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KINERJA ENZIM GANDA PADA PRETREATMENT MIKROALGA UNTUK PRODUKSI BIOETANOL Padil, Padil; Syamsiah, Siti; Hidayat, Muslikhin; Kasiamdari, Rina Sri
Jurnal Bahan Alam Terbarukan Vol 5, No 2 (2016): December 2016 [Nationally Accredited]
Publisher : Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v5i2.7564

Abstract

The use of biomass of microalgae as a feedstock to produce bioethanol is very promising, it is caused by a large amount of carbohydrates contained in microalgae physiology cell. The main obstacle of enzymatic hydrolysis in order to produce bioethanol is the bound starch granules in a rigid cell wall. Therefore, pre-treatment steps needed to remove and convert complex carbohydrates into simple sugars before the fermentation process. Tetraselmis Chuii microalgae species are green microalgae (Chlorophyta) in which the cell wall containing cellulose and hemicellulose as the main constituent, therefore, this study observe the effect of the use of cellulase enzymes and xylanase as a strategy to open up the cell walls of microalgae. Another investigated parameter is the enzyme concentration, temperature, pH, and methods of use of enzymes. The results showed that the highest yield of glucose obtained was 31.912% (w / w) and is achieved under the conditions of a temperature of 45oC, pH of 4.5, the amount of biomass of microalgae as 5 g/L, the concentration of cellulase enzymes and xilanase 30% (w / w) at 40 minute at mechanism using cellulase and xylanase enzymes simultaneously.
Pembuatan Biodiesel dari Minyak Kelapa melalui Reaksi Metanolisis Menggunakan Katalis CaCO3 yang dipijarkan Padil, Padil; Wahyuningsih, Slamet; Awaluddin, Amir
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.372 KB) | DOI: 10.31258/jnat.13.1.27-32

Abstract

Biodiesel is one of alternative fuels. Biodiesel can be made from coconut oil and is referred to as cocodiesel. Thisresearch studies the optimum condition of cocodiesel production by methanolysis reaction between coconut oiland methanol using heterogen catalyst of calcium carbonate (CaCO 3) is calcined for 1.5 hours at 900oC. In order toget optimum condition, several experimental parameters are applied such as catalyst concentration 1-3 %-wt andmolar ratio of methanol/coconut oil 4:1-12:1. The optimum condition obtained from this experimental as follow:catalyst concentration was 2%, molar ratio of methanol/coconut oil was 8:1 at 600C, produced the higgest conversionof cocodiesel 75.02%. The cocodiesel meet the requirement of Standar Nasional Indonesia (SNI) specifications.Based on data analysis, the product has a qualification as diesel fuel. SNI Biodisel is density (40 0C) 850-890Kg/m3,kinematic viscosity (40 0C) 2,3-6,0 mm2/s, cetane number min 51, iod number max 115 gr iod/100 gram,moisture content max 0,05 % volum, acid number max 0,8 mg KOH/g, flash point min 100 0C.
CELL DISTRUPTION MIKROALGA SECARA ENZIMATIS DENGAN SELLULASE Padil Padil; Siti Syamsiah; Muslikhin Hidayat; Rina Sri Kasiamdari
Reaktor Volume 15 No.4 Oktober 2015
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.658 KB) | DOI: 10.14710/reaktor.15.4.213-217

