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All Journal Journal Of Vocational Health Studies Jurnal Medik Veteriner
Sudiartawan, I Putu
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Biochemistry, Genetics & Molecular Biology Health Professions Veterinary

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EVALUATION OF REFILLABLE DRINKING WATER QUALITY BASED ON MPN COLIFORM AND ESCHERICHIA COLI IN SESETAN VILLAGE, SOUTH DENPASAR, BALI Wyasena, Pande Nyoman Tri Saguna; Sudaryati, Ni Luh Gede; Sudiartawan, I Putu; Adnyana, I Made Dwi Mertha
Journal of Vocational Health Studies Vol. 6 No. 2 (2022): November 2022 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V6.I2.2022.93-101

Abstract

Background: Refilled drinking water given by depots that currently have a permit for operation can be used to meet the community's drinking water demands. However, the quality of refilled drinking water has been tainted with pathogens that can cause health problems. Purpose: To assess the quality of replenished drinking water in Sesetan Village using Most Probable Number (MPN) Coliform and Escherichia coli as indicators. Method: A qualitative approach to descriptive observational research. The Ministry of Health standard 492/Menkes/PER/IV/2010 is used to assess drinking water quality. In this investigation, ten samples were used. Sampling was conducted in Sesetan Village, South Denpasar, Bali. Result: MPN Coliform was found in four samples: 96 MPN/100 mL (X1), 38 MPN/100 mL (X7), 15 MPN/100 mL (X8), and 5 MPN/100 mL (X10). Meanwhile, all of the samples tested negative for Escherichia coli. Conclusion: Based on drinking water quality criteria, 4 (40%) refilled drinking water depots (DAMIU) did not meet quality requirements (Ministry of Health Regulation number 492/Menkes/PER/2010).
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Co-Authors Adnyana , I Made Dwi Mertha Anak Agung Komang Suardana Handayani, Ni Made I Ketut Suada I Made Damriyasa I Nyoman Arsana, I Nyoman I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan Ida Ayu Pasti Apsari Kadek Karang Agustina Ni Ketut Ayu Juliasih Ni Luh Gede Sudaryati Sumarya, I Made TRI KOMALA SARI Widayantari, Anak Agung Ayu Sauca Sunia Wyasena, Pande Nyoman Tri Saguna