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All Journal Bioscience Biogenesis: Jurnal Ilmiah Biologi BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI ANNALES BOGORIENSES Jurnal Pengabdian Masyarakat AbdiMas Biofarm : Jurnal Ilmiah Pertanian SPIN: Jurnal Kimia & Pendidikan Kimia Jurnal Pengabdian kepada Masyarakat
Febriana Dwi Wahyuni
Universitas Esa Unggul
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Social Sciences Other

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Constitutive Expression of Candida antarctica Lipase B (CALB) in Pichia pastoris Using pGAPZα Vector Wahyuni, Febriana Dwi; Fuad, Asrul Muhamad; Suharsono, Suharsono
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.586 KB) | DOI: 10.14203/ab.v20i1.225

Abstract

The CalBsyn gene was previously constructed synthetically to encode Candida antarctica lipase B (CALB). Lipase from CalBsyn gene is slightly different from that of wild type CALB (CALB-wt) where it has three amino acids substitutions at different positions, i.e. V210I, A281E, and V221D, to improve enzyme’s thermostability and catalytic efficiency. The CalBsyn gene was isolated from pJ912-CalBsyn vector by digestion using XhoI restriction enzyme. The CalBsyn gene then was ligated to pGAPZα expression vector and transformed into E. coli TOP10F’ in order to obtain recombinant vector pGAPZα-CalBsyn. The result showed that pGAPZα-CalBsyn recombinant vector was successfully transformed into E. coli TOP10F’ with transformation efficiency of 4.11 x 103 cfu/µg plasmid DNA. The pGAPZα-CalBsyn recombinant plasmid was successfully introduced into Pichia pastoris SMD1168H using electroporation method with transformation efficiency of 1.01 x 102 cfu/µg DNA. Qualitative lipase activity assays showed that transformed P. pastoris secreted recombinant lipase (CALB) and has lipolytic activity; while quantitative lipase activity assays showed that the lipase activity was 63.5 Units/ml in 48 hours. Analysis using SDS-PAGE showed that CALB protein was expressed successfully and the recombinant protein’s molecular size was approximately 45 kDa.
PENINGKATAN PENGETAHUAN SISWA SMA NEGERI 1 GLAGAH TENTANG KULTUR JARINGAN MELALUI EDUKASI ONLINE Wahyuni, Febriana Dwi; Novianti, Titta
Jurnal Pengabdian Masyarakat AbdiMas Vol 8, No 03 (2022): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v8i03.5213

Abstract

Kultur jaringan merupakan bagian dari mata pelajaran biologi di sekolah menengah atas jurusan IPA. Kegiatan pengabdian kepada masyarakat ini merupakan salah satu langkah awal untuk memperkenalkan dan meningkatkan wawasan siswa Sekolah Menengah tentang kultur jaringan. Kultur jaringan adalah salah satu metode perbanyakan tanaman yang dilakukan dengan mengisolasi bagian dari tanaman dan ditumbuhkan pada media buatan dalam kondisi steril dan terkontrol sehingga nantinya bisa tumbuh menjadi tanaman yang utuh. Adapun kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan pengetahuan tidak hanya kepada siswa, tetapi juga kepada guru di lingkungan SMA Negeri 1 Glagah mengenai teknik-teknik kultur jaringan. Adapun metode yang digunakan untuk penyampaian materi tersebut adalah metode ceramah dan diskusi. Hampir semua siswa (88,89%) menilai bahwa kegiatan ini sangat menarik dan materi yang diberikan mudah dipahami. Hampir separuh siswa menyatakan bahwa materi yang diberikan sangat bermanfaat. Setelah adanya kegiatan ini, para siswa dan guru biologi memahami pengetahuan tentang kultur jaringan. Penguasaan tentang kultur jaringan ini diharapkan dapat menjadikan siswa sebagai individu yang kreatif dalam mengimplementasikan teknik-tekniknya. Kata kunci : biologi, bioteknologi, kultur jaringan, pertanian
PENINGKATAN PENGETAHUAN MASYARAKAT TENTANG TANAMAN TRANSGENIK MELALUI EDUKASI ONLINE Wahyuni, Febriana Dwi; Novianti, Titta; Saraswati, Henny; Seprianto, Seprianto
Jurnal Pengabdian Masyarakat AbdiMas Vol 8, No 01 (2021): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v8i01.4785

