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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL KIMIA SAINS DAN APLIKASI Journal of Biomedicine and Translational Research Journal of Science and Applicative Technology Jurnal Kreativitas PKM Solo Journal of Anesthesi, Pain and Critical Care Medical Sains : Jurnal Ilmiah Kefarmasian Medical Sains : Jurnal Ilmiah Kefarmasian
Khairul Anam
Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Engineering Environmental Science Health Professions Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health

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Structural Stability of ADTC5 Peptide: Conformational Insights into Dynamics and Its Binding Mode Parsaoran Siahaan; Vivitri Dewi Prasasty; Bungaran David Simanjuntak; Suci Hildayani; Khairul Anam
Journal of Tropical Life Science Vol. 7 No. 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.09

Abstract

The cyclic structure of ADTC5 (Ac-CDTPPVC-NH2) peptide is known has ability to modulate homo dimer E-cadherin interactions to form adherens junction at the intercellular junction.  The ability to inhibit E-cadherin interaction has become important to increase paracellular porosity in delivering drug molecules to the target cell. There are two types of ADTC5 state: opened-cyclic state (OCS) and closed-cyclic state (CCS). OCS of ADTC5 is affected by distance constraint and CCS is formed by disulfide bond from terminal cysteines through force restraint. The aim of this research is to investigate the inhibition activity of ADTC5 peptide upon E-cadherin. Here we used molecular docking and molecular dynamics approaches. The structure of ADTC5 peptide was generated by PyMOL program. GROMACS v4.5.5 was utilized to simulate molecular dynamics. The optimized ADTC5 molecule was placed in aqueous or polar condition at physiological pH. Furthermore, ADTC5 was docked with EC1-EC2 coupled domain of E-cadherin using AutoDock 4.2 and be refined using molecular dynamic (MD). The result showed that CCS ADTC5 peptide has stronger affinity and more stable interaction with EC1-EC2 coupled domain than the OCS one.
IDENTIFIKASI KUMARIN DAN PENGARUH JENIS PELARUT TERHADAP TOTAL KUMARIN PADA EKSTRAK BUAH API-API PUTIH (A. marina): COUMARIN IDENTIFICATION AND EFFECT OF SOLVENTS ON TOTAL COUMARIN CONTENT OF API-API PUTIH FRUIT (A. marina) EXTRACT Alik Kandhita Febriani; Khairul Anam
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 7 No 4 (2022)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v7i4.452

Abstract

Api-api putih (Avicennia marina) adalah salah satu jenis mangrove yang memiliki kandungan senyawa flavonoid, alkaloid, tannin, steroid, saponin, terpen, glikosida, dan kumarin. Pada tanaman api-api putih, kadar kumarin total tertinggi ada pada buahnya dibandingkan dengan daun dan kulit batangnya. Penelitian ini dilakukan untuk mengetahui kadar kumarin total ekstrak buah api-api putih yang diekstraksi dengan pelarut yang berbeda serta profil senyawa kumarin pada tiap pelarut menggunakan metode KLT. Ekstraksi dilakukan dengan metode sokletasi secara sekuensial dengan pelarut berturut-turut n-heksana, kloroform, metanol, dan air. Penentuan kadar kumarin total diukur menggunakan spektrofotometer UV-Vis pada panjang gelombang 274 nm. Total kumarin dinyatakan dalam mg CE (Coumarin Equivalent)/100 g ekstrak. Kumarin (1,2 benzopyrone) digunakan sebagai senyawa standar. Skrining fitokimia menunjukkan simplisia buah api-api putih mengandung alkaloid, tannin, saponin, flavonoid, dan steroid/triterpenoid. Ekstrak metanol merupakan ekstrak dengan rendemen terbanyak yaitu 22,22% diikuti dengan ekstrak air (12,77%), kloroform (0,48%), dan n-heksana (0,36%). Kadar kumarin total tertinggi yaitu 80,9 ± 1,4 mg CE/100 g ekstrak metanol diikuti dengan ekstrak air sejumlah 17,5 ± 1,4 mg CE/100 g ekstrak, ekstrak kloroform 10,9 ± 0,4 mg CE/100 g ekstrak, dan ekstrak n-heksan sejumlah 6,08 ± 0,4 mg CE/100 g ekstrak. Hasil penentuan kumarin total dapat dilihat bahwa kadar kumarin total tertinggi ada pada ekstrak metanol didukung dengan hasil identifikasi menggunakan metode KLT yang memperlihatkan noda kumarin yang terlihat jelas dibandingkan dengan ekstrak lainnya. Hal ini menunjukkan bahwa metanol adalah pelarut terbaik untuk mengekstrak kumarin pada buah api-api putih.
Identification of Alkaloid Compounds from Cytotoxic Active Fraction in Peperomia pellucida M Alvien Ghifari; Enny Fachriyah; Khairul Anam; Dian Nopitasari; Kautsar Elvira; Rama Esa Perdana
Journal of Science and Applicative Technology Vol 5 No 1 (2021): Journal of Science and Applicative Technology June Chapter
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/jsat.v5i1.394

