Udi Tarwotjo, Udi
Department Of Biology, Faculty Of Science And Mathematics, Diponegoro University

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Keanekaragaman dan Kelimpahan Semut sebagai Predator Hama Tanaman Padi di Lahan Sawah Organik dan Anorganik Kecamatan Karanganom Kabupaten Klaten Adhi, Siska Lesiana; Hadi, Mochamad; Tarwotjo, Udi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.208 KB) | DOI: 10.14710/bioma.19.2.125-135

Abstract

Ants potentially as predators that become natural enemies of insect pests in rice fields with paddy cultivation. This study aims to know the diversity and abundance of ants, the role of ants, the population of ants, and the influence of physical and chemical factors in organic and inorganic rice fields. The ants were collected using pit fall trap method with insect bait, fish meat, and sugar solution. The results showed that the number of species and the number of individuals in organic rice fields was higher (429 individuals, 11 species) than inorganic rice fields (193 individuals, 10 species). In general, the index of diversity in organic rice fields was higher (ranging from 0.73 to 1.65) compared to inorganic rice fields (ranging from 0 to 1.28). The index of evenness in organic rice fields ranged from 0.63 to 0.99 (evenly distributed), whereas in inorganic rice fields ranged from 0 to 0.99 (uneven until evenly distributed). The dominant ants in organic rice fields were Camponotus sp, Solenopsis geminata, Anoplolepis gracilipes, and Paratrechina longicornis, whereas in the inorganic rice fields were Tapinoma sp, Solenopsis geminata, and Camponotus sp. The index of similarity between organic and inorganic rice fields showed a high and very high degree of similarity based on the type of feed. Physical factors of high soil and air humidity environment, high soil and air temperature increased the diversity and abundance of ants in the rice fields ecosystem. Factors soil pH in accordance with the growth of soil Arthropods were neutral or slightly acidic. Chemical factors of organic matter content, Nitrogen (N), the content of C organic, and a high P total content increased the diversity and abundance of ants. Keywords: diversity and abundance, ants, organic and inorganic rice fields
Metode Monitoring Resistensi Populasi Aedes aegypti Dengan Penetapan Konsentrasi Diagnostik Sari, Vicka Kusuma; Tarwotjo, Udi; Hadi, Mochamad
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.446 KB) | DOI: 10.14710/bioma.20.2.105-112

Abstract

Aedes aegypti is the main vector of dengue fever disease. The most effective way to prevent dengue fever is cutting the spreading chain of dengue fever by controlling the vector with using conventional chemical insecticides. The used of insecticide intensively has became the main factor of resistance in Tembalang, then monitoring is needed to determine the change of A. aegypti susceptibility status against the insecticide. The aim of this research is to determine the sensitivity of A. aegypti population from five locations in Tembalang against pyrethroid synthetic insecticide, and to determine the validity of the diagnostic concentration as a method of monitoring resistance A. aegypti population in Tembalang. This research was conducted in Laboratory of Ecology and Biosystematics Department of Biology Faculty of Science and Mathematics, Diponegoro University Semarang. The procedure of this research were: larvae collection (A. aegypti), insect breeding test, sensitivity test including bioassay, determination of diagnostic concentration and validation. The result showed that the susceptibility level of five population was not significantly different since all populations are still susceptible, with the LC50 range between 0,0031-0,0043% and FR range between 1-1,39 (<4). The result of validation test of diagnostic concentration was 0,0038%, valid as a monitoring method of the resistance of A. aegypti population against pyrethroid synthetic insecticide in Tembalang because c2 value of the five population was lower than the c2 table (df = 1; α = 0,05) = 3,84. Keywords : A. aegypti, monitoring resistance, LC50, diagnostic concentration..
THE TOOL OF RESISTANCE MONITORING OF PLUTELLA XYLOSTELLA, (L.) AND ITS FIELD VALIDATION USING EMAMECTIN BENZOATE AND DIAGNOSTIC CONCENTRATION DETERMINATION Tarwotjo, Udi; Situmorang, Jesmandt; Martono, Edhy; Rahadian, Rully
NICHE Journal of Tropical Biology Vol. 3, No. 1, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.3.1.50-54