Abstract

ENZYMATIC CELL DISRUPTION OF MICROALGAE USING CELLULASE. Micro-algae is one source of potential alternative energy of third generation to be developed as bioethanol raw material. The starch content trapped in Micro-algae cell walls causing the need of cell distruption to release and convert starch into simple glucose before the fermentation process. This study aims to open up the cell walls of Micro-algae and to explore the effect of sellulase enzymes from Aspergillus niger as Micro-algae cell distruption strategy as well as the optimization of process parameters, i.e the concentration of enzyme, temperature, pH, and time which produce the highest glucose yield. The results showed that the highest glucose yield was 82.44% (w/w) obtained at an enzyme concentration of 30% (w/w), temperature 45oC, pH of 4.5 at 40 minutes, the amount of Micro-algae as 0.5 g/L. In general, cell distruption method using sellulase enzyme was proven to be a promising option to open the cell walls of Micro-algae and convert cellulose into simple glucose simultaneously in producing bioethanol.   Keywords: bioethanol; cellulose; cell distruption; enzymatic; micro-algae; starch Abstrak Mikroalga merupakan salah satu sumber energi alternatif generasi ketiga yang potensial untuk dikembangkan sebagai bahan baku bioetanol. Kandungan pati yang terperangkap dalam dinding sel mikroalga menyebabkan perlunya cell distruption untuk melepaskan sekaligus mengkonversi pati menjadi glukosa sederhana sebelum proses fermentasi. Penelitian ini bertujuan untuk membuka dinding sel mikroalga sekaligus mengeksplorasi pengaruh enzim sellulase dari aspergillus niger sebagai strategi cell distruption mikroalga serta melakukan optimasi parameter proses yaitu konsentrasi enzim, suhu, pH, dan waktu yang memberikan yield glukosa tertinggi. Hasil penelitian menunjukkan bahwa yield glukosa tertinggi adalah 82,44% (w/w) yang diperoleh pada konsentrasi enzim 30% (w/w), suhu 450C, pH 4,5 pada waktu 40 menit, dengan jumlah mikroalga 0,5 g/L. Secara umum, metode cell distruption dengan menggunakan enzim sellulase terbukti menjadi pilihan yang menjanjikan untuk membuka dinding sel mikroalga sekaligus mengkonversi selulosa menjadi glukosa sederhana dalam memproduksi bioetanol. 
KARAKTERISTIK PERTANGGUNGJAWABAN PIDANA KORPORASI DALAM TINDAK PIDANA KORUPSI Padil Padil
Jurnal IUS Kajian Hukum dan Keadilan Vol 4, No 1 (2016): HUKUM DAN TANGGUNG JAWAB
Publisher : Fakultas Hukum Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.025 KB) | DOI: 10.12345/ius.v4i1.280