Abstract

Peperomia pellucida attracts a lot of interest due to its bioactivity and has been used in various folk medicine. In this report, n-hexane, dichloromethane, and ethyl acetate fraction of Peperomia pellucida extract were examined for its cytotoxic activity against Artemia salina larvae using the BSLT method. The ethyl acetate fraction showed higher cytotoxic activity than n-hexane and dichloromethane. The LC50 of ethyl acetate, n-hexane, and dichloromethane fractions was 39.665, 278.920, and 60.808 ppm, respectively. Mayer and Dragendorff tests give a positive result to the alkaloid constituent in ethyl acetate fraction. The alkaloids in Peperomia pellucida were extracted using ethyl acetate and further separated using chromatography techniques. Two alkaloid components were identified in ethyl acetate fraction from Peperomia pellucida as Piperine and Nigramide N by using LC-MS/MS.
Isolation and Antioxidant Activity of Flavonoid Compound in Ethanolic Extract of Celery Leaves (Apium graveolens L.) Alifa Husnun Kholieqoh; Khairul Anam; Dewi Kusrini
Jurnal Kimia Sains dan Aplikasi Vol 25, No 12 (2022): Volume 25 Issue 12 Year 2022
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.25.12.450-455

Abstract

Celery (Apium graveolens L.) is a plant that belongs to the Apiaceae family and is widely used as a medical plant for low blood pressure, heart tonic, and to prevent cardiovascular disease. This study aims to obtain flavonoid compounds, identify, and test the antioxidant activity of flavonoid compounds and crude extracts from celery leaves. The research procedures consisted of four steps, the first of which was a preliminary test. The second step involved isolating and separating flavonoid components by vacuum liquid chromatography, gravitational column chromatography, and preparative thin layer chromatography. The third step was to identify flavonoid compounds using reagent shift, FTIR, and LCMS/MS. And finally, antioxidant activity was evaluated using the DPPH method. The preliminary test result showed that the ethanolic extract of leaves and stems had a total flavonoid content of 13.99 and 2.46 mg QE/g of dry weight. Both dry leaves and crude extract of celery leaves contained alkaloids, saponin, flavonoid, tannin, quinone, and steroid/triterpenoid, as determined by phytochemical screening. Isolation and separation of flavonoids yielded A2.I and A2.II isolates, with respective weights of 8 mg and 14 mg. Identification of flavonoid compounds using reagent shift showed that two isolates have the basic structure of the flavone group. A2.I isolate had OH groups at 4’, 5, and 7, while A2.II isolate had OH groups at 3’, 4’, 5, and 7. The FTIR analysis revealed that both compounds contain functional groups, including O-H, C=O, C=C aromatic, C-O ether, C-O alcohol, and C-H aromatic ring. According to LCMS/MS analysis, the molecular weights of A2.I and A2.II were 270 g/mol and 286 g/mol, respectively. All of the identification methods for isolates showed that A2.I was apigenin and A2.II was luteolin. Antioxidant activity by DPPH method for a viscous extract of celery leaves, A2.I, and A2.II were 775.41, 288.95, and 184.35 µg/mL, respectively.
The Activity of Liposome-Parijoto Formula Through p53 Expression in HepG2 Cell Line widyandani sasikirana; Ragil Setia Dianingati; Khairul Anam; eva annisaa'; evieta Rohana
Journal of Biomedicine and Translational Research Vol 9, No 2 (2023): August 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i2.17290