Abstract

Transgenic plants are genetically modified plants made by inserting one or a number of genes from other organisms, with the aim of obtaining superior and desirable new traits, such as resistance to drought stress, resistance to pests, resistance to herbicides. So far, there are still many people who are worried about the safety of transgenic plants that have been successfully marketed. Therefore, we strive to provide information based on existing references to increase public understanding of GM crops. The educational materials provided consisted of an introduction to plant breeding techniques and the purpose of genetic transformation in plants, gene cloning techniques and the transformation of target genes into plant genomes to produce transgenic plants and molecular techniques used to select transgenic plants which were delivered in the form of online seminars. via zoom and live streaming on youtube. The flow of activities is divided into two stages, namely material presentation and discussion. This activity is expected to increase knowledge about assembling techniques for transgenic plants as an effort to educate the public regarding the safety of transgenic plants. Keywords: biotechnology, cloning, GMOs, transgenic plants
PENGENALAN BIOTEKNOLOGI DAN METODE KULTUR JARINGAN SEBAGAI UPAYA PENINGKATAN WAWASAN SISWA DI SMA YAYASAN PERSIAPAN GENERASI BARU Wahyuni, Febriana Dwi; Novianti, Titta; Saraswati, Henny; Seprianto, Seprianto
Jurnal Pengabdian Masyarakat AbdiMas Vol 6, No 3 (2020): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v6i3.3333

Abstract

AbstractHigh school students is a level of secondary school education that will enter a higher level of education in tertiary institutions. Of course, a lot of preparation must be done as a provision after graduating, both knowledge and skills. This community service activity is one of the first steps to introduce and enhance secondary school students' insights about biotechnology. Biotechnology is a relatively new study program in Indonesia. Biotechnology has many focus areas that can be studied, including tissue culture and microbiology. The community service activity aims to provide knowledge not only to students, but also to teachers in the Senior High School Foundation Preparation Foundation regarding tissue culture and microbiology techniques. The method used for the delivery of the material is the lecture and practicum methods. Mastery of tissue culture and microbiology is expected to make students as creative individuals in creating a product that is related to these two focus areas. Keywords: biotechnology, tissue culture, microbiology AbstrakSiswa SMA merupakan tingkatan dari pendidikan sekolah menengah yang akan memasuki tingkatan pendidikan yang lebih tinggi di perguruan tinggi. Tentunya banyak persiapan yang harus dilakukan sebagai bekal setelah lulus nantinya, baik ilmu maupun keterampilan. Kegiatan pengabdian kepada masyarakat ini merupakan  salah satu langkah awal untuk memperkenalkan dan meningkatkan wawasan siswa Sekolah Menengah tentang bioteknologi. Bioteknologi merupakan salah satu program studi yang terhitung baru di Indonesia. Bioteknologi mempunyai banyak bidang fokus yang bisa dipelajari, diantaranya yaitu kultur jaringan dan mikrobiologi. Adapun kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan pengetahuan tidak hanya kepada siswa, tetapi juga kepada guru di lingkungan SMA Yayasan Persiapan Generasi Baru mengenai teknik-tekniik kultur jaringan dan mikrobiologi. Adapun metode yang digunakan untuk penyampaian materi tersebut adalah metode ceramah dan praktikum. Penguasaan tentang kultur jaringan dan mikrobiologi ini diharapkan dapat menjadikan siswa sebagai individu yang kreatif dalam menciptakan suatu produk yang berkaitan dengan kedua bidang fokus tersebut. Kata Kunci : bioteknologi, kultur jaringan, mikrobiologi
PKM PENINGKATAN MUTU PANGAN LOKAL BERBASIS PANGAN FERMENTASI DI PULAU PAYUNG, KEPULAUAN SERIBU Seprianto, Seprianto; wahyuni, Febriana Dwi
Jurnal Pengabdian Masyarakat AbdiMas Vol 6, No 2 (2020): JURNAL PENGABDIAN MASYARAKAT ABDIMAS
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v6i2.3186

