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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Kedokteran Gigi Terpadu Jurnal Abdimas Kesehatan Terpadu Sonde (Sound of Dentistry)
Didi Nugroho Santosa
Pendidikan Dokter Gigi, Fakultas Kedokteran Gigi, Universitas Trisakti, Jakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Efektivitas Kombinasi Natrium Hipoklorit dengan Lansoprazole dalam Menghambat Enterococcus faecalis dan Eschericia coli Gabriella Geralda Santjoko; Didi Nugroho Santosa
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 1 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (740.745 KB)

Abstract

Background: Sodium hypochloride (NaOCl), which is the gold standard for irrigation solutions, has low bactericidal activity against Enterococcus faecalis, the main cause of root canal treatments failure. This resistance due to its proton pump, which can also be found in Escherichia coli. This research was conducted using a combination of NaOCl with lansoprazole, the latest generation proton pump inhibitor. Objective: To determine the antibacterial effect of the combination of NaOCl with various concentrations of lansoprazole against E. faecalis and E. coli. Method: Laboratory experimental research in vitro with the diffusion method which was carried out in the MiCORE laboratory, Faculty of Dentistry, Universitas Trisakti. The independent variables were 5.25% NaOCl combination of 4% and 8.5% lansoprazole, 8.5% lansoprazole, 5.25% NaOCl combination of omeprazole 8.5%, 5.25% NaOCl and aquadest. Results: There was no inhibition zone of E. faecalis and E. coli in 8.5% lansoprazole, 5.25% NaOCl combination of omeprazole 8.5%, and aquadest. Inhibition zones of E. faecalis and E. coli were formed in 5.25% NaOCl combination of 4% lansoprazole, 5.25% NaOCl combination of 8.5% lansoprazole, and 5.25% NaOCl. The mean value of the inhibition zone of E. faecalis were 14,4±2,10 mm, 10,89±0,68 mm, and 25,9±1,46 mm respectively. Meanwhile, the mean value of the inhibition zone of E. coli were 19,48±2,48 mm, 10,89±0,76 mm, dan 30,22±1,10 mm. Conclusion: The antibacterial effect of 5.25% NaOCl combination of 4% and 8.5% lansoprazole was less effective than the antibacterial effect of 5.25% NaOCl.
Efek Potensiasi Antibakteri Kombinasi Sefadroksil dan Ekstrak Daun Camellia sinensis (Kajian in vitro pada Enterococcus faecalis dan Staphylococcus aureus) Didi Nugroho Santosa; Michelle
Jurnal Kedokteran Gigi Terpadu Vol. 5 No. 1 (2023): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v5i1.16776

Abstract

Background: Periapical abscess caused by tooth necrosis which are not treated. Enterococcus faecalis and Staphylococcus aureus area found in periapical abscess. Cefadroxil is one of antibiotics that choices to treat this infection. Several studies proved that C. sinensis leaves extract contains metabolite compounds such as catechins that can inhibit bacterial growth. To determine the antibacterial effect of C. sinensis leaves extract and cefadroxil combination against E. faecalis dan S. aureus growth. Methods: Laboratory experimental research with diffusion method. The research design is post test only control group design. Treatment was carried out with C. sinensis leaves extract 125 mg/mL, combination of C. sinensis leaves extract 15,625 mg/mL, 31,25 mg/mL, 62,5 mg/mL and 125 mg/mL with cefadroxil 8 μg/mL , cefadroxil 16 μg/mL as a positive control, and NaOH 0.1 N as a negative control. Results: There was none of inhibition zone in C. sinensis leaves extract 125 mg/mL and NaOH 0.1 N. The combination of C. sinensis leaves extract 15,625 mg/mL, 31,25 mg/mL, 62,5 mg/mL and 125 mg/mL and cefadroxil 8 µg/mL had an antibacterial effect. Based on Post-hoc Tukey’s HSD test, there was no significant difference between the combination and positive control. Conclusion: C. sinensis leaves extract and cefadroxil has potentially antibacterial effect on E. faecalis and S. aureus.
Efek potensiasi kombinasi sefadroksil dan ekstrak camellia sinensis terhadap pertumbuhan aggregatibacter actinomycetemcomitans Dan porphyromonas gingivalis Karyn Priscila; Didi Nugroho Santosa
Jurnal Kedokteran Gigi Terpadu Vol. 5 No. 1 (2023): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v5i1.17184

