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Journal : Research Journal of Life Science

Fungal Endophytic Beauveria bassiana in Chinese Kale Against Plutella xylostella (L.) Larvae Afandhi, Aminudin; Ikawati, Silvi; Baroro, Ismatul; Afiyanti, Mufidah; Sari, Rose Novita; Handoko, Handoko; Suyono, Tri; Setiawan, Yogo; Tarno, Hagus
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.4

Abstract

This study evaluated three methods of inoculation of Beauveria bassiana endophytes from Chinese kale and then infected into Plutella xylostella L to determine the mortality rate. The endophytic B. bassiana using three different inoculation methods including seeds-soaking, leaf-spraying, and soil-wetting. The Chinese kale seeds from Winsa variety, B.bassiana isolates derived from collection planthopper. The endophytic fungus was identified to be B. bassiana based on the analysis of colony morphology. The results of this study included the degree of colonization of Chinese kale plants and the mortality of P. xylostella. The average B. bassiana colonies in leaves were 13.89%, stems 2.77% and roots 5.55%. The results showed on leaves that the seeds-soaking method obtained higher colonization of B. bassiana rate when compared to leaf-spraying and soil-wetting. The highest percentage of P. xylostella mortality was generated from seeds-soaking (45%), soil-wetting (37.5%), leaf-spraying (35%). The B. bassiana fungus which caused the fastest LT50 with a seeds-soaking (409.48 hours), and then soil-wetting (679.15 hours), leaf-spraying (1090.21 hours). The positive effects of seeds-soaking of endophytic B. bassiana and mortality of Plutella xylostell.
Abundance and Diversity of Fruit Flies Species (Diptera: Tephritidae) in Bogor and Depok District Octavia, Evy; Tarno, Hagus; Himawan, Toto; Setiawan, Yogo
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.7

Abstract

Fruit flies (Diptera: Tephritidae) are important pests of horticultural crops. This study investigates the abundance and species richness of Bogor and Depok District as a center area horticulture commodity based on Cue Lure Trap. Each Cue Lure Trap was observed to record the abundance and species richness of fruit flies at seven days intervals from June until August 2021 in Bogor District (Babakan, Mekarsari and Parigi Mekar) and Depok District (Bedahan and Pancoran Mas), West Java, Indonesia. A total of 1,025 individuals were collected and consist of nine species of fruit fly i.e., Bactrocera albistrigata, B. carambolae, B. neocognata, B. verbascifoliae, Dacus conopsoides, D. longicornis, Zeugodacus calumniata, Z. caudatus, and Z. cucurbitae. The number of individuals of fruit flies was highest in the Bedahan, Depok District (F = 5.454, P = 0.002). Zeugodacus cucurbitae was the most abundant species (792 individuals), representing 77.27% of the specimens collected during the sampling period. The highest Shannon–Wiener diversity index of all fruit flies captured in this study was in Bedahan, Depok (1.11) and the lowest in Parigi Mekar, Bogor (0.23). A Bray–Curtis analysis showed that the species compositions of fruit flies on Bedahan and Parigi Mekar had a similarity of 70.1%.