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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Kedokteran Diponegoro Althea Medical Journal Molecular and Cellular Biomedical Sciences (MCBS) International Journal of Cell and Biomedical Science
Prasetyowati Subchan, Prasetyowati
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience

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PENGARUH SANITASI LINGKUNGAN TERHADAP PREVALENSI TERJADINYA PENYAKIT SCABIES DI PONDOK PESANTREN MATHOLIUL HUDA AL KAUTSAR KABUPATEN PATI Mayrona, Cindy Tia; Subchan, Prasetyowati; Widodo, Aryoko
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 7, No 1 (2018): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.59 KB) | DOI: 10.14710/dmj.v7i1.19354

Abstract

Latar Belakang: Di Indonesia, angka kejadian penyakit skabies mencapai 5,6-12,95%. Pesantren sebagai tempat yang sering didapati higiene perorangan kurang memadai, tentu menjadi tempat yang sesuai untuk penularan penyakit skabies. Angka kejadian skabies sendiri di Pondok Pesantren di Demak mencapai 45,5%.Tujuan: Mengetahui Pengaruh Sanitasi Lingkungan terhadap Prevalensi Terjadinya Penyakit Skabies di Pondok Pesantren Matholiul Huda Al Kautsar Kabupaten Pati.Metode: Penelitian ini menggunakan rancangan penelitian observasional dengan pendekatan cross sectional. Subjek penelitian ini adalah 46 santri yang memenuhi kriteria inklusi dan ekslusi di Pesantren Matholiul Huda Al Kautsar Kabupaten Pati. Pengumpulan data menggunakan kuesioner. Analisa data menggunakan uji chi-square.Hasil: 31 santri (67,4%) memiliki praktik sanitasi lingkungan yang buruk dan 15 santri (32,6%) memiliki praktik sanitasi lingkungan yang baik. Dari 46 santri ditemukan 39 santri (84,8%) yang menderita skabies. Dengan uji chi square didapatkan nilai p 0,029 ( p<0,05) maka secara statistik terdapat pengaruh yang signifikan antara praktik sanitasi lingkungan dengan kejadian skabies. Hasil perhitungan Prevalence Ratio (PR) diperoleh nilai 0,7 yang berarti bahwa santri yang praktik sanitasi lingkungan yang buruk mempunyai resiko 0,7 kali untuk mendrita skabies dibanding dengan santri yang praktik sanitasi lingkungannya baik.Kesimpulaan : Ada pengaruh yang signifikan antara praktik sanitasi lingkungan dan kejadian skabies di pesantran Matholiul Huda Al Kautsar Kabupaten Pati. 
The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis Soetrisno, Kusumawati; Subchan, Prasetyowati; Hussana, Atina
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (880.582 KB) | DOI: 10.30659/sainsmed.v11i2.9094

Abstract

Introduction: Ultraviolet B (UVB) radiation triggers the formation of free radicals that cause apoptosis and sunburn cells (SBC) formation. Aloe vera contains anti-inflammatory and antioxidant compounds that can potentially inhibit this process. Objective: to assess the effect of topical Aloe vera extract administration on the decrease of SBC number and Caspase-3 expression on the epidermis after UVB light exposure.Methods: In a post-test only group design study, a single dose of 3 doses of Erythema (DEM) was performed on two groups of 6 week old BALB/c female rats. Group A (control) did not receive any topical treatment, and group B (treatment) were smeared with 75% Aloe vera extract before irradiation. Each group was divided into 4 sub-groups based on post-exposure time of 6 hours (A1 and B1), 12 hours (A2 and B2), 24 hours (A3 and B3), and 48 hours (A4 and B4). The expression of caspase-3 was assessed by immunohistochemical staining while the SBC number was measured using a microscope. The expression of caspase-3 was analyzed using the Kruskal-Wallis and Mann-Whitney statistical tests, while the number of SBC was analyzed using the one-way ANOVA statistical test and the post-hoc LSD test.Results: The results of the Kruskal-Wallis analysis showed a significant difference in Caspase-3 expression between groups, p <0.05. The results of Mann-Whitney analysis showed a significant difference in Caspase-3 expression between the A1-A3 and B1-B3 subgroups (p <0.05). One-way ANOVA analysis showed a significant difference in the number of SBC between groups (p <0.05). Post-hoc LSD analysis showed significant differences in SBC counts between groups A and B across all subgroups, p <0.05.Conclusion: Topical application of Aloe Vera extract decreased Caspase-3 expression and the number of SBC in UVB light-exposed skin.
Topical Gel of Mesenchymal Stem Cell-Conditioned Medium-induced Serum Injury Accelerates Wound Healing in Skin Excision Tissue Berlian, Mukti Arja; Alif, Iffan; Subchan, Prasetyowati; Handoyo, Frigi Eko; Husain, Sofian Azalia; Husni Ahmad Sidiq; Arlinda, Dyken Dwi; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.12