Abstract

Parijoto, one of Melastomaceae family, has been known to have cytotoxic activity in HepG2, a hepatocellular cancer cell line, but with low activity. However, the ethyl acetate fraction of Parijoto gave the highest antioxidant and cytotoxic activity in 4T1. Then, purification and liposome formulation need to be carried out to increase the activity of Parijoto extract.  Objective: This research aimed to study the cytotoxic activity and molecular mechanism of LEA (Liposom-Ethyl Acetate of Parijoto Fraction) in HepG2. Method: Extraction has been done by maceration, followed by partition using n-hexane, ethyl acetate, and methanol. LEA formulation was carried out by thin-layer hydration with modification and the formula was sized using a bath sonicator.  Cytotoxic activity test of LEA and extract was performed in five serial concentrations (3,9 µg/mL–250 µg/mL), while the positive control doxorubicin performed in 3,9 µg/mL – 250 µg/mL by MTT assay. P53 gene expression was analyzed by using PCR-electrophoresis. Result: Results showed that LEA increased the cytotoxic activity (IC50 = 28.40 μg/ml). Furthermore, based on the electrophoresis study, LEA induced the p53 expression while the extract only did not. Conclusion: Liposome formula from ethyl acetate fraction of Parijoto extract (LEA) was able to increase cytotoxic activity and p53 gene expression was possible through the apoptotic mechanism. This shows that this formula is a promising strategy to improve the bioavailability of herbal medicines as cytotoxic agents.
IDENTIFIKASI KUMARIN DAN PENGARUH JENIS PELARUT TERHADAP TOTAL KUMARIN PADA EKSTRAK BUAH API-API PUTIH (A. marina): COUMARIN IDENTIFICATION AND EFFECT OF SOLVENTS ON TOTAL COUMARIN CONTENT OF API-API PUTIH FRUIT (A. marina) EXTRACT Alik Kandhita Febriani; Khairul Anam
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 7 No 4 (2022)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v7i4.452

Abstract

Api-api putih (Avicennia marina) adalah salah satu jenis mangrove yang memiliki kandungan senyawa flavonoid, alkaloid, tannin, steroid, saponin, terpen, glikosida, dan kumarin. Pada tanaman api-api putih, kadar kumarin total tertinggi ada pada buahnya dibandingkan dengan daun dan kulit batangnya. Penelitian ini dilakukan untuk mengetahui kadar kumarin total ekstrak buah api-api putih yang diekstraksi dengan pelarut yang berbeda serta profil senyawa kumarin pada tiap pelarut menggunakan metode KLT. Ekstraksi dilakukan dengan metode sokletasi secara sekuensial dengan pelarut berturut-turut n-heksana, kloroform, metanol, dan air. Penentuan kadar kumarin total diukur menggunakan spektrofotometer UV-Vis pada panjang gelombang 274 nm. Total kumarin dinyatakan dalam mg CE (Coumarin Equivalent)/100 g ekstrak. Kumarin (1,2 benzopyrone) digunakan sebagai senyawa standar. Skrining fitokimia menunjukkan simplisia buah api-api putih mengandung alkaloid, tannin, saponin, flavonoid, dan steroid/triterpenoid. Ekstrak metanol merupakan ekstrak dengan rendemen terbanyak yaitu 22,22% diikuti dengan ekstrak air (12,77%), kloroform (0,48%), dan n-heksana (0,36%). Kadar kumarin total tertinggi yaitu 80,9 ± 1,4 mg CE/100 g ekstrak metanol diikuti dengan ekstrak air sejumlah 17,5 ± 1,4 mg CE/100 g ekstrak, ekstrak kloroform 10,9 ± 0,4 mg CE/100 g ekstrak, dan ekstrak n-heksan sejumlah 6,08 ± 0,4 mg CE/100 g ekstrak. Hasil penentuan kumarin total dapat dilihat bahwa kadar kumarin total tertinggi ada pada ekstrak metanol didukung dengan hasil identifikasi menggunakan metode KLT yang memperlihatkan noda kumarin yang terlihat jelas dibandingkan dengan ekstrak lainnya. Hal ini menunjukkan bahwa metanol adalah pelarut terbaik untuk mengekstrak kumarin pada buah api-api putih.
Inovasi Produk Jamu dan Strategi Komunikasi Visual: Pendampingan Kemandirian Ekonomi Santri Pondok Pesantren Yayasan Ar Rodiah Semarang Wimzy Rizqy Prabhata; Evieta Rohana; Khairul Anam; Fitri Wulandari; Widyaningrum Utami; Eva Annisaa; Intan Rahmania Eka Dini; Adhe Setya Pramayoga
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 9, No 5 (2026): Volume 9 Nomor 5 (2026)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v9i5.25431

