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All Journal Journal Of Vocational Health Studies Jurnal Medik Veteriner Jurnal Bali Membangun Bali Berita Kedokteran Masyarakat Journal of Tropical Pharmacy and Chemistry
Sudiartawan, I Putu
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EVALUATION OF REFILLABLE DRINKING WATER QUALITY BASED ON MPN COLIFORM AND ESCHERICHIA COLI IN SESETAN VILLAGE, SOUTH DENPASAR, BALI Wyasena, Pande Nyoman Tri Saguna; Sudaryati, Ni Luh Gede; Sudiartawan, I Putu; Adnyana, I Made Dwi Mertha
Journal of Vocational Health Studies Vol. 6 No. 2 (2022): November 2022 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V6.I2.2022.93-101

Abstract

Background: Refilled drinking water given by depots that currently have a permit for operation can be used to meet the community's drinking water demands. However, the quality of refilled drinking water has been tainted with pathogens that can cause health problems. Purpose: To assess the quality of replenished drinking water in Sesetan Village using Most Probable Number (MPN) Coliform and Escherichia coli as indicators. Method: A qualitative approach to descriptive observational research. The Ministry of Health standard 492/Menkes/PER/IV/2010 is used to assess drinking water quality. In this investigation, ten samples were used. Sampling was conducted in Sesetan Village, South Denpasar, Bali. Result: MPN Coliform was found in four samples: 96 MPN/100 mL (X1), 38 MPN/100 mL (X7), 15 MPN/100 mL (X8), and 5 MPN/100 mL (X10). Meanwhile, all of the samples tested negative for Escherichia coli. Conclusion: Based on drinking water quality criteria, 4 (40%) refilled drinking water depots (DAMIU) did not meet quality requirements (Ministry of Health Regulation number 492/Menkes/PER/2010).
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Community perception of biocontrol agent using Betta splendens (Ikan Cupang) against Aedes aegypti larvae: a community study in Bali Sudiartawan, I Putu; Erjana , I Gede Putra; Juliasih , Ni Ketut Ayu; Arsana , I Nyoman
BKM Public Health and Community Medicine Vol 39 No 06 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bkm.v39i6.4262

Abstract

Purpose: This study attempted to analyze the predation ability of Betta splendens as a biocontrol of Aedes aegypti larvae with a case study in Kartika Neighborhood, Dauh Puri, Denpasar City. Methods: This study employed a quasi-experimental approach and a qualitative investigation to ascertain public perceptions of using Betta splendens as a biocontrol agent for Aedes aegypti larvae. The trial lasted one month. Betta splendens were added to the respondent's bath (n=30), and larval mortality was determined at 2, 4, 6, 8, and 10 hours. Analysis of data using the paired t-test. Results: The results showed that the characteristics of respondents were dominated by being 18–45 years old (73.30%), being male (56.70%), having a dominant education level of high school (66.60%), and using a cement bathtub (53.30%). The comparative test results obtained a p-value of 0.0001 (p<0.05), indicating a very significant change in the number of Aedes aegypti larvae during bathing before and after adding Betta fish, with an average predation ability of 85.87%. Community perception regarding using Betta fish as a biocontrol agent is very positive, and the acceptance rate and sustainability of Betta fish use reached 96.67%. There is no change in water conditions due to aroma, color, and taste. Conclusion: The predation power of Betta splendens as a natural biocontrol is excellent and effectively utilized in minimizing the presence of Aedes aegypti larvae in the bath (container).
Pengobatan Tradisional Bali Usadha Tiwang Arsana, I Nyoman; Sudiartawan, I Putu; Sudaryati, Ni Luh Gede; Wirasuta, I Made Agus Gelgel; Armita, Pande Made Nova; Warditiani, Ni Kadek; Astuti, Ni Made Widi; Santika, I Wayan Martadi; Wiryanatha, Ida Bagus; Cahyaningrum, Putu Lakustini; Suta, Ida Bagus Putra
Bali Membangun Bali: Jurnal Bappeda Litbang Vol 1 No 2 (2020): August 2020
Publisher : Badan Riset dan Inovasi Daerah Provinsi Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51172/jbmb.v1i2.113

