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All Journal JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX Jurnal Ilmiah Sains
Billy Th Wagey
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Environmental Science Mathematics Physics

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MORPHOMETRIC ANALYSIS OF SEAGRASSES SPECIES IN NEGROS ORIENTAL Billy Th Wagey
JURNAL ILMIAH SAINS Volume 13 Nomor 2, Oktober 2013
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.603 KB) | DOI: 10.35799/jis.13.2.2013.2888

Abstract

MORPHOMETRIC ANALYSIS OF SEAGRASSES SPECIES IN NEGROS ORIENTAL ABSTRAK Studi variasi morfometrik dari 5 spesies lamun di Negros Oriental Philippines dilaksanakan pada bulan Januari sampai Maret 2011. Pengukuran variable morfologi yang diambil adalah: panjang akar,  tinggi tegakkan lamun, panjang daun terpanjang  dalam cm.  Hasil studi menunjukkan: 1) Species Lamun yang diperoleh dari daerah Bais memiliki ukuran terkecil sedangkan lamun yang berasal dari Banilad yang tertinggi; 2) Diantara semua spesies yang teramati, Cymodocea rotundata tidak menunjukkan variasi morfometrik Kata Kunci: morfometrik, akar, daun terpanjang   ANALISIS MORFOMETRIK SPESIES LAMUN DI NEGROS ORIENTAL ABSTRACT A study of morphometric variations of five seagrass species in Negros Oriental, the Philippines was conducted from January to March 20011. Measurements of the following morphological variables were taken: length of rhizomes, length of upright shoot, length of longest leaf (cm). The study showed that: 1) Seagrass species from Bais had the lowest size range in morphometric measurements, whereas those from Banilad had the highest; 2) Among all the species observed, Cymodocea serrulata did not show morphometric variations. Keywords: morphometric, rhizome, longest leaf
DNA Isolation And Amplification of the rbcL (ribulose-1,5- bisphosphate carboxylase/oxygenase large subunit) gene of Caulerpa sp., Gracilaria sp., And Sargassum sp. Biondi Tampanguma; Grevo S. Gerung; Veibe Warouw; Billy Th Wagey; Stenly Wulllur; Deiske A. Sumilat; Hens Onibala
Jurnal Ilmiah PLATAX Vol. 8 No. 2 (2020): ISSUE JULY-DECEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.8.2.2020.30003

Abstract

DNA isolation and gene amplification of algae are significantly influenced by various factors such as characteristics and components of the algae cell wall. Therefore techniques and methods of DNA isolation in certain algae, sometimes only succeed in one particular species and can not be applied to another algae species. Based on that issue, this study was conducted with the aims to determine the succeed of DNA isolation and amplify the rbcL gene as a target gene for identification. Algae DNA was isolated by using innuPrep Plant DNA commercial kit, and the second one with a modified conventional Cetyl Trimetyl Ammonium Bromide (CTAB) method,  for the amplification process was using rbcL gene (ribulose-1,5-bisphosphate carboxylase / oxygenase large subunit) with two pairs of primers : rbcL 7F-753R and rbcL 577F-rbcSR. The results showed that the DNA of Gracilaria sp was succeed isolated by using CTAB method and it was denoted by the presence of DNA bands in agarose gel. Meanwhile DNA amplification for Gracilaria sp., and Sargassum sp., were succeed amplified with the appearance of DNA bands. But in algae Caulerpa sp., was only succeed on 1 pair of primary rbcL 7F and 7.Keywords : DNA, gene rbcL, algae Caulerpa sp., Sargassum sp., Gracilaria sp;AbstrakIsolasi DNA dan amplifikasi gen pada alga sangat dipengaruhi oleh beberapa faktor seperti karakter dan komponen pada dinding sel alga. Oleh karena itu proses isolasi DNA terkadang bisa berhasil pada satu jenis alga, namun tidak berhasil pada jenis alga lainnya. Oleh karena alasan tersebut, maka penelitian ini dilakukan untuk menentukan keberhasilan Isolasi DNA dan mengamplifikasi gen rbcL sebagai gen target identifikasi. Penelitian ini dilakukan dengan tahapan awal Isolasi DNA yang menggunakan kit komersil innuPrep Plant DNA Kit, dan metode konvensional Cetyl Trimetyl Ammonium Bromide (CTAB) yang telah dimodifikasi. Sedangkan untuk proses amplifikasi, menggunakan gen rbcL (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit) digunakan dua pasang primer yaitu rbcL 7F-753R dan rbcL 577F-rbcSR. Hasil isolasi DNA dari alga Gracilaria sp berhasil diisolasi menggunakan metode CTAB yang ditandai dengan adanya pita DNA pada gel agarose. Amplifikasi DNA pada alga Gracilaria sp., dan Sargassum sp., berhasil diamplifikasi dengan munculnya pita DNA. Namun pada alga Caulerpa sp. hanya berhasil pada 1 pasang primer rbcL 7F dan753R.Kata kunci : DNA, gen rbcL, alga Caulerpa sp., Sargassum sp., Gracilaria sp.
STUDI PERUMUSAN STRATEGI PENGELOLAAN EKOWISATA BAHARI KOTA MANADO DI ERA REVOLUSI INDUSTRI 4.0 BERDASARKAN ANALISIS SWOT Sarif Hidayat; Antonius P Rumengan; Suria Darwisito; Medy Ompi; Winda M Mingkid; Billy Th Wagey; Carolus P Paruntu
JURNAL PESISIR DAN LAUT TROPIS Vol. 7 No. 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24421