Abstract

In Indonesia, cabbage pest control is still heavily dependent on the use of insecticides. Although insecticides have many advantages, i.e., reducing the populations of pest quickly, easy to use, and economically advantageous, but it has the negative impact especially the emergence of resistant pest species.The objective of this study are: (1) to monitore the resistance of Plutella xylostella population toward insecticide residues of emamectin benzoate by diagnostic concentration determination, (2) to validate the diagnostic concentration in the field. P. xylostella population were collected from some cabbage farms in Central Java i.e., Bandungan, Sumowono, Cepogo, Kejajar, Kertek, Tawangmangu, Plalar, Puasan, Kaponan, and Keteb.Determination of LC90 was tested using probit analysis. The results showed that five of the eleven tested populations have sensitivity c2 value < c2 table, namely population Selo, Gedongsongo, Gondosuli, Kejajar, and Plalar. The determinated diagnostic concentration is LC90 = 2443.99 ppb with the lowest concentration limits 1213.67 ppb and the highest is 4921.53 ppb, because of the testing results of the sensitivity of all tested populations are still susceptible to emamectin benzoate with mortality > 90% at a concentration of 2000 ppb. The results of validation diagnostic concentration (2443.99 ppb), indicating the mortality percentage caused by eleventh population ranged from 82.76% to 95%, the value of c2 value is smaller than c2 table (df = 1; ? = 0.05) = 3.84. Therefore the diagnostic concentration (ppb 2443.99) is valid for effective monitoring tool for the development of resistance of P. xylostella population. The conventional method to monitor resistance is less sensitive to the resistance emerging occurrence. Therefore, it is necessary to develop monitoring methods which are more sensitive to small changes in the frequency of resistance, by using diagnostic concentration.
Resistance Monitoring of Nilaparvata lugens Stall against Pymetrozine Insecticide with Determination of Diagnostic Concentrations Murtiati, Sri; Tarwotjo, Udi; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.28237

Abstract

Nilaparvata lugens Stall is one of the main insect pests on rice crops. Intensive control of this pests using insecticides has resulted in the development of insect resistance. This study aimed to find out the level of resistance of the N. lugens population to pymetrozine insecticides by determining the diagnostic concertations. N. lugens was collected from five endemic areas in Central Java Province from October 2019 to June 2020. The data from the bioassay test were analyzed with probit analysis to obtain the LC50 value. The results of the sensitivity test showed that the Kajen population has the highest RF value (2.47), while the Karanganyar population which has the lowest RF value (1) was the most sensitive population. The determined diagnostic concentration was LC95 = 25.52 ppm with the lowest concentration limit of 7.67 ppm and the highest of 30.05 ppm. To conclude, the determined diagnostic concentration (LC95 = 25.52 ppm) is effective for detecting the susceptibililty of N. lugens population. This finding would be beneficial for monitoring resistance of N. lugens population against pymetrozine insecticides in the field.
KERAGAMAN SERANGGA OPT DAN MUSUH ALAMI DI LAHAN KACANG HIJAU SEBELUM DAN PASCA PANEN Dwiyani Anjar Martitik; Mochamad Hadi; Udi Tarwotjo
Jurnal Akademika Biologi Vol. 7 No.2 April 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Serangga dapat berperan sebagai OPT ataupun musuh alami di suatu ekosistem pertanian. Penelitian ini bertujuan untuk mengetahui jenis serangga OPT dan musuh alami dominan, keragaman serta pengaruh faktor fisik lingkungan terhadap keragaman serangga OPT dan musuh alami. Pengambilan sampel dilakukan dua kali yaitu pada masa sebelum dan pasca panen. Setiap pengambilan sampel menggunakan dua metode, yaitu jaring ayun dan metode light trap. Hasil penelitian menunjukkan jumlah individu dan jumlah jenis yang didapatkan sebelum panen lebih tinggi dibandingkan pasca panen. OPT yang dominan, yaitu famili Chrysomelidae, Curculionidae, Sciaridae, Pentatomidae, Cicadellidae, dan Pyralidae. Sedangkan musuh alami yang dominan yaitu famili Staphylinidae, Coccinelidae, Carabidae, Miridae, Aelothripidae, Tachinidae, Formicidae, Salticidae dan Thomisidae. Keragaman jenis OPT dan musuh alami termasuk dalam kategori sedang. Indeks kemerataan OPT sebelum dan pasca panen serta musuh alami sebelum panen termasuk dalam kategori merata sedangkan musuh alami pasca panen termasuk dalam kategori tidak merata. Indeks kesamaan antara kedua musim pengambilan sampel menunjukkan tingkat kesamaan yang bervariasi. Faktor fisik lingkungan kelembaban udara, suhu udara, kecepatan angin dan intensitas cahaya matahari berpengaruh terhadap aktivitas OPT maupun musuh alami di lahan penelitian.
Kelimpahan dan Keanekaragaman Serangga pada Sawah Organik dan Konvensional di Sekitar Rawa Pening Deni Elisabeth; Jafron Wasiq Hidayat; Udi Tarwotjo
Jurnal Akademika Biologi Vol. 10 No. 1 Januari 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (699.543 KB)

