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All Journal Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Sains dan Kesehatan Indonesian Journal of Interdisciplinary Research in Science and Technology (MARCOPOLO)
Suparno Putera Makkadafi
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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PENGARUH VARIASI WAKTU PENYIMPANAN TERHADAP STABILITAS REAGEN KERJA PADA HASIL PEMERIKSAAN KADAR KREATININ Silvi Marshela; Suryanata Kesuma; Suparno Putera Makkadafi
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3809

Abstract

Kidney is one of the organs of the body whose role is very important to maintain the stability of volume, osmolarity of extracellular fluid, and electrolyte composition. One of the parameters to determine kidney function that is usually done is creatinine examination. Preparation of working reagents is very important for accurate results in an examination. The purpose of this study was to determine the effect of storage on the stability of working reagents on creatinine examination. This type of research is a pseudo-experiment, to determine the difference between working reagents that are directly used with working reagents that are stored for several days. The research variables are the length of storage of work reagents, creatinine levels and storage temperature. The sample used in this study was normal creatinine control. The results were analyzed using Microsoft Excel and using the Kruskal Wallis Non Parametric test. The results of the study showed that the average reagent immediately and 5 days was 0.8 mg/dl and at 8 days 1.2 mg/dl, the accuracy obtained was -3.2% and 0.4%, the precision value obtained was 35.1% and 31.0%, in statistical tests significant results <0.05, meaning that there are differences in the results of examinations using working reagents that are used immediately and those stored for 5 and 8 days.
PENGARUH VARIASI WAKTU PENDINGINAN CARBOL FUCHSIN TERHADAP HASIL PEMERIKSAAN BASIL TAHAN ASAM Amelia Maulida; Tiara Dini Harlita; Suparno Putera Makkadafi
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3906

Abstract

Microscopic examination with Ziehl Neelsen staining is one way to confirm the diagnosis of tuberculosis. Ziehl Neelsen staining has 3 reagents, namely carbol fuchsin 1%, alcohol acid 3%, and methylene blue 0.1%. Based on Standard Operating Procedures, the carbol fuchsin cooling process is carried out for 5-7 minutes. The aim of this research was to determine the effect of varying carbol fuchsin cooling times of 6 minutes, 8 minutes, 10 minutes and 12 minutes on the results of BTA microscopic examination. This type of research is an experiment with a completely randomized design (CRD). The samples used were BTA (+3) sputum specimens from RSUD. Abdoel Wahab Sjahranie 3 pots. The quality assessment of BTA preparations was carried out by the East Kalimantan Provincial Health Laboratory. BTA preparations were given 4 treatments varying in carbol fuchsin cooling time, namely 6 minutes, 8 minutes, 10 minutes and 12 minutes, then repeated 6 times and analyzed using the Chi-Squared test. The results of this study were that all BTA preparations that were treated with variations in carbol fuchsin cooling time were of good quality based on 6 criteria for good preparations with an average scoring of 100% in the 6 minute treatment, 98.3% in the 8 minute treatment, 96.6% in the 8 minute treatment. 10 minutes and 95% in the 12 minute treatment. Based on the results of the Chi-Squared test analysis of cleanliness criteria, a p value of 0.000 was obtained, indicating that there was no significant influence between variations in carbol fuchsin cooling time on the results of BTA microscopic examination. Thus, during the cooling stage, carbol fuchsin can be used for a duration of 5-7 minutes to produce a good quality preparation.
Gambaran Jamur Candida Sp Pada Handuk Anak Kos Pagayang, Rektoviana Andi Randa; Suparno Putera Makkadafi; Nurul Anggrieni
Jurnal Sains & Kesehatan Vol 3 No 2 (2024): Jurnal Sains dan Kesehatan
Publisher : Politeknik Baubau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57151/jsika.v3i2.421

Abstract

Towels are a necessity for everyone to dry their body and hair. Moist towels without sunlight cause mold and bacteria growth. Candida sp is an infection of the skin that usually develops on nails and body parts. This study aims to identify and determine the percentage of Candida sp fungi on towels of boarding children in Loa Jananan Ilir District conducted in March 2024, this type of research is descriptive with a Cross Sectional design. The number of samples in this study with 44 samples taken randomly Spada towels boarding children. From the identification obtained 47% of boarding towels contained Candida sp. It is necessary to pay more attention to personal hygiene, especially for boarding school children, so that health problems, especially those caused by the Candida Sp fungus, can be minimized.
Total Plate Count of Germs on Pear Surface Swabs During Washing with Fruit Washing Soap Defitri Afira; Suparno Putera Makkadafi; Nurul Anggraeni
Indonesian Journal of Interdisciplinary Research in Science and Technology Vol. 2 No. 8 (2024): August 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/marcopolo.v2i8.10949

Abstract

The surface of the fruit is the first contamination of microorganisms on fruit with varying types and amounts. As many as 20% of cases of food poisoning caused by Salmonella typhi were recorded as a result of the consumption of fresh ready-to-eat products such as fruits. Washing the fruits that will be consumed is a good step, so a fruit washing soap product is used that can clean the fruits from these bacteria. Nonionic surfactants are cleaning agents found in fruit washing soaps that can remove bacteria on surfaces. The purpose of this study was to determine the difference in the total plate number of germs on the surface of pears when washing with fruit washing soap. The research method used in this research is descriptive method. The population is 50 soaps taken from 5 shops in the Harapan Baru market in Samarinda with different soap brands. The sample taken is 10% of the total population, namely 5 soaps. The sampling technique used purposive sampling. The study was carried out in July 2022. The method of examination used the examination of the TPC (Total Plate Count) with the pour plate method using PCA (Plate Count Agar) media. The data analysis used is univariate analysis which aims to explain the characteristics of each research variable by looking at the frequency distribution. The results showed that the number of bacteria before washing with fruit washing soap ranged from 4.4 x 100 CFU/cm2 to 1.4 x 101 CFU/cm2. Meanwhile, the number of bacteria after washing with fruit washing soap ranged from 0.4 x 100 CFU/cm2 to 3.4 x 100 CFU/cm2. The conclusion of this study is that there are differences in the number of total bacterial plate count on the surface of pears when washing with fruit washing soap.
Co-Authors Amelia Maulida Anggrieni, Nurul Defitri Afira Kesuma, Suryanata Nurul Anggraeni Pagayang, Rektoviana Andi Randa Silvi Marshela Tiara Dini Harlita