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All Journal Akuntansi Krida Wacana Indonesian Journal of Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Ngadiman ., Ngadiman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology

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 1 
Purifi cation and Characterization of Protease From Bacillus sp. TBRSN- 1 Margino, Sebastian; ., Ngadiman
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.697 KB)

Abstract

Potato Cyst Nematode (PCN), Globodera rostochiensis, is one of the important potato’s pests and causedeconomic looses up to 70% in the several centrals of potato plantations in Indonesia. PCN’s shell componentof egg shell containing chitin (inner layer) and viteline/ protein (outer layer). The purpose of this researchwas to purify of protease Bacillus sp. TBRSN-1, isolate from tomato’s rhizosfer in Yogyakarta province. Thepurifi ed protease could be used for cutting the life cycle of PCN. Results showed that Bacillus sp. TBRSN-1could produce extracellular protease and purifi cation using DEAE-cellulose ion-exchange chromatographyand Sephacryl S-300 gel fi ltration chromatography resulted in specifi c activity 4.31 fold and 1.68% recovery.Analysing using SDS-PAGE 12.5% and molecular weight 48.1 kDa. Km and Vmax values of the protease forcasein substrate were 7.83 mg/ml and 4.03 μg/h, respectively. The optimum activity at the temperature30oC and pH 7.0.Keywords : protease, purifi cation, indigenous Bacillus sp. TBRSN-1
FITOREMEDIASI KANDUNGAN KROMIUM PADA LIMBAH CAIR MENGGUNAKAN TANAMAN AIR Safarrida, Anna; ., Ngadiman; Widada, Jaka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 2, No 2 (2015): December 2015
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.874 KB) | DOI: 10.29122/jbbi.v2i2.509

Abstract

Existence of heavy metals in industrial waste is gaining global attention since their negative impact to environment. One of the efforts to solve the problem was to use plant to absorb metal in liquid medium, known as rhizofiltration. This research was aimed to select aquatic plants which showed relative resistantce and susceptibility to chromium. Four species of aquatic plants (Pistia stratiotes, Eichhornia crassipes, Lemna minor and Salvinia sp.) were grown in artificial medium (Hoagland) supplemented with 0, 1, 2, 4 and 8 ppm chromium. The plants resistance and absorption toward chromium was observed based on the morphology and chromium content in their biomass. Based on their resistance to and absorption of chromium, the selected plants were tested further in liquid waste of tanning industry. In Hoagland medium, Salvinia sp. demonstrated 67.2% higher resistance and absorption toward chromium while that of P. stratiotes 20.3% lower compared to other plants which were tested. This result could be applicable in reducing such environmental pollutant as the heavy metal chromium from industrial waste. Keywords: Phytoremediation, chromium, Hoagland medium, aquatic plants, liquid waste ABSTRAKLogam berat dalam limbah industri merupakan bahan pencemar lingkungan yang mendapatkan perhatian global. Salah satu upaya untuk mengatasi masalah tersebut adalah memanfaatkan tanaman untuk menyerap logam dalam medium cair atau dikenal sebagai fitoremediasi. Penelitian ini bertujuan untuk mengetahui tanaman air lokal yang tahan dan peka secara relatif terhadap kromium. Empat spesies tanaman air (Pistia stratiotes, Eichhornia crassipes, Lemna minor, dan Salvinia sp.) ditumbuhkan pada medium buatan (Hoagland) yang dipasok kromium 0, 1, 2, 4, dan 8 ppm. Pengujian toleransi tanaman dan serapan terhadap kromium dilakukan berdasarkan pengamatan morfologis serta analisis kadar kromium dalam biomasa. Berdasarkan daya tahan dan serapan kromium, tanaman terseleksi diujikan lebih lanjut dalam limbah cair industri penyamakan kulit. Dalam medium Hoagland, Salvinia sp. mempunyai ketahanan dan serapan kromium lebih tinggi sebesar 67,2% sedangkan P. stratiotes mempunyai ketahanan dan serapan kromium lebih rendah sebesar 20,3% dibandingkan tanaman lain yang diujikan. Hasil penelitian ini dapat diterapkan untuk mengurangi bahan pencemar lingkungan berupa logam berat kromium dari limbah industri.Kata Kunci: Fitoremediasi, kromium, medium Hoagland, tanaman air, limbah cair
Pengaruh Ukuran Perusahaan, Persistensi Laba Akuntansi, Struktur Modal, dan Variabel Indikator Terhadap Koefisien Respon Laba Akuntansi Perusahaan yang Terdaftar di BEI untuk Tahun 2009 ., Ngadiman; Hartini, Yurike
Akuntansi Krida Wacana vol. 11 no. 2 November 2011
Publisher : Akuntansi Krida Wacana

