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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BERITA BIOLOGI
Lisna Hidayati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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The Immunomodulatory Activity of Leaves Extract of Gaharu Gyrinops versteegii (Gilg.) Domke on Macrophage Cell of Mice (Mus musculus L.) in vitro Assyafiya Salwa; Tri Rini Nuringtyas; Lisna Hidayati; Nastiti Wijayanti
BERITA BIOLOGI Vol 21, No 2 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i2.4136

Abstract

The immune response is divided into innate immune response and adaptive immune response. Macrophages are one of the immunity cells that work both in innate and adaptive immune response. The activities of secondary metabolites present in agarwood-producing plants allow the potential for natural immunomodulators so that the purpose of this study was to determine the potential G. versteegii extract leaves as immunomodulator and to examine the effect of G. versteegii leaves extract toward macrophage cells of mice (Mus musculus L.). The steps to be carried out in this study were the preparation of G. versteegii leaves extract with multi-level extraction, collection of M. musculus, cytotoxicity test with MTT assay, phagocytosis activity test of macrophages calculated by light microscopy (400X), and Griess assay for NO production of macrophages. The cytotoxicity test resulted an IC50 value of G. versteegii leaves extract was 183.45 µg/mL and the Imboost® as comparison solution is 20.12 µg/mL. This value used to determine the concentration of extract for phagocytosis activity test and NO production of macrophages test. The results showed that G. versteegii leaves extract has the potential as an immunomodulator and extract with 20 µg/mL concentration has the highest effect on phagocytosis activity and NO production. 
Phytochemical Analysis, Antioxidant, and Antibacterial Activity of Bidara laut (Strychnos lucida R.Br) Grown in West Bali National Park, Indonesia: Phytochemical Analysis, Antioxidant, and Antibacterial Activity of Bidara laut Krisnawati; Anita Apriliani Dwi Rahayu; Lisna Hidayati; Rizki Arisandi; Ganis Lukmandaru
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Strychnos lucida R.Br. is a medicinal plant believed to treat various diseases. The study aimed to identify the phytochemical compounds, total phenolic content (TPC), antioxidant, and antibacterial activity of the root, seed, stem, and leaf of S. lucida. Antioxidant activity was examined using the DPPH method in the methanol extract. The extract of four parts of S. lucida was tested against three bacteria (Bacillus subtilis Inacc-B334, Staphylococcus aureus Inacc-B4, and Escherichia coli Inacc-B5) using the disk diffusion method. The results showed that alkaloids and tannins were detected in all parts of the plant. The highest TPC was shown in root (6%) and the lowest TPC was found in seed extract (4.67%). The antioxidant activity test presented the value of IC50 of the methanol extract of root, seed, stem, and leaf as 129.65 µg/ml, 255.04 µg/ml, 145.28 µg/ml, and 112.96 µg/ml, respectively. Furthermore, the stem showed antibacterial activity by inhibiting the growth of B. subtilis and S. aureus in a concentration of 10% of the sample extract (v/v). Leaf extract has activity against only S. aureus growth in different concentrations (2.5, 5, 10%), while all bacteria were resistant to root and seed extracts. The stem extract showed the highest antioxidant and antibacterial activity compared to other parts.
Co-Authors Anita Apriliani Dwi Rahayu Assyafiya Salwa Ganis Lukmandaru Krisnawati Nastiti Wijayanti Rizki Arisandi Tri Rini Nuringtyas