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Perbandingan Efektivitas Ekstrak Etanol Daun Pepaya (Carica papaya L.) dan Ekstrak Etanol Daun Kemangi (Ocimum x africanum Lour.) dalam Menurunkan Kadar Glukosa Darah Mencit (Mus musculus L.) Hiperglikemia Antika Febiola Utami; Sutyarso Sutyarso; Sri Wahyuningsih; Nuning Nurcahyani
Buletin Anatomi dan Fisiologi Volume 7, Nomor 2, Tahun 2022
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.7.2.2022.91-100

Abstract

Hiperglikemia terjadi ketika keadaan kadar glukosa darah melebihi batas normal. Daun pepaya (Carica papaya L.) dan daun kemangi (Ocimum x africanum Lour.) dilaporkan mengandung senyawa yang berperan sebagai antihiperglikemia. Penelitian ini dilakukan karena dari beberapa khasus di Indonesia, penderita hiperglikemia cukup tinggi maka penelitian ini perlu dilakukan untuk memberikan informasi mengenai efektivias dari suatu tanaman herbal dalam menangai hiperglikemia.Tujuan penelitian untuk mengetahui perbandingan efektivitas pemberian ekstrak etanol daun pepaya dan daun kemangi dalam menurunkan kadar glukosa darah mencit hiperglikemia. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 25 ekor mencit dibagi dalam 5 kelompok perlakuan dan 5 kali ulangan. Kelompok perlakuan diantaranya, K(N) (tanpa perlakuan), K(-) (diinduksi aloksan ), K(+) (diinduksi aloksan dan diberi perlakuan dengan glibenklamid), P1 perlakuan 1 (diinduksi aloksan dan diberi perlakuan dengan ekstrak etanol daun pepaya), P2 perlakuan 2 (diinduksi aloksan dan diberi perlakuan dengan ekstrak etanol daun kemangi). Parameter yang diukur dalam penelitian ini yaitu berat badan dan kadar glukosa darah mencit. Data yang diperoleh dianalisis dengan one way ANOVA dan uji lanjut LSD pada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian bahan uji mampu menurunkan kadar glukosa darah mencit dengan persentase penurunan lebih efektif pada P2 sebesar 60,15% sedangkan P1 sebesar 53,32% . Hyperglycemia occurs when blood glucose levels exceed normal limits. Papaya (Carica papaya L.) and basil (Ocimum x africanum Lour.) leaves are reported to contain compounds that act as antihyperglycemic agents. This study was conducted because in some cases in Indonesia, people with hyperglycemia are quite high, so this study needs to be carried out to provide information about the effectiveness of an herbal plant in dealing with hyperglycemia. hyperglycemic mice blood. This study used a completely randomized design (CRD) with 25 mice divided into 5 treatment groups and 5 replications. The treatment groups included, K(N) (without treatment), K(-) (alloxan induced), K(+) (alloxan induced and treated with glibenclamide), P1 treatment 1 (alloxan induced and treated with ethanol extract of papaya leaves ), P2 treatment 2 (alloxan induced and treated with ethanol extract of basil leaves). The parameters measured in this study were body weight and blood glucose levels of mice. The data obtained were analyzed by one way ANOVA and LSD further test at the 5% level. The results showed that the administration of the test material was able to reduce blood glucose levels in mice with a more effective percentage reduction in P2 of 60.15% while P1 of 53.32%. 
Induction of Rhizoctonia resistance to odontoglossum ringspot virus (ORSV) infection on orchid leaf organ viability Eka Nuraini Tohari; Mahfut Mahfut; Tundjung Tripeni Handayani; Sri Wahyuningsih
Journal of Sustainability Science and Technology Vol. 1 No. 2 (2021)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v1i2.4