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Abstract

FITOREMEDIASI KANDUNGAN KROMIUM PADA LIMBAH CAIR MENGGUNAKAN TANAMAN AIR Safarrida, Anna; ., Ngadiman; Widada, Jaka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.874 KB) | DOI: 10.29122/jbbi.v2i2.509

Abstract

Existence of heavy metals in industrial waste is gaining global attention since their negative impact to environment. One of the efforts to solve the problem was to use plant to absorb metal in liquid medium, known as rhizofiltration. This research was aimed to select aquatic plants which showed relative resistantce and susceptibility to chromium. Four species of aquatic plants (Pistia stratiotes, Eichhornia crassipes, Lemna minor and Salvinia sp.) were grown in artificial medium (Hoagland) supplemented with 0, 1, 2, 4 and 8 ppm chromium. The plants resistance and absorption toward chromium was observed based on the morphology and chromium content in their biomass. Based on their resistance to and absorption of chromium, the selected plants were tested further in liquid waste of tanning industry. In Hoagland medium, Salvinia sp. demonstrated 67.2% higher resistance and absorption toward chromium while that of P. stratiotes 20.3% lower compared to other plants which were tested. This result could be applicable in reducing such environmental pollutant as the heavy metal chromium from industrial waste. Keywords: Phytoremediation, chromium, Hoagland medium, aquatic plants, liquid waste ABSTRAKLogam berat dalam limbah industri merupakan bahan pencemar lingkungan yang mendapatkan perhatian global. Salah satu upaya untuk mengatasi masalah tersebut adalah memanfaatkan tanaman untuk menyerap logam dalam medium cair atau dikenal sebagai fitoremediasi. Penelitian ini bertujuan untuk mengetahui tanaman air lokal yang tahan dan peka secara relatif terhadap kromium. Empat spesies tanaman air (Pistia stratiotes, Eichhornia crassipes, Lemna minor, dan Salvinia sp.) ditumbuhkan pada medium buatan (Hoagland) yang dipasok kromium 0, 1, 2, 4, dan 8 ppm. Pengujian toleransi tanaman dan serapan terhadap kromium dilakukan berdasarkan pengamatan morfologis serta analisis kadar kromium dalam biomasa. Berdasarkan daya tahan dan serapan kromium, tanaman terseleksi diujikan lebih lanjut dalam limbah cair industri penyamakan kulit. Dalam medium Hoagland, Salvinia sp. mempunyai ketahanan dan serapan kromium lebih tinggi sebesar 67,2% sedangkan P. stratiotes mempunyai ketahanan dan serapan kromium lebih rendah sebesar 20,3% dibandingkan tanaman lain yang diujikan. Hasil penelitian ini dapat diterapkan untuk mengurangi bahan pencemar lingkungan berupa logam berat kromium dari limbah industri.Kata Kunci: Fitoremediasi, kromium, medium Hoagland, tanaman air, limbah cair
Co-Authors ., Sohidin Andini, Thoifah Asri Anna Safarrida, Anna Elvia Ivada JAKA WIDADA Kusuma, Alfiana Rosita Laras Puspandari, Yeni Putri Sebastian Margino Sisyawati, Tatas Sri Sumaryati Yurike Hartini