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All Journal Jurnal Ilmiah Kedokteran Wijaya Kusuma Hang Tuah Medical Journal
SULISTIANA PRABOWO
Fakultas Kedokteran Universitas Hang Tuah Surabaya
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Pengaruh Pemberian Ekstrak Kulit Buah Alpukat (Persea Americana) Terhadap Kadar Malondialdehida (MDA) Jaringan Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Parasetamol Dosis Tinggi YUNITA SURYA PRATIWI; SULISTIANA PRABOWO
HANG TUAH MEDICAL JOURNAL Vol 15 No 2 (2018): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

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Abstract Administration of high-dose paracetamol may increase metabolic pathways that produce N-acetyl-p-benzoquinonimine (NAPQI). NAPQI is reactive substance, resulting in hepatic tissue damage and increase liver MDA level. Avocado peel extract contains flavonoids that act as antioxidants so it is expected may decrease liver MDA level of high-dose paracetamol administration. This research used 24 male Wistar rats divided into 3 groups: group of rats fed with standard food, group of rats given paracetamol 1,750 mg/kg BW on day 8, and group of rats given avocado peel extract 1,400 mg/kg/day for 8 days and paracetamol 1750mg/kg BW on day 8. Liver MDA levels were checked on day 9 by thiobarbituric acid (TBA) method. The One-Way ANOVA test showed that the liver MDA level of the group of rats given high dose of paracetamol (x=312.38±47.830 nmol/g) was significantly higher (p = 0.001) compared to the liver MDA level of the group of rats fed with standard food (x=184.50±57.021 nmol/g). The liver MDA level of group of rats given high-dose paracetamol and avocado peel extract (x=268.50±91.834 nmol/g) did not significantly decrease (p =0.213) compared to the liver MDA level of group of rats given high dose of paracetamol (x=312.38±47.830 nmol/g). The conclusion of this research showed that high-dose paracetamol significantly increased liver MDA level and avocado peel extract tend to decrease liver MDA level because avocado peel consists flavonoid, hydroxynnamic acid, keratinoid, vitamin C, dan vitamin E that function as antioxidants.
PENGARUH PEMBERIAN EKSTRAK DAUN KELOR (Moringa oleifera) TERHADAP KADAR KOLESTEROL HDL TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR HIPERGLIKEMIA DENGAN INDUKSI ALOKSAN Lybelary Dewi Satrianawaty; Tri Martini Sumarno; Sulistiana Prabowo
HANG TUAH MEDICAL JOURNAL Vol 17 No 1 (2019): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

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ABSTRACT Background : Diabetes mellitus (DM) is a chronic metabolic syndrome characterized by an increase in blood glucose levels due to a lack of insulin, less effective insulin action or both. Hyperglycemia can cause a decrease in HDL cholesterol. Alloxan can reduce HDL cholesterol due to the mechanism of ROS formation. Moringa contains antioxidants that can prevent oxidative stress so that it is expected to increase HDL cholesterol levels. Method : This study used 24 male Wistar rats divided into 3 groups. The first group was given standard feed without alloxan induction, the second group was induced by 150 mg/kgBW intraperitoneal alloxan in the first day, and the third group was induced by alloxan in the first day and given 600 mg/kgBW Moringa leaf extract for 14 days begining at the fourth day. At the end of the study a serum HDL cholesterol levels were measured in each group.Result : One Way Anova test results showed significant decrease of serum HDL cholesterol (p = 0.024) in the alloxan-induced animal group (x = 26.88 mg/dL) compared to the group of rats fed with standard feed without alloxan induction (x = 33.13 mg/dL). There was no significant increase (p = 0.174) of HDL cholesterol in the group of rats that were induced by alloxan and given Moringa leaf extract (x = 29.25 mg/dL) compared to the group of rats that were induced by alloxan without being given Moringa leaf extract (x = 26, 88 mg/gL).Conclusion : this study showed that alloxan significantly reduced serum HDL cholesterol levels and the administration of Moringa leaf extract tended to increase serum HDL cholesterol levels in white rats induced by alloxan because flavonoids and phenols in Moringa leaves acted as antioxidants, but the increase was not statistically significant.
Pengaruh Ekstrak Daun Dan Buah Ketapang Terhadap Malondialdehida Pankreas Rattus Norvegicus Jantan yang Diinduksi Aloksan dan Diet Tinggi Lemak LYBELARY DEWI SATRIANAWATY; FRANSISKA CHRISTELA; ANTHONY ANUGERAH JOSHUA; SULISTIANA PRABOWO
HANG TUAH MEDICAL JOURNAL Vol 17 No 1 (2019): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

