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All Journal BIOEDUSCIENCE Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Biofarm : Jurnal Ilmiah Pertanian Jurnal HPT (Hama Penyakit Tumbuhan)
Hery Nirwanto, Hery
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering

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Journal : BIOEDUSCIENCE

 1 
Efikasi Formulasi Granular Kombinasi Trichoderma sp. dan Ekstrak Daun Bandotan terhadap Sclerotium rolfsii pada Porang (Amorphophallus oncophyllus) Raniah, Nabilla Nur; Nirwanto, Hery; Suryaminarsih, Penta
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14880

Abstract

Background: Amorphophallus oncophyllus is a tuber plant that is beneficial for various industrial sectors, but in its cultivation, it is often affected by the pathogen Sclerotium rolfsii, requiring control measures. Control efforts can be carried out using a combination of the biological agent Trichoderma sp. and the plant pesticide made from bandotan leaf extract. The combined control application can be formulated in a solid granular form. Method: This research was conducted both in vitro and in vivo. In vitro, a Completely Randomized Design (RAL) was used with the combination of Trichoderma sp. and bandotan leaf extract, divided into four levels: 0% (TB0), 5% (TB5), 10% (TB10), and 15% (TB15). In vivo, a 2-factor RAL was applied. The first factor was the concentration of the rice flour carrier material in four levels: 0% (B1), 25% (B2), 50% (B3), and 75% (B4). The second factor was the storage duration of the granular formulation, consisting of 0 weeks (M1), 3 weeks (M2), and 5 weeks (M3). Result: The study's results showed that the granular combination of Trichoderma sp. and 10% bandotan leaf extract reduced S. rolfsii infection by 64.25% and stimulated the growth of porang plant height. Conclusion: The treatment with the granular combination of Trichoderma sp. and 10% bandotan leaf extract, adding 25% rice flour and a storage period of 0-3 weeks, showed the best effect on the porang plant seedlings.
Pengaruh Konsentrasi Kalsium Klorida pada Viabilitas dan Daya Sweelling Beads Enkapsulasi Paenibacillus polymyxa secara In Vitro Sari, Zulfa Novita; Nirwanto, Hery; Lestari, Safira Rizka
BIOEDUSCIENCE Vol 9 No 2 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/16364

Abstract

Background: Paenibacillus polymyxa is a potent antagonist that can be utilized as a biological agent. The use of biological agents has gained interest among farmers, but their application in liquid formulations has not consistently maintained the optimal stability and viability of microorganisms. One promising approach to overcome this problem is bioencapsulation, which is a method of wrapping biological agents to protect bacteria in the soil and increase their efficiency. This study aims to assess the encapsulation efficiency, measure the viability of microorganisms in the beads, and determine the difference in swelling power of beads made with various concentrations of calcium chloride (CaCl2) as a binding agent. Method: Beads were produced using the extrusion method by combining Paenibacillus polymyxa suspension, sodium alginate suspension, and adding CaCl2 at concentrations of 1%, 3%, and 5%. Results: The results showed that different CaCl2 concentrations can affect the viability of Paenibacillus polymyxa in beads. Beads made with CaCl2 at 3% concentration were the best results in the encapsulation efficiency test compared to beads made with 1% and 5% CaCl2 binders. In comparison, beads with 3% and 5% CaCl2 concentrations were able to maintain the viability of microorganisms at a higher level and for a longer time than beads using CaCl2 at 1% concentration. The decrease in viability and swelling power of the beads is thought to be caused by the carrier material used and the storage conditions. Conclusion: Bead treatment with 3% calcium chloride concentration was the best treatment for encapsulation efficiency in absorbing Paenibacillus polymyxa, amounting to 98.21%.
The Effect of Packaging and Encapsulation Temperature of Red Chili Seeds with Trichoderma sp. on Viability and Inhibition of Fusarium sp. Ratu, Dewanggie Sasmita; Nirwanto, Hery; Mujoko, Tri
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18020

Abstract

Background: Fusarium sp. is one of the main threats to chili cultivation, thereby reducing its economic value. Therefore, the use of biological microorganisms, such as Trichoderma sp., is an environmentally friendly alternative for controlling Fusarium sp. Application techniques for Trichoderma sp. biological agents include seed coating or encapsulation. However, ensuring the quality and effectiveness of encapsulated products during storage and distribution remains a concern. Thus, this study aims to examine the effects of packaging type and storage temperature on the viability and efficacy of Trichoderma sp. biological agents encapsulated in red chili seeds (Capsicum annuum L.) for inhibiting Fusarium sp. Methods: This study applied a completely randomized design (CRD) factorial with two factors, namely packaging material (aluminum foil and plastic) and storage temperature (5°C, 28°C, and 36°C), resulting in 7 treatments with three replications plus a control, resulting in a total of 21 experimental units. Results: Aluminum foil packaging stored at 28°C showed the highest viability of Trichoderma sp. and the most effective antagonistic activity against Fusarium sp. Conclusion: These results provide a sustainable, environmentally friendly solution for biological-agent-based seed storage.
Co-Authors Hasbulloh, Mu'arif Ihza Izza, Salsa Bila Ari Nur Lestari, Safira Rizka Noni Rahmadhini Penta SURYAMINARSIH Putra, Dandi Rahmansyah Ramadhini, Noni Raniah, Nabilla Nur Ratu, Dewanggie Sasmita Sari, Zulfa Novita Sri Wiyatiningsih Syamsiar, Syamsiar Tri MUJOKO Triwahyu, Endang