Abstract

Introduction: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) accelerating wound closure by increasing VEGF and PDGF level leading to re-epithelialization, cell infiltration, and angiogenesis. It has been found that MSC-conditioned medium (MSC-CM) can enhance migration of fibroblasts in scratch assays. However, the effect of MSC-CM-induced serum injury (MSC-CM-S) formulated in gel to accelerate wound healing remains unclear. This study aims to evaluate the effect of several doses of topical gel of MSC-CM-S in accelerating wound healing. Methods: The MSCs were cultured medium-supplemented serum injury of wounded rat (8:1) to get MSC-CM-S. The topical gel of MSC-CM-S was made by base gel supplemented with MSC-CM-S. Eighteen Wistar rats were randomly assigned into control (C) and treatment groups (T1, T2). Groups were received serum-free medium gel (C), 25 µl MSC-CM-S in topical gel (T1), 50 µl MSC-CM-S in topical gel (T2), twice daily for 9 days. PDGF and VEGF level and fibroblast density were measured by ELISA and HE staining at day 3 and 6, respectively. Results: This study showed that there was significant increase of VEGF and PDGF level along with a significant increase of fibroblast density at day 3 and 6. The T2 showed optimum enhancement level of VEGF, PDGF and fibroblast density. Conclusion: Topical gel of MSC-CM-S was effective to accelerate wound closure by enhancing PDGF and VEGF level in full-thickness skin defect rats.
Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree Burns Hariani, Nova Putri; Putra, Agung; Subchan, Prasetyowati; Setiawan, Eko
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.635

Abstract

Background: Second-degree burns cause extensive damage to the skin and pose significant health challenges, with current treatments facing limitations such as donor skin shortages and complications. Fibroblast growth factor 1 (FGF-1) and stromal-derived growth factor 1 (SDF-1) are critical for tissue repair. Emerging evidence suggests that mesenchymal stem cell-derived exosomes (E-MSCs) are a promising cell-free therapeutic option for enhancing wound healing through the modulation of FGF-1 and SDF-1. This study investigated the effect of E-MSCs on the expression of FGF-1 and SDF-1 genes in rats with second-degree burns.Materials and methods:  This experimental study used a second-degree burn model in Wistar rats, treated with subcutaneous injections of E-MSCs at doses of 100 µL and 200 µL. Gene expression of FGF-1 and SDF-1 was quantified using qRT-PCR. Histological validation confirmed burn severity, and flow cytometry was used to characterize E-MSCs and exosomes.Results: An increase in FGF-1 and SDF-1 expression was observed in exosome-treated groups compared to the NaCL-treated group. The 200 µL E-MSCs-treated group showed the most significant enhancement in both growth factors, with statistically significant differences (p<0.05). These findings underline the efficacy of E-MSCs in modulating critical genes involved in wound healing.Conclusion: E-MSCs significantly upregulate FGF-1 and SDF-1 expression, promoting tissue repair and regeneration in second-degree burn models. This study highlights the potential of E-MSCs as a non-invasive therapeutic approach.  Keywords: exosomes, FGF-1, mesenchymal stem cells, SDF-1
Protective Effect of Black Rice Extract Cream on Ultraviolet B-Induced Skin Hyperpigmentation in Mice Maryanti, Maryanti; Putra, Agung; Subchan, Prasetyowati
Althea Medical Journal Vol 12, No 3 (2025)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v12n3.4192

Abstract

Background: Hyperpigmentation is a common sign of skin aging caused by prolonged ultraviolet B (UVB) exposure. Black rice (Oryza sativa L. var glutinosa), known for its high antioxidant content, has moisturizing and regenerative properties that may support skin health. This  study aimed to evaluate the effect of black rice extract cream on transforming growth factor beta (TGF-β) and tumor necrosis factor alpha (TNF-α) expression in a UVB-induced hyperpigmentation mouse model.Method: An in vivo experimental study with post test only control group design was conducted in 2024 at the Stem Cell and Cancer Research Laboratory, Semarang, Indonesia. Twenty-eight male C57BL/6 mice were randomly divided into four groups: healthy control (K1),  UVB-exposed negative control group (K2), UVB-exposed group treated with  7.5% (K3) and 15 % (K4) black rice extract cream  for14 days. On day 15, TGF-β and TNF-α expression levels were analyzed using the RTq-PCR,  normalized to GAPDH. Data were analyzed using One-way ANOVA followed by post-hoc testing.Results: TGF-β gene expression was the highest in K4 (1.87±0.23), followed by K3 (1.52±0.42l) which was statistically significant different between groups (p=000); whereas TNF-α gene expression was the lowest in K4 (1.92±1.02) compared with K3 (5.40±2.28), and the difference between groups was also statistically significant (p=000).  Conclusion: Black rice extract cream increase TGF-β expression and reduces TNF-α expression in UVB-induced hyperpigmentation. These findings suggests its potential as a natural topical agent to mitigate UVB-induced skin damage and premature aging.
Co-Authors Adityani, Resanti Agung Putra Alif, Iffan Arlinda, Dyken Dwi Aryoko Widodo Berlian, Mukti Arja Eko Setiawan Handoyo, Frigi Eko Hariani, Nova Putri Husain, Sofian Azalia Husni Ahmad Sidiq Hussana, Atina Maryanti Maryanti Mayrona, Cindy Tia Soetrisno, Kusumawati