Hans Kristian Nugraha, Hans Kristian
Medical Faculty Universitas Airlangga Surabaya hans.nugraha@yahoo.com, +62315940696, +62315927764

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PLATELET RICH PLASMA PREPARATION PROTOCOLS: A PRELIMINARY STUDY Nugraha, Hans Kristian; Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.43 KB)

Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable methods according to the literature is a double centrifugation method. The purpose of this research is to produce anoptimization of the double centrifugation method. This study used experimental data obtained by conducting a research at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments were conducted on stored blood obtained from the blood bag from Indonesian Red Crossand fresh blood from healthy donors with CPD anticoagulant. Results: PRP with optimum platelet count could be made with sufficient personal laboratory skills and amounted to 4.11 times with the platelet count of 1.152 million using 1300 rcf for 5 minutes for the first centrifugation, and 2300 rcf for 7 minutes for the second centrifugation.
PLATELET RICH PLASMA PREPARATION PROTOCOLS: A PRELIMINARY STUDY Nugraha, Hans Kristian; Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 2 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.43 KB) | DOI: 10.20473/ijtid.v3i2.216

Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable methods according to the literature is a double centrifugation method. The purpose of this research is to produce anoptimization of the double centrifugation method. This study used experimental data obtained by conducting a research at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments were conducted on stored blood obtained from the blood bag from Indonesian Red Crossand fresh blood from healthy donors with CPD anticoagulant. Results: PRP with optimum platelet count could be made with sufficient personal laboratory skills and amounted to 4.11 times with the platelet count of 1.152 million using 1300 rcf for 5 minutes for the first centrifugation, and 2300 rcf for 7 minutes for the second centrifugation.
Comparison of Mortality Rate Between Bipolar Hemiarthroplasty and Proximal Femoral Nail Anti-rotation for Intertrochanteric Fractures in Sanglah Hospital, Bali Dusak, I Wayan Suryanto; Aryana, I Gusti Ngurah Wien; Dharmayuda, Cokorda Gde Oka; Subawa, I Wayan; Nugraha, Hans Kristian; Susila, Made Arya; Mahadhana, Sri
The Hip and Knee Journal Vol 1, No 1 (2020): August
Publisher : Indonesian Hip and Knee Society (IHKS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (844.577 KB) | DOI: 10.46355/hipknee.v1i1.25

Abstract

Introduction: Intertrochanteric fractures occur in about 50% of all hip fracture events, with a mortality rate within 1 year after fracture reaching 15 to 20%. The most common treatment nowadays is either the bipolar hemiarthroplasty procedure or proximal femoral nail anti-rotation (PFNA), although there is still no consensus regarding which is better from the two, especially on patient mortality.Method: This study was an observational study using a retrospective cohort design. A total of 102 study subjects who met the inclusion requirements were grouped into 2 groups, one with bipolar hemiarthroplasty fixation treatment and another with PFNA fixation treatment. Mortality rate was recorded by survey 2 years after surgery.Results: Chi-square test showed that 2-year mortality rate after intertrochanteric fracture treated with bipolar hemiarthroplasty (21.4%) was significantly higher than the PFNA group (10.3%) (p = 0.028). Bipolar hemiarthroplasty group also had longer length of stay (50%) than the PFNA group (32.4%), albeit statistically insignificant (p = 0.13). There was no significant difference between the 2-year mortality rate and length of stay (p = 0.976).Conclusion: Patients with intertrochanteric fractures who underwent bipolar hemiarthroplasty have significantly higher 2- year mortality rate than similar patients underwent fixation with PFNA, while they did not experience higher length of stay than the PFNA group. Future prospective, multi center study with larger sample size will be likely to validate similar fixation choice needed to decrease the mortality rate in intertrochanteric fractures.
OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS Nugraha, Hans Kristian; Hernaningsih, Yetti; Nugraha, Jusak
Folia Medica Indonesiana Vol. 51 No. 3 (2015): July - September 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.569 KB) | DOI: 10.20473/fmi.v51i3.2832

Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable according to the literature is a double centrifugation method. The purpose of this research is to produce an optimization of the double centrifugation method. This study uses experimental data obtained by researchers at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments generally were conducted on stored blood obtained from the blood bag from PMI with CPD anticoagulant. Optimization of double centrifugation method to produce PRP with optimum platelet count could be obtained by using an "I"-shaped tube as well as other specialized equipment which are currently not available in standard clinical pathology laboratory, and also fresh blood. The resulting increase amounted to 4.11 times with the platelet count of 1.152 million.
EPIDEMIOLOGY OF FRACTURES AND DISLOCATIONS IN CHILDREN Nugraha, Hans Kristian; Adiantono, Agus
Folia Medica Indonesiana Vol. 53 No. 1 (2017): JANUARY - MARCH 2017
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.88 KB) | DOI: 10.20473/fmi.v53i1.5494

Abstract

Fracture is quite a common occurrence in the age group of children, with a fairly wide variety of epidemiology throughout the world, hence it could be considered as a major health problem. A retrospective study of all pediatric fractures presenting to Dr. Soegiri General Hospital Lamongan, Indonesiain 2015 was undertaken. It showed that 79.5% of children's fractures occurred in males and that 86.76% presented as a single fracture, whileelbow dislocation is the most prevalent dislocation in this study.Analysis of pediatric fractures shows that there is a trimodal distribution of single fracture with age, withdistal radius/ulna fracture as the most prevalent single fracture.The commonest cause of single fracture are road traffic accident, and the majority involve the upper limb. Those suggested that there should be more concern about road safety program in Indonesia.