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All Journal VALENSI INDONESIAN JOURNAL OF PHARMACY ANNALES BOGORIENSES
Asrul Muhamad Fuad, Asrul Muhamad
Research Center for Biotechnology, Indonesian Institute of Sciences JL. Raya Jakarta - Bogor Km.46 Cibinong 16911 Bogor - Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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Journal : INDONESIAN JOURNAL OF PHARMACY

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The Effect Of Temperature On Recombinant Human Granulocyte Colony Stimulating Factor Production By Pichia pastoris Expression System Adiredja, Yuliawati; Fuad, Asrul Muhamad
Indonesian Journal of Pharmacy Vol 29 No 2, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1286.552 KB) | DOI: 10.14499/indonesianjpharm29iss2pp94

Abstract

Granulocyte colony stimulating factor (G-CSF) is a haematopoetic growth factor that functions as specific stimulator of the survival, proliferation, and differentiation of precursor cells of the neutrophilic granulocyte cell lineage as well as an activator of mature neutrophil function. The main objective of this work is to compare the effect of different temperature on the production of extracellular recombinant G-CSF in Pichia  pastoris. Cells were cultured for 72h in baffled shake-flasks at 20°C, 25°C, and 30°C in two different medium; buffered glycerol/methanol-complex medium (BMGY/BMMY) and buffered minimal glycerol/methanol (BMGH/BMMH) after methanol induction every 12h. Expressed recombinant hG-CSF in the methylotrophic yeast P. pastoris was analyzed with SDS-PAGE. The 23 kDa protein was secreted into the culture supernatant when induced with methanol. Production of recombinant G-CSF protein in P. pastoris at 30°C at 48h incubation after methanol induction every 12h is the highest in both complex and minimum medium.
Co-Authors Adiredja, Yuliawati Agustiyanti, Dian Fitria Akbar, Nadhira Fathiaz Anak Agung Gede Sugianthara Aryani, Hanifah DEBBIE SOFIE RETNONINGRUM DESSY NATALIA Febriana Dwi Wahyuni Handayani, Ira HENI RACHMAWATI Kusharyoto, Wien LAKSMI AMBARSARI Martha Sari Retno Damayanti Soejoedono Suharsono Suharsono TUTUS GUSDINAR