Abstract

ABSTRAK Jamu merupakan warisan pengobatan herbal Nusantara yang memiliki potensi ekonomi besar namun sering kali tidak menarik minat generasi muda. Diperlukan inovasi produk dan strategi visual yang kuat agar jamu dapat bersaing di pasar saat ini. Yayasan Ar Rodiah Semarang memiliki santri usia remaja dengan potensi kreativitas tinggi yang dapat diarahkan menjadi produsen kreatif melalui pendampingan kewirausahaan. Kegiatan ini bertujuan untuk meningkatkan pengetahuan santri mengenai penyakit degeneratif, mengajarkan pembuatan jamu dalam bentuk herbal shot, dan meningkatkan kemampuan desain visual dan pemasaran. Kegiatan pengabdian ini dilaksanakan oleh tim KKNT 118 Universitas Diponegoro pada Juni hingga Agustus 2025 dengan melibatkan 29 santri. Metode yang digunakan adalah pendampingan partisipatif yang meliputi edukasi kesehatan, praktik formulasi sediaan, pelatihan teknik fotografi dan videografi produk, hingga uji coba pasar di Car Free Day (CFD) Tembalang. Data dikumpulkan melalui instrumen pre- dan post-test untuk mengukur peningkatan pengetahuan dan keterampilan. Hasil penilaian mendemonstrasikan peningkatan signifikan pada pengetahuan dan keterampilan santri. Skor rata-rata meningkat sebesar 35,89 poin, dari nilai awal (pre-test) 52,31 menjadi 88,21 pada post-test. Santri berhasil memproduksi herbal shot yang siap untuk dipasarkan dan mampu menyusun konten pemasaran visual yang estetik untuk meningkatkan daya tarik produk. Pendampingan interaktif dan terintegrasi dari hulu hingga hilir terbukti efektif mengubah paradigma santri dari sekadar penjual jamu tradisional menjadi produsen kreatif. Integrasi antara inovasi produk herbal dan komunikasi visual menjadi kunci utama dalam meningkatkan daya saing produk jamu di pasar modern. Kata Kunci: Herbal Shot, Jamu, Komunikasi Visual, Kreativitas Remaja, Pendampingan Partisipatif                                               ABSTRACT Jamu, an Indonesian herbal medicine, has significant economic potential but often fails to attract younger generations. Strong product innovation and visual strategies are essential for jamu to compete in today's market. The Ar Rodiah Foundation in Semarang oversees teenage students (santri) with high creative potential who can be directed toward becoming creative producers through entrepreneurial mentoring. This activity aims to increase students' knowledge regarding degenerative diseases, the production of jamu in the form of "herbal shots," and the enhancement of visual design and marketing capabilities. The UNDIP KKNT 118 team conducted this community service activity from June to August 2025, involving 29 students. The method used was participatory mentoring, which included health education, formulation practice, training in product photography and videography techniques, and market testing at the Tembalang Car Free Day (CFD). Data were collected through pre- and post-tests to measure improvements in knowledge and skills. The results showed a significant increase in students' knowledge and skills. The average score increased by 35.89 points, rising from an initial pre-test value of 52.31 to 88.21 in the post-test. The students successfully produced herbal shots tailored to market preferences and were able to create aesthetically pleasing visual marketing content to enhance product appeal. Interactive and integrated mentoring, from upstream to downstream, has proven effective in shifting students' paradigms from traditional jamu sellers to creative producers. The integration of herbal product innovation and visual communication is the primary key to increasing the competitiveness of jamu products in the modern market. Keywords: Herbal Shot, Jamu, Visual Communication, Adolescent Creativity, Participatory Mentoring.
Chemical Composition, Physicochemical, and Pharmacokinetics Profile of Custard Apple Leaf (Annona squamosa) Essential Oil Extract Khrisna Pangeran; Khairul Anam; Dewi Kusrini
Jurnal Kimia Sains dan Aplikasi Vol 28, No 8 (2025): Volume 28 Issue 8 Year 2025
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.28.8.442-451

