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Purifi cation and Characterization of Protease From Bacillus sp. TBRSN- 1 Margino, Sebastian; ., Ngadiman
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Potato Cyst Nematode (PCN), Globodera rostochiensis, is one of the important potato’s pests and causedeconomic looses up to 70% in the several centrals of potato plantations in Indonesia. PCN’s shell componentof egg shell containing chitin (inner layer) and viteline/ protein (outer layer). The purpose of this researchwas to purify of protease Bacillus sp. TBRSN-1, isolate from tomato’s rhizosfer in Yogyakarta province. Thepurifi ed protease could be used for cutting the life cycle of PCN. Results showed that Bacillus sp. TBRSN-1could produce extracellular protease and purifi cation using DEAE-cellulose ion-exchange chromatographyand Sephacryl S-300 gel fi ltration chromatography resulted in specifi c activity 4.31 fold and 1.68% recovery.Analysing using SDS-PAGE 12.5% and molecular weight 48.1 kDa. Km and Vmax values of the protease forcasein substrate were 7.83 mg/ml and 4.03 μg/h, respectively. The optimum activity at the temperature30oC and pH 7.0.Keywords : protease, purifi cation, indigenous Bacillus sp. TBRSN-1
FITOREMEDIASI KANDUNGAN KROMIUM PADA LIMBAH CAIR MENGGUNAKAN TANAMAN AIR Safarrida, Anna; ., Ngadiman; Widada, Jaka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 2, No 2 (2015): December 2015
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Existence of heavy metals in industrial waste is gaining global attention since their negative impact to environment. One of the efforts to solve the problem was to use plant to absorb metal in liquid medium, known as rhizofiltration. This research was aimed to select aquatic plants which showed relative resistantce and susceptibility to chromium. Four species of aquatic plants (Pistia stratiotes, Eichhornia crassipes, Lemna minor and Salvinia sp.) were grown in artificial medium (Hoagland) supplemented with 0, 1, 2, 4 and 8 ppm chromium. The plants resistance and absorption toward chromium was observed based on the morphology and chromium content in their biomass. Based on their resistance to and absorption of chromium, the selected plants were tested further in liquid waste of tanning industry. In Hoagland medium, Salvinia sp. demonstrated 67.2% higher resistance and absorption toward chromium while that of P. stratiotes 20.3% lower compared to other plants which were tested. This result could be applicable in reducing such environmental pollutant as the heavy metal chromium from industrial waste. Keywords: Phytoremediation, chromium, Hoagland medium, aquatic plants, liquid waste ABSTRAKLogam berat dalam limbah industri merupakan bahan pencemar lingkungan yang mendapatkan perhatian global. Salah satu upaya untuk mengatasi masalah tersebut adalah memanfaatkan tanaman untuk menyerap logam dalam medium cair atau dikenal sebagai fitoremediasi. Penelitian ini bertujuan untuk mengetahui tanaman air lokal yang tahan dan peka secara relatif terhadap kromium. Empat spesies tanaman air (Pistia stratiotes, Eichhornia crassipes, Lemna minor, dan Salvinia sp.) ditumbuhkan pada medium buatan (Hoagland) yang dipasok kromium 0, 1, 2, 4, dan 8 ppm. Pengujian toleransi tanaman dan serapan terhadap kromium dilakukan berdasarkan pengamatan morfologis serta analisis kadar kromium dalam biomasa. Berdasarkan daya tahan dan serapan kromium, tanaman terseleksi diujikan lebih lanjut dalam limbah cair industri penyamakan kulit. Dalam medium Hoagland, Salvinia sp. mempunyai ketahanan dan serapan kromium lebih tinggi sebesar 67,2% sedangkan P. stratiotes mempunyai ketahanan dan serapan kromium lebih rendah sebesar 20,3% dibandingkan tanaman lain yang diujikan. Hasil penelitian ini dapat diterapkan untuk mengurangi bahan pencemar lingkungan berupa logam berat kromium dari limbah industri.Kata Kunci: Fitoremediasi, kromium, medium Hoagland, tanaman air, limbah cair
Pengaruh Ukuran Perusahaan, Persistensi Laba Akuntansi, Struktur Modal, dan Variabel Indikator Terhadap Koefisien Respon Laba Akuntansi Perusahaan yang Terdaftar di BEI untuk Tahun 2009 ., Ngadiman; Hartini, Yurike
Akuntansi Krida Wacana vol. 11 no. 2 November 2011
Publisher : Akuntansi Krida Wacana

