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All Journal The Journal of Experimental Life Sciences (JELS) Biotropika AGRIVITA, Journal of Agricultural Science
Wahyu Widoretno
Dept. of Biology, University of Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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DROUGHT RESISTANT SELECTION ON SOYBEAN SOMACLONAL VARIANTS Widoretno, Wahyu; Arumingtyas, Estri Laras; Basuki, Nur; Soegianto, Andy
AGRIVITA, Journal of Agricultural Science Vol 34, No 1 (2012)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

This research was conducted to evaluate the yield potential of 19 somaclonal variants resulting from in vitro selection when planted under drought stress condition in the field. Field test was done by planting the variants, the parents, and checked varieties in the field during dry season, and was irrigated once a week for non-stress and once two weeks for drought stress treatment. Split-plot design arranged in a factorial (2 x 28) with three replications was used in this research. Observations were done on yield and yield components. Analysis of variance was used to see the difference between treatments and then it was continued with analysis using Honestly Significant Difference test to find out the best treatments. There was no interaction between genotype and drought stress on seed yield. Different genotypes showed a significant difference on this character. It indicated that the yield potential of selected variants was not affected by drought stress treatment. This research gave 10 variants having the potential to be developed as drought resistant genotypes. However, these ten potential genotypes need to be tested further in field trial to find out the yield adaptability and stability and their resistance to drought stress.   Keywords: somaclonal variants, in vitro selection, drought stress, soybean, selection
Regenerasi In Vitro Tanaman Bawang Merah (Allium ascalonicum L.) Kurniawan, Alfian Dwi; Widoretno, Wahyu
Biotropika: Journal of Tropical Biology Vol 4, No 1 (2016)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh zat pengatur tumbuh yang ditambahkan pada media terhadap induksi kalus dan regenerasi tanaman bawang merah (Allium ascalonicum L.) secara in vitro. Kalus diinduksi dari eksplan cakram /basal stem menggunakan media Murashige dan Skoog (MS) dengan penambahan 2,4-D konsentrasi 1, 2, 3 dan 4 mg/L. Kalus dikultur pada media MS dengan penambahan NAA 0,1 mg/L dan beberapa konsentrasi  kinetin (2, 3 dan 5 mg/L) untuk induksi tunas. Tunas disubkultur pada media MS + IBA 2 mg/L untuk membentuk plantlet. Penambahan 2,4-D pada media kultur mampu menginduksi pembentukan kalus pada eksplan, tetapi konsentrasi 2,4-D yang tinggi pada media menghambat pembentukan dan pertumbuhan kalus serta menyebabkan terjadi pencoklatan pada eksplan. Penambahan kinetin yang dikombinasikan dengan NAA pada medium mampu menginduksi tunas bawang merah dari eksplan kalus. Peningkatan konsentrasi kinetin berpengaruh terhadap jumlah tunas yang terbentuk pada kalus. Konsentrasi kinetin 2 mg/L mampu menghasilkan tunas lebih banyak dibandingkan dengan kinetin pada konsentrasi yang lebih tinggi (3 dan 5 mg/L). Plantlet bawang merah diperoleh setelah tunas disubkultur pada media yang mengandung IBA 2mg/L, akar mulai muncul setelah 6-8 minggu kultur.
Regenerasi Tanaman dari Eksplan Kalus Bawang Putih (Allium sativum L.) secara In Vitro Fauziah, Arbaul; Widoretno, Wahyu
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh zat pengatur tumbuh auksin dan sitokinin terhadap induksi kalus dan regenerasi tanaman bawang putih (Allium sativum L.) secara in vitro. Medium Murashige and Skoog (MS) dengan penambahan kombinasi 2,4-D dan kinetin digunakan untuk induksi kalus dari eksplan meristem basal,  sedangkan NAA dan BAP digunakan untuk regenerasi planlet dari eksplan kalus. Pertumbuhan kalus pada medium kultur yang mengandung 2,4-D dan kinetin pada konsentrasi rendah lebih baik daripada konsentrasi tinggi. Kalus yang dikulturkan pada medium kultur yang mengandung NAA 0.1 ppm dikombinasikan dengan BAP 0.5-2 ppm mampu membentuk tunas. Jumlah tunas meningkat seiring dengan peningkatan konsentrai BAP. Di antara berbagai macam konsentrasi NAA dan BAP, jumlah tunas terbanyak diperoleh pada medium kultur yang mengandung NAA 0.1 ppm dan BAP 2 ppm. Tunas-tunas dipindahkan ke medium kultur dengan penambahan BAP 10 ppm untuk menginduksi pemanjangan tunas. Planlet bawang putih dihasilkan dari tunas yang dikulturkan pada medium kultur dengan penambahan IBA 2 ppm. Kata kunci: bawang putih, kalus, meristem basal, regenerasi in vitro
DROUGHT RESISTANT SELECTION ON SOYBEAN SOMACLONAL VARIANTS Widoretno, Wahyu; Arumingtyas, Estri Laras; Basuki, Nur; Soegianto, Andy
AGRIVITA, Journal of Agricultural Science Vol 34, No 1 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i1.136

