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Fitmawati
Universitas Riau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Antibacterial Activity of Extracts of Wild Mango (Mangifera spp.), Ruellia tuberosa L and Leucobryum sp. Causes of Gangrene in Patients with Diabetes Mellitus Rodesia Mustika Roza; Fitmawati; Hari Kapli; Fitra Suzanti
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4339

Abstract

Plants are the main medicinal source that exists around the world, due to the content of bioactive compounds such as flavonoids, alkaloids, saponins, polyphenols, tannins, terpenoids, and essential oils. These bioactive compounds have antimicrobial activity. The purpose of this study was to analyze the antibacterial activity of a combination extracts of Sumatran wild mango (M. sumatrana, and M. foetida var. Batu), R. tuberosa L.  and Leucobryum sp. against bacteria that cause gangrene wounds in diabetics. Preparation of plant extraction is carried out by the method of maceration using 70% alcohol. Testing of antibacterial activity using the disc paper method 6 mm in diameter at an extract concentration of 10% with seven treatments (P1-P7). The test results obtained the largest inhibitory zone diameter against Staphyococcus aureus ATCC 6538 and S. epidermidis ATCC 12228 both shown in combination II extracts at P7 treatment (2:3:1 ratio), 9.04±1.42 mm and 8.85±0.21 mm, respectively. The results of the inhibitory zone resulting from the combination extract test included criteria of resistance to Staphylococcus. In the positive control, the diameter of the chloramphenicol inhibition zone against S. aureus belongs to the sensitive category, but to S. epidermidis it belongs to the resistant category. Combination of methanol extract from wild mango, R. tuberosa L and Leucobryum sp. has the opportunity as the candidate for natural-based antibiotics to replace synthetic antibiotics.
Antibacterial Activity of Bitter Drugs from Lingga Malay Ethnic in Riau Archipelago Against Bacterial Causing Skin and Diarrhea Diseases Rahma Dhani; Rodesia Mustika Roza; Fitmawati
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4430

Abstract

Infection is the major threat to humanity caused by infectious agents. The most common infectious agent of human is the cause of skin and diarrhea diseases. Melayu Lingga people uses alternative medicine called obat pahit potion to treat skin and diarrhea diseases and mixes by Praktisi Obat Tradisional (POT). The purpose of this research was to determine antibacterial activity of the methanol extract from obat pahit potion against bacteria that cause skin diseases (Staphylococcus aureus ATCC 12600, Staphylococcus epidermidis ATCC 12228, Pseudomonas aeruginosa ATCC 10145) and diarrhea (Escherichia coli ATCC 11775, Salmonella typhi ATCC 14028, Shigella flexneri ATCC 12022) by using disc diffusion method with variation of extract concentration (0.75 gr/ml, 0.5 gr/ml, and 0.25 gr/ml). Antibacterial activity was determined by measuring the inhibitory zone formed around the disc. Based on the result of antibacterial activity test, the largest inhibitory zone was found in S.aureus ATCC 12600, S.epidermidis ATCC 12228 and P.aeruginosa ATCC 10145 shown in POT Musai at 0.25 mg/ml concentrations with value 6,61 mm; 6,10 mm; and 10,11 mm. Meanwhile, antibacterial activity against E.coli ATCC 11775 shown in