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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Meditory : The Journal of Medical Laboratory Klinikal Sains (Jurnal Analis Kesehatan) Jambura Journal of Health Sciences and Research Jurnal Medika Malahayati Husada Mahakam: Jurnal Kesehatan JURNAL ANALIS LABORATORIUM MEDIK Borneo Journal of Medical Laboratory Technology Medicra (Journal of Medical Laboratory Science/Technology) Health Care : Jurnal Kesehatan Jurnal Kesehatan dan Pembangunan Jurnal Kesehatan Tambusai Jurnal Sains dan Kesehatan Borneo Journal of Science and Mathematics Education Journal of Sustainability Science and Technology Gema Kesehatan

Kesuma, Suryanata

Poltekkes Kemenkes Kalimantan Timur

Author-ID : 1884346

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Energy Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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Evaluasi Analitik Hematology Analyzer Diatron Abacus 3 Pada Parameter Hematologi Rutin Di Laboratorium Hematologi Poltekkes Kemenkes Kalimantan Timur Suryanata Kesuma; Mahdiah Syumarliyanty; Agus Rudi Hartono
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.6467

Entering the era of globalization, manual tools in clinical laboratories have been replaced by full automatic devices. One of them is the Diatron Abacus Hematology analyzer 3. A relatively new hematology analyzer is required for analytical evaluation. Analytical evaluation is an evaluation of Diatron Abacus 3 on Sysmex KX 21 as a standard in RSUD I.A Moeis Samarinda, and it is very important to do this to assess the performance of the tool. Analytical evaluation is done by determining the value of accuracy, precision, and total error and linearity of measurement results from routine hematological examination parameters, which are erythrocytes, leukocytes, platelets, hemoglobin, and hematocrit. To determine the results of the hematology analyzer evaluation of Abacus 3 Diatron Analyzers on Routine Hematology Parameters in the Hematology Laboratory of the Health Ministry of Health, East Kalimantan. This type of research is observational descriptive, using a total sampling technique, and a sample of 40 complete K3EDTA blood specimens. Data processing using Microsoft Excel and SPSS 20 applications, analyzed using descriptive statistics. The observations were still included in the criteria for acceptance, accuracy or inaccuracy (d%) in erythrocytes 1.8%, leukocytes 8.0%, platelets -5.3%, hemoglobin 2.3% and hematocrit -1.7%; Precision or impression (CV%) in erythrocytes 4.2%, leukocytes 11.1%, platelets 6%, hemoglobin 3.9% and hematocrit 4.5%; Total errors in erythrocytes were 8.7%, leukocytes 17.9%, platelets 23.6%, hemoglobin 8.8% and hematocrit 9.1; Linearity of the measurement results against the routine hematological cell count values performed using Abacus 3 and Sysmex KX 21 has a positive relationship. Acceptance values are still included in the LOA on all parameters examined and still meet the criteria; the accuracy/bias value is smaller than the true value of the parameter being examined, except for leukocytes, which is greater; the precision on the five parameters is greater than the CV% Abacus 3 insert kit; The total error obtained by the TE value is greater than the TEA in the parameters examined, except for smaller platelets; Linearity of the measurement results against the calculated hematology cell routine values performed using Abacus 3 and Sysmex KX 21 have a positive relationship, meaning an increase in measurement values using Abacus 3 is followed by an increase in measurement values using Sysmex KX 21.

UJI DIAGNOSTIK GENE XPERT MTB/RIF PADA PEMERIKSAAN MYCOBACTERIUM TUBERCULOSIS DI RSUD R. SYAMSUDIN SH KOTA SUKABUMI Suryanata Kesuma; Tatang Abdullah
Husada Mahakam Vol 10 No 2 (2020): Nopember 2020
Publisher : Poltekkes Kemenkes Kalimantan Timur (URL: http://poltekkes-kaltim.ac.id/)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35963/hmjk.v10i2.214