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Abstract

IDENTIFIKASI GAYA BELAJAR SISWA AKUNTANSI SMK X TAHUN PELAJARAN 2017/2018 Puspandari, Yeni Putri; ., Ngadiman; ., Sohidin
Tata Arta : Jurnal Pendidikan Akuntansi Vol 3, No 2 (2017): Jurnal Pendidikan Akuntansi
Publisher : FKIP Universitas Sebelas Maret

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Abstract

ABSTRACTThis study aims to determine the tendency of learning styles visual, auditory, and kinesthetic students. Population in this research is all students of Accounting SMK X Academic Year 2017/2018. The selected sam-ples were class X, XI, and XI of 137 students, with stratified random sampling technique. The method used in this research is quantitative descriptive. The data were collected by questionnaire of visual, auditory, and kin-esthetic learning style. Data analysis used is descriptive statistic to describe the trend learning style, then look for tendency of learning style every student to can be calculated percentage of student overall The results of this study are as follows. First, the trend of visual learning style of 45% students at high level, 40% moderate, and 15% low. The trend of 21% auditory student learning style at high, 70%, and 9% is low. The trend of learning style of kinestetik students 4% at high level, 66% medium, and 30% low. Second, Accounting students have a tendency of visual learning style as much as 57%, 30% of auditory student, and 13% of kinesthetic stu-dent.Keywords: visual learning style, auditory learning style, kinesthetic learning styleABSTRAKPenelitian ini bertujuan untuk mengetahui kecenderungan gaya belajar visual, auditori, dan kinestetik siswa. Populasi dalam penelitian ini adalah seluruh siswa Akuntansi SMK X tahun pelajaran 2017/2018. Sampel yang terpilih adalah kelas X, XI, dan XI sejumlah 137 siswa, dengan teknik pengambilan sampel strat-ified random sampling. Penelitian ini menggunakan metode deskriptif kuantitatif. Pengumpulan data dil-aksanakan dengan angket gaya belajar visual, auditori, dan kinestetik. Statistik deskriptif digunakan untuk an-alisis data kemudian mencari kecenderungan gaya belajar setiap siswa dan menghitung persentase siswa kese-luruhan. Hasil penelitian adalah sebagai berikut ini. Pertama, kecenderungan gaya belajar siswa visual 45% pada tingkatan tinggi, 40% sedang, dan 15% rendah. Kecenderungan gaya belajar siswa auditori 21% pada tingkatan tinggi, 70% sedang, dan 9% rendah. Kecenderungan gaya belajar siswa kinestetik 4% pada tingkatan tinggi, 66% sedang, dan 30% rendah. Kedua, siswa Akuntansi memiliki kecenderungan gaya belajar visual sebanyak 57%, 30% siswa auditori, dan 13% siswa kinestetik.Kata kunci: gaya belajar visual, gaya belajar auditori, gaya belajar kinestetik
PERBEDAAN FINANCIAL LITERACY MAHASISWA PENDIDIKAN AKUNTANSI UNS BERDASARKAN KECERDASAN INTELEKTUAL DAN TEMPAT TINGGA Sisyawati, Tatas; ., Ngadiman; ., Sohidin
Tata Arta : Jurnal Pendidikan Akuntansi Vol 3, No 3 (2017): Jurnal Pendidikan Akuntansi
Publisher : FKIP Universitas Sebelas Maret

