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All Journal International Journal of Cell and Biomedical Science
Husain, Sofian Azalia
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Topical Gel of Mesenchymal Stem Cell-Conditioned Medium-induced Serum Injury Accelerates Wound Healing in Skin Excision Tissue Berlian, Mukti Arja; Alif, Iffan; Subchan, Prasetyowati; Handoyo, Frigi Eko; Husain, Sofian Azalia; Husni Ahmad Sidiq; Arlinda, Dyken Dwi; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.12

Abstract

Introduction: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) accelerating wound closure by increasing VEGF and PDGF level leading to re-epithelialization, cell infiltration, and angiogenesis. It has been found that MSC-conditioned medium (MSC-CM) can enhance migration of fibroblasts in scratch assays. However, the effect of MSC-CM-induced serum injury (MSC-CM-S) formulated in gel to accelerate wound healing remains unclear. This study aims to evaluate the effect of several doses of topical gel of MSC-CM-S in accelerating wound healing. Methods: The MSCs were cultured medium-supplemented serum injury of wounded rat (8:1) to get MSC-CM-S. The topical gel of MSC-CM-S was made by base gel supplemented with MSC-CM-S. Eighteen Wistar rats were randomly assigned into control (C) and treatment groups (T1, T2). Groups were received serum-free medium gel (C), 25 µl MSC-CM-S in topical gel (T1), 50 µl MSC-CM-S in topical gel (T2), twice daily for 9 days. PDGF and VEGF level and fibroblast density were measured by ELISA and HE staining at day 3 and 6, respectively. Results: This study showed that there was significant increase of VEGF and PDGF level along with a significant increase of fibroblast density at day 3 and 6. The T2 showed optimum enhancement level of VEGF, PDGF and fibroblast density. Conclusion: Topical gel of MSC-CM-S was effective to accelerate wound closure by enhancing PDGF and VEGF level in full-thickness skin defect rats.
Regulatory Effect of Secretome-Hypoxia Preconditioned Mesenchymal Stem Cells on TNF-α Level in Streptozotocin-induced Rats Amellia, Nazzala Luthfin; Antari, Arini Dewi; Nazar, Mohammad Ariq; Husain, Sofian Azalia; Arifin, Naufal Ardjivani
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.23

Abstract

Background: Type 1 Diabetic Mellitus (T1DM) is a well-known autoimmune disease characterized by a specific adaptative immunity against β-cell antigens. Mesenchymal stem cells (MSC) have emerged as potential immunomodulators in a paracrine manner via their bioactive soluble molecules that involve inflammation-related diseases, including T1DM. Objective: This study aims to investigate the effect of SHMSCs on regulating TNF-α concentrations in STZ-induced rats. Materials and Methods: This study uses a post-only control group design and randomized system that was conducted from December 2022 until January 2023. To induce T1DM-like rats, an intraperitoneal injection (65 mg/kg BW) of streptozotocin (STZ) was inducted. 15 male Wistar rats were subdivided into the following groups: STZ, STZ with 0.5 cc SHMSCs (Low-dose), and STZ with 1 cc SHMSCs (High-dose). The animals received an intraperitoneal injection of SHMSCs once a week for up to 4 weeks. On day 28, the animals were terminated and ELISA measured TNF-α concentrations. Results: After SHMSCs administration, the level of TNF-α in the treated group was decreased in either low-dose or high-dose groups compared with the STZ group. Conclusion: Administration of secretome-hypoxia MSCs may regulate TNF-α concentrations in STZ-induced Rats.
Clitoria ternatea Flower Extract Inhibits Matrix Mettaloproteinase-3 leading to the promotion of α-SMA Gene Expression in UVB-Induced Rat Model Muna, Nugraheni Itsnal; Amalina, Nur Dina; Husain, Sofian Azalia
International Journal of Cell and Biomedical Science Vol 2 No 5 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i5.33