Abstract

This study investigates the effects of different drying methods on the chemical composition, physicochemical characteristics, and pharmacokinetic properties of custard apple (Annona squamosa) leaf essential oils. The research also evaluates how these drying techniques influence the yield and quality of the oils, with particular emphasis on terpenoid content and their pharmacological relevance. Fresh custard apple leaves were subjected to four drying methods: shade drying, sun drying, oven drying at 45°C, and air conditioning (AC) drying at 20°C. Essential oils were extracted through steam distillation and analyzed using GC–MS to determine their chemical constituents. Various physicochemical parameters, including refractive index, specific gravity, acid value, and saponification value, were measured. Pharmacokinetic properties were assessed using the pkCSM online tool, while network visualization was performed via STRING.db. The drying methods significantly affected the essential oil yield, with oven drying producing the highest yield. Based on physicochemical analysis, shade drying resulted in the highest acid and ester values. The chemical profiles varied across treatments, with trans-β-caryophyllene, germacrene D, and α-humulene identified as the dominant compounds in all samples. Network pharmacology analysis indicated that CYP2C9 and CYP2C19 play critical roles in optimizing personalized therapy by minimizing adverse effects and enhancing drug efficacy across various diseases.
A PECS II Block as Post Operative Analgesia After Modified Radical Mastectomy Imron - Rosyadi; Khairul Anam; Mochamat Mochamat
Solo Journal of Anesthesi, Pain and Critical Care (SOJA) Vol 4, No 2 (2024): October 2024
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/soja.v4i2.67195

Abstract

Background: In Indonesia, breast cancer is the most common cancers in 2018, with a mortality rate of 11%. Postoperative pain is a complication that can occur after MRM surgery. The implementation of Enhanced Recovery After Surgery (ERAS) is currently increasingly being applied to breast surgery, one of which is by reducing the use of opioids. Selection of regional anesthetic technique as an alternative used in the form of neuraxial and peripheral nerve blocks. The use of regional anesthetic techniques also reduces the side effects of opioids that are often found, namely Post Operative Nausea and Vomiting (PONV) and pruritus. Analgesic technique with pectoral nerve block (PECS block) is an effective alternative postoperative MRM pain treatment in addition to opioid analgesia.Methods: An analytical experimental study with double-blind randomized controlled trial (RCT) design. The study population was all patients who underwent MRM surgery at Dr. Kariadi General Hospital in May 2021 - August 2021. The study sample consisted of 46 subjects that obtained using the random allocation method, and randomly divided into two groups (PECS II block and control group). Postoperative pain scores, time to first request of rescue analgesic, total dose of rescue analgesic, incidence of PONV, and pruritus within 24 hours postoperative were analyzed in both groups.Results: Based on the results of the analysis, the mean pain scores at 4, 12 and 24 hours postoperative were significantly lower in the PECS II block group compared to the control group (p=0.001, p=0.013, and p=0.003). The mean time to first use of rescue analgesics was longer and the total dose of rescue analgesics was lower in the PECS II block group (p=0.00, p=0.00). The incidence of PONV ((69,5 %) and postoperative pruritus (17,4 %) were more common in the control group (p=0.001, p=0.346)Conclusion: PECS II block is effective as postoperative analgesic after modified radical mastectomy.
Co-Authors Adhe Setya Pramayoga Alifa Husnun Kholieqoh Alik Kandhita Febriani Bungaran David Simanjuntak Dewi Kusrini Dewi Kusrini Dian Nopitasari Enny Fachriyah Eva Annisaa eva annisaa' Evieta Rohana evieta Rohana Fitri Wulandari Ghifari, M Alvien Imron - Rosyadi Intan Rahmania Eka Dini Kautsar Elvira Khrisna Pangeran Mochamat Mochamat Parsaoran Siahaan Ragil Setia Dianingati Rama Esa Perdana Suci Hildayani Vivitri Dewi Prasasty widyandani sasikirana Widyaningrum Utami Wimzy Rizqy Prabhata