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Abstract

AbstractThis study is aimed to determine the differences in financial literacy of the student in the Accounting Education Department of Sebelas Maret University Surakarta based on their intelligence and residence. This research method is a comparative causal research with sampling technique in the form of simple ran-dom sampling. The data collection method in the study was using tests and questionnaires. The analysis methods were using descriptive statitistic and hypothesis test in the form of one way anova test and t test. The result of the research shows that there are differences of financial literacy of the students of Account-ing Study Program of UNS based on intelligence and there is no difference of financial literacy of the stu-dents of Accounting Study Program of UNS based on their place of residence. These results indicate that students should develop positive financial habits and behavior. In addition universities and their depart-ments can provide the education in the field of financial literacy that can encourage students to perform well in financial management and to become a smart alumni.Keywords: Financial literacy, intelligence, place of residenceAbstrakPenelitian ini bertujuan untuk mengetahui perbedaan financial literacy mahasiswa Program Studi Pendidikan Akuntansi Universitas Sebelas Maret Surakarta berdasarkan kecerdasan intelektual dan tempat tinggal. Metode penelitian ini adalah penelitian kausal komparatif dengan teknik pengambilan sampel beru-pa simple random sampling. Pengumpulan data dalam penelitian menggunakan tes dan angket. Metode an-alisis yang digunakan adalah statitistik deskriptif dan uji hipotesis berupa uji one way anova dan uji t. Hasil penelitian menunjukkan bahwa terdapat perbedaan financial literacy mahasiswa prodi Pendidikan Akuntan-si UNS berdasarkan kecerdasan intelektual dan tidak terdapat perbedaan financial literacy mahasiswa prodi Pendidikan Akuntansi UNS berdasarkan tempat tinggal. Hasil tersebut menunjukkan bahwa mahasiswa harus mengembangkan kebiasaan dan perilaku keuangan yang positif. Selain itu universitas dan progam studi perlu memberikan pendidikan di bidang financial literacy sehingga dapat mendorong mahasiswa un-tuk melakukan pengelolaan keuangan secara lebih baik dan mampu menjadi alumni yang cerdas.Kata kunci: Financial literacy, kecerdasan intelektual, tempat tinggal
PENGARUH PENERAPAN MODEL PEMBELAJARAN STUDENT TEAMS ACHIEVEMENT DIVISION (STAD) DAN NUMBER HEADS TOGETHER (NHT) TERHADAP PRESTASI BELAJAR AKUNTANSI DITINJAU DARI AKTIVITAS BELAJAR SISWA Kusuma, Alfiana Rosita Laras; ., Ngadiman; Sumaryati, Sri
Tata Arta : Jurnal Pendidikan Akuntansi Vol 3, No 2 (2017): Jurnal Pendidikan Akuntansi
Publisher : FKIP Universitas Sebelas Maret