Abstract

Dalam sistem hukum di Indonesia, Korporasi adalah subjek hukum buatan yang dapat dikenakan pertanggungjawaban pidana sebagai subyek hukum (Rechtpersoon), korporasi bukan hanya diberi kewenangan untuk bertindak seperti individu, tetapi ditambah dengan kebebasan yang besar dalam menjalankan kegiatan ekonomi.Penelitian di lakukan dengan metode kuantitatif dan pendekatan normative dengan melihat ketentuan hukum pertanggungjawaban pidana korporasi di Indonesia. Dalam penelitian ditemukan bahwa hukum positif di Indonesia mengatur hal yang berkaitan dengan pemberantasan tindak pidana korupsi yang relevan dengan kasus-kasus tindak pidana korupsi yang dilakukan oleh korporasi, namun pada saat itu tidak mengatur pertanggungjawaban pidana korporasi, oleh karena itu semua kasus yang menggunakan proses peradilan pidana didasarkan pada pertanggungjawaban perorangan yang pada umumnya adalah para pengurus atau pemegang saham atau orang yang memegang peran penting dalam beroperasinya korporasi tersebut, Dengan demikian konstruksi penyidikan dan penuntutan perkara, didasarkan pada perbuatan individu dan tidak berorientasi pada pertanggungjawaban pidana korporasi itu sendiri, sehingga korporasi tidak di tuntut ataupun didakwa, padahal korporasi sudah dianggap mampu melakukan tindak pidana,  maka semestinya harus dianggap mampu bertanggung jawab sebagaimana pertanggungjawaban orang perorangan, sehingga terlihat penarapan Undang-undang Pemberantasan Tindak Pidana Korupsi yang pelakunya tidak saja orang namun juga korporasi, karena Undang-undang diluar KUHP mengakui manusia (naturlijk persoon) dan korporasi sebagai subjek delik. Kata Kunci   :  Karakteristik Pertanggungjawaban Pidana Korporasi Dalam Tindak Pidana Korupsi.
Pemurnian Selulosa Alfa Pelepah Sawit Menggunakan Enzim Xylanase Caesari Caesari; Padil Padil; Yelmida Yelmida
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Midrib of palm oil is the most solid wastes which produced in oil palm plantations. Palm midrib is contained of 34.89% α-cellulose content percentage that can be improved with cooking using the extract solution ash of palm empty fruit bunches (Padil, 2010) and continued with the process of purification using xylanase enzyme. Higher content of Cellulose-α can be processed into a more commercially product. Cellulose-α> 92% qualified to be used as the main raw material (nitrocellulose) or making explosives and propellants. Stages of the purification process a palm midrib cellulose is extraction, hydrolysis, delignification and purification using xylanase enzyme with variations process is temperature (50, 60 and 70 ° C), enzyme dose (1, 2 and 3 ml) and the source of the enzyme (Aspergillus and Trichoderma sp so). Purification process operating conditions are: pH 5, time 90 minutes, the solid-solution 1:25. Content of purified cellulose-α increased to 96.60% for variable temperature at 60 ° C, 3 ml dose of enzymes and enzyme xylanase from Aspergillus sp. Keywords : Cellulose Alpha, Palm Midrib, Xylanase Enzyme
Kultivasi Mikroalga Menggunakan Media Af6 Pada Berbagai PH Jelizanur Jelizanur; Padil Padil; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Microalgae cultivation is one technique to grow microalgae in a controlled environment. Cultivation aims to provide a single species in microalgae mass culture for harvesting. This research used AF6 media with a volume of 90 ml and 10 ml of microalgae. The purpose of this research was to cultivate microalgae using AF6 media with variations in pH 4, 6, 8 and types of microalgae namely Chlamydomonnas sp (chl), Chlamydomonnas sp (019), Chlamydomonnas sp (4), Chlamydomonnas sp (5), Chlorella sp (6 ). The results showed that pH had a significant effect on microalgae cultivation. Microalgae Chlorella sp (6) with a combination of treatment pH 8 and observation time of the 10th day was the best treatment of all types of microalgae cultivated that is equal to 0.1255.Keywords: microalgae, AF6 media, pH
Kultivasi Mikroalga Menggunakan Media AF6 Berdasarkan Perbedaan Intensitas Cahaya Nova Diyana Nurhanifah; Padil Padil; Sri Rezeki Muria
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Microalgae cultivation use AF6 media based on light intensity difference has done in this research. Light intensity has an important function in the process of photosynthesis. Optimization of light intensity is expected to increase growth microalgae. In the microalgaecultivation of laboratory scale used light lamp for substitute sunlight. This research purpose for determine the effect of light intensity differences on spesific growth rate on various microalgae cultivated in AF6 media. In this research observations of cell density microalgaechlorella sp. (6) and chlamydomonas sp. (4, 5, 19, chl) at media AF6 on the variation intensity 500 lux, 1.000 lux and 1.500 lux are did with testing the optical density of each microalgae with wavelength of 680 nm using UV-VIS spectrophotometer. The research resultexplain specific growth rate and shortest doubling time occurred in the light intensity 1.500 lux. Microalgae which has spesific growth rate and shortest doubling time are microalgae Chlorella sp.with value 0,78/day, and 0,88 day respectively.Keywords: light intensity, density, growth, microalgae