Abstract

AbstractThe Thousand Islands Region is administratively included in DKI Jakarta Province but has different characteristics from other DKI Jakarta areas. Payung Island is one of the islands in the Thousand Islands which is a marine tourism destination that is quite crowded with tourists. The diversity of processed products, especially fish-based products, is still little developed on this island, so there are not many choices for tourists. The purpose of this activity is to develop diversified food products by improving the quality of fermented-based local food. Through the cooperation of UMKMBarakuda Payung Island partners, the implementation of community service is going well. The method of implementation is by presentation and direct practice such as making peda fish, while other processed products such as bekasang and fish sauce were only a video documentary. A total of 13 members of the Barakuda group participated in this training. Processed products from fish have not been developed much by UMKM Barakuda, Payung Island. This activity also collaborates with the Product Design Team, so that it can provide an overview of the product from manufacturing to the packaging stage. It is expected that the implementation of this activity will increase the role of the community to be creative, innovative, independent, and entrepreneurial so that they can actively participate in regional and national development and become an attraction for tourists to visit the Thousand Islands. Keywords :Payung island, local food, fermentation food AbstrakWilayah Kepulauan Seribu secara administratif masuk ke dalam Propinsi DKI Jakarta, namun memiliki  karakteristik yang berbeda dengan wilayah DKI Jakarta lainnya. Pulau Payung merupakan salah satu pulau di Kepulauan Seribu yang menjadi destinasi wisata bahari yang cukup ramai dikunjungi oleh wisatawan.Keberagaman produk olahan terutama berbahan dasar ikan masih sedikit dikembangkan di pulau ini, sehingga tidak banyak pilihan bagi wisatawan.Tujuan kegiatan ini adalah untuk pengembangan diversifikasi produk pangan dengan meningkatkan mutu pangan lokal berbasis fermentasi.Melalui kerjasama mitra UMKM Barakuda Pulau Payung, pelaksanaan pengabdian masyarakat ini berjalan dengan baik.Metode pelaksanaannya dengan presentasi dan praktek langsung seperti pembuatan ikan peda, sedangkan produk olahan lainnya seperti bekasang dan kecap ikan hanya berupa video dukumenter.Sebanyak 13 anggota kelompok UMKM Barakuda mengikuti pelatihan ini.Produk olahan dari ikan tersebut belum banyak dikembangankan oleh UMKM Barakuda Pulau Payung.Kegiatan ini juga berkalaborasi dengan Tim Desain Produk, sehingga dapat memberikan gambaran produk mulai dari pembuatan sampai pada tahap pengemasannya. Diharapkan dari pelaksanaan kegiatan ini akan meningkatan peranan masyarakat untuk kreatif, inovatif, mandiri, serta  berwirausaha sehingga mampu berpartisipasi aktif dalam pembangunan daerah dan nasional dan menjadi daya tarik wisatawan untuk berkunjung ke Kepulauan Seribu. Kata kunci:Pulau payung, pangan lokal, pangan fermentasi
PENINGKATAN WAWASAN BIOTEKNOLOGI SISWA SMK NEGERI 9 KOTA TANGERANG MELALUI KEGIATAN PRAKTIKUM ISOLASI DNA TANAMAN Wahyuni, Febriana Dwi
Jurnal Pengabdian Masyarakat AbdiMas Vol 5, No 4 (2019): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v5i4.2788