Abstract

Orchid plants are in great demand by the public as ornamental plants that have economic value and the beauty of attractive flower shapes and colors, especially Phalaenopsis and Dendrobium. Related to this, conservation efforts are needed through cultivation. However, these efforts are often hampered by viral infections. One of the viruses that trigger inhibition of orchid cultivation that often appears is ORSV. Control efforts that are relatively safe and efficient can utilize mycorrhizae in orchids which play a role in increasing the absorption of plant nutrients. The use of Phalaenopsis and Dendrobium with the induction of Rhizoctonia sp. against ORSV is a very important alternative biofertilizer and biocontrol agent. The objectives of this study were 1) to determine the effectiveness of orchids induced by Rhizoctonia resistance, 2) to determine the viability of orchid leaf organs to ORSV infection, 3) to determine the viability of orchid root organs resulting from Rhizoctonia resistance to ORSV infection. This study was conducted in January 2021 – March 2021 at the Botanical 2 Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. This study employed a completely randomized factorial with 6 treatments and 4 replications. The variable observed in this study was the effectiveness of mycorrhizae, leaf and root length, number of leaves and roots, and leaf width. The data obtained were homogenized using Levene's test and then analyzed by ANOVA and Tukey's further test at the 5% level. The results showed that the effectiveness of mycorrhizae occurred on the 3rd day of incubation. In leaf organs, Phalaenopsis amabilis showed an increased response and Dendrobium discolour showed a decreased response in leaf length, width, and number. Meanwhile, in the root organ, Dendrobium discolour showed an increased response and Phalaenopsis amabilis showed a decreased response in root length and number of roots.
Various ORSV infection symptoms on ceratorhiza induction in orchid plants Mitha Valentina Treesya Panjaitan; Mahfut Mahfut; Tundjung Tripeni Handayani; Sri Wahyuningsih
Journal of Sustainability Science and Technology Vol. 1 No. 2 (2021)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v1i2.5

Abstract

Several types of orchids that are in great demand and cultivated by various groups are Phalaenopsis sp. and Dendrobium sp. Mycorrhizae are needed by orchid plants to complete their life cycle. This is because mycorrhizae are capable of symbiosis with the root system of the host plant so that the plant's capacity to absorb elements will increase. This study aims to determine the extent to which Ceratorhiza induction on Phalaenopsis amabilis and Dendrobium discolour orchids can suppress the symptoms of Odontoglossum ringspot virus (ORSV) infection. The method used in this study was a factorial completely randomized design (CRD) with 2 factors and 4 replications. The first factor is the type of orchid used, namely Phalaenopsis amabilis (A1) and Dendrobium discolour (A2), while the second factor is the type of administration of mycorrhizae, viruses, and mycorrhizal viruses. Observation of disease symptoms is done by looking at the symptoms that appear on leaves that have been infected with ORSV. The results showed that the symptoms that appeared in Phalaenopsis amabilis were in the form of mosaic, necrosis, leaf malformation, while in Dendrobium discolour were mosaic and necrotic. Ceratorhiza was not able to suppress the symptoms of disease infection because based on the results obtained; the treatment on orchids inoculated with mycorrhizae and viruses showed more severe symptoms compared to the treatment only inoculated with viruses increased response and Dendrobium discolours showed a decreased response in leaf length, width, and number. Meanwhile, in the root organ, Dendrobium discolour showed an increased response and Phalaenopsis amabilis showed a decreased response in root length and number of roots.
KARAKTER PENCIRI MORFOLOGI TANAMAN UBI JALAR (Ipomoea batatas) (L.) Lam. DAN KEANEKARAGAMANNYA DI LAMPUNG Yulianty Yulianty; Sri Wahyuningsih; Eti Ernawiati
Jurnal Ilmiah Hijau Cendekia Vol 8 No 2 (2023): Jurnal Ilmiah Hijau Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/hijau.v8i2.3318

Abstract

Tanaman ubi jalar merupakan tanaman yang memiliki umbi batang. Ada beberapa macam ubi jalar berdasarkan warna umbinya, ada yang putih, kuning dan ungu. Khusus untuk ubi ungu mempunyai kandungan antosianin sehingga berwarna ungu. Pemilihan ubi jalar ungu disebabkan karena memiliki manfaat untuk kesehatan. Untuk mengetahui ciri dari tanaman ubi jalar dapat dilakukan dengan mengamati morfologi dan anatomi baik umbi maupun daunnya. Perlu dilakukan penciri lainnya sehingga memudahkan untuk mengenal kultivar yang ada dan ditemukan di Lampung. Hasil pengamatan morfologi untuk ubi jalar yang telah ditemukan memiliki karakter daun yang berbeda-beda, karakter lainnya adalah warna dan bentuk ubi jalar. Ada yang berwarna putih, oranye muda dan unguyang diperoleh dalam penelitian.