Mycobacterium Tuberculosis infection is one of the endemic infectious diseases in Indonesia. The Mycobacterium Tuberculosis examination method in Indonesia uses conventional microscopic techniques. However, currently, a rapid molecular examination method has been developed, which is Gene Xpert MTB / RIF. The Clinical Laboratory of Syamsudin SH Sukabumi Regional Hospital is one of the hospitals that received a Gene Xpert MTB / RIF tool grant from the government. In the course of its use, diagnostic tests have not been conducted on this tools. The purpose of this study was diagnostic testing of Gene Xpert MTB / RIF for the examination of Mycobacterium Tuberculosis infection with sputum specimens. Diagnostic test values are calculated by making a 2x2 table. The comparison method in this study is conventional microscopic. Gene Xpert MTB / RIF the Clinical Laboratory of Syamsudin SH Sukabumi Regional Hospital, those are Sensitivity 85.3%, Specificity 100%, Positive Prediction Value 100% and Negative Prediction Value 92.3%. The conclusion of this research is the Gene Xpert MTB / RIF of The Clinical Laboratory of Syamsudin SH Sukabumi Regional Hospital is very high so that it can be used for screening and diagnostic Mycobacterium Tuberculosis infections.

EVALUASI ANALITIK POCT METODE GLUCOSE DEHYDROGENASE PARAMETER GLUKOSA PADA SPESEIMEN SERUM DAN PLASMA EDTA Suryanata Kesuma; Didi Irwadi; Nia Ardelia
Meditory : The Journal of Medical Laboratory Vol 9, No 1 (2021): Meditory, Volume 9, No 1, Juni Tahun 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i1.1293

Diabetes is a non-communicable disease that can be prevented and detected as early as possible by routine glucose checks. Point Of Care Testing (POCT) as a diabetes test screening tool is widely used by health care institutions and is even used independently by the wider community. Blood glucose can be measured by serum, plasma, and whole blood specimens. The standard specimen for glucose measurement using POCT is whole blood. Based on the author's survey, the use of the POCT tool in several health care institutions was not following the insert of the tool kit, one of which was the type of specimen used. Many laboratories use EDTA serum and plasma specimens for glucose testing using the POCT device. There are many methods used in the POCT tool, one of these methods is the glucose dehydrogenase method. Therefore analytic evaluation is necessary to ensure the accuracy and accuracy of laboratory examination results in glucose examination. This type of research is the analytical method with a comparative design. The data was collected using the total sampling method. The data is reported using Microsoft Excel and SPSS applications. The number of samples for analytic evaluation was 40 samples. Based on the results of tests performed in duplicate, there were clinical differences in glucose values using serum and plasma EDTA samples so that the use of these samples was not suitable for use for diagnostic purposes using the POCT glucose dehydrogenase method. There was a statistical difference in serum glucose and plasma EDTA against whole blood that was examined using the POCT glucose dehydrogenase method with a correlation value (R) of 0.9722 and 0.9695.

Gambaran Fraksi Hemoglobin Penderita Talasemia Menggunakan Metode Elektroforesis Kapiler Suryanata Kesuma; Elita Octavia
Meditory : The Journal of Medical Laboratory Vol 6, No 2 (2018): Meditory, volume 6, nomer 2, Desember 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Thalassemia is a hereditary blood disease that is found in Indonesia. One method of examining modern thalassemia is the examination of hemoglobin fraction using the Capillary Electrophoresis. This method has a high level of accuracy and precision for quantification of hemoglobin variants.The purpose of this study was to determine the hemoglobin fraction in thalassemia patients using the Capillary Electrophoresis method. This type of research is descriptive research. The sample of this study was 3 blood specimens from thalassemia sufferers.The results showed that the first specimen, age 4 years had HbA levels of 59.9%, HbA2 levels of 4.3%, HbF levels of 14.7% and HbE levels of 21.1%. In the second specimen, 8 years of age had 88.7% HbA, 2.5% HbA2, 3.2% HbF and 5.6% HbE. In the third specimen, the age of 13 years had HbA levels of 93.8%, HbA2 levels of 4.9% and HbF levels of 1.3%.The conclusion of this study was the first specimen, HbA levels decreased, HbA2 levels increased and HbF levels increased and hemoglobin variants were found, namely HbE. In the second specimen, HbA levels decreased from the normal range, HbA2 levels were in the normal range and HbF levels increased and hemoglobin variants were found, namely HbE. In the third specimen, HbA levels decreased from the normal range, HbA2 levels and HbF levels increased, but no hemoglobin variants were found in this specimen.