Abstract

Background: Cefadroxil, betalactam antibiotic belonging to cephalosporin class, is a drug of choice for treating periodontal infection. Previous studies reported that addition of green tea (Camellia sinensis) extract to betalactam antibiotics has shown synergistic antibacterial effect. Objective: To examine the antibacterial effect of GTE combined with cefadroxil on A. actinomycetemcomitans and P. gingivalis growth. Methods: In vitro experimental with post-test-only control design, conducted at MiCORE Laboratory, Trisakti University Faculty of Dentistry. There were 8 treatments: combination of cefadroxil 8 µg/mL with GTE 3,125 mg/mL, 6,25 mg/mL, and 12,5 mg/mL, GTE 3,125 mg/mL, 6,25 mg/mL, and 12,5 mg/mL, cefadroxil 16 µg/mL, and NaOH 0,1 N. Results: In treatment of solely GTE and NaOH 0,1, no inhibition zones were found. Meanwhile, combination of cefadroxil 8 µg/mL with GTE 3,125 mg/mL, 6,25 mg/mL, and 12,5 mg/mL, and cefadroxil 16 µg/mL inhibited both bacteria’s growth. Treatment of cefadroxil 8 µg/mL combined with GTE 3,125 mg/mL resulted in the largest inhibition zone. One-way ANOVA test showed significant difference, then post hoc test showed that combination of cefadroxil 8 µg/mL with GTE 3,125 mg/mL and 6,25 mg/mL were not significantly different from the positive control. Conclusion: Potentiation effect occurred on combination of cefadroxil 8 µg/mL with GTE 3,125 mg/mL and 6,25 mg/mL against A. actinomycetemcomitans and P. gingivalis growth. Green tea extract could increase cefadroxil antibacterial effect up to twice larger.
Penyuluhan Pentingnya Menjaga Kesehatan dan Merawat Kebersihan Gigi Tiruan di Panti Werdha Wisma Mulia Yayuk Yuliarsi; Wiwiek Poedjiastoeti; Didi Nugroho Santosa; Bernard O. Iskandar; Monica Dewi Ranggaini
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 2 No. 1 (2023): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v2i1.17264

Abstract

The impact of the Covid-19 has made the elderly's access to dental and oral health even more limited. Oral hygiene in the elderly living in nursing homes is often ignored so it falls into the poor category. The high prevalence of caries and other dental problems makes the elderly need more dental treatment. Poor oral health can lead into systemic or local infections that will affect general health, especially in the elderly who have impaired physiologic or pathologic function. Increased knowledge will improve their oral hygiene. Therefore, to increase members' knowledge and awareness to oral health, FKG Trisakti held an education to maintain their oral health and dentures. Pre and post tests are given to the participants to determine the success of the education. T test result showed increase in knowledge from participants before and after giving the material. Further training can be given to caregivers as they were there to help the elderly maintain their oral care.
Potensi Antibakteri dan Antibiofilm Kombinasi Sefadroksil dengan Ekstrak Etanol Daun Anredera cordifolia Terhadap Porphyromonas gingivalis Mulyani C; Natassya P; Didi Nugroho Santosa
SONDE (Sound of Dentistry) Vol. 10 No. 1 (2025): SONDE (Sound of Dentistry)
Publisher : Maranatha Christian University