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Abstract

ABSTRACTThis research is conducted to find out: (1) The difference effect of the implementation Student Team Achievement Division (STAD) and Number Heads Together (NHT) toward the accounting students’ achievement learning. (2) The effect among students activity toward students’ achievement learning. (3) The interaction of learning method effect and learning activity toward achievement learning. The method that the writer used in this research was Quasi-experimental method. The population of this research was the students in eleventh grade of accounting class at SMK A. The sample of this research were two classes with 72 students that chosen with random sampling method. The data collected used tests, questionnaire and documentation. The trustworthiness used validity (Korelasi Product Moment), realibility (Cronbach Alpha), level of difficulty, and appropriateness. The technique of the data analysis was using ANAVA. The result of this research was: (1) There was a difference effect in the implementation of Student Team Achievement Division (STAD) and Number Heads Together (NHT) toward the students achievement learn-ing. It can be seen from Ph<Pk (0,014<0,05). (2) There was effect among learning activity toward stu-dents’ achievement learning. It can be seen from Ph<Pk (0,000<0,05). (3) There was interaction among students learning activity and learning methods toward students’ achievement learning. It can be seen from Ph<Pk (0,007<0,05).Keywords : Achievement Learning, Learning Activity, Learning MethodsABSTRAKPenelitian ini bertujuan untuk mengetahui: (1) Perbedaan pengaruh penerapan model Student Team Achievement Division (STAD) dan Number Heads Together (NHT) terhadap prestasi belajar mata pelaja-ran akuntansi. (2) Pengaruh aktivitas belajar terhadap prestasi belajar. (3) Interaksi pengaruh model pem-belajaran dan aktivitas belajar terhadap prestasi belajar. Metode yang digunakan dalam penelitian ini ada-lah eksperimental semu (Quasi-Eksperimental). Populasi penelitian ini adalah seluruh siswa kelas XI Akuntansi SMK A sejumlah 109 siswa. Sampel yang digunakan sejumlah 2 kelas atau 72 siswa diambil dengan teknik random sampling. Teknik pengumpulan data yang digunakan adalah tes, angket, dan doku-mentasi. Uji intrumen yang digunakan adalah validitas (Korelasi Poduct Moment), reliabilitas (Cronbach Alpha), tingkat kesukaran, dan daya beda. Teknik analisis data yang digunakan adalah ANAVA. Hasil penelitian menunjukkan : (1) Terdapat perbedaan pengaruh penerapan model Student Team Achievement Division (STAD) dan Number Heads Together (NHT) terhadap prestasi belajar akuntansi. Hal ini dapat dilihat dari hasil analisis variansi diperoleh Ph<Pk (0,014<0,05). (2) Terdapat pengaruh aktivitas belajar terhadap prestasi belajar. Hal ini dapat dilihat dari Ph<Pk (0,000<0,05). (3) Terdapat interaksi pengaruh model pembelajaran dan aktivitas belajar terhadap prestasi belajar. Hal ini dapat dilihat dari Ph<Pk (0,007<0,05).Kata kunci : Prestasi Belajar, Aktivitas Belajar, Model Pembelajaran.
PENGARUH KEAKTIFAN BERORGANISASI DAN PEMANFAATAN JAM BELAJAR DI RUMAH TERHADAP PRESTASI BELAJAR Andini, Thoifah Asri; ., Ngadiman; Ivada, Elvia
Tata Arta : Jurnal Pendidikan Akuntansi Vol 3, No 1 (2017): Jurnal Pendidikan Akuntansi
Publisher : FKIP Universitas Sebelas Maret

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Abstract

ABSTRACTThe purposes of this research were to investigate (1) the influence of the activeness in organization on students learning achievements at Accounting Department of State Vocational High School of X in 2016/2017; (2) the influence of utilization hours home study on students learning achievements at Accounting Department of State Vocational High School of X in 2016/2017. This research used the quantitative research method with the causal relationship design. Its population was all of the students in Grade X and XI Accounting Department of State Vocational High School of X in 2016/2017. The samples of research were all of the students as many as 98 in Grade X and XI Accounting of the school and were determined by using the non-probability sampling with sampling saturated. Its data were collected through questionnaire used to obtain data about the activeness in organization and utilization of study hour at home, while the documentation used to collect data on average value of report cards of students The data were validated by using the Pearson’s Product Moment at the significance level of 5%, and their reliability was tested by using the Cronbach’s Alpha formula. They were then analyzed by using the statistical, descriptive, and inferential model of analysis, namely: the multiple linear regression tests. The results concluded (1) there was a positive and significant influence between the activeness in organization on students learning achievements at Accounting Department of State Vocational High School of X in 2016/2017. It was shown from the calculations tcount > ttable (8,267 > 1,98525) at significance level 5% (0.000 < 0.05); (2) there was a positive and significant influence between the utilization hours home study on students learning achievements at Accounting Department of State Vocational High School of X in 2016/2017. It was shown from the calculations tcount > ttable (5,527 > 1,98525) at significance level 5%Keywords: The Activeness in Organization, Utilization Hours Home Study, Learning AchievementsABSTRAKTujuan penelitian ini adalah untuk mengetahui (1) pengaruh keaktifan berorganisasi terhadap prestasi belajar siswa Jurusan Akuntansi di SMK X tahun ajaran 2016/2017; (2) pengaruh pemanfaatan jam belajar di rumah terhadap prestasi belajar siswa Jurusan Akuntansi di SMK X tahun ajaran 2016/2017. Penelitian ini merupakan penelitian kuantitatif dengan desain penelitian hubungan kausal. Populasi penelitian ini adalah siswa kelas X dan XI Jurusan Akuntansi di SMK X. Sampel penelitian adalah seluruh siswa kelas X dan XI Jurusan Akuntansi di SMK X yang berjumlah 98 siswa yang ditentukan menggunakan teknik non-probability sampling jenis sampling jenuh. Angket dan dokumentasi digunakan sebagai alat pengumpulan data. Teknik analisis data menggunakan statistik deskriptif dan inferensial meliputi uji regresi linear berganda. Hasil penelitian menyimpulkan (1) terdapat pengaruh positif dan signifikan keaktifan berorganisasi terhadap prestasi belajar siswa Jurusan Akuntansi di SMK X tahun ajaran 2016/2017 dengan hasil thitung > ttabel (8,267 > 1,98525) pada taraf signifikansi 5% (0,000 < 0,05); (2) terdapat pengaruh positif dan signifikan pemanfaatan jam belajar di rumah terhadap prestasi belajar siswa Jurusan Akuntansi di SMK X tahun ajaran 2016/2017 dengan hasil thitung > ttabel (5,527 > 1,98525) pada taraf signifikansi 5% (0,000 < 0,05).Kata Kunci: Keaktifan Berorganisasi, Pemanfaatan Jam Belajar di Rumah, Prestasi Belajar
FITOREMEDIASI KANDUNGAN KROMIUM PADA LIMBAH CAIR MENGGUNAKAN TANAMAN AIR Safarrida, Anna; ., Ngadiman; Widada, Jaka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.874 KB) | DOI: 10.29122/jbbi.v2i2.509