Abstract

The objective of this research was to formulate a strategy for managing maritime ecotourism in the era of industrial revolution 4.0 in the city of Manado. The study was conducted at the coastal areas of the mainland of Manado City and Bunaken Island for 3 months, February - May 2019. The research method was a survey with descriptive analysis and SWOT. The results of the study obtained 4 strategy formulations (key success factors) in the context of managing maritime ecotourism in the industrial revolution era 4.0 in the city of Manado, namely: 1) Increasing law enforcement in the field of marine ecotourism, waste management on land and sea, 2) Awareness of the community environmental hygiene both on land and sea, 3) Empowering biodiversity resources of coral reefs, seagrass beds and mangroves in the park for developing marine maritime ecotourism through digital applications, and 4) managing and developing resorts, coastal culinary attractions, points of diving spots, and tourism  ports. The results of the SWOT curve show the condition of marine ecotourism in the city of Manado in quadrant 2, namely a situation where the threat to the development of marine ecotourism is more dominant than opportunity, but there are strengths of tourism organizations that can be relied on. Stakeholders are expected to be able to improve performance so that quadrant 2 conditions change to quadrant 1, which is to support an aggressive strategy: a very good situation because of the power that is used to seize profitable opportunities. In the era of industrial revolution 4.0, every stakeholder in the maritime ecotourism industry in the city of Manado was supposed to change the management system towards digital-based by making applicationsKeywords: Bunaken island, revolution industry 4.0, Manado city, marine ecotourism, strategyTujuan penelitian adalah untuk merumuskan strategi pengelolaan ekowisata bahari di era revolusi industri 4.0 di Kota Manado.  Penelitian dilakukan di wilayah pesisir daratan Kota Manado dan Pulau Bunaken selama 3 bulan, Februari - Mei 2019. Metode penelitian adalah survei dengan analisis deskriptif dan SWOT. Hasil penelitian diperoleh 4 rumusan strategi (faktor-faktor kunci keberhasilan) dalam rangka pengelolaan ekowisata bahari era revolusi industri 4.0 di Kota Manado, yaitu: 1) Meningkatkan penegakan hukum di bidang ekowisata bahari, pengelolaan sampah di darat maupun laut, 2) Menyadarkan masyarakat tentang kebersihan lingkungan baik di daratan maupun lautan, 3) Memberdayakan sumber daya keanekaragaman hayati terumbu karang, padang lamun dan mangrove di kawasan TNB untuk pengembangan ekowisata bahari melalui aplikasi digital, dan 4) Mengelola dan mengembangkan resort, tempat-tempat wisata kuliner pantai, titik-titik penyelaman, dan pelabuhan pariwisata.  Hasil kurva SWOT memperlihatkan kondisi ekowisata bahari Kota Manado berada dalam kuadran 2, yaitu situasi dimana ancaman terhadap pengembangan ekowisata bahari lebih dominan dibandingkan peluang, namun ada kekuatan organisasi kepariwisataan yang dapat diandalkan. Pemangku kepentingan diharapkan dapat meningkatkan kinerja agar kondisi kuadran 2 berubah menjadi kuadran 1, yaitu mendukung strategi agresif: situasi yang sangat baik karena adanya kekuatan yang dimanfaatkan untuk meraih peluang yang menguntungkan.  Dalam era revolusi industri 4.0, maka setiap pemangku kepentingan industri ekowisata bahari di Kota Manado sudah seharusnya merubah sistem pengelolaan yang ada ke arah berbasis digital dengan cara membuat aplikasi pengelolaan ekowisata bahari.Kata Kunci: Ekowisata bahari, revolusi industri 4.0, Kota Manado, Pulau Bunaken, strategi
FORAMINIFERA BENTIK PADA PADANG LAMUN DI KAWASAN PANTAI SEKITAR PULAU BUNAKEN SULAWESI UTARA Feby GB Kombo; Jane M Mamuaja; Royke M Rampengan; Billy Th Wagey; Calvyn FA Sondakh; Henneke Pangkey
JURNAL PESISIR DAN LAUT TROPIS Vol. 7 No. 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24468