Evaluasi Penggunaan Biosaliva Dalam Deteksi Sars-Cov-2 Metode RT-PCR Suryanata Kesuma; Suparno Putera Makkadafi
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.11280

SARS CoV-2 infection, which has affected the world since late 2019, can cause serious lower respiratory tract infections that may be fatal in some patients. This infection causes the disease Covid-19. The diagnosis of SARS-CoV-2 infection was carried out by NAAT (Nucleic Acid Amplification Test) such as RT-PCR examination. The sample needed for the identification of SARS-COV-2 is a nasopharyngeal/oropharyngeal swab. Nasopharyngeal/oropharyngeal swab sampling requires trained personnel. Taking a nasopharyngeal/oropharyngeal swab is invasive, causing discomfort in its implementation. The convenience of sampling specimens can be an alternative option for the identification of SARS-CoV-2, such as with newly developed biosaliva specimens. The use of this biosaliva sample can be a practical option in the examination of the identification of SARS-CoV-2. However, the use of these specimens needs to be evaluated first because of the possible relationship with clinical findings and so that the results of the SARS-CoV-2 examination are valid and reliable. The purpose of this study was to evaluate the use of biosaliva specimens to detect SARS-CoV-2 infection with the RT-PCR method. Evaluation of the use of biosaliva in the detection of SARS-COV-2 RT-PCR method with paired T test and diagnostic test with the gold standard using nasopharyngeal/oropharyngeal swabs. The target genes for the detection of SARS-CoV-2 are the RdRp gene and the E gene with control of the HRP gene. RT-PCR was carried out with 40 cycles and Tm 62 °C. The results of this study are Sig. (2-tailed) paired T test was 0.106, sensitivity was 64.86% and specificity was 90.92%. The conclusion of this study is that there is no statistical difference in the results of the SARS-CoV-2 RT-PCR method between the use of biosaliva specimens and nasopharyngeal/oropharyngeal swabs, and the evaluation results show that reliable biosaliva specimens are used as samples in the examination of SARS-COV-2 infection.

Uji Diagnosis NS1, IgG dan IgM Dengue Metode Immunokromatografi dan Elisa Suryanata Kesuma
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3374

Dengue fever is a disease caused by the dengue virus (DENV). Detection of NS1 antigen can be useful for early confirmation of DENV infection. NS1 antigen can be detected from the first day of illness and lasts up to 9-10 days. Other supporting examinations are dengue virus IgG and IgM examinations. The purpose of this study was to review the sensitivity and specificity of the dengue virus NS1, IgG, and IgM tests through literature testing. This type of research is descriptive. The literature used is scientific journals which are the top journals searched using the google search engine and contains complete information and data related to the sensitivity and specificity of NS1, IgG, and IgM Dengue Virus examinations. Based on the review, it was found that the sensitivity of the NS1 antigen in the Rapid Immunochromatographic method was in the range of 48%-92% and specificity was 73%-100%, in the ELISA method the sensitivity range was 42%-84% and specificity was 89%-99%. In the examination of IgG and IgM antibodies using the Rapid Immunochromatography method, the sensitivity ranges from 6%-73% and specificity 36%-100%. Examination of IgG and IgM antibodies using the ELISA method obtained sensitivity in the range of 6%-58% and specificity 56%-100%. The results of the review conducted showed that the sensitivity of the NS1, IgG, and IgM tests for Dengue Virus with the Rapid Immunochromatography method was better than the ELISA method, but the specificity for the NS1, IgG, and IgM tests for Dengue Virus ELISA method was better than the Rapid Immunochromatography method. In addition, the Rapid Immunochromatography method of NS1 antigen examination showed higher accuracy than IgG and IgM antibodies in screening for dengue virus infection.