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Abstract

Abstrak Proses inflamasi kronis oleh bakteri penyebab periodontitis dapat berdampak serius jika tidak ditangani dengan tepat. Porphyromonas gingivalis merupakan bakteri utama yang berkontribusi pada periodontitis kronis. Terapi antibiotik sering digunakan untuk mengatasi penyakit periodontal, tetapi penggunaannya yang berlebihan dan tidak tepat dapat memicu resistensi antibiotik. Kombinasi antibiotik dengan ekstrak bahan alam menjadi alternatif untuk meningkatkan efektivitas antibiotik. Daun binahong (Anredera cordifolia) memiliki sifat antibakteri karena kandungan senyawa metabolit sekundernya, seperti flavonoid, saponin, dan triterpenoid. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dan antibiofilm kombinasi sefadroksil dan ekstrak etanol daun A.cordifolia terhadap P.gingivalis. Penelitian ini berupa eksperimental laboratoris secara in vitro dengan rancangan post-test-only control group design. Penelitian diawali dengan uji fitokimia dan uji MBC sefadroksil terhadap P.gingivalis. Lalu uji antibakteri dilakukan dengan metode plate count dan uji antibiofilm dilakukan dengan metode microtiter plate biofilm assay. Sampel yang digunakan adalah kombinasi sefadroksil 1,2 µg/ml dengan ekstrak etanol daun A.cordifolia 6,25%, 12,5%, 25%, 50%, dan 100%, sefadroksil 1,2 µg/ml, sefadroksil 2,4 µg/ml sebagai kontrol positif, dan akuades sebagai kontrol negatif. Hasil MBC sefadroksil terhadap P.gingivalis adalah 2,4 µg/ml. Berdasarkan penelitian diketahui, kombinasi sefadroksil dengan ekstrak etanol daun A.cordifolia menunjukkan aktivitas antibakteri terhadap P.gingivalis pada konsentrasi 12,5% hingga 100%. Sedangkan, aktivitas antibiofilm bekerja paling optimal diamati pada masa inkubasi 3 jam. Kesimpulan penelitian ini adalah kombinasi sefadroksil dengan ekstrak etanol daun A.cordifolia berpotensimenghambat pertumbuhan bakteri dan pembentukan biofilm P.gingivalis. Kata kunci: antibakteri, antibiofilm, ekstrak etanol daun Anredera cordifolia, Porphyromonas gingivalis, sefadroksil
The Effect of Nano Chitosan Xylotrupes Gideon on Fibroblast Proliferation and Collagen Deposition in the Oral Mucosa of Rattus norvegicus Komariah Komariah; Muhammad Orliando Roeslan; Rezky Anggraeni; Didi Nugroho Santosa; Aubrey Kanya Rennata
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.23-30

Abstract

Lacerations of the oral mucosa are a common clinical problem and require an efficient healing process. The wound healing process involves the haemostasis, inflammation, proliferation, and maturation phases. During the proliferation phase, fibroblasts play an important role in migration and proliferation to produce collagen, which connects the wound's edges and provides strength and stability to the scar tissue that forms. Chitosan from the horned beetle (Xylotruphes gideon) contains active compounds that are thought to accelerate the healing process by increasing the activity of inflammatory cells and fibroblast proliferation. Physical modification into nanoparticles facilitates penetration into the mucosal layer, thereby accelerating collagen production and re-epithelialization. This study aimed to determine the effect of X. gideon nanokitosan administration on increasing fibroblast proliferation and collagen formation in vivo. The study was divided into five groups: positive control, negative control, and nano chitosan treatment at 3000, 1500, and 750µg/mL. All groups of experimental animals were previously given an injury to the right cheek mucosa with a No. 12 scalpel blade, and nano chitosan was administered topically. The increase in fibroblasts and collagen deposits was determined by HE and Masson's Trichrome staining on days 3, 7, and 14, which were observed microscopically and using ImageJ. The results showed a significant increase in fibroblasts on day 3 and collagen fibre deposition on day 14 in all treatment groups. The group with 750 ppm nano chitosan showed the best results in accelerating wound healing regarding fibroblast proliferation and collagen deposition. Conclusion: Nano chitosan X. gideon at a concentration of 750 ppm effectively accelerates wound healing in the cheek mucosa of mice by increasing fibroblast proliferation and collagen deposition.
Co-Authors Aubrey Kanya Rennata Bernard Ongki Iskandar Dewi Ranggaini Gabriella Geralda Santjoko Karyn Priscila Komariah Komariah Michelle Muhammad Orliando Roeslan Mulyani C Natassya P Rezky Anggraeni Wiwiek Poedjiastoeti, Wiwiek Yayuk Yuliarsi