Abstract

AbstractBiotechnology is one of the fields of Biology that must be mastered by students for Biotechnology which is directly related to daily life. Learning biotechnology needs to be supported by laboratory activities as a form of proof of the existing theory. general biotechnology material. The problem that addresses partners is the problem of knowledge about biotechnology as one of the materials discussed in biology and there are no practical activities that support in the field of biotechnology. As for the purpose of community service activities is to improve students' insight into biotechnology and improve student skills in the laboratory through practicing plant DNA isolation. From this activity, students are expected to have basic skills in DNA isolation as a provision for the development of other skills in the field of biotechnology. From the results of feedback given to students, it is acceptable for 52% of students to strongly agree on the material presented, and received 48% of students quite understanding the material presented. The participants were also interested in training related to biotechnology. The results of student feedback can be known to most students who need training on Bacterial Inhibition Test (68%). Then as many as 23% asked for practicum about cloning, 8% asked for practicum for DNA bands, and 1% asked for observations about animal DNA isolation. Based on the activities that have been carried out, it can be concluded that students gain knowledge and improve their insight and skills in the field of biotechnology through DNA isolation activities. Keywords: biotechnology, practicum, DNA isolation AbstrakBioteknologi merupakan salah satu bidang ilmu dalam biologi yang harus dikuasai oleh siswa karena bioteknologi banyak terkait langsung dengan kehidupan sehari-hari.Pembelajaran bioteknologi perlu didukung kegiatan laboratorium sebagai bentuk pembuktian dari teori yang ada.Penelitian terdahulu membuktikan bahwa secara umum siswa Sekolah Menengah belum memahami secara umum materi bioteknologi.Permasalahan yang dihadapi mitra adalah kurangnya pengetahuan tentang bioteknologi sebagai salah satu materi yang dipelajari dalam pelajaran biologi dan belum ada kegiatan praktikum yang mendukung dalam mempelajari bioteknologi.Adapun tujuan dari kegiatan pengabdian kepada masyarakat ini adalah untuk meningkatkan wawasan siswa tentang bioteknologi dan meningkatkan keterampilan siswa di dalam laboratorium melalui kegiatan praktikum isolasi DNA tanaman.Dari kegiatan ini diharapkan para siswa memiliki keterampilan dasar dalam isolasi DNA sebagai bekal untuk pengembangan keterampilan lainnya di bidang bioteknologi. Dari hasil feedback yang diberikan kepada siswa, dapat diketahui bahwa 52% siswa sangat memahami tentang materi yang telah disampaikan, dan sisanya 48% siswa cukup memahami materi yang disampaikan. Para peserta juga menginginkan adanya praktikum lainnya yang berkaitan dengan bioteknologi.Dari hasil feedback siswa dapat diketahui bahwa sebagian besar para siswa menginginkan adanya pelatihan tentang Uji Penghambatan Bakteri (68%). Kemudian sebanyak 23% menginginkan praktikum tentang kloning, 8% menginginkan praktikum pengamatan pita DNA, dan 1% menginginkan pengamatan tentang isolasi DNA hewan. Berdasarkan kegiatan yang telah dilakukan, dapat disimpulkan bahwa para siswa memperoleh pengetahuan dan peningkatan wawasan dan keterampilan di bidang bioteknologi melalui kegiatan isolasi DNA. Kata kunci: bioteknologi, praktikum, isolasi DNA
Candida antarctica Lipase B Synthetic Gene: A Bioinformatics Analysis Febriana Dwi Wahyuni; seprianto Seprianto
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.901 KB) | DOI: 10.24036/0201822100216-0-00

Abstract

Enzyme exploration is important to support the development of biotechnology. To facilitate microbial discovery, it can be done synthetically using bioinformatics methods. Candida antarctica lipase B (CALB) is one of enzyme derived from microorganisms and has been applied as a biocatalyst in several industry. The methods used in this study are the analysis of gene structure using NCBI, analysis of protein CALB sequence using Uniprot, analysis of 3D protein structure using Swiss model, analysis primary design using primer3 and analysis of restriction sites using snapgene. The construction of synthetic calB gene obtained based on the results of the CalB gene sequence in geneBank using NCBI with access number Z30645.1. CalB gene wildtype is modified by adding several restriction enzyme (XhoI, XbaI, ClaI, and BglI), 6 Histags, and 2x stops codon and produce 1083 base pairs. CALB protein has 342 amino acids. Based on 3D structure, CALB protein has three molecules in common with one homotrimer ring that will encircle the double helix DNA. Primers used for calB gene amplification are CALB forward 5'-TCCCCAGTATCAGGTCCAAG-3 ' and CALB reverse 5'-GACACCTGAGGCTGAACGAT-3'.
Bioinformatics Analysis to Construct Cellulose-binding Module Synthetic Gene and Design Primer Febriana Dwi Wahyuni; Asrul Muhamad Fuad
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13204

