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Nur Afia Ameliani
Universitas Sulawesi Barat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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AKTIVITAS ANTIBAKTERI EKSTRAK DAUN AKASIA (Acacia auriculiformis) TERHADAP PERTUMBUHAN BAKTERI Bacillus sp., Staphylococcus aureus DAN Escherichia coli Nurul Hidayah; Gaby Maulida Nurdin; Nur Afia Ameliani
bionature Vol 23, No 2 (2022): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v23i2.37593

Abstract

Meningkatnya kejadian resistensi bakteri terhadap antibiotik, mendorong pemanfaatan obat herbal dalam mengobati penyakit infeksi. Daun Acacia auriculiformis diketahui memiliki senyawa metabolit yang dapat menghambat pertumbuhan bakteri Gram positif dan negatif. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun A. auriculiformis terhadap pertumbuhan bakteri Bacilus sp., Staphylococcus aureus dan Escherichia coli secara in vitro. Penelitian ini dilakukan dengan mengekstrak daun A. auriculiformis menggunakan pelarut etanol. Uji daya hambat ekstrak daun A. auriculiformis dilakukan dengan metode difusi sumur. Adanya aktivitas antibakteri ditunjukkan dengan adanya zona bening di sekitar sumur. Hasil penelitian menunjukkan bahwa ekstrak daun A. auriculiformis memiliki kemampuan menghambat pertumbuhan bakteri Bacillus sp. dan Staphylococcus aureus dengan kategori daya hambat tergolong kuat. Ekstrak daun A. auriculiformis tidak mampu menghambat pertumbuhan bakteri Escherichia coli. Uji golongan senyawa dengan metode skrining fitokimia menunjukkan bahwa daun akasia mengandung senyawa flavonoid dan tanin yang bersifat antibakteri.
Aktivitas Antibakteri Ekstrak Daun Akasia (Acacia auriculiformis) terhadap Pertumbuhan Bakteri Bacillus sp., Staphylococcus aureus dan Escherichia coli Nurul Hidayah; Gaby Maulida Nurdin; Nur Afia Ameliani
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The increasing of bacterial resistance to antibiotics, which encourages the use of herbal medicines in treating infectious diseases. Acacia auriculiformis leaves are known to have metabolites that can act as an antibacterial agent. This study aimed to determine the antibacterial activity of A. auriculiformis leaves extract against the growth of Bacillus sp., Staphylococcus aureus and Escherichia coli bacteria in vitro. This research was conducted by extracting the leaves of A. auriculiformis using ethanol as a solvent. The inhibition test of A. auriculiformis leaf extract was carried out using the well diffusion method. Antibacterial activity was indicated by the presence of a clear zone around the well. The results showed that the leaf extract of A. auriculiformis had the ability to inhibit the growth of Bacillus sp. and Staphylococcus aureus with the category of strong inhibition. Leaf extract of A. auriculiformis was not able to inhibit the growth of Escherichia coli. Phytochemicals screening test showed that A. auriculiformis leaf contains flavonoids and tannins compounds as antibacterial. Therefore, A. auriculiformis leaves extract had the ability to inhibit the growth of Gram positive bacteria but not Gram negative bacteria.
Co-Authors Nurdin, Gaby Maulida Nurul Hidayah