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ABSTRACT Background : Diabetes mellitus is a chronic disease caused by high levels of blood glucose. Hyperglycemia will cause the formation of free radicals which can damage cells. To reduce this effect, antioxidants are needed. Aloksan is a chemical that can damage beta cells, resulting in decreased insulin production. Ketapang (Terminalia catappa) is a plant that can be used because of its antioxidant content. The administration of ketapang extract is thought to reduce levels of malondialdehyde. Malondialdehyde is a biomarker for the occurrence of lipid peroxidation in the body caused by an increase in free radicals due to the occurrence of oxidative stress. Metode : 32 male rats divided into 4 groups. Three groups will be given high-fat diet for 7 days followed by induced alloxan at a dose of 90 mg / kgBW to increase blood glucose levels and pancreatic malondialdehyde levels. Then 2 groups will be given treatment for 14 days. The first treatment group was given Ketapang extract with a dose of 600 mg / kgBW and the second treatment group which was given glibenclamide using a dose of 0.09 mg / kgBW. Results: administration of ketapang leaf and fruit extract reduced the levels of molondialdehyde in Wistar rats given a high fat diet and induced by alloxan, but did not significant (p = 0.916). There was a significant reduction in malondialdehyde in Wistar rats given glibenclamide (p = 0.016). Conclusions: the administration of ketapang leaf and fruit extract were able to reduce the levels of pancreatic malondialdehyde given a high-fat diet and induced by alloxan.
Peran Ekstrak Anggur Laut terhadap Penurunan Kadar Kolesterol LDL Rattus norvegicus Jantan yang Mendapat Diet Tinggi Lemak RONALDO LAU; SULISTIANA PRABOWO; RIAMI
HANG TUAH MEDICAL JOURNAL Vol 17 No 2 (2020): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

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Abstract Background: High fat diet increase the absorption of lipid in the intestinum, that can lead to increase LDL cholesterol level in the blood. Sea grapes extract (Caulerpa racemosa) contains antioxidant polyphenolic group that can reduce MTP and ACAT-2 in the body that can decrease LDL cholesterol level in the blood.The purpose of this study is to know the effect of sea grapes extract on decreasing LDL cholesterol of white male Wistar rats (Rattus norvegicus) fed with high fat diet. Method: 24 white male Wistar rats, that divided into 3 groups: 1) group of rats fed with standard diet for 28 days; 2) group of rats fed with high fat diet for 28 days; 3) group of rats fed with high fat diet for 28 days and given 10 gram/kg body weight/day of sea grapes extract on 15th-28th days. Then the blood LDL cholesterol level measured on the 29th day. Result : One-Way ANOVA Test showed there was significant difference (p=0.004) of LDL level between the group of rats fed with standard diet (12.37 mg/dl) compared to group of rats fed with high fat diet (17.87 mg/dl). There was significant difference (p=0.001) of LDL level between the group of rats fed with high fat diet (17.87 mg/dl) compared to group of rats fed with high fat diet and sea grapes extract (10.12 mg/dl). Conclusion: high fat diet significantly increase blood LDL cholesterol level and sea grapes extract (Caulerpa racemosa) significantly decrease blood LDL cholesterol level
The Effect of Black Cumin Seed (Nigella sativa l.) Extract to Alloxan Induces Pancreatic Tissue Catalase Enzyme Activity of Male Wistar Rats (Rattus norvegicus) Qonita Achmad; Irmawati Dikman; Sulistiana Prabowo
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 7, No 1 (2018): edisi Maret 2018
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.801 KB) | DOI: 10.30742/jikw.v7i1.314

Abstract

Alloxan causes the formation of reactive oxygen through the reduction process of alloxan (dialuric acid) in the β cells of pancreas. Free radicals cause damage  and decrease catalase enzyme activity. Black cumin seeds extract which contain carotenoids, thymoquinone, carvacrol, 4-terpineol, t-anethole, and tert-butylhydroquinone (TBHQ) has antioxidant activity that is expected to inhibit reactive oxygen species (ROS) that is expected to increase pancreatic catalase activity. This study used 24 male Wistar rats, divided into 3 groups:1)group of rats fed with standard food, 2)group of rats induced by alloxan 125mg/kg /BB intraperitoneally on day 1, 3)groups of rats induced by alloxan 125mg/kg/BB intraperitoneally on day 1 and were given by black cumin seed extract 2500mg/kg/BB orally on day 4 until 17. On the 18th day of all rats were sacrificed and pancreatic tissue catalase activity were measured by the spectrophotometric method. The results of One-Way ANOVA showed significant difference (p=0.003) pancreatic tissue catalase activity of groups of rats fed with standard food (1696,96U/g±414,10U/g) compared with group of rats induced by alloxan (1287,58U/g±88,45U/g). There was significant difference (p=0.001) of pancreatic tissue catalase activity of groups of rats induced by alloxan (1287,58U/g±88,45U/g) with a group of rats induced by alloxan and given with black cumin seed extract (1764,83U/g±199,69U/g). The conclusion of this study showed that the administration of alloxan significantly decreased pancreatic tissue catalase activity of rat and black cumin seed extract significantly increased catalase activity because cumin extract has antioxidant effects.
Co-Authors ANTHONY ANUGERAH JOSHUA FRANSISKA CHRISTELA Irmawati Dikman Lybelary Dewi Satrianawaty Qonita Achmad Riami RONALDO LAU Tri Martini Sumarno YUNITA SURYA PRATIWI