Abstract

This study aimed to determine the effect of administration of Clitorea ternatea flower extract (C. ternatea) in a gel on the expression of MMP-3 and α-SMA in the skin of rat models induced by UVB light exposure. This research design is experimental research with a post-test control group design. This study was divided into four treatment groups: a healthy group, a UVB-negative control, a 5% C. ternatea gel treatment group (T1), and a 10% C. ternatea gel treatment group (T2). Negative control, T1, and T2 control groups were given UVB treatment with 302 nm energy of 160 mJ/cm2 for 5 days. In the treatment groups T1 and T2 were given the gel of C. ternatea from the 6th day until the 14th day. On day 15, all treatment groups were terminated, and the expression of MMP-3 and α-SMA genes was analyzed using qRT-PCR. In the 10% C. ternatea gel treatment group, there was a decrease in MMP-3 gene expression (0.73±0.26) and an increase in α-SMA gene expression (16.4±8.3) compared to the UVB control group which had gene expression. MMP-3 (3.24+1.05) and α-SMA gene expression (0.1±0.0). The administration of C. ternatea gel resulted in a reduction in the expression of the MMP-3 gene and an increase in the expression of the α-SMA gene. Overall, C. ternatea has the potential to prevent UVB-induced hyperpigmentation.
Secretome MSCs restore α-Smooth Muscle Actin Protein Tissue Expression in Croton Oil-Induced Hemorrhoid Rats Kusumo, M. Hidayat Budi; Husain, Sofian Azalia; Amalina, Nur Dina
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

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Abstract

Background: Hemorrhoidal disease, a prevalent and distressing condition affecting a significant number of the population, presents a considerable challenge in both clinical management and patient quality of life. Secretome mesenchymal stem cell hypoxia (S-HMSCs) is involved in accelerated remodeling and regeneration of wound tissue, including hemorrhoids, through anti-inflammatory and anti-fibrotic molecules paracrine activities. Objective: This study aims to investigate the effect of Secretome Hypoxia MSCs (S-HMSCs) in restoring α-smooth muscle actin (α-SMA) expression in croton oil-induced hemorrhoid rats. Material and Methods: An experimental study with a post-test-only control group design was used in this study. Croton oil was administrated for inducting hemorrhoidal disease. A total of 24 male Wistar rats were divided into four groups (n=6); Sham (Healthy group); Untreated (Croton oil+NaCl 300 µL) Croton oil+S-HMSCs 150); Secretome 150 µL (Croton oil+S-HM SCs 150 µL) and Secretome 300 µL (Croton oil+S-HMSCs 300 µL). S-HMSCs were injected intraperitoneally every week for up to 4 weeks. All animals were scarified and the rectal tissue was collected for α-SMA immunohistochemical staining analysis. Results: After hemorrhoid induction, α-SMA was expressed 20% higher than Sham group, furthermore, administration of 150 µL and 300 µL of S-HMSCs may decreased by 15% and 20% α-SMA expression compared to the Untreated group, expression in croton oil-induced hemorrhoid rats.
Time-Dependent Simulation Identifies Critical Hour Phase of Intestinal Acute Injury in Sepsis Mouse Model Istanti, Yusrina; Anggoro, Naufal Sebastian; Husain, Sofian Azalia; Azzahara, Salma Yasmine
International Journal of Cell and Biomedical Science Vol 3 No 9 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i9.64

Abstract

Background: Understanding the dynamic process of intestinal injury and repair during sepsis is essential for identifying optimal therapeutic windows. This study aimed to determine the critical time phase of intestinal acute injury by analyzing histological changes over a 24-hour period in a sepsis mouse model. Methods: Mice were divided into four groups—Control, 9 h, 12 h, and 24 h—and intestinal tissue samples were assessed using the Chiu histological scoring system. A time-dependent simulation was conducted to evaluate average changes in tissue damage and to identify key transition points between injury and recovery phases. Statistical analysis was performed using one-way ANOVA followed by post hoc comparisons to determine significant differences among time points. Results: The simulation demonstrated a marked increase in intestinal damage between 9 h and 12 h, followed by partial recovery at 24 h. Statistical analysis revealed a significant difference (p < 0.05) corresponding to this shift. These findings suggest that peak tissue injury occurs around 12 hours post-sepsis induction, preceding the onset of repair mechanisms. Conclusion: The study provides quantitative insight into the temporal progression of intestinal injury in sepsis, identifying the 12–24 hour period as a critical therapeutic window for potential interventions.
Co-Authors Adityani, Resanti Alif, Iffan Amalina, Nur Dina Amellia, Nazzala Luthfin Anggoro, Naufal Sebastian Antari, Arini Dewi Arifin, Naufal Ardjivani Arlinda, Dyken Dwi Azzahara, Salma Yasmine Berlian, Mukti Arja Handoyo, Frigi Eko Husni Ahmad Sidiq Kusumo, M. Hidayat Budi Mohammad Ariq Nazar Muna, Nugraheni Itsnal Prasetyowati Subchan, Prasetyowati Yusrina Istanti