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All Journal The Journal of Experimental Life Sciences (JELS) The Journal of Pure and Applied Chemistry Research
Mufidatur Rosyidah, Mufidatur
Master Program of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya,
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Effects of Low Temperature on Somatic Embryos Growth, Maturation and Planlet Regeneration of Citrus Mandarin var Batu 55 (Citrus reticulata Blanco.) Rosyidah, Mufidatur; Widoretno, Wahyu; Indriyani, Serafinah
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1011.479 KB) | DOI: 10.21776/ub.jels.2015.005.01.02

Abstract

This study was aimed to determine the effect of incubation at 4°C temperature during multiplication stage of somatic embryos on growth, maturation and plantlets regeneration of citrus Mandarin var Batu 55. Globular somatic embryos were cultured on MT (Murashige & Tucker) medium and incubated at 4°C temperature for 0, 2, 4, 6, 8 weeks. Maturation of somatic embryos was induced by transfering globular somatic embryos on maturation medium (MT + 50 mgl-1 malt extract + 73 mM sorbitol + 73 mM galactose). Cotyledonary embryo was regenerated on MT medium + 50 mgl-1 malt extract + 30 gL-1 sucrose + 2 ppm GA3. The research showed that maturation of somatic embyos at 4°C temperature inhibited growth and maturation of somatic embyos. Fresh weight of somatic embryo incubated at 4°C for 2 weeks lower than fresh weight of embryo cultured without incubation at 4°C, and continuous decline in longer incubation period. Maturation percentage of embryos without incubation at 4°C temperature was 15%, but embryos incubated at 4°C temperature were lower than 9%. There was no effect of incubation at 4°C temperature during somatic embryos multiplication stage on plantlet regeneration percentage. Keywords: Citrus reticulata, maturation, plantlet regeneration, somatic embryo
In silico Study of Bioactivity and Potential of Small Molecules from Manalagi Apple (Malus sylvestris L. Mill.) as Inhibitors of Trimethylamine-N-Oxide (TMAO) Precursors Karimatul Himmah; Putri Elok Septiana Dewi; Setiya Purbasari; Rosyidah, Mufidatur; Tiwuk Susantiningsih
The Journal of Pure and Applied Chemistry Research Vol. 15 No. 2 (2026): Edition May-August 2026
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2026.015.02.7965

Abstract

Targeting the precursors of trimethylamine-N-oxide (TMAO), such as choline, betaine, and enzymes produced by the FMO3 gene, through natural compounds may reduce risk factors such as cardiovascular and metabolic diseases. Protein precursors involved in high TMAO level production include cntA, CUTC, and FMO3. Manalagi apple (Malus sylvestris) is rich in bioactive compounds, including pectin, phloretin, and chlorogenic acid, with potential health benefits, and would affect the decreasing production of TMAO by inhibiting some protein precursors. This research aims to investigate the potential of three small molecules in it as a dietary source candidate and drug candidate that could mitigate the risk of TMAO-related diseases based on their interaction and binding affinity through an in-silico study. The methods include the prediction of protein and small-molecule structures, including FTIR, molecular docking, and analysis of small-molecule bioavailability. Pectin is considered the top candidate compared to phloretin and chlorogenic acid based on its high binding affinity to interact with CUTC, cntA, and FMO3 (-10.2, -8.6, and -9.1 kcal/mol, respectively). Phloretin is a promising compound for drug development based on its binding site to interact with more residues and bonds, its bioavailability with a drug likeness score of 0.55, and high gastrointestinal absorption.
Co-Authors Karimatul Himmah Putri Elok Septiana Dewi Serafinah Indriyani Setiya Purbasari Tiwuk Susantiningsih Wahyu Widoretno