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All Journal Jurnal Ilmiah Farmasi Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Majalah Farmaseutik Indonesian Journal of Chemistry
Purwantiningsih Purwantiningsih
Department Of Pharmacology And Clinical Pharmacy, Faculty Of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Medicine & Pharmacology Public Health

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EFFECT OF THE JUICE OF GINGER RHIZOMES ON THE PHARMACOKINETICS OF SULFAMEZATHINE IN RATS ., Purwantiningsih; Hakim, Lukman
INDONESIAN JOURNAL OF PHARMACY Vol 14 No 1, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.267 KB) | DOI: 10.14499/indonesianjpharm0iss0pp250-255

Abstract

The rhizome of ginger (Zingiber officinale, Rosc.) has been widely consumed by people as food ingredient or a traditional drug. In the previous research there was evidence that the ginger juice influenced the pharmacokinetics of propranolol, a representative of drugs with high extraction ratio. The aim of this research is to study the effect of ginger juice on another high ER drug, sulfamezathine. To achieve the aim of this study, rats were divided into two groups to get the blood and urine data. The animals at the blood data group were divided into two groups. The group I (N=6) was given sulfamezathine (50 mg/kg BW) through intra peritoneal injection and the group II (N=6) was pretreated with the ginger juice orally (4 ml/kg BW) an hour prior to administration of sulfamezathine. The blood samples were withdrawn at various intervals after the drug administration. The unchanged sulfamezathine concentration was determined using a modified Bratton-Marshall method. From this blood data the values of Ka, Cmaks, Tmaks, Vdss, AUC, Cl and T1/2el were computed. Urine data was obtained by using two groups of rats with the same treatment as above. The sulfamezathine was counted from urine which accumulated for 24 hours to obtain the value of Fel. The results of this research showed that significant decreases in Cmaks (16.72%) and AUC (22.92%) values have occurred, with increasing values of Vdss (18.39%), ClT (38.29%), ClR (213.11%) and Fel 24 hours (128.58%) values. Key words : ginger rhizome, pharmacokinetics, sulfamezathine, rats
EFEK ANALGESIK FRAKSI ETANOL DARI EKSTRAK ETANOL DAUN MINDI (Melia azedarach L.) PADA MENCIT JANTAN Indah Purwantini,; Purwantiningsih Purwantiningsih; Oktavia Eka Puspita
Jurnal Ilmiah Farmasi Vol. 4 No. 2 (2007)
Publisher : Universitas Islam Indonesia

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Abstract

ABSTRACTIndonesia has a lot of traditional medicines, which people used long time ago, one of theseis mindi (Melia azedarach L.). It have been known that ethanolic extract of mindi leaves hasanalgesic effect greater than paracetamol at dose 6.44 mg/kgBW and 12.89 mg/kgBW. Thisresearch conducted to find out the effectiveness ethanolic fraction of ethanolic extract as ananalgesic. The research was carried out in completely random one way design, used 35 micewhich have been fasted for 24 hours. The mice were divided into 7 groups i.e. negative control,positive control and 5 testing groups (in different doses). Fifteen minutes after injected with thefractions of mindi, the mice were given acetic acid 0.5% intraperitoneally injection. The cumulativewrithe reflects were calculated in every 5 minutes for one hour and were counted the protectionpercentage. Results showed that the ethanol fractions doses 12,88-103,04 mg/kgBW of mindileaves have the analgesic effect in mice and gave protection percentage 30.93-71.61%. Thechromatograms of TLC indicated that the fraction contain flavonoids and phenolic compounds.Key words: ethanol fraction of mindi, analgesic effect, TLC profile
Antioxidant Activities of n-Hexane Soluble and Insoluble Fraction, Ethyl Acetate Soluble and Insoluble Fraction from Ethanol Extract of Sambung Nyawa Leaf (Gynura procumbens (Lour.) Merr.) Purwantiningsih Purwantiningsih; Retno Murwanti; Lukman Hakim
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.43743

