<![CDATA[Inflammation is a natural defense response of the body when an infection occurs. However, excessive inflammatory reactions can be triggered by lipopolysaccharides (LPS), which are key immunogenic components of bacterial capsules or cell walls. These reactions can lead to harmful effects on the body. Black rice (Oryza sativa L. indica) is a natural source rich in phenols, flavonoids, and antioxidant anthocyanins, which act as immunomodulators. This study aimed to investigate the effect of black rice ethanol extract as an anti-inflammatory agent on the proliferation of polymorphonuclear cells (PMN) in the spleen of rats induced by lipopolysaccharides. The study involved 27 mice, which were given a 400 mg/kg BW dose of black rice ethanol extract for 14 days. Afterward, the rats were induced with 0.3 mg/kg BW LPS intraperitoneally 24 hours before euthanasia using CO2. The spleens were then collected to measure the size of the germinal center diameter. 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