Abstract

Tujuan: Artikel ini bertujuan untuk mengkaji pengobatan tradisional Bali, dengan mengambil fokus kajian pada pengobatan yang tertuang dalam lontar Usadha Tiwang. Metode penelitian: Penelitian ini menggunakan metode kepustakaan (Library Research). Unit analisis berupa naskah lontar Usadha Tiwang, yang telah ditransliterasi dari aksara Bali ke aksara Latin. Temuan: Hasil penelitian diketahui bahwa tiwang adalah penyakit yang mempunyai gejala badan terasa meluang, sakit dan ngilu, gelisah, mata mendelik, otot kaku bahkan sampai pingsan. Jenis tiwang dicirikan berdasarkan gejala yang muncul. Pengobatan dilaksanakan secara holistik oleh pengusada sesuai tatalaksana pengusada, dengan menggunakan ramuan obat-obatan yang terbuat dari campuran berbagai jenis tumbuh-tumbuhan atau bahan lainnya seperti arak, lengis tanusan, garam, gula, kapur, maupun santen, bahkan tain seksek serta iduh bang. Penggunaannya dengan cara dimakan, diminum, ditutuhkan, disemburkan, diuapkan atau dilulurkan, maupun ditempelkan. Takaran, cara pengolahan, serta cara pemakaian masih belum jelas. Implikasi: Masyarakat Bali tetap percaya terhadap sistim pengobatan tradisional Bali. Namun demikian, masyarakat Bali yang berobat ke tempat praktik pengobatan tradisional sangat sedikit. Simpulannya adalah pengobatan tradisional Bali dilakukan secara holistik untuk mencapai keseimbangan antara shtula sarira-suksma sarira-antahkarana sarira.
Formulation of the Balm Aromatherapy Combination Using the Essential Oils Ocimum basilicum L. and Cymbopogon citratus DC Cahyaningrum, Putu Lakustini; Suta, Ida Bagus Putra; Sudiartawan, I Putu; Saraswati, Ida Ayu Asri Diva; Dirgayasa, Pande Nyoman Selamet
Journal of Tropical Pharmacy and Chemistry Vol. 8 No. 1 (2024): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v8i1.518

Abstract

This study aimed to analyze the formulation of an aromatherapy balm composed of the essential oils O. basilicum L. and C. citratus DC. Experimental research design involving the compilation of five balm formulations at concentrations of 0, 5, 10, 15, and 20%. For four months, balm formulations were made, and physical, chemical, and microbiological tests were carried out, which were further analyzed descriptively. The findings showed that the yield of essential oils obtained from distillation reached 0.19% w/v (O. basilicum L.) and 0.24% w/v (C. citratus DC). All formulations at concentrations of 0, 5, 10, 15, and 20% passed the organoleptic tests for topical dosage balms. This means that they were semisolid, smelled like essential oils, and were yellowish-brown based on the raw materials. The balm is completely homogeneous; there are no granules, and the color is evenly spread throughout the smear. The pH for the topical preparations ranged from 6–7, the dispersion power ranged from 5.02–6.10, and the adhesion ratio ranged from 2–6 depending on the concentration. Microbiological testing revealed that the aromatherapy balm was free of S. aureus and P. aeruginosa bacteria.
Co-Authors Adnyana , I Made Dwi Mertha Anak Agung Komang Suardana Armita, Pande Made Nova Arsana , I Nyoman Astuti, Ni Made Widi Dirgayasa, Pande Nyoman Selamet Erjana , I Gede Putra Handayani, Ni Made I Ketut Suada I Made Damriyasa I Nyoman Arsana, I Nyoman I Wayan Martadi Santika I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan Ida Ayu Pasti Apsari Ida Bagus Wiryanatha Juliasih , Ni Ketut Ayu Kadek Karang Agustina Ni Kadek Warditiani Ni Ketut Ayu Juliasih Ni Luh Gede Sudaryati Putu Lakustini Cahyaningrum Saraswati, Ida Ayu Asri Diva Sumarya, I Made Suta, Ida Bagus Putra TRI KOMALA SARI Widayantari, Anak Agung Ayu Sauca Sunia Wyasena, Pande Nyoman Tri Saguna