HUBUNGAN KADAR KREATININ DAN MIKROALBUMIN PADA PASIEN DIABETES MELLITUS TIDAK TERKONTROL DI KLINIK MEDIA FARMA SAMARINDA Suryanata Kesuma; Nurul Anggrieni; Nurul Alidasyah
Klinikal Sains : Jurnal Analis Kesehatan Vol 10 No 2 (2022): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v10i2.2695

Diabetes Mellitus (DM) is a non-communicable disease but is a fairly large health problem in Indonesia today. The HbA1C examination is the gold standard in measuring glycemic levels, while the examination of creatinine and microalbumin levels is one of the parameters used to assess kidney function or diabetic nephropathy. This study aims to determine the relationship between creatinine levels and microalbumin levels in patients with uncontrolled diabetes mellitus at the Media Farma Samarinda Clinic. This study used a cross-sectional design using the Spearman correlation test. The samples in this study were 107 samples with HbA1C levels > 7%. The sampling technique was carried out using quota sampling. The variables measured were levels of HbA1C, creatinine, and microalbumin. The results of this study indicate that the value of p = (0.589) > = (0.05) in the correlation of HbA1C > 7% with creatinine and the value of p = (0.085) > = (0.05) in the correlation of HbA1C > 7% with microalbumin and the value of p = (0.032) > (0.05) on the correlation of creatinine with microalbumin so that H0 is accepted and Ha is rejected. Based on the results of this study, it can be concluded that there is no relationship between creatinine and microalbumin levels in patients with uncontrolled diabetes mellitus at the Media Farma Samarinda Clinic.

Liver histology of Wistar rats (Rattus norvegicus) following oral administration of 50% ethanol Liah Kodariah; Rahmad Rezaldy; Suryanata Kesuma; Farhan Baehaki
Journal of Sustainability Science and Technology Vol. 2 No. 1 (2022): Journal of Sustainability Science and Technology (JOSST)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v2i1.21

Ethanol, also known as ethyl alcohol, pure alcohol, and alcohol, is a toxic, volatile, flammable, and colourless liquid. Alcohol is the most commonly consumed alcoholic beverage in everyday life. EtOH is a common abbreviation for ethanol, where "Et" stands for the ethyl group (C2H5). Sugar fermentation to ethanol is one of the earliest organic reactions ever performed by humans; ethanol consumption has also been known for a very long time. This study aims to determine the histology of the liver in Wistar rats (Rattus norvegicus) by orally administering ethanol at a concentration of 50 percent. The method used in this study was an experimental study by looking at the histology of rat liver. Rats were divided into two groups, with seven rats each. Group 1 was given 50% ethanol orally for seven days, and group 2, as a control, was only given orally with distilled water. After seven days, the rats were slaughtered, and their livers were extracted for further histological preparations. In the histology results of group 1, the histological images of the livers of the rats P1, P2, P3, P4, P5, P6 and P7 were abnormal or damaged. In the presence of necrotic cells, oral administration of 50 percent alcohol causes damage to hepatocyte cells, as determined by the study's findings. However, in general, hepatocyte cell damage in the liver produces a score of 1.7, which indicates a change leading to cell damage.

Evaluasi Kadar Kreatinin dan Mikroalbumin dengan HbA1C Suryanata Kesuma; Sresta Azahra; Amalia Diah Suci Rahmah
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.4790

