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STUDI VARIABILITAS GENETIK 30 GENOTIPE PADI (ORYZA SATIVA L.) MENGGUNAKAN BEBERAPA MARKA SSR (SIMPLE SEQUENCE REPEAT) TERPAUT ZN Tiwi Rumondang Pangaribuan; Muhammad Syafi'i; Elia Azizah; Untung Susanto; Furry Pramudyawardani; Desi Prastika
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 7, No 4 (2022): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v7i4.7479

Abstract

Padi merupakan salah satu komoditas penting di Indonesia. analisis keragaman genetik padi diperlukan untuk keberhasilan program pemuliaan  varietas padi lokal. Penelitian ini bertujuan untuk mengidentifikasi variabilitas genetik dan pola kekerabatan tiga puluh genotipe padi (Oryza sativa L) menggunakan beberapa marka SSR terpaut Zink. Penelitian dilaksanakan pada bulan September - Desember 2021 di Laboratorium DNA  Balai Besar Penelitian Tanaman Padi, Sukamandi. Sebanyak 30 genotipe padi lokal dengan kandungan Zn yang beragam telah dianalisis menggunakan eksperimen laboratorium. Hasil penelitian menunjukkan bahwa terdapat variasi alel yang berbeda  (2 - 8) di antara genotipe-genotipe yang di uji dengan rerata jumlah alel 4,5, sedangkan rerata nilai Polymorphism Information Content (PIC) sebesar 0,48  (0,20 - 0,70). Terdapat 4 marka SSR yang memiliki nilai PIC > 0,5 yaitu RM162, RM38, RM30 dan RM80 yang menunjukkan bahwa marka-marka tersebut informatif untuk studi keragaman genetik padi dengan ragam kandungan Zn dengan rerata nilai diversitas gen sebesar 0,53. Hasil analisis filogenetik menunjukkan bahwa 30 genotipe tersebut mengelompok menjadi lima kelompok dengan koefisien kemiripan 0,68.
Uji Daya Hasil Beberapa Galur Jagung Lapang Padjadjaran (Zea mays L.) di Dataran Medium Kabupaten Sumedang Maslatul Umah; Muhammad Syafi'i; Nurcahyo Widyodaru Saputro; Dedi Ruswandi
JURNAL AGROPLASMA Vol 13, No 2 (2026): Jurnal Agroplasma Vol.13 No.2 (Oktober 2026)
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v13i2.9484

Abstract

Corn (Zea mays L.) is a superior agricultural commodity that has a strategic role in the food and livestock sectors because it is used as a food source and raw material for animal feed. One effort to increase corn productivity is through plant breeding techniques. Yield testing is carried out to see the potential yield of candidate varieties and identify superior lines. This study aims to identify the Padjadjaran field corn line (Zea mays L.) that has a higher yield potential than the comparison varieties based on the parameter characters of the weight of the cob with husk, weight of the cob without husk, length of the cob with husk, length of the cob without husk, diameter with husk, diameter without husk, number of rows, and weight of 100 seeds. This study was conducted in an experimental field owned by the Faculty of Agriculture, Padjadjaran University located in Kutamandiri Village, Tanjungsari District, Sumedang Regency. The entire series of research activities were carried out in March–July 2025. The research method used was Augmented Design consisting of 60 Padjadjaran field corn lines without repetition and 5 commercial varieties as controls (check) repeated 3 times arranged in 3 experimental blocks. Data analysis used the F test at 5% level and the LSD (Least Significant Difference) advanced test at 5%. The results showed that GJP37 produced the highest cob weight with husk weighing 313.50 grams, the highest cob weight without husk weighing 268.00 grams and the highest yield (tons/ha) of 10.76 tons/ha. GJP42 produced the highest cob length with husk of 34.98 cm and the highest cob length without husk was achieved by GJP53 22.76 cm. GJP40 produced the highest corn cob diameter with husks, at 55.76 mm, and GJP29 produced the highest corn cob diameter without husks, at 52.35 mm. GJP41 produced the highest 100-seed weight, at 36.47 grams. GJP13 produced the highest number of rows per ear, at 16.67 rows.Keywords: Corn Lines, Productivity, Plant Breeding, Yield, Augmented Design