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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika
Muhaimin Rifa'i
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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Journal : Biotropika

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A Comparative Profile of Free Radicals, Endogenous Antioxidants, and Cytokines in Mouse Model of Type 1 Diabetes Mellitus Fikriya Novita Sari; Rizky Senna Samoedra; Setyaki Kevin Pratama; Sri Rahayu; Aris Soewondo; Muhaimin Rifa'i
Biotropika: Journal of Tropical Biology Vol. 11 No. 3 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2023.011.03.04

Abstract

Diabetes mellitus is a metabolic disorder characterized by high blood glucose (hyperglycemia). Hyperglycemia will cause the body to undergo physiological changes such as free radical, antioxidant and inflammation alteration. This research aims to compare the profile of free radicals, endogenous antioxidants, and cytokines in mouse models of type 1 diabetes mellitus (T1DM). Mice were separated into two different groups, normal and diabetic mice groups. The normal group was a group of mice that were not induced to have diabetic conditions, while the diabetic mice group was induced to be diabetic using streptozotocin injection. Blood glucose levels were checked every three days for 14 days, while the immune response was evaluated after 14 days using flow cytometry. Data analysis was done using SPSS software with t-test analysis. This research showed that the increasing ROS represented by MDA would trigger inflammation in T1DM represented by the increasing TNF-alpha along with IFN-gamma and reducing anti-inflammatory cytokines represented by IL-10. Interestingly, SOD expression, which is an endogenous antioxidant, is also increased in the diabetic mice group, and we conclude that it is some sort of adaptive response of the diabetic mice group against the increasing ROS.
Epigenetic Profiling of Tumor Suppressor Genes Methylation in Breast Cancer Using the MS-MLPA Method Zhafira, Alifya; Adhitya Bayu Perdana; Botista Agung Maryanto; Muhaimin Rifa'i
Biotropika: Journal of Tropical Biology Vol. 14 No. 1 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2026.014.01.01

Abstract

Breast cancer remains the leading cause of cancer-related mortality among women globally, with a high incidence rate reported in Indonesia. Early detection is essential for improving the prognosis and therapeutic outcomes. DNA methylation of tumour suppressor genes (TSGs) has emerged as a promising biomarker for cancer diagnosis. This study aimed to identify the methylation status of TSGs in breast cancer tissues using the Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA) method. A total of 44 breast cancer tissue samples and three normal tissues (negative controls) were obtained from the Biobank of Dharmais Cancer Hospital. DNA was extracted and analysed using the ME001-D1 probemix (MRC-Holland) according to the manufacturer’s instructions. Methylation status was evaluated using Coffalyser.Net. of the 44 samples analysed, 20 (45.4%) exhibited promoter methylation in one or more TSGs, whereas 24 samples (54.6%) showed no methylation. Methylation was identified in nine genes: RASSF1, CDKN2A, APC, CDH13, GSTP1, DAPK, CADM1, BRCA1, and TIMP3. RASSF1 showed the highest frequency (75%), followed by CDKN2A (40%) and APC (30%). Genes such as CDH13, GSTP1, and others appeared at lower frequencies. These findings confirm that aberrant DNA methylation of TSGs plays a critical role in breast cancer pathogenesis. Further studies are recommended to validate the diagnostic value of these genes and explore their potential clinical applications in breast cancer management.
Co-Authors Adhitya Bayu Perdana Aris Soewondo Botista Agung Maryanto Fikriya Novita Sari Moch Sasmito Djati Rizky Senna Samoedra Setyaki Kevin Pratama Shofiyah, Aya SRI RAHAYU Young-Ho Lee Zhafira, Alifya