Abstract

There were many plants containing flavonoids which can function as antioxidant. Antioxidant can be used to decrease the mortality rate caused by degenerative and infectious diseases. This study aims to determine the antioxidant potency of n-hexane soluble and insoluble fraction, ethyl acetate soluble and insoluble fraction, and the ethanol extract antioxidant activity of Sambung Nyawa leaf (Gynura procumbens (Lour.) Merr.). The ethanol extract of G. procumbens leaves was obtained by maceration using 70% ethanol then fractionated using hexane and ethyl acetate to obtain the n-hexane soluble fraction, n-hexane insoluble fraction, ethyl acetate soluble fraction, and ethyl acetate insoluble fraction. The antioxidant activity study of ethanol extract, n-hexane soluble, and insoluble fraction, ethyl acetate soluble, and insoluble fraction of G. procumbens was carried out using DPPH (2,2- diphenyl-1-picrylhydrazyl) method to evaluate its free radical scavenging activity with a comparison of vitamin C. The antioxidants activity was expressed as IC50 value. The IC50 value for vitamin C and ethanol extract of G. procumbens leaves were 2.41 µg/ml and 200 µg/ml. On the other hand, the n-hexane soluble and insoluble fraction had an IC50 value of 78 µg/ml and 34 µg/ml, while ethyl acetate soluble and insoluble fraction had the potency as an antioxidant with an IC50 value of 419 µg/ml and 151 μg/ml, respectively. Based on the results obtained, the antioxidant potency of G. procumbens ethanol extract and their fraction were successively of n-hexane insoluble fraction > n-hexane soluble fraction > ethyl acetate insoluble fraction > ethanol extract > ethyl acetate soluble fraction.
Antimicrobial activity and Identification of fungus associated Stylissa flabelliformis sponge collected from Menjangan Island West Bali National Park, Indonesia Erna Prawita Setyowati; Silvia Utami Tunjung Pratiwi; Purwantiningsih Purwantiningsih; Oka Samara
Indonesian Journal of Pharmacy Vol 29 No 2, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1684.892 KB) | DOI: 10.14499/indonesianjpharm29iss2pp66

Abstract

The Fungus is a very important microorganism as a producer of bioactive secondary metabolites. Active substances of microbial origin have been sought through the process of screening methods to obtain antimicrobial compounds. The purpose of this study was to isolate fungi associated with sponge taken from Menjangan Island National Park West Bali (Indonesia) and identify fungi that have antimicrobial activity. Isolation of fungus from sponge was carried out by spread plate method using Saboroud Saline Agar medium. Each fungi will be tested to Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Identification of fungi is based on the observation of macroscopic, microscopic and also using 16rRNA/ITS phylogeny tree. The results showed that S. flabelliformis sponge had 10 fungal isolates. Most of them have antimicrobial activity. The name associated with a sponge fungus is These 10 fungus are Aspergillus flavus strain UPMZ02, Aspergillus fumigatus strain CD1621, Trichoderma reesei strain JCM 2267, Aspergillus nomius strain KUB105, Aspergillus sp. strain TLWK-09, Aspergillus flavus strain MC-10-L, Penicillium sp. strain RMA-2, Aspergillus sp. strain TLWK-09, Aspergillus fumigatus and Trichoderma reesei strain TV221 
Major Metabolites of Methylripariochromene-A, a Bioactive Substance in The Leaves of Eupatorium Riparium Reg. in Male Rats Urine Subagus Wahyuono; Charles S. Vairappan; Datin Maryati Mohd.; Vera Fauziati; Purwantiningsih Purwantiningsih; Marchaban Marchaban
Majalah Farmaseutik Vol 14, No 1 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.007 KB) | DOI: 10.22146/farmaseutik.v14i1.41928

