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All Journal BIOEDUSCIENCE Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Biofarm : Jurnal Ilmiah Pertanian
Hery Nirwanto, Hery
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering

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Efikasi Formulasi Granular Kombinasi Trichoderma sp. dan Ekstrak Daun Bandotan terhadap Sclerotium rolfsii pada Porang (Amorphophallus oncophyllus) Raniah, Nabilla Nur; Nirwanto, Hery; Suryaminarsih, Penta
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14880

Abstract

Background: Amorphophallus oncophyllus is a tuber plant that is beneficial for various industrial sectors, but in its cultivation, it is often affected by the pathogen Sclerotium rolfsii, requiring control measures. Control efforts can be carried out using a combination of the biological agent Trichoderma sp. and the plant pesticide made from bandotan leaf extract. The combined control application can be formulated in a solid granular form. Method: This research was conducted both in vitro and in vivo. In vitro, a Completely Randomized Design (RAL) was used with the combination of Trichoderma sp. and bandotan leaf extract, divided into four levels: 0% (TB0), 5% (TB5), 10% (TB10), and 15% (TB15). In vivo, a 2-factor RAL was applied. The first factor was the concentration of the rice flour carrier material in four levels: 0% (B1), 25% (B2), 50% (B3), and 75% (B4). The second factor was the storage duration of the granular formulation, consisting of 0 weeks (M1), 3 weeks (M2), and 5 weeks (M3). Result: The study's results showed that the granular combination of Trichoderma sp. and 10% bandotan leaf extract reduced S. rolfsii infection by 64.25% and stimulated the growth of porang plant height. Conclusion: The treatment with the granular combination of Trichoderma sp. and 10% bandotan leaf extract, adding 25% rice flour and a storage period of 0-3 weeks, showed the best effect on the porang plant seedlings.
Pengaruh Konsentrasi Kalsium Klorida pada Viabilitas dan Daya Sweelling Beads Enkapsulasi Paenibacillus polymyxa secara In Vitro Sari, Zulfa Novita; Nirwanto, Hery; Lestari, Safira Rizka
BIOEDUSCIENCE Vol 9 No 2 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/16364

Abstract

Background: Paenibacillus polymyxa is a potent antagonist that can be utilized as a biological agent. The use of biological agents has gained interest among farmers, but their application in liquid formulations has not consistently maintained the optimal stability and viability of microorganisms. One promising approach to overcome this problem is bioencapsulation, which is a method of wrapping biological agents to protect bacteria in the soil and increase their efficiency. This study aims to assess the encapsulation efficiency, measure the viability of microorganisms in the beads, and determine the difference in swelling power of beads made with various concentrations of calcium chloride (CaCl2) as a binding agent. Method: Beads were produced using the extrusion method by combining Paenibacillus polymyxa suspension, sodium alginate suspension, and adding CaCl2 at concentrations of 1%, 3%, and 5%. Results: The results showed that different CaCl2 concentrations can affect the viability of Paenibacillus polymyxa in beads. Beads made with CaCl2 at 3% concentration were the best results in the encapsulation efficiency test compared to beads made with 1% and 5% CaCl2 binders. In comparison, beads with 3% and 5% CaCl2 concentrations were able to maintain the viability of microorganisms at a higher level and for a longer time than beads using CaCl2 at 1% concentration. The decrease in viability and swelling power of the beads is thought to be caused by the carrier material used and the storage conditions. Conclusion: Bead treatment with 3% calcium chloride concentration was the best treatment for encapsulation efficiency in absorbing Paenibacillus polymyxa, amounting to 98.21%.
EKSPLORASI BAKTERI Bacillus spp. PADA PERAKARAN TANAMAN KENTANG (Solanum tuberosum L.) SEBAGAI AGENSIA PENGENDALI HAYATI PATOGEN Fusarium sp. ASAL LAHAN WONOKITRI KABUPATEN PASURUAN JAWA TIMUR Paisal, Paisal; Triwahyu, Endang; Nirwanto, Hery
Agros Journal of Agriculture Science Vol 25, No 4 (2023): edisi Oktober
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i4.3548

Abstract

The potato plant (Solanum tuberosum L.) is a horticultural commodity that can be used as a source of carbohydrates and has the potential to fulfill food needs. Plant pest organisms that are often found in potato plants are soil-borne pathogenic fungi with an attack intensity of 50% so they can cause crop yield losses of up to 100%. Controlling soil-borne pathogens of potato plants can use the biological agent Bacillus sp. One of the ingredients is an extracellular enzyme based on the abilities of bacillus sp. So this research aims to determine the effect of bacillus sp. can inhibit the growth of fusarium wilt disease. Bacillus sp. The bacteria used in this research were exploration bacteria from Wonokitri land, Pasuruan Regency, East Java, namely isolate I17. The method for carrying out this research is taking potato plant soil samples, isolation, rejuvenation of Bacillus sp. bacteria, 3% KOH test, catalase test, pathogenicity test, gram bacillus staining, antagonist test. Observation of the morphology of Fusarium sp. post in vitro antagonist test. Isolate I17 produced fusarium hyphae morphology that experienced abnormal development, namely bending, shrinking, lysis, twisting and curling. The research was carried out from December 2022 to April 2023 in the Health Laboratory, Department of Agrotechnology, Faculty of Agriculture, Veterans National Development University, East Java. Key words: Potato Plants, Fusarium sp, Bacillus sp INTISARITanaman kentang (Solanum tuberosum L.) merupakan salah satu komoditas hortikultura yang dapat dipakai sebagai sumber karbohidrat dan berpotensi untuk pemenuhan kebutuhan pangan. Organisme pengganggu tanaman yang sering ditemukan pada tanaman kentang adalah jamur patogen tular tanah dengan intensitas serangan 50% sehingga dapat menimbulkan kerugian hasil panen hingga 100%. Pengendalian patogen tular tanah tanaman kentang dapat menggunakan agensia hayati bacillus sp. Yang salah satu kandungannya adalah enzim ektraseluler berdasarkan kemampuan yang dimiliki bacillus sp. Maka penelitian ini bertujuan untuk mengetahui pengaruh bacillus sp. dapat menghambat pertumbuhan penyakit layu fusarium. Bakteri bacillus sp. yang digunakan dalam penelitian ini merupakan bakteri eksplorasi dari lahan wonokitri Kabupaten Pasuruan Jawa Timur yaitu isolat I17. Metode pelaksanaan penelitian ini yaitu pengambilan sampel tanah tanaman kentang, isolasi, peremajaan bakteri Bacillus sp., uji KOH 3%, uji katalase, uji patogenisitas, pewarnaan gram bacillus, uji antagonis. Pengamatan morfologi Fusarium sp. pasca uji antagonis in vitro. Isolat I17 menghasilkan morfologi hifa fusarium mengalami perkembangan yang abnormal, yaitu membengkok, mengecil, lisis, melilit dan keriting. Penelitian dilaksanakan dari bulan Desember 2022 sampai bulan April 2023 di laboratorium Kesehatan, Jurusan Agroteknologi, Fakultas Pertanian, Universitas Pembangunan Nasional Veteran Jawa Timur.Kata kunci: Tanaman Kentang, Fusarium sp,  Bacillus sp
Geostatistical Approach and Drone Image Analysis of the Spatial Distribution of Bacterial Leaf Blight in Rice Plants Nirwanto, Hery; Wiyatiningsih, Sri; Izza, Salsa Bila Ari Nur
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 2 (2025): April 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i2.582-593

Abstract

Bacterial leaf blight caused by the bacterium Xanthomonas oryzae is an important disease because it can cause severe damage and can infect the vegetative and generative phases of rice plants. This research was carried out to determine the spatial distribution of leaf blight using a geospatial approach and drone imagery. The results of this research indicate that the distribution of bacterial leaf blight disease on land at the research location tends to be higher in the northern part compared to the southern part of the land. The attack rate value at the end of the observation ranged between 20-68%. The distribution of leaf blight on land can be described through simulations using a geostatistical approach and confirmed by aerial imagery. Aerial imagery, especially binary imagery and kriging imagery, mutually confirm (crosscheck) the occurrence of leaf blight on land. Aerial images that are processed into binary images have the potential to be a remote sensing method that can make it easier to observe the distribution of diseases on land, especially leaf blight. Keywords: Campestris, Drone Imagery, Geospatial, Rice, Xanthomona.
Pemetaan Serangan Hama Penggerek Pucuk Tebu dengan Geostatistika Putra, Dandi Rahmansyah; Nirwanto, Hery; Ramadhini, Noni
Biofarm : Jurnal Ilmiah Pertanian Vol. 20 No. 1 (2024): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v20i1.4509

Abstract

Produktivitas tanaman tebu (Saccharum officinarum) dipengaruhi oleh berbagai faktor salah satunya adalah serangan hama penggerek pucuk yang disebabkan oleh (Scirpophaga excerptalis). Oleh sebab itu, perlu dilakukan upaya untuk menekan kerusakan tanaman melalui Pengendalian Hama Tanaman Terpadu sehingga membutuhkan informasi monitoring yang tepat. Pemetaan menggunakan Geostatistika pada beberapa tahun terakhir mendukung peningkatan akurasi dan efisiensi monitoring melalui aerial monitoring pada bidang pertanian. Penelitian ini bertujuan untuk mengkaji pemetaan Geostatistika dalam mendeteksi gejala beserta pola serangan hama penggerep pucuk pada tanaman tebu melalui analisis ground monitoring lalu dipetakan, Penelitian ini dilaksanakan di kebun pertanaman tebu rakyat di Kecamatan Sukodono, Kabupaten Sidoarjo, Provinsi Jawa Timur, Indonesia pada bulan Februari hingga Maret 2023. Penelitian ini dilaksanakan menggunakan metode survey sesuai sasaran hama dengan menggunakan dua jenis pengamatan yaitu pengamatan secara konvensional (ground monitoring). Berdasarkan analisis data dengan membandingkan pemetaan dilapang dengan hasil pemetaan digital yang diolah dengan pendekatan geostatistik, didapatkan bahwa pemetaan secara geostatistik berpotensi untuk mendeteksi gejala dan pola sebaran hama penggerek pucuk. Hasil pengamatan selama 6 minggu menunjukkan pola sebaran terjadi secara mengelompok dibagian barat laut serta bagian timur.Kata kunci: geostatistika, kriging, penggerek pucuk,tebu,variogram
Co-Authors Izza, Salsa Bila Ari Nur Lestari, Safira Rizka PAISAL PAISAL Penta SURYAMINARSIH Putra, Dandi Rahmansyah Ramadhini, Noni Raniah, Nabilla Nur Sari, Zulfa Novita Sri Wiyatiningsih Triwahyu, Endang