Diabetes Mellitus (DM) is a metabolic disease caused by high blood glucose levels (hyperglycemia). Hyperglycemia is caused due to lack of insulin secretion in the body. Glycemic control in diabetic patients is seen in HbA1C levels. One of the complications of DM is diabetic nephropathy. Renal function abnormalities associated with diabetic nephropathy are indicated by increased levels of creatinine and microalbumin. This shows that it is necessary to monitor kidney function in DM. Monitoring DM patients with HbA1C levels <6.5% is desirable, with these levels indicating that glycemic control is good. This study aimed to determine the relationship between creatinine and microalbumin levels with HbA1C <6.5% in Diabetes Mellitus patients in Samarinda. This type of research is analytic with a cross-sectional design. Spearman correlation statistical test. The sample of this study was 107 DM patients with HbA1C levels <6.5% who did medical checkups at the Media Farma Clinic, Samarinda. Sampling is done by Total Sampling. HbA1C examination method with turbidimetry. Method of checking creatinine by Jaffe reaction. Method of examination of microalbumin with immunoturbidimetry. All parameters are quality controlled before measurement. The measurement results were validated by a clinical pathologist. The results showed that the correlation of creatinine with HbA1C <6.5% obtained p value = (0.826), microalbumin with HbA1C <6.5% obtained p value = (0.146), and creatinine with microalbumin obtained p value = (0.082). All these results indicate that H0 is accepted and Ha is rejected. This study concludes no significant relationship exists between creatinine and microalbumin values with HbA1C levels <6.5% in Diabetes Mellitus in Samarinda, especially Media Farma Clinic.

UJI DAYA HAMBAT CACING TANAH (Lumbricus rubellus) TERHADAP PERTUMBUHAN BAKTERI Salmonella typhi SECARA IN VITRO Suryanata Kesuma; Lamri Lamri; Marezha Aulia Putri Sugiarsono
Husada Mahakam Vol 12 No 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Kalimantan Timur (URL: http://poltekkes-kaltim.ac.id/)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35963/hmjk.v12i2.324

One of the pathogenic microbes that often infect humans is Salmonella typhi which causes typhoid fever. Antibiotics are the main choice in treating typhoid fever. Herbal ingredients are very safe for humans because they contain active compounds as effective antiseptics. In the modern world today, the active compound lumbricin in earthworms (Lumbricus rubellus) is used as a medicinal ingredient. The purpose of the study was to determine the diameter of the inhibition zone and to determine the effectiveness of the inhibition of earthworm powder (Lumbricus rubellus) with concentrations of 25%, 50%, and 100% against the growth of Salmonella typhi bacteria. This type of research is a laboratory experiment. Earthworm (Lumbricus rubellus) is processed into powder by roasting technique, then made in the form of a medicinal disc. Earthworm powder was tested for sensitivity by disc diffusion method in concentrations of 25%, 50%, and 100% against the growth of Salmonella typhi, which was cultured in Muller Hinton Agar media with a clear zone formed for 24 hours. The study results were carried out with seven repetitions at a concentration of 25% no antibacterial power and antibacterial effectiveness. However, at concentrations of 50% and 100%, the average diameter of the inhibition zones was 0.21 mm and 0.92 mm, and the antibacterial efficacy was 0.7% and 3.4%, respectively. This study concludes is that at a minimum concentration of 50%, antibacterial power and effectiveness were obtained.

Co-Authors Adi Firmansyah Agus Rudi Hartono Agus Rudi Hartono Amalia Diah Suci Rahmah Anggrieni, Nurul Armiah Gita Anggraini DESI WAHYUNI Didi Irwadi Dwi Setiyo Prihandono Dwi Wulan Sari Effie Raissa Widyadari Eka Farpina Eka Farpina, Eka Farpina Elita Octavia Farhan Baehaki I Gede Andika Sukarya Irawiraman, Hadi Lamri Lamri Lamri Lamri Liah Kodariah Mahdiah Syumarliyanty Marezha Aulia Putri Sugiarsono Maulida Maulida Julia Saputri Nanda Nur Adha Sari Febriyanti Nashi Widodo Nia Ardelia Nursalinda Kusumawati Nurul Alidasyah Nurul Hikmah Oktavia Rahayu Adianingsih Patricia Aleksandra Putri Sekar Sari Rahmad Rezaldy Silvi Marshela Siti Rodiyah Sresta Azahra Sri Winarsih Sultan Sultan Sultan Sultan Suparno Putera Makkadafi Suparno Putera Makkadafi Supri Hartini Tatang Abdullah VALENTINA YURINA

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