Abstract

Methylripariochromene-A (MRC) (1) is the principal bioactive compound isolated from Eupatorium riparium Reg. collected from the Mt. Merapi National Park. MRC was isolated from the chloroform extract of the leaves by using bioassay (BST) guided isolation method. MRC causes diuretic, reduces the blood pressure and excretes mineral from the body. This activity is comparable to a clinically used diuretic drug, furosemide. MRC is a chromene type of compound, having 2 –OCH3 groups on the aromatic ring that are possibly demethylated in vivo to give phenolic compounds well known for having antiseptic properties. This study was aimed to evaluate diuretic properties compared to furosemide, isolate and identify MRC major metabolites present in urine. If it is true, then MRC probably can be used also as anti-infectious diseases in the tractus urinary. n-Hexane extract (81% MRC) of E. riparium (1.25 g/kg bw) was applied p.o on male rats (+ 200g) and furosemide was used as a positive control of diuretic activity. Urine was collected during 48 hours, partitioned with EtOAc for 15 minutes to give EtOAc fraction at the upper layer. The EtOAc fraction was then checked by tlc with MRC as the reference standard. The major metabolites were isolated by fractionating the EtOAc fraction followed by purification using preparative tlc (fr. 4-5 and 6-7). The major metabolites obtained were identified based on their 1H- and 13C-NMR data. Diuretic effect of n-hexane extract (1.25 g/kg bw) was comparable to furosemide (8.83 mL/rat vs 8.70 mL/rat for 24 hours). The 1H- and 13C-NMR data of fr 4-5 indicated that there was not major changing in the 1H- and 13C-NMR spectra appearance compared to that of MRC, except that 1 –OCH3 group was demethylated became demethyl MRC (2) . Based on HMBC and HMQC experiments, -OCH3 at C-8 was the one demethylated. The 1H-NMR data of fr. 6-7 was similar to fr. 4-5, demethylation of one –OCH3 group on C-8 but the ether linkage in the furan ring system was broken to give 2 tentative structures (3 and 4).
Ex-Vivo Study, The Effect of Standardized Eurycoma longifolia Extract on The Enzyme Activity of Rosiglitazone N-Demethylase Purwantiningsih Purwantiningsih; Abas Hj Hussin; Kit Lam Chan
Majalah Farmaseutik Vol 17, No 1 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v17i1.48481

Abstract

Eurycoma longifolia (E. longifolia) or also known as Earth Pasak in Indonesia, has been used widely, especially to increase stamina in men. The use of herbal medicines in the long run opens up the opportunities for the influence of herbal medicines on metabolic process of other substances. This study aimed to evaluate the effect of E. longifolia extract on rosiglitazone metabolism after oral administration for one and fourteen days, and its effect on rat body weight. The rats were divided into two groups, group for one day treatment (divided into 8 subgroups with n = 6: I (control), II to VIII (were given extract at doses of 1 to 1000 mg / kg BW) and 14 days (rats divided into 4 subgroups, n = 6 : I (control), II to IV (treated with extracts of doses 5, 25 and 50 mg / kg BW). At the end of the experiment, test animals were sacrificed and rosiglitazone N-demethylase activity in hepatocytes was determined by measuring the amount of formaldehid formed at 415 nm. The rosiglitazone N-demethylase activity in all groups was analyzed by analysis of  variance (ANOVA) and Tukey Test (P <0.05). The percentage of the changing in the body weight in the 14 days treatment group was compared to the control group. The results showed there was a significant increased in the rosiglitazone N-demethylase activity after rats were treated with E. longifolia extract at doses of 5 to 1000 mg / kg BB, but was not significantly different at a dose of 1 mg / kg BW when compared with control group. For the 14 days treatment, there was no significant difference between the treatment group and the control group, both for the rosiglitazone N-demethylase enzyme activity or the changed of rat body weight.
Validation and Quantification of Domperidone in Spiked Plasma Matrix Using Reversed Phase HPLC-UV Method Sekar Ayu Pawestri; Akhmad Kharis Nugroho; Endang Lukitaningsih; Purwantiningsih Purwantiningsih
Indonesian Journal of Chemistry Vol 21, No 5 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.64253

Abstract

Pharmacokinetics studies of domperidone generally analyze plasma matrix samples. The present work aimed to develop and validate a rapid and simple reversed phase-HPLC method for quantifying domperidone in plasma matrices. The chromatographic method implemented: 1. Luna Phenomenex® C18 (250 mm × 4.6 mm i.d; 5 µm) column, 2. isocratic mobile phase mixture of phosphate buffer 0.02 M:acetonitrile (70:30, v/v) with a flow rate of 1 mL/min, 3. UV detection at 285 nm. Domperidone and propranolol hydrochloride (as internal standard) were extracted from the deproteinated plasma sample. The method linearity was 0.998 in the range concentration of 15–200 ng/mL. The percentage of accuracy error was between -8.49–4.31%, while the percentage coefficient variation of precision ranged between 5.11–14.24%. This proposed method was simple, rapid (separation time less than 10 min), and selective. The validation parameters responses satisfied the method's requirements to determine domperidone in a plasma sample.
Challenges and potentials of developing domperidone into the transdermal delivery Sekar Ayu Pawestri; Akhmad Kharis Nugroho; Endang Lukitaningsih; Purwantiningsih Purwantiningsih
Majalah Farmaseutik Vol 18, No 3 (2022)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v18i3.74154