Abstract

Serangga merupakan hewan yang memiliki jumlah terbesar di bumi, sehingga dengan dominasi tersebut menjadikan serangga sebagai penyambung kebutuhan dalam siklus energi dengan berbagai peran yang dilakukan. Penelitian ini bertujuan untuk mengetahui mengetahui kelimpahan dan keakaragaman serangga pada sawah organik dan sawah konvensional di sekitar Rawa Pening, mengetahui jenis serangga pada sawah padi organik dan sawah konvensional di Rawa Pening dan untuk mengetahui kondisi faktor lingkungan yang mempengaruhi kelimpahan dan keanekaragaman serangga di sawah organik dan sawah konvensional. Pengambilan sampel dilakukan sebanyak tiga kali.Pengambilan sampel menggunakan metode sweep net dan di analisi dengan indeks keanekaragaman jenis Shannon Wiener dan indeks kelimpahan. Hasil penelitian yang didapatkan yaitu jumlah individu pada sawah organic individu lebih tinggi (52 individu) dibandingkan dengan sawah konvensional (46) individu) dan pada jumlah jenis sawah organik lebih rendah dibandingkan dengan sawah konvensional. Serangga yang dominan pada sawah organik yaitu Archimantis, Kosciuscola, Conocephalus, Axion, Pantala, Acisoma dan Leptocorisa. Keanekaragaman pada sawah organik dan konvensional termasuk kategori sedang yaitu pada sawah pada sawah organik 2,04 dan sawah konvensional 2,40, mengindikasikan kestabilan lingkungan yang sedang. Indeks kemerataan pada sawah organik yaitu 0,79 dan pada sawah konvensional 0,88 yang termasuk dalam kategori merata. Faktor fisik lingkungan seperti kelembaban udara 80-95 %, kecepatan angin 10-15m/s, pada temperature udara 20-22˚C dan pada intensitas cahaya matahari 5; masih sesuai bagi aktivitas serangga di lahan persawahan.
Struktur komunitas makroarthropoda tanah di lahan pertanian durian di kawasan Gunungpati Febri Edo; Udi Tarwotjo; Rully Rahadian
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.951 KB) | DOI: 10.14710/jbt.2.2.48-53