Pemurnian Selulosa-α Hasil Hidrolisis Pelepah Sawit Menggunakan Enzim Xylanase Dengan Variasi pH Dan Sumber Enzim Xylanase Sari D. P; Padil Padil; Yelmida Yelmida
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Midrib palm is one of the waste that produced from palm farming which contain alpha cellulose about 35%. The component of alpha cellulose could be used as a raw material for nitrocellulose if it is has more than 92% of purity. So to increase the purity of alpha cellulose, midrib palm has to be treated by purifying step. In this time, chemical still be choices to be material for purify alpha cellulose, while as we know that chemical has a bad impact for our envirotment. So it is important to search another purifying agent and it is xylanase. Xylanase is one of enzyme that has an ability to break up the link between xylose in xylan. So the purpose of this research are to use xylanase as another purifying agent, to know what is the best pH for this purifying process and to compare the result between two xylanases that produced from different fungi. Before start the purifying step, midrib have to through extraction step to remove all extractive and continue with hydrolisis step by using extraction liquid of palm empty bunch ash. Then after that purifying step can be done at 600C for 1,5 hours by making some differences at pH (4,5 and 6). The highest purity of alpha cellulose was reached at pH 6 by using xylanase from Aspergillus niger about 97,55%. So the conclucion of this research are, the best pH for purifying process by using xylanase is 6 and the best xylanase is the one which produce from Aspergillus niger. Beside that, the use of xylanase which is comes from the same species will be not affected much to the purifying result.Keyword : hydrolisis, midrib palm, xylanase enzyme
Pengaruh Waktu Reaksi Dan Penambahan Volume Enzim terhadap Pemurnian Selulosa-α Pelepah Sawit Menggunakan Enzim Xilanase Dari Trichoderma sp Putri M F; Padil Padil; Yelmida Yelmida
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Palm midrib is one of the solid waste from oil palm plantattions and there is approximately 35% α- cellulosa component. Cellulose which contained in waste palm midrib are very potential to transform into nitrocellulose raw material. Purify of cellulose above 92% can be used to make main propelan raw material or explosive matter. The purpose of this research was to got the best condition of addition from xylanase enzyme and the best time of reaction from purifying α-cellulose palm midrib process as a main nitrocellulose raw material. Palm midrib has to accomplished by several steps of purify such as extraction, hydrolysis, and delignyfication, and then continue to purifying process with xylanase enzyme on 60oc phase, pH 5, ratio of palm midrib: aquadest 1:25 in term of enzyme volume addition and time of reaction variation. The analyst of cellulosa that pass the hidrolysis show the purity α-cellulose is 86,48%. The best condition is able at  enzyme volume addition 3 ml and time of reaction 90 menit with the purity of α-celluloseobtained 96,6%. So it can be concluded that α-cellulose of the palm midrib pass purity process used the xylanase enzyme can be used for the manufacture of nitrocellulose.Keyword :α-cellulosa,nitrocellulose, palmmidrib,purification,xylanase enzyme
Pembuatan Nitroselulosa Dari Selulosa Hasil Pemurnian Pelepah Sawit dengan Hidrogen Peroksida (H2O2) Sebagai Bahan Baku Pembuatan Propelan Saragih E; Padil Padil; Yelmida Yelmida
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Nitrocellulose is a polymer used as materials for propellant. Manufacture of nitrocellulose done with cellulose nitration process. Nitration of cellulose is a reaction force substitution or replacement of H+ from –OH- groups in cellulose contained in the NO2+ cluster of nitric acid (HNO3). Cellulose to be used is sourced from waste palm midrib. The purpose of this study is to obtain the best conditions of temperature and time nitration that α- cellulose nitration process to produce nitrocellulose with a high nitrogen content. Palm midrib cellulose purified before entering the nitration process. Stages of the purification process a palm midrib cellulose is extraction, hydrolysis, delignification and purification with hydrogen peroxide (H2O2). After the purification process, the next step is the process of nitration of cellulose with a mixture of nitric acid and sulfuric acid. Nitration process is done with the variation of temperature and reaction time. Nitrocellulose nitrogen levels will be analyzed with Fourier Transform Infra Red Spectroscopy (FTIR). The best conditions in the nitration process took 30 minutes with the temperature of 90 oC. Nitrogen levels were estimated on nitrocellulose > 12% so it can be used as raw material for the manufacture of propellants. Keywords: FTIR, Nitration, Nitrocellulose, Palm Midrib, Propellants