Abstract

This research was conducted to evaluate the yield potential of 19 somaclonal variants resulting from in vitro selection when planted under drought stress condition in the field. Field test was done by planting the variants, the parents, and checked varieties in the field during dry season, and was irrigated once a week for non-stress and once two weeks for drought stress treatment. Split-plot design arranged in a factorial (2 x 28) with three replications was used in this research. Observations were done on yield and yield components. Analysis of variance was used to see the difference between treatments and then it was continued with analysis using Honestly Significant Difference test to find out the best treatments. There was no interaction between genotype and drought stress on seed yield. Different genotypes showed a significant difference on this character. It indicated that the yield potential of selected variants was not affected by drought stress treatment. This research gave 10 variants having the potential to be developed as drought resistant genotypes. However, these ten potential genotypes need to be tested further in field trial to find out the yield adaptability and stability and their resistance to drought stress.   Keywords: somaclonal variants, in vitro selection, drought stress, soybean, selection
Effect of Ethyl Methane Sulfonate on Bulbil Explant Growth and In Vitro Shoot Formation in Porang (Amorphophallus muelleri Blume) Febriyanti, Vera; Waluyo, Budi; Harijati, Nunung; Martasari, Chaireni; Widoretno, Wahyu
The Journal of Experimental Life Science Vol. 14 No. 2 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.02.04

Abstract

Porang is one of the plant commodities with high economic value due to its high glucomannan content in the tubers. Currently, the global demand for porang tubers continues to rise, but domestic production has not yet met this demand due to the limited supply of superior porang seeds. Mutation breeding with Ethyl Methane Sulfonate (EMS) can be one of the alternative methods for developing superior porang to enhance tuber production. This study aims to determine the response of porang bulbil explants to EMS mutagen treatment in vitro. The research used a Randomized Complete Block Design consisting of six EMS concentrations (0, 0.02, 0.04, 0.06, 0.08, and 0.1%). The research stages included pre-culture of bulbil explants on MS + BAP 3 mg.L-1 + NAA 0.1 mg.L-1 for two weeks in dark conditions and EMS mutagen treatment on bulbil cultures for four weeks. The results showed that adding EMS mutagen to the medium for four weeks caused the explants browning, inhibited shoots forming, and decreased the fresh weight and growth index. The higher the EMS concentration in the medium, the increased percentage of browning explants and reduced explant growth and shoot formation. The EMS concentration (≥0.06%) inhibited explant growth and shoot formation. While EMS concentration (0.02-0.04%) increased explant growth, shoot formation was not significantly different from control, even though there was an increase. Up to four weeks of culture, there was no explant death, so LD50 is not yet known. The LD50 EMS can also be calculated by explant browning and shoot proliferation. Based on explant browning and shoot proliferation, the LD50 EMS in porang bulbil explants was 0.07% and 0.09%. The EMS concentration and length of culture age need to be increased to obtain an LD50 survival rate. Keywords: Amorphophallus muelleri Blume, bulbil, EMS, In Vitro mutagenesis.
The The Effect of Gamma Irradiation on In Vitro Explant Growth, Shoot Multiplication, and Plantlet Regeneration Explants Bulbil of Porang (Amorphophallus muelleri Blume) Ni'amul Albab, Muhammad; Widoretno, Wahyu; Siswanto, Dian; Waluyo, Budi; Mertasari, Chaerani
The Journal of Experimental Life Science Vol. 15 No. 1 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.01.06

