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Journal : Warta Rimba

ORGANOGENESIS TANAMAN GAHARU (Aquilaria malaccensis Lamk) PADA BERBAGAI KONSENTRASI ZAT PENGATUR TUMBUH Benzyl Amino Purin (BAP) - Indole Butiric Acid (IBA) SECARA IN-VITRO A, Arhvitasari; m, Muslimin; w, Waeniyanti; w, wardah
Jurnal Warta Rimba Vol 7, No 3 (2019)
Publisher : Universitas Tadulako

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Abstract

Agarwood (Aquilaria malaccensis Lamk) is one type of forestry plant that has been developed with tissue culture techniques. The provision of growth regulators can be done by adding in the growing media used. Growth regulators used are usually in the form of auxins and cytokines. This study aims to obtain the concentration of growth regulators with the combination of Benzyl Amino Purine (BAP) -Indole Butyric Acid (IBA) which is the best for organogenesis of aloe plants (Aquilaria Malaccensis Lamk) in vitro. This study uses a completely randomized design (CRD) method consisting of 5 BAP-IBA combination treatments namely V0 = MS0 (control), V1 = MS + 0.8ppm BAP + 0.1ppm IBA, V2 = MS + 1.0ppm BAP + 0 , 1ppm IBA, V3 = MS + 1.5ppm BAP + 0.1ppm IBA, V4 = MS + 2.0ppm BAP + 0.1ppm IBA. The parameters observed in this study were the initial emergence of shoots, the number of shoots, and a number of leaves. The Smallest Significant Difference (BNT) test was carried out if the analysis of variance showed that the BAP-IBA combination treatment had a significant effect. The results showed that the combination of BAP-IBA on various treatments that were tried had a very significant effect on the initial emergence of shoots, a number of shoots and a number of leaves. The best treatment was found in V1 with MS concentration + 0.8ppm BAP + 0.1ppm IBA was able to induce early formation of the fastest buds with an average of 5.00 days after planting (HST), the highest number of shoots with an average of 3.00 shots, the highest average number of leaves is 1.80 strands, and the highest growth percentage is 100.00%.
PENGARUH JENIS BAHAN ORGANIK DAN KONSENTRASI Azotobacter sp TERHADAP PERTUMBUHAN SEMAI JATI (Tectona grandis Linn. F) s, Syaifuddin; m, Muslimin
Jurnal Warta Rimba Vol 7, No 2 (2019)
Publisher : Universitas Tadulako

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Abstract

Azotobacter is a non-symbiotic bacterium and can capture N in the air. Azotobacter lives as heterotrophic bacteria that need organic as food. Azotobacter has the potential to developed in Teak plants, considering that Teak is not from legume. This study aims to types of organic and the concentration Azotobacter sp on the growth of Teak plants (Tectona grandis Linn. F). The study conducted three months from January to March 2019, which conducted at the Temporary Nursery Area of the Faculty of Forestry, Tadulako University. This study uses a Completely Randomized Design (CRD) factorial pattern with two factors consisting of organic matter factors, without organic matter (B0), straw (B1), goat manure (B2), and cocoa skin (B3), the second factor, Concentration Azotobacter sp 0 ml+10 liters waters (M0), 50ml+10 liters waters (M1), 100ml+10 liters waters (M2), 150ml+10 liters waters (M3), and 200ml+10 liters waters (M4). The results showed cocoa skin (B3) had an average height increase of 12.52 cm and an increase the number leaves of 8.07 strands, significantly different from treatment without organic matter (B0). The interaction of Azotobacter sp 200ml+10 liters waters (M4) concentration has an average height increase of 13.62 cm and the addition of leaves of 7.92 strands is significantly different from the consentration of Azotobacter sp 0ml+10 liters waters (M0). Azotobacter sp 200ml+10 liters waters (B3M4) gave the highest increase in average of 16.23 cm, number of leaves 9.33 strands, and 0.43 cm in diameter differed Azotobacter sp 0ml+10 liters waters (B0M0).Key Words : Teak, Organik Matter, Azotobacter