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Kloning Gen pcbC dari Penicillium chrysogenum ke dalam Plasmid pPICZA untuk Pengembangan Produksi Penisilin G Wiharyani, Risma; Hardianto, Dudi; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 16, No.1, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Availability of drugs in Indonesia is still limited by the high prices of drugs due to on the imported raw materials that reaches 95%. Developing antibiotic raw materials can be achieved by increasing of penicillin G production, which is the raw material for the formation of semisynthetic penicillin derivatives through the production of 6-aminopenisillanic acid (6-APA). One of the important enzyme in the penicillin G biosynthesis is Isopenisilin N Synthase (IPNS) that encodes by pcbC gene on Penicillium chrysogenum. This study aimed to obtain a recombinant of pcbC gene fragments that is inserted into pPICZA plasmid. Amplification of pcbC gene used pcbC-F and pcbC-R primers. The pcbC gene fragment was inserted into pPICZA vector and then transformed into TOP 10 Fâ€™. The results showed that the recombinant of the pcbC gene fragment from P. chrysogenum has been obtained. Analysis of DNA sequences using the BLAST program showed that the pcbC gene fragment has high homology (99%) with the Â pcbC gene from P. chrysogenum Wisconsin 54-1255 and P. chrysogenum AS-P-78 which encodes IPNS Â Keywords: pcbC Gene, Penicillium chrysogenum, cloning, penicillin G

HUBUNGAN FAKTOR DEMOGRAFI DENGAN MOTIVASI MENJADI GURU SEBAGAI PILIHAN KARIR : SELF-REPORTED SURVEY Ahmad Kamal Sudrajat; Risma Wiharyanti; Fera Aulia; Dita Purwinda Anggrella
JURNAL TARBIYAH Vol 29, No 2 (2022)
Publisher : UIN Sumatera Utara Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30829/tar.v29i2.1863

Motivation to become a teacher is important information to be used in preservice teacher education programs. The purpose of this study was to determine the relationship between demographic factors and motivation to choose teachers as career choices in teacher education students. The type of research used in this research is survey. The research subjects were students of the second year at the Department of Biology Education, Universitas Negeri Yogyakarta. The results showed that hometown of students had a relationship with motivation to become a teacher, students from city areas had higher scores. The results of the analysis on each aspect indicate that hometown of students has a relationship with the aspect of job security (Sig = 0.036) and gender has a relationship with the aspect of work with children and adolescents (Sig = 0.042). In addition, gender and hometown of students have a relationship with aspects of enhancing social equality (Sig = 0.015; Sig = 0.021). The conclusion in this study is that the student's hometown has a relationship with their motivation to become a teacher as a career choice, while gender and family background have no relationship.

16S Metagenomics Of Branch-Leaf Compost At The Expo Memorial Park Osaka: 16S Metagenomik Kompos Cabang-Daun Di The Expo Memorial Park Osaka Fera Aulia; Risma Wiharyanti
Journal of Multidisciplinary Science Vol. 2 No. 2 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Institut Studi Islam Sunan Doe

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58330/prevenire.v2i2.187

Compost is one of the potential microbial habitats for the discovery of a wide range of novel biocatalysts. Compost sample originally made of plant litter, were collected at the Expo Park (Osaka, Japan). The temperature of composts is around 50 °C. Metagenomic DNA was isolated from compost and was used to amplify partial 16S rRNA genes (rDNAs) by polymerase chain reaction for investigation of the microbial diversity in the compost. Molecular microbial identification was carried out by metagenome analysis of 16S rRNA using MinION Next Generation Sequencing (NGS). The results indicated that branch-leaf compost sample from Expo Park is dominated by thermophilic microorganism. The majority of bacteria are Caldicoprobacter, Hungateiclostridium, and Thermoclostridium. To conclude, thermophilic microorganism is not only found in extreme environment, they also presence even in moderate temperature.

In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA

Efektivitas PGPR dan Nanosilika terhadap Pertumbuhan dan Hasil Kedelai Varietas Anjasmoro di Tanah Regosol Umami, Arif; Wiharyanti, Risma
Jurnal Agro Wiralodra Vol. 8 No. 2 (2025): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v8i2.149

Soybean production in Indonesia currently does not meet national demand. However, there is potential to increase production by utilizing sub-optimal lands, such as Regosol soils. Regosol soils are characterized by a high sand content, low fertility, and poor water-holding capacity, which exposes plants to drought stress. To enhance soybean productivity under such conditions, technological innovations are needed, including the use of biofertilizers and nanotechnology. The Anjasmoro soybean variety is known to be relatively sensitive to drought stress. The application of Plant Growth-Promoting Rhizobacteria (PGPR) and nanosilica is expected to improve nutrient uptake, stimulate the production of growth-promoting hormones, and enhance plant tolerance to drought stress. This study aimed to evaluate the growth and yield of the Anjasmoro soybean variety in response to PGPR and nanosilica fertilization. A factorial Completely Randomized Design (CRD) was employed, consisting of nanosilica concentrations (0, 100, and 200 ppm) and PGPR concentrations (0, 5, 10, and 15%). The results indicated that PGPR and nanosilica fertilization improved soybean tolerance to drought stress. The most effective treatment for enhancing the growth and yield of the Anjasmoro soybean variety on Regosol soil was the application of 10% PGPR combined with 200 ppm nanosilica. This treatment significantly increased pod number, pod weight, 100-seed weight, fresh shoot weight, dry shoot weight, fresh root weight, and chlorophyll content. Additionally, the application of 5% PGPR combined with 200 ppm nanosilica resulted in the best seed quality

In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Risma Wiharyanti; Ixora Sartika Mercuriani; Evy Yulianti; Paramita Cahyaningrum Kuswandi
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA

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