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All Journal International Journal of Cell and Biomedical Science
Handoyo, Frigi Eko
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Topical Gel of Mesenchymal Stem Cell-Conditioned Medium-induced Serum Injury Accelerates Wound Healing in Skin Excision Tissue Berlian, Mukti Arja; Alif, Iffan; Subchan, Prasetyowati; Handoyo, Frigi Eko; Husain, Sofian Azalia; Husni Ahmad Sidiq; Arlinda, Dyken Dwi; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.12

Abstract

Introduction: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) accelerating wound closure by increasing VEGF and PDGF level leading to re-epithelialization, cell infiltration, and angiogenesis. It has been found that MSC-conditioned medium (MSC-CM) can enhance migration of fibroblasts in scratch assays. However, the effect of MSC-CM-induced serum injury (MSC-CM-S) formulated in gel to accelerate wound healing remains unclear. This study aims to evaluate the effect of several doses of topical gel of MSC-CM-S in accelerating wound healing. Methods: The MSCs were cultured medium-supplemented serum injury of wounded rat (8:1) to get MSC-CM-S. The topical gel of MSC-CM-S was made by base gel supplemented with MSC-CM-S. Eighteen Wistar rats were randomly assigned into control (C) and treatment groups (T1, T2). Groups were received serum-free medium gel (C), 25 µl MSC-CM-S in topical gel (T1), 50 µl MSC-CM-S in topical gel (T2), twice daily for 9 days. PDGF and VEGF level and fibroblast density were measured by ELISA and HE staining at day 3 and 6, respectively. Results: This study showed that there was significant increase of VEGF and PDGF level along with a significant increase of fibroblast density at day 3 and 6. The T2 showed optimum enhancement level of VEGF, PDGF and fibroblast density. Conclusion: Topical gel of MSC-CM-S was effective to accelerate wound closure by enhancing PDGF and VEGF level in full-thickness skin defect rats.
Secretome Hypoxia-Mesenchymal Stem Cells Regulate IL-10 Concentrations in STZ-induced Type 1 Diabetes Rats Sutrisman, Intan Permatasari; Antari, Arini Dewi; Putra, Agung; Irawan, Risky Chandra; Handoyo, Frigi Eko
International Journal of Cell and Biomedical Science Vol 1 No 2 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i2.18

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Background: Type 1 Diabetic Mellitus (T1DM) is a well-known autoimmune disease that is characterized by a specific adaptative immunity against β-cell antigens. Mesenchymal stem cells (MSC) have emerged as potential immunomodulators in a paracrine manner via their bioactive soluble molecules that involve inflammation-related diseases, including T1DM. Objective: This study aims to investigate the effect of S-HMSC on regulating IL-10 concentrations in STZ-induced T1DM rats. Materials and Methods: This study uses a post-only control group design and randomized system. To induce T1DM rats, an intraperitoneal injection (65 mg/kg BW) of streptozotocin (STZ) was inducted. 20 Wistar rats were subdivided into the following groups: T1DM, DM with 0,5cc S-HMSC (Low-dose), and DM with 1cc S-HMSC (High-dose). The animals received an intraperitoneal injection of S-HMSC once a week for up to 4 weeks. On day 28, the animals were terminated and IL-10 concentrations were measured by ELISA. Results: After S-HMSC administration, the concentration of IL-10 in the treated group was increased in either low-dose or high-dose groups compared with the T1DM group. Conclusion: Administration of secretome-hypoxia MSC may regulate IL-10 concentrations in STZ-induced Type 1 Diabetes Rats.
The Effect of Hypoxia on the Soluble Molecules of Human Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSCs) Widyatmoko, Agus; Alif, Iffan; Irawan, Risky Candra Satria; Handoyo, Frigi Eko; Sidiq, Husni Ahmad
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.24

Abstract

Background: Umbilical cord-derived stem cells (UC-MSCs) are essential cell sources for cell-based therapies in regenerative medicine. Hypoxia is a key element of the stem cell niche and crucial for the preservation of UC-MSCs properties. The normal growth conditions for UC-MSCs are under atmospheric oxygen concentrations of 20–21%. However, the administration of UC-MSCs in inflammatory conditions provides oxygen-deficient environments. Thus, treating UC-MSCs with low oxygen exposure provides them with more survival and recovery potential. Objective: In this study, we assessed the impact of hypoxia incubation for 12 h on the UC-MSCs proteome. Methods: UC-MSCs were isolated from UC patients regarding informed consent. At passage 5, in 80% confluent, UC-MSCs were incubated in 5% O2 for 12 h. The morphology of UC-MSCs was assessed using a microscope. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were analyzed using ELISA. Results: Hypoxic condition could change their morphology and enhance the cellular density compared to normoxic conditions in vitro. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were significantly increased after the hypoxic condition of UC-MSCs compared to normoxia. Conclusion: Our findings suggest that the hypoxic condition was able to induce survival capacity and soluble molecules secreted by UC-MSCs.
Co-Authors Adityani, Resanti Agung Putra Agus Widyatmoko, Agus Alif, Iffan Antari, Arini Dewi Arlinda, Dyken Dwi Berlian, Mukti Arja Husain, Sofian Azalia Husni Ahmad Sidiq Irawan, Risky Candra Satria Irawan, Risky Chandra Prasetyowati Subchan, Prasetyowati Sidiq, Husni Ahmad Sutrisman, Intan Permatasari