Retno Dyah Puspitarini
Jurusan Hama Dan Penyakit Tanaman, Fakultas Pertanian, Universitas Brawijaya

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BIOLOGICAL STUDY OF TWO SPOTTED SPIDER MITE Tetranychus sp. (ACARI: TETRANYCHIDAE) ON THREE LEAF PHASES OF MUNG BEAN AND ADZUKI BEAN FOR MITE MASS REARING Puspitarini, Retno Dyah; Satria, Arif Danu; Astuti, Ludji Pantja
AGRIVITA, Journal of Agricultural Science Vol 35, No 3 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Two-spotted spider mite (TSM) Tetranychus sp. is one of prey mites for phyotoseiid mite mass rearing.  Based on previous research that TSM population developed well on Mung bean (Vigna radiata) and Adzuki bean (V. angularis) as host plants, confirmed growth and development of TSM on both beans. Research was aimed to observe biology of TSM on three phases of Mung bean and Adzuki bean leaf such as at primary leaf unrolled completely (V1), at the 1st trifoliate unrolled completely (V2), and at the 2nd trifoliate unrolled completely (V3). Pre adult stadia, life cycle, male and female longevity, female fecundity of TSM, and trichome density were observed on petri dishes contained each leaf phase of beans.  Result showed that three leaf phases of beans did not influence significantly in all biological variables, except fecundity. TSM fecundity was the highest on V3 phase of Mung bean (86.90). Trichome density on Mung bean leaf surface per mm² was lower than Adzuki bean. Trichome density of upper leaf surface of V3 phase of Mung bean (10.53) and Adzuki bean  (3.07) were lower than V1 (13.20; 12.40) and V2 phase (6.20; 5.27). V3 phase of Mung bean was most suitable for TSM mass rearing.Key words:  fecundity, leaf phase, mass rearing, trichome density, two-spotted spider mite   
Comparison of Five Legumes as Host of Tetranychus sp. (Acari: Tetranychidae) Mass Rearing Puspitarini, Retno Dyah; Qomariyah, Nurul; Afandhi, Aminudin
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Two spotted mite (TSM) Tetranychus sp. is often used as host for predator mite Phytoseiidae mass rearing. To obtain high population of TSM in short time, it must be reared in host plants that support optimum growth and development. The objective of this research was to observe population development of TSM on legumes such as mungbean (Vigna angularis), adzukibean (V. radiata), snapbean (Phaseolus vulgaris) and soybean (Glycine max) (wilis and anjasmoro cultivars) and to compare the growth of the legumes based on their fresh leaf weight and leaf area, and cost seed index (CSI). The results showed that mungbean was suitable host plant for TSM mass rearing because its population on this legume was the highest. Mungbean’s fresh leaf weight and leaf area was the highest. If TSM is reared in plant in greenhouse, CSI will become one of criteria for determining the host plant. Although CSI of mungbean was low because TSM population that was reared on it is the highest, the mungbean was the most suitable host plant for TSM growth and development. Another host plant that was suitable for TSM reared was adzukibean.Keywords: legumes, mass rearing, Tetranychus uritcae
EFFECT OF NATURAL HABITAT ON DIVERSITY OF HEMIPTERAN PREDATOR IN OIL PALM PLANTATION Rizali, Akhmad; Himawan, Toto; Fitriani, Ima; Rahardjo, Bambang Tri; Karindah, Sri; Puspitarini, Retno Dyah; Sahari, Bandung
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.923 KB) | DOI: 10.23960/j.hptt.11875-82

Abstract

Effect of Natural Habitat on Diversity of Hemipteran Predator in Oil Palm Plantation. Utillization of natural enemies in controlling oil palm pests still encounters obstacles, in particular, how natural enemies can survive and establish in oil palm plantations. The existence of natural habitats around oil palm plantations can allegedly support the occurence of natural enemies. The objective of this study was to investigate the effect of the presence of natural habitats on diversity of hemipteran predators in oil palm plantations. Field research was conducted in oil palm plantations located in Pangkalan Bun, Central Kalimantan. The plots were oil palm plantations with different distances from natural habitat i.e. near (less than 200 m), medium (about 2 km) and far (about 5 km). Each plot was selected six trees for fogging using insecticide with active ingredient lamda cyhalothrin. Based on research result, diversity of hemipteran predator in oil palm plantion was found 19 species belong to 2 families. The most dominant hemipteran predator was Sycanus sp., Eocanthecona sp. and Reduviidae sp5. The distance of oil palm plantation from natural habitat did not affect species richness, abundance and composition as well as recolonization of hemipteran predator in oil palm plantation. Abundance of hemipteran predator tend to decrease in different observation time, except Sycanus sp. Sycanus sp was likely has ability to rapidly recolonized and their occurrence were influenced by flowering vegetation in oil palm plantation.
Effects of Compost on Nematode Pratylenchus sp. (Tylenchida: Pratylenchidae) Population in Patchouli Subarjah, Cecep; Himawan, Toto; Puspitarini, Retno Dyah
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.07

Abstract

Patchouli is seen as one of the most important export commodities in Indonesia. The presence of parasitic nematode Pratylenchus sp. creates a barrier to plant cultivation. Increasing organic content in soil by applying compost in the plantation is one of approaches in controlling the nematode. This research was mainly aimed to study the effects of compost fertilization on the population growth of nematode Pratylenchus sp. and yields on patchouli. These research used a Completely Randomised Design (CRD) consisting of 12 treatments with three replicates. Selected plant was inoculated with nematodes with the following initial population: 100, 200, 300, 400, and 500 nematodes in soil with and without compost. The plant with no inoculation was used as control. The result of the research confirmed that the addition of compost was capable of suppressing the population of nematode Pratylenchus sp. as much as 58.93 - 67.31% and 40.74 - 59.19% in root and in soil of each treatment, respectively. Compost applied in each treatment positively affected the increasing amount of Patchouli oil yielded, and it could also reduce the loss as much as 18.34 - 43.36%. Trichoderma sp. was found to be dominant in compost in the treatment with its population of 2.1 × 104 propogul/g.
Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Pramudi, Muhammad Indar; Puspitarini, Retno Dyah; Rahardjo, Bambang Tri
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA
KEANEKARAGAMAN DAN KEKERABATAN LALAT BUAH (DIPTERA: TEPHRITIDAE) DI KALIMANTAN SELATAN BERDASARKAN KARAKTER MORFOLOGI DAN MOLEKULAR (RAPD-PCR DAN SEKUENSING DNA) M Indar Pramudi; Retno Dyah Puspitarini; Bambang Tri Rahardjo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.254 KB) | DOI: 10.23960/j.hptt.213192-202

Abstract

Diversity and phylogeny of fruit fly (Diptera: Tephritidae) in South Kalimantan based on morphology and molecular (RAPD-PCR and DNA sequencing). Seven species of fruit fly was known by morphological identification. The fruit flies were found from  trapping with methyl eugenol and fruit collecting at all study sites in South Kalimantan. The results showed that as much as 17  plants were infected by fruit fly. Dendrogram based on morphological identification analyzed by using UPGMA with MEGA 4 program consisted in a group consisting of 5 sub-groups. Bactrocera carambolae and Bactrocera papayae of morphology were still a closely related fruit fly at 0.935. Whereas, based on RAPD result analized by UPGMA using 20 character of DNA based, showed that out of seven species consisted 2 groups, 1st group were B. umbrosa,  B. occipitalis and sub-group of B. latifrons. The second group consists of sub-groups B.carambolae, B. papaya, sub-group B. albistrigata and B. cucurbitae. The results of dendrogram from sequencing DNA fruit fly analysis comprised one of group and three sub-groups. The first sub-groups were B. papayae, B. carambolae, B. occipitalis, B.latifrons. The second subgroup were B. cucurbitae and B. umbrosa. While B. albistrigata separate but still one group with another fruit flies. The results of DNA sequencing showed that there were a homology of the seven species of the fruit fly i.e at 83 base pair / bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A ). The length of the base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons were respectively 615, 898, 570.969, 898 and 615 bp. The results of morphological analysis and RAPD methods showed difference in the distribution of groups and sub-groups. But based on morphologycal and DNA identification seven species of fruit flies found were all same as the genebank.
Suitable Combination Between Beauveria bassiana (Balsamo) Vuillemin and Four Plant Leaf Extracts to Control Spodoptera litura (Fabricius) Aminudin Afandhi; Vivi Renna Pratiwi; Mochammad Syamsul Hadi; Yogo Setiawan; Retno Dyah Puspitarini
AGRIVITA, Journal of Agricultural Science Vol 42, No 2 (2020)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v42i2.2678

Abstract

Lepidopteran pests such as S. litura might cause yield losses in many crops especially during pest outbreak. The combinations of microbial and botanical insecticides were expected to increase the effectivity in controlling the respected insect pest. This research aimed to evaluate the combination of plant extracts and B. bassiana to control S. litura. The compatibility of B. bassiana with different plant extracts such as Neem, Chinaberry, Mexican Sunflower and Lantana leaves was studied in the laboratory. The compatibility was evaluated based on B. bassiana colony growth, conidia density, conidia viability, and mortality of S. litura larvae. After 12 days after application (DAA), colony growth of B. bassiana was reduced by all plant extract treatments. For the mortality of S. litura, the combined mixture of B. bassiana and 0.25% Chinaberry extract resulted the maximum mortality rate (44%). After 12 and 15 DAA, the 0.25% Chinaberry showed the highest colony growth (3.93 and 4.37 cm). The most suitable treatment was B. bassiana with 0.25% Chinaberry that had the conidial density at 1.77 x 108 conidia/ml and conidial viability of 75.63% and can enhanced the mortality of S. litura larvae.
Notes on the Citrus Rust Mite, Phyllocoptruta oleivora (Ashmead), as a Major Pest of Citrus in Indonesia Retno Dyah Puspitarini; Otto Endarto
AGRIVITA, Journal of Agricultural Science Vol 43, No 3 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v43i3.2997

Abstract

Most citrus farmers in Indonesia believed that rust on citrus fruits resulted from insect attacks. This research aims to determine the cause of rust on citrus fruit and its damage intensity. The study was carried out in commercial citrus plantations in three provinces, on Citrus sinensis and C. reticulata plantations in East Java, C. reticulata and C. suhuensis in West Kalimantan and C. suhuensis in North Sumatra. An additional observation was also conducted to collect mite specimens on citrus plantations in Malang, East Java. Scanning Electron Microscope (SEM) and light microscope were used to observe and identify each collected sample. Results showed that the cause of rust-colored citrus fruit in three provinces is the citrus rust mite, Phyllocoptruta oleivora (Ashmead). It belongs to the family Eriophyidae with two pairs of legs with regular segmentation, five-rayed featherclaws, and a net pattern on prodorsum. The specific damage produced by this mite is rust-colored symptoms. Damage intensity of citrus rust mite in tree provinces averaging 32.6%, ranging from 30 to 40%, and ca. the mite attacks 90% of citrus fruits. Predaceous mites, predaceous insects, and entomo-acaropathogenic fungi are found on plantations.
BIOLOGICAL STUDY OF TWO SPOTTED SPIDER MITE Tetranychus sp. (ACARI: TETRANYCHIDAE) ON THREE LEAF PHASES OF MUNG BEAN AND ADZUKI BEAN FOR MITE MASS REARING Retno Dyah Puspitarini; Arif Danu Satria; Ludji Pantja Astuti
AGRIVITA, Journal of Agricultural Science Vol 35, No 3 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i3.252

Abstract

Two-spotted spider mite (TSM) Tetranychus sp. is one of prey mites for phyotoseiid mite mass rearing.  Based on previous research that TSM population developed well on Mung bean (Vigna radiata) and Adzuki bean (V. angularis) as host plants, confirmed growth and development of TSM on both beans. Research was aimed to observe biology of TSM on three phases of Mung bean and Adzuki bean leaf such as at primary leaf unrolled completely (V1), at the 1st trifoliate unrolled completely (V2), and at the 2nd trifoliate unrolled completely (V3). Pre adult stadia, life cycle, male and female longevity, female fecundity of TSM, and trichome density were observed on petri dishes contained each leaf phase of beans.  Result showed that three leaf phases of beans did not influence significantly in all biological variables, except fecundity. TSM fecundity was the highest on V3 phase of Mung bean (86.90). Trichome density on Mung bean leaf surface per mm² was lower than Adzuki bean. Trichome density of upper leaf surface of V3 phase of Mung bean (10.53) and Adzuki bean  (3.07) were lower than V1 (13.20; 12.40) and V2 phase (6.20; 5.27). V3 phase of Mung bean was most suitable for TSM mass rearing.Key words:  fecundity, leaf phase, mass rearing, trichome density, two-spotted spider mite   
Pengaruh pengelolaan agroekosistem terhadap kelimpahan kutu loncat jeruk Diaphorina citri Kuwayama (Hemiptera: Liviidae) M. Zuhran; Gatot Mudjiono; Retno Dyah Puspitarini
Jurnal Entomologi Indonesia Vol 18 No 2 (2021): Juli
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.18.2.102

Abstract

Asian citrus psyllid (ACP) Diaphorina citri Kuwayama is a serious problem in citrus because it transmits huanglongbing (HLB) disease. Agricultural practices without paying attention to the agroecosystem complexity often cause the agroecosystem more suitable for insect pests proliferation and less friendly to natural enemies. The research aims to study the effectiveness of agroecosystem management compared to conventional farming in the ACP control. The effects of both treatments on the diversity and abundance of ACP’s natural enemies as well as the incidence and severity of HLB were also studied. Agroecosystem management consists of preventive and responsive actions. Preventive action was carried out through a polyculture system, planting refugia and legume cover crop, providing beetle banks, applying lime, applying organic and inorganic fertilizers, citrus pruning intensively, maintaining drainage ditches, and mechanical weed control. Responsive action is short-term control when the abundance of ACP reaches the action threshold. Responsive action was carried out through biological or chemical control. Meanwhile, conventional farming is a series of conventional farming and pest control techniques commonly applied by farmers. Each treatment was applied in three orchards of Siam Orange cv. Pontianak Citrus nobilis Lour var. microcarpa Hassk. The results showed that agroecosystem management was more effective in controlling the ACP population so that its abundance was lower and less fluctuated than conventional farming. ACP’s natural enemies in agroecosystem management were also more diverse than conventional farming. The abundance of ACP’s natural enemies and the incidence and severity of HLB in the two treatments were not significantly different.