Abstract

Cellulose-binding module (CBM) is a protein domain commonly found in various types of cellulase enzymes. The function of this CBM can be used for the binding process and the immobilization of a protein in the cellulose matrix. CBM can be obtained from several organisms, one of them is Trichoderma reesei. To get a gene, it does not have to be isolated from the original organism. Gene sequences can be obtained synthetically through bioinformatics analysis in accordance with the same gene sequences as those at Gene Bank. Bioinformatics analysis can be used to find new gene sequences or existing genes. This study aims to get a cbmsyn synthetic gene quickly and efficiently without reducing protein activity, which can then be ligated with other genes so that it functions as an immobilized enzyme. From the results of bioinformatics analysis, obtained DNA sequences measuring around 498 pb with 166 amino acid protein lengths. The sequence was modified by adding several restriction sites, namely BamHI, AfeI, and ScaI. The DNA sequences obtained were optimized with the Pichia pastoris codon.
SUBSTITUTION OF SYNTHETIC HORMONES WITH ORGANIC MATERIALS ON THE GROWTH OF ORCHID PLANTS (Phalaenopsis amabilis) AS A GROWTH REGULATORY SUBSTANCE IN VITRO Febriana Dwi Wahyuni; Kevin Febrianus Moda; Dimas Ridho Irvansah; Novita Sari
BIOEDUKASI Vol 19 No 2 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i2.27141

Abstract

In Indonesia, the agribusiness sector has begun to expand due to the increase in entrepreneurship of orchid plants. Several genera and species of orchid plants are promising to be traded as ornamental plants, one of which is the moon orchid (Phalaenopsis amabilis). However, the limited number of seeds or plants produced by conventional propagation methods takes a long time to get new tillers. Therefore, tissue culture techniques can be used to grow and reproduce moon orchid plants. In the success of in vitro culture, the balance of growth regulators is an essential factor. On the other hand, synthetic hormones such as 1-naphthalenaecetic acid (NAA) and 6-benzylaminopurine (BAP) are relatively expensive. The study in this research is to substitute the synthetic hormones NAA and BAP with organic ingredients that are cheap and easy to find. Such as Purple Sweet Potatoes and Beans and find out the right concentration of organic matter. This study used a completely randomized design with two factorials (concentration of purple sweet potato and green beans) with three repetitions. PLB (Protocorm Like Bodies) moon orchid (Phalaenopsis amabilis) was treated with: positive control (2 ppm), negative control (without the addition of organic or synthetic hormones), and variations in the concentration of purple sweet potato and green beans organic matter. The result was that C4D0 (500g/L purple sweet potato extract and 0 g/L green bean extract) was an excellent formulation, although there was no significant interaction between purple sweet potato and green bean extracts against moon orchid.
In-Silico Analysis for cryI Gene Amplification from Bacillus thuringiensis Febriana Dwi Wahyuni; Henny Saraswati; Kartika Sari Dewi
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.16430

Abstract

Abstract Bacillus thuringiensis is one type of bacteria that has been used as a microbiological control agent for pests and a vector of plant disease. The presence of Cry proteins inside the B. thuringiensis can be acted as a specific insect repellent that only toxic to certain insects. The CryI protein is toxic to Lepidoptera insects which can attack various types of plants. Polymerase Chain Reaction (PCR) is a common method that can be used to amplify the gene encoding CryI proteins from B. thuringiensis. This research aimed to design a good primer candidate for cryI gene amplification from B. thuringiensis. In silico analysis for designing cryI primer was carried out using some software, such as BLAST for searching cryI gene sequence, Bioedit for sequences alignment, and DINAmelt for analyzing dimer structure of primers. Ten primer candidates were successfully obtained based on the result of the primer3 software. A pair of primer was selected to amplify the cryI gene, with forward primer 5’- CGGTGAATGCCCTGTTTACT -3’ and reverse primer 5’-CGGTCTGGTTGCCTATTGAT -3’. Amplification of the cryI gene by PCR method using selected primer resulting in a PCR product with a length of approximately 200 bp.
Co-Authors Adri Nora alfero Putra Iryanto Anisa Febriyanti Asrul Muhamad Fuad Asrul Muhamad Fuad Asrul Muhamad Fuad, Asrul Muhamad callista marsya Dedi Supriadi Dimas Ridho Irvansah Enung Sri Mulyaningsih feby feby Hani Fitriani, Hani Henny Saraswati Henny Saraswati Iis Nur Asyiah Kartika Sari Dewi Kevin Febrianus Moda Meisya Then Septian Muhamad Amza Sidiq nie nie Ningsih, Irnawati Novianti, Titta Novita Sari Oktaviani Naulita Turnip putri mega utami Rahman, Nurhamidar roaslein putri Roselein Putri sabar pambudi Saraswati, Henny Saraswati, Henny Seprianto, Seprianto Slamet Hariyadi Sri Rianawati Suharsono Suharsono Suharsono Suharsono Syafruddin Ilyas