, age, body weight, and the origin of respective case), clinical signs,post-mortem lesions, and histological pictures. CSF virus was confirmed using the standardized reversetranscriptase-polymerase chain reaction (RT-PCR) for CSF from European Union. One RT-PCR productwas sequenced. CSF virus was confirmed in seven out of 12 cases (58%). The cDNA sequence wasconfirmed to be specific of CSF E2 protein coding region with 98% homology to one isolate from China thatwas available in GeneBank. Further works are recommended to elucidate the sensitivity of RT-PCR, toclarify some differential diagnose, and to find out the genetic variation of CSF virus in Bali.Key words: classical swine fever virus, Bali, RT-PCR
Survei Penyakit Porcine Reproductive and Respiratory Syndrome pada Peternakan Babi di Bali (SEROLOGICAL SURVEILLANCE OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME IN PIGGERIES IN BALI) I Nyoman Suartha; I Made Suma Anthara; I Wayan Wirata; Tri Komala Sari; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Narendra; I Gusti Ngurah Mahardika
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.951 KB)

Abstract

This study aimed to determine the presence and burden of Porcine Respiration and ReproductiveSyndrome (PRRS) virus in pig farms in Bali. A total of 305 sera samples were collected from 10 intensivepig farms and backyard piggeries located in eight districts, in Bali. The PRRS antibody and the virus wasdetected using enzyme linked immunosorbent assay (ELISA) and transverse reverse polymerase chainreaction (RT-PCR), respectively. The results showed that, generally the average percentage of positiveswine anti-PRRS antibody was 13.4%, 14.3%,  and 11.7% in the backyard farms and commercial farms,respectively. Whereas, the detection rate of PRRS virus was 8.9% (15.3% and 5.6% in the backyard farmand commercial farms, respectively). It was concluded that PRRS virus is endemic in pigs, in Bali.Vaccination, management, biosafety, and quarantine  should be implemented to prevent the economicloss due to PRRS.
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
MOLECULAR MORPHOLOGY OF SNAKE VENOM PROTEIN Trimeresurus insularis AFTER FREEZE DRYING DETECTED BY SCANNING ELECTRON MICROSCOPE Adrianto, Steven; Sari, Tri Komala; Suarsana, I Nyoman; Suartini, I Gusti Ayu Agung
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.41219

Abstract

The high protein content in snake venom make venom samples prone damaged. Freeze drying is a solution to stabilize the protein in snake venom and the addition of excipients such as sugar and surfactant, can prevent negative impacts during freeze drying process. The purpose of this study was to examine the morphology of Trimeresurus insularis (T. insularis) snake venom protein molecules after freeze-drying with the addition of sucrose, Tween 80, and PBS (pH 7.2). In this study, venom from nine T. insularis snakes was used. Before freeze drying, the treated samples were supplemented with sucrose, Tween 80, and PBS (pH 7.2), while, the control sample was only supplemented with PBS (pH 7.2). After freeze drying, the morphology of both samples was observed using a scanning electron microscope at magnification of 100x and 3000x. The result showed that the control sample was damaged and resembled broken glass, whereas the treatment sample, although it also appeared as shattered glass, was more intact and exhibited finer and smaller flakes.
Landrace Swine Mortality Due to Colibacillosis in Conventional Farming Yasa, Gede Sastra Darma; Tono PG, I Ketut; Winaya, Ida Bagus Oka; Apsari, Ida Ayu Pasti; Sari, Tri Komala
Journal of Applied Veterinary Science And Technology Vol. 6 No. 2 (2025): October 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I2.2025.178-184

Abstract

Background: Colibacillosis, caused by Escherichia coli infection, is a major health issue in pig farming, leading to high morbidity and mortality rates. This report describes a case that occurred on a conventional pig farm in Puhu Village, Gianyar District, Bali. Purpose: This study aimed to determine the cause of death in a 43 day old Landrace pig exhibiting clinical signs including brown watery diarrhea, dehydration, and weight loss. Case(s): A necropsy was conducted at the Veterinary Pathology Laboratory, Faculty of Veterinary Medicine, Udayana University. Bacteriological examination was performed by culturing organ samples on Nutrient Agar and Eosin Methylene Blue Agar, followed by biochemical testing using Triple Sugar Iron Agar and Sulfide Indole Motility media, among others. Parasitological examination was carried out using the fecal float concentration method. Case Management: Epidemiological assessment revealed morbidity, mortality, and case fatality rates of 1.89%, 0.63%, and 33.33%, respectively. Gross pathological findings included intestinal and gastric dilation and hemorrhage, intestinal edema, and lesions in the brain, trachea, lungs, heart, liver, and kidneys. Histopathological examination revealed localized intestinal congestion, generalized cardiac congestion, and inflammatory cell infiltration in the intestinal mucosa. Bacteriological analysis confirmed Escherichia coli with alpha-hemolytic activity on blood agar. Eimeria spp. oocysts were not detected in the fecal samples. Conclusion:  The pig’s death was attributed to colibacillosis caused by Escherichia coli infection. This case highlights the importance of early detection, strict biosecurity measures, and proper sanitation practices to prevent disease outbreaks in conventional pig farming.
Co-Authors Adrianto, Steven Anak Agung Komang Suardana Anak Agung Sagung Kendran Gusti Ayu Yuniati Kencana Handayani, Ni Made I Gusti Agung Ayu Suartini I GUSTI NGURAH DIBYA PRASETYA I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra I Gusti Ngurah Narendra Putra1, I KADEK SAKA WIRYANA I Ketut Suada I Ketut Tomy Caesar Ramanda I Made Damriyasa I Made Sukada I MADE SUMA ANTARA I Made Suma Anthara I Nyoman Arsana, I Nyoman I Nyoman Suarsana I Nyoman Suartha I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan I Wayan Wirata Ida Ayu Pasti Apsari Ida Ayu Sri Chandra Dewi Ida Bagus Kade Suardana Ida Bagus Oka Winaya Kadek Karang Agustina Ni Ketut Ayu Juliasih Ni Luh Gede Sudaryati Ni Made Ritha Krisna Dewi Sudiartawan, I Putu Sumarya, I Made Tono PG, I Ketut Widayantari, Anak Agung Ayu Sauca Sunia Wijaya, Dhyana Ayu Manggala Yasa, Gede Sastra Darma