Abstract

Porang (Amorphophallus muelleri Blume) is a tuberous plant that contains glucomannan as an alternative in reducing obesity. It is widely traded, but it is cultivated through conventional methods and takes a long time. In vitro mutagenesis technique with gamma irradiation is expected to increase the production of superior porang. This study aims to determine the effect of gamma irradiation on explant growth, shoot multiplication, and plantlet regeneration on in vitro culture. The doses of gamma irradiation, i.e. 0, 2, 4, 6, 8, and 10 gamma rays (Gy). Explants bulbil were precultured for 14 days on Murashige and Skoog (MS) medium fortified with 6-benzyl amino purine (BAP) 3 mg.L-1 before irradiation. The irradiated explants were then cultured on media containing MS supplemented with α-naphthalene acetic acid (NAA) 0.1 mg.L-1 and BAP 3 mg.L-1 for 4 and 8 weeks of culture. Plantlet regeneration media was MS supplemented with NAA 0.1 mg.L-1 for 4 weeks of culture. The results showed that a dose of 10 Gy gamma irradiation was able to inhibit explant growth (0.06 g), shoot multiplication (0%), and plantlet height (0.38 cm), while gamma irradiation at a dose of 6 Gy increased explant growth (0.16 g), shoot multiplication (40%), and plantlet height (5.60 cm). The lethal dose 50 (LD50) of gamma irradiation for explant survival rate is 8.79 Gy. Keywords: Amorphophallus muelleri, explant growth, gamma irradiation, in vitro, plantlet regeneration, shoot multiplication
Effect of Pb and Cd Elicitors on Growth and Content of Vetiver Oil Adventitious Root In Vitro of Vetiver (Vetiveria zizanioides L. Nash) Ivani, Trecy; Widoretno, Wahyu
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1113.175 KB) | DOI: 10.21776/ub.jels.2019.009.03.09

Abstract

This research aimed to evaluate the effect of Pb and Cd elicitors on root growth and essential oil content of vetiver oil on the root culture of vetiver (Vetiveria zizanioides L. Nash). Roots were induced by culturing of the shoot on MS media supplemented with 0.1 mg.L-1 kinetin and 7 mg.L-1 NAA. Elicitation was done by culturing roots on MS media with 0.1 mg.L-1 kinetin and 7 mg.L-1 NAA + 0.1 mM Pb or Cd. Roots then observed for fresh weight, dry weight, number, and root length, and vetiver oil contents were analyzed using GC-MS. The addition of Pb and Cd heavy metal elicitor affected the formation and the content of vetiver oil compounds of root culture. Pb elicitor increased the number of roots, while Cd elicitor increased root length. However, the addition of Pb and Cd elicitor on culture media decreased the fresh weight and root dry weight. Fifty eight vetiver oil compounds in adventitious roots of vetiver plants were identified. The main compounds of vetiver oil in roots culture were Khusimone (6.94%), Khusimol (6.05%), Khusimene (4.85%), α Vetivone (3.70%), β Vetivone (3, 53%), Vetiverol (3.22%), Prezizaene (2.35%), and Zizaene (1.91%). Elicitation with Pb and Cd increased the composition of the main compounds of vetiver oil. Cd elicitor increased the composition of the main compound vetiver oil higher than the Pb elicitor. Keywords: adventives root, elicitor, in vitro, Pb and Cd, vetiver oil
Co-Authors Alfian Dwi Kurniawan, Alfian Dwi Andy Soegianto Andy Soegianto Arbaul Fauziah Budi Waluyo Chaireni Martasari Dian Siswanto Estri Laras Arumingtyas Febriyanti, Vera Ivani, Trecy Mertasari, Chaerani Ni'amul Albab, Muhammad Nunung Harijati Nur Basuki Nur Basuki