Abstract

Benthic foraminifera lives on the seaflor and some of them are attached to plant stem. This organisms use pseudopodia to move. The study aims to describe the groups of foraminifera that live on leaves and seagrass sediments and provide information on the comparison of the number of benthic foraminifera genera in seagrass beds. The study was performed in Bunaken Island and during the study a number of 4593 specimens of benthic foraminifera has been identified and they are divided into specimen that lives on seagrass leaves (1097 specimens) and specimens that live on sediments (3496 specimens). The specimen was grouped in 16 genera and they were scattered in 2 stations within four sampling points. These genera are: Amphistegina, Calcarina, Coscinospira, Elphidium, Eponides, Lachlanela, Marginophora, Neorotalia, Operculina, Cleroplis, Planorbulina, Pseudorotalia, Quinqueloculina, Sorites, Spiroluculina, and Triloculina. Benthic foraminifera in seagrass leaves consist of 16 genera which are characterized by the genus Marginophora and Amphistegina and those live in substratum consist of 14 genera which was characterized by the genus Amphistegina.Keywords : Benthic Foraminifera, Seagrass beds, Bunaken Island
DIMENSI DAN KOMPOSISI NEMATOSIT PADA KARANG SCLERACTINIA, Acropora florida DAN Acropora divaricata DI PANTAI MOTANDOI SELATAN KABUPATEN BOLAANG MONGONDOW SELATAN Sanjay Gagu; Suria Darwasito; Billy Th Wagey; Antonius P Rumengan; Ari B Rondonuwu; Carolus P Paruntu
JURNAL PESISIR DAN LAUT TROPIS Vol. 7 No. 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24499

Abstract

The purpose of this study was to determine the type, dimensions and composition of nematocytes in Scleractinia, Acropora florida and Acropora divaricata.  This research was conducted in South Motandoi Beach, East Pinolosian District, South Bolaang Mongondow Regency, North Sulawesi for five (5) months; from February to June 2019.  The method of this research was observation using student-t statistical data analysis. Microbasic p-Mastigophore type-II (MpM-II) and Holotricous isorhiza (HI), while A. divaricata only has type-II Microbasic p-Mastigophore (MpM-II) nematocytes.  The nematocytes dimension showed an average capsule length of MpM II in A. florida of was 262.32 µm and A. divaricata was 125.15 µm, mean capsule width of MPM II in A. florida was 67.01 µm and A. divaricata was 31.95 µm, and the average length of mpM II on A. florida was 154.44 µm, and A. divaricata was 70.97 µm.  Nematocytes composition showed that MPM II in A. florida was 95.56% and HI was 4.44%, while the composition of MPM II nematocytes in A. divaricata was 100% and HI was 0%.  Student t-test showed that the ratio of 2 average values of capsule length, capsule width, and MPM II stem length from two populations of A. florida and A. divaricata were not significantly different (P> 0.05).   It can be concluded that both types of A. florida and A. divaricata corals have different types and composition of nematocytes however their size dimensions are not significantly different.  Further studies are important to examine genetic and environmental factors to find out how much the kinship relationship between the two types of those coral.Keywords: Acropora florida, Acropora divaricata, HI, Scleractinia coral, MpM II, nematocytes
IKAN YANG BERUAYA DI DAERAH MANGROVE PANTAI TASIK RIA Otinus Lokbere; Farnis B Boneka; Chatrien A Sinyal; Billy Th Wagey; Medy Ompi; Rose OSE Mantiri
JURNAL PESISIR DAN LAUT TROPIS Vol. 7 No. 3 (2019): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.7.3.2019.24515

Abstract

This study is intended to find out the fish that are cultured into the mangrove area of Tasik Ria beach, Mokupa, Minahasa Regency. Fish is obtained through gill jarring at high tide. The fish that were identified were Archamia fucata, Lutjanus fulvus, Lutjanus eherenbergii, Lethrinus ornatus, Scolopsis lineate, Sargocentron diadema, Epinephelus merra and Scorpaenopsis oxycephala. The fish are generally carnivorous, and are nocturnal.Key words: Tasik Ria Beach, the fishes, carnivorous, mangroves
Co-Authors Antonius P Rumengan Antonius P Rumengan Ari B Rondonuwu Biondi Tampanguma Calvyn F. A. Sondak, Calvyn F. A. Carolus P Paruntu Chatrien A Sinyal Deiske A. Sumilat Farnis B Boneka Feby GB Kombo Grevo S. Gerung Henneke Pangkey Hens Onibala Jane M Mamuaja Medy Ompi Otinus Lokbere Rose OSE Mantiri Royke M Rampengan Sanjay Gagu Sarif Hidayat Stenly Wulllur Suria Darwasito Suria Darwisito Veibe Warouw Winda M Mingkid