Abstract

Existence of heavy metals in industrial waste is gaining global attention since their negative impact to environment. One of the efforts to solve the problem was to use plant to absorb metal in liquid medium, known as rhizofiltration. This research was aimed to select aquatic plants which showed relative resistantce and susceptibility to chromium. Four species of aquatic plants (Pistia stratiotes, Eichhornia crassipes, Lemna minor and Salvinia sp.) were grown in artificial medium (Hoagland) supplemented with 0, 1, 2, 4 and 8 ppm chromium. The plants resistance and absorption toward chromium was observed based on the morphology and chromium content in their biomass. Based on their resistance to and absorption of chromium, the selected plants were tested further in liquid waste of tanning industry. In Hoagland medium, Salvinia sp. demonstrated 67.2% higher resistance and absorption toward chromium while that of P. stratiotes 20.3% lower compared to other plants which were tested. This result could be applicable in reducing such environmental pollutant as the heavy metal chromium from industrial waste. Keywords: Phytoremediation, chromium, Hoagland medium, aquatic plants, liquid waste ABSTRAKLogam berat dalam limbah industri merupakan bahan pencemar lingkungan yang mendapatkan perhatian global. Salah satu upaya untuk mengatasi masalah tersebut adalah memanfaatkan tanaman untuk menyerap logam dalam medium cair atau dikenal sebagai fitoremediasi. Penelitian ini bertujuan untuk mengetahui tanaman air lokal yang tahan dan peka secara relatif terhadap kromium. Empat spesies tanaman air (Pistia stratiotes, Eichhornia crassipes, Lemna minor, dan Salvinia sp.) ditumbuhkan pada medium buatan (Hoagland) yang dipasok kromium 0, 1, 2, 4, dan 8 ppm. Pengujian toleransi tanaman dan serapan terhadap kromium dilakukan berdasarkan pengamatan morfologis serta analisis kadar kromium dalam biomasa. Berdasarkan daya tahan dan serapan kromium, tanaman terseleksi diujikan lebih lanjut dalam limbah cair industri penyamakan kulit. Dalam medium Hoagland, Salvinia sp. mempunyai ketahanan dan serapan kromium lebih tinggi sebesar 67,2% sedangkan P. stratiotes mempunyai ketahanan dan serapan kromium lebih rendah sebesar 20,3% dibandingkan tanaman lain yang diujikan. Hasil penelitian ini dapat diterapkan untuk mengurangi bahan pencemar lingkungan berupa logam berat kromium dari limbah industri.Kata Kunci: Fitoremediasi, kromium, medium Hoagland, tanaman air, limbah cair