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The objective of this study is to compare community structures, which include diversity, abundance, evenness, and macroarthropod dominance of monoculture durian farmland, polyculture durian farmland and non-durian farmland (arabica coffee) in Gunungpati region. This research is an exploratory observation carried out from February to March 2019. Samplingwas done using pitfal trap. The identification of samples was carried out in the Diponegoro University Ecology and Biosystematics laboratory. Data analysis was carried out by calculating diversity index (H'), evenness (e), dominance (D), richness, Sorenson test and Hutcheson t-test. The results showed that arthropods in monoculture farmland were found 195 individuals, in polyculture farmland were found 181 individuals and in arabica coffee farmland were found 229 arthropods. Diversity index (H') and evenness (e) in monoculture farmland were lower than in polyculture farmland and arabica coffee farmland. The three areas are dominated by the family Formicidae. The three fields have a high degree of similarity. Overall 8 families were found as predators, 4 families as herbivores, 3 families as detritivores and 1 family as decomposers.
Struktur komunitas mikroartropoda tanah di lahan pertanian kentang di Desa Sembungan Dataran Tinggi Dieng, Jawa Tengah Insan Shiddiq; Rully Rahadian; Udi Tarwotjo
Jurnal Biologi Tropika Vol. 3, No. 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.937 KB) | DOI: 10.14710/jbt.1.1.17-23

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The use of manure in the area of  Dieng Plateau is likely to increase from year to year. Excessive use of fertilizer has the potential to disturb soil fauna. The objective of  this research are to know the effect of intensively use of pure chicken manure and chicken manure in combination with husk in the Dieng Plateau potato farm, Sembungan Village to structure of soil microarthropods. Sampling was located in two station of potatoes farmland that uses pure chicken manure (chicken manure: CM) and chicken manure in combination with husk (chicken manure + husk :CMS). Soil sampling was applied using transect line method with five sampling points. The structure of soil microarthropods communities was analysed into relative abundance, the Shannon-Wiener’s diversity index, the evenness index, the Sorensen’s similarity index, and Hutcheson’s t-test. The results showed that the density of soil microarthropods on potato farmland using chicken manure in combination with husk (5620 individuals/m2) was higher than farmland using pure chicken manure (1830 individual/m2). The dominant taxa in both farmland are Acari and Collembola. Based on the index of Sorensen similarities, the composition of soil microarthropods in both farmlands is relatively similar (76%). Statistically, the diversity of soil microarthropods between the two different potatoes farmland is significantly different.
Community structure of soil microarthropods in active and passive zones of Ngronggo landfill Salatiga Psn Masruri Sulistiyanto Ari; Rully Rahadian; Udi Tarwotjo
NICHE Journal of Tropical Biology Vol. 2, No. 2, Year 2019
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1934.201 KB) | DOI: 10.14710/niche.2.2.1-6

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Soil microarthropods play a key role in decomposing organic materials. The objectives of this study were to compare the structure of soil microarthropods communities in the active and passive zones of Ngronggo landfill Salatiga. The sampling of ten soil samples in each active and passive zones was performed for soil microarthropods extraction and soil physical-chemical analysis. Soil microarthropods were extracted using modified Barlese-Funnel. Soil microarthropods data were analyzed to determine abundance, diversity, and similarity. Physical-chemical factors were measured, including soil texture, heavy metal content (Pb, Cd, Cu, Zn, and Hg) and organic matter. The results show that all attributes of structure of soil microarthropods communities in active zone are higher than in passive zone, i.e., the density, the species richness, the species diversity, and the evenness. In both zones, the composition of soil microarthropods tend to be different.. Organic matter is likely the most important soil physicochemical factors to form the microarthropod structure in active and passive zones. This study contributes a new information about the role of soil microarthropods in decomposing the disposal waste of material and it would be benefit for developing a bioindicator of waste decomposition in landfill area.
Kondisi tanah sebelum dan sesudah ditambang di area galian pasir dan batu Rowosari Semarang berdasarkan struktur komunitas mikroartropoda dan indeks QBS-ar Rully Rahadian; Mochamad Hadi; Udi Tarwotjo; Wiatri Larasati; Noora Lailatul Husna
NICHE Journal of Tropical Biology Vol. 4, No. 2, Year 2021
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.4.2.40-45

Abstract