Abstract

Nausea, which often coexists with vomiting, is an uneasy sensation in the stomach caused by several factors. Oral domperidone is widely prescribed in nausea treatment. The low bioavailability of oral domperidone makes a patient take the drug more frequently, even though some patients have difficulty swallowing the drug when suffering nausea. Recently, the drug formulation development for transdermal delivery systems is expected to increase in the future. Practical and increase patient comfortable is the notable advantages of using transdermal dosage form. Domperidone is currently being studied into various pharmaceutical dosage forms with the transdermal route. This present article provides summaries of the constraints also the current formulation development of domperidone which shows the potential of domperidone in transdermal delivery.
Penyesuaian Dosis Obat Pasien Penyakit Ginjal Kronik di RSUD Kardinah Tegal Nur Amalia Rosyada; Purwantiningsih Purwantiningsih; Tri Murti Andayani
Majalah Farmaseutik Vol 19, No 2 (2023)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v19i1.75249

Abstract

Adanya gangguan pada ginjal dapat menyebabkan akumulasi obat dan dapat menginduksi nefrotoksisitas. Hal ini dapat dihindari dengan pemilihan dan penyesuaian dosis obat yang tepat untuk memastikan luaran klinik yang optimal dan mencegah terjadinya efek samping obat. Penelitian ini dilakukan dengan tujuan melihat prevalensi kesesuaian dosis obat pada pasien Penyakit Ginjal Kronik (PGK) dan hubungan kesesuaian dosis obat dengan luaran klinik di Rumah Sakit Umum Daerah (RSUD) Kardinah. Penelitian ini menggunakan metode observasional analitik dengan rancangan kohort dan pengambilan data secara retrospektif. Subjek penelitian adalah pasien PGK rawat inap di RSUD Kardinah periode tahun 2019, data diperoleh dari rekam medik. Perhitungan estimasi laju filtrasi glomerulus menggunakan formula Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) dan kesesuaian obat dibandingkan dengan pustaka dan formula Giusti Hayton. Analisis data statistik dalam penelitian ini menggunakan chi square test untuk mengetahui hubungan antara kesesuaian dosis obat dengan luaran klinik pasien PGK di RSUD Kardinah. Analisis data multivariat menggunakan multiple logistic regression untuk melihat hubungan variabel perancu dengan luaran klinik. Hasil penelitian menunjukkan dari 84 rekam medik sejumlah 829 obat diresepkan, 427 obat (51,5%) diantaranya memerlukan penyesuaian dosis. Dari 427 obat tersebut, obat yang sesuai dosis sebanyak 376 obat (88%) dengan luaran klinik membaik 336 obat (89%). Tidak terdapat hubungan yang bermakna antara kesesuaian dosis dengan luaran klinik (RR=1,222, 95% CI=0,994-1,503, p=0,074). Variabel perancu yang mempengaruhi luaran klinik adalah hemodialisis (RR = 4,643, 95% CI=1,11-19,425, p=0,036).
Co-Authors Abas Hj Hussin Akhmad Kharis Nugroho Charles S. Vairappan Datin Maryati Mohd. Endang Lukitaningsih Endang Lukitaningsih Erna Prawita Setyowati Indah Purwantini, Kit Lam Chan Lukman Hakim Lukman Hakim Marchaban Marchaban Nur Amalia Rosyada Oka Samara Oktavia Eka Puspita Retno Murwanti Sekar Ayu Pawestri Sekar Ayu Pawestri Silvia Utami Tunjung Pratiwi Subagus Wahyuono Tri Murti Andayani Vera Fauziati