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Hikmawan Wahyu Sulistomo
Departemen Farmakologi Fakultas Kedokteran Universitas Brawijaya
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Pengaruh Ekstrak Etanol Daun Inggu (Ruta angustifolia) terhadap Tebal Otot Polos Tuba Fallopi Tikus Strain Wistar Betina Novida Ariani; Hikmawan Wahyu Sulistomo; Yolanda Eldamayanti
Journal of Issues in Midwifery Vol. 6 No. 3 (2022)
Publisher : Journal of Issues in Midwifery

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.JOIM.2022.006.03.3

Abstract

ABSTRAK: Latar Belakang : Tingginya penghentian pemakaian kontrasepsi hormonal disebabkan oleh efek samping yang dialami akseptor KB menjadi latar belakang penelitian penggunaan tanaman herbal sebagai antifertilitas pengganti KB hormonal yang memiliki efek minimal. Daun tanaman inggu (Ruta angsutifolia) memiliki kandungan senyawa yang diduga mengganggu kerja hormonal sehingga dapat menjadi antifertilitas seperti saponin, tanin, chalepin dan chalepensin. Tujuan : Untuk menguji pengaruh ekstrak etanol daun inggu terhadap tebal otot polos tuba fallopi tikus putih. Metode : Penelitian experimental menggunakan randomized post-test only controlled group sebagi rancangannya dilakukan dari Februari hingga April 2022. Hewan coba (tikus putih Strain Wistar) dibagi menjadi 4 kelompok dengan perlakuan dosis (0; 0,4; 0,8; dan 1,2 g/kg BB). Pengukuran tebal otot polos tuba fallopi pada slide histopatologi memakai mikroskop jenis Olympus CX23 dan aplikasi ImageJ. Hasil : tebal otot polos organ tuba fallopi tikus menurun berbanding terbalik dengan dosis ekstrak etanol daun inggu yang meningkat dengan hasil signifikan secara statistik (p= 0,046). Kesimpulan : ketebalan otot polos organ tuba fallopi menunurun akibat ekstrak etanol daun inggu yang diberikan dengan dosis optimal 1,2 g/kg BB. Kata Kunci: daun inggu, tebal otot polos tuba fallopi, kontrasepsi
The Effect of Sub-Acute Inhalation Exposure to Polyethylene Micro-Nano Plastics on the Histopathological Features of the Mammary Glands in Female Wistar White Rats (Rattus Norvegicus) Ihda Dian Kusuma; Laksmitha Janasti; Riana Trinovita Sari; Britania Laila Nanda; Hikmawan Wahyu Sulistomo; Nurdiana Nurdiana
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1534

Abstract

Most household appliances are made of plastic derived from synthetic petroleum and result from polymerization processes. One type of plastic is Polyethylene (PE). Polyethylene (PE) contains antimony trioxide compounds that are carcinogenic if ingested in excessive amounts, triggering cancer and oxidative stress. This effect can be assessed by measuring malondialdehyde (MDA) levels in the body. This study aims to determine the proliferation of abnormal cells in the lactiferous ducts and mammary gland acini, as well as the increase in MDA levels. The research method used is a true experimental design with a Randomized Post Test Only Group Design. This study used the mammary organs and blood of female white rats exposed to PE for 28 days. The number of samples used in this study was 12 female white rats. The results showed significant differences in the mammary glands' histopathological features and MDA levels. Based on the Independent T-Test results on the number of cell layers in the lactiferous ducts, acini, and the number of acini (p<0.05) and the Mann-Whitney test on MDA levels (p<0.05). Significant changes in the histopathological features and MDA levels were observed in the mammary glands exposed to polyethylene (PE) plastic. Exposure to micro-nano plastics of PE type at a dose of 15 mg/m3 for 28 days differed significantly in the MDA levels of mammary glands, histopathological picture of mammary glands at cell proliferation events, both in the lactiferous ducts and acini cells. In addition, exposure to PE plastic has the potential to cause health problems in the breast organs if exposed for a long time and in excess doses.
Evaluation of Hematotoxicity in Female Wistar Rats Following Sub-Acute Inhalation Exposure to Polyethylene Microplastic Hikmawan Wahyu Sulistomo; Anisa Setyowati; Melani Chysti Situmorang; Ita Sulistiani; Dewi Azar Nuria Wardani; Kharisma Ciptaning Gusti; Nurdiana Nurdiana; Ihda Dian Kusuma; Bambang Rahardjo; Subandi Reksohusodo
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1585

Abstract

Polyethylene (PE) becomes a source of microplastics that can be widely distributed through the digestive and respiratory systems. However, its effects on blood cells are still being investigated. This study aims to analyze the impact of Polyethylene Microplastic (PE-MPs) exposure on the blood of female rats, including erythrocytes, leukocytes, and platelets. This study used female Wistar rats, which were divided into control and PE-MP groups. PE-MP was administered via whole-body inhalation at a concentration of 15 mg/m³ for 4 hours daily for 28 days. The absorption of plastic particles detected in the human bloodstream is likely to occur through mucosal contact (either through ingestion or inhalation). After the exposure period, the rats were euthanized to collect blood samples through the heart. A complete blood count was performed using an automatic hematology analyzer, and blood morphology was analyzed using thin blood smears. This study used the Mann-Whitney test. PE-MP exposure increased erythrocyte and platelet counts without a corresponding rise in leukocytes. Erythrocytes showed abnormal morphology (12.73% with ovalocytes and tear-shaped cells). Erythrocyte indices (MCV, MCH, MCHC) showed no significant differences. Platelet count rose by 1.7% (p-value= 0.017). Leukocyte and neutrophil counts were lower (0.84 and 0.94 times lower, respectively), while lymphocytes and monocytes were higher (1.03 and 1.61 times higher, respectively) in the PE-MP group compared to controls. The neutrophil-to-lymphocyte ratio did not differ significantly. PE-MP exposure in rats disrupts blood parameters, altering erythrocyte morphology and increasing platelet counts. Potential causes include oxidative stress, immune responses, and compensatory mechanisms. Study limitations include a small sample size and exclusive focus on inhalation exposure. Integrating multiple exposure routes (inhalation, ingestion, dermal) could offer a broader view of microplastic impacts. Future research with larger samples, diverse doses and durations, and exploration of additional markers or organ-specific effects is crucial for understanding PE-MP toxicity in real-world scenarios.               
Co-Authors Anisa Setyowati Bambang Rahardjo Britania Laila Nanda Dewi Azar Nuria Wardani Ihda Dian Kusuma Ita Sulistiani Kharisma Ciptaning Gusti Laksmitha Janasti Melani Chysti Situmorang Novida Ariani Nurdiana Nurdiana Nurdiana Nurdiana Riana Trinovita Sari Subandi Subandi Yolanda Eldamayanti