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All Journal BERITA BIOLOGI Asia Pacific Journal of Sustainable Agriculture, Food and Energy (APJSAFE)
Tri Rini Nuringtyas
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Energy Environmental Science

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The Immunomodulatory Activity of Leaves Extract of Gaharu Gyrinops versteegii (Gilg.) Domke on Macrophage Cell of Mice (Mus musculus L.) in vitro Assyafiya Salwa; Tri Rini Nuringtyas; Lisna Hidayati; Nastiti Wijayanti
BERITA BIOLOGI Vol 21, No 2 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i2.4136

Abstract

The immune response is divided into innate immune response and adaptive immune response. Macrophages are one of the immunity cells that work both in innate and adaptive immune response. The activities of secondary metabolites present in agarwood-producing plants allow the potential for natural immunomodulators so that the purpose of this study was to determine the potential G. versteegii extract leaves as immunomodulator and to examine the effect of G. versteegii leaves extract toward macrophage cells of mice (Mus musculus L.). The steps to be carried out in this study were the preparation of G. versteegii leaves extract with multi-level extraction, collection of M. musculus, cytotoxicity test with MTT assay, phagocytosis activity test of macrophages calculated by light microscopy (400X), and Griess assay for NO production of macrophages. The cytotoxicity test resulted an IC50 value of G. versteegii leaves extract was 183.45 µg/mL and the Imboost® as comparison solution is 20.12 µg/mL. This value used to determine the concentration of extract for phagocytosis activity test and NO production of macrophages test. The results showed that G. versteegii leaves extract has the potential as an immunomodulator and extract with 20 µg/mL concentration has the highest effect on phagocytosis activity and NO production. 
Expression of rice resistance gene OsNPR1 against bacterial leaf blight on black rice Cempo Ireng cultivar after salicylic acid treatment Ikhsan Maulana; Triyaningsih; Tri Rini Nuringtyas; Yekti Asih Purwestri
Asia Pacific Journal of Sustainable Agriculture, Food and Energy Vol. 9 No. 1 (2021): June 2021
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy Network (SAFE Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36782/apjsafe.v9i1.78

Abstract

Black rice is an alternative staple food better than white rice. It has lower carbs, but higher antocyanin compared to white rice. Nowadays, black rice consumption has increased, production needs to be increased to accomodate the demand. But, to our knowledge, there is lack of information about black rice resistance against biotic stresses, one of black rice cultivar is Cempo Ireng. This information needed for optimal Cempo Ireng cultivation. In this research, we determined chlorophyl content and analyzed OsNPR1 expression of three cultivars: Java14 (resistant control), Cempo Ireng, and IR64 (susceptible control), against Xanthomonas oryzae (Xoo). All of them were sprayed with salicylic acid (SA) before inoculated with Xoo. IR64 24h had the lowest chlorophyll content (0,576 ± 0,066 mg/g), meanwhile Java14 72h had the highest content (2,358 ± 1,301 mg/g). However, all the cultivars did not show any correlation between them and time after Xoo inoculation. OsNPR1 expression did not show any significant change in Java14 and Cempo Ireng after being inoculation. However, IR64 showed increasing OsNPR1 in 72h and did not change in 96h after inoculation. It indicated that OsNPR1 played important role in IR64 resistance against Xoo, but not in Cempo Ireng and Java14.
Co-Authors Assyafiya Salwa Ikhsan Maulana Lisna Hidayati Nastiti Wijayanti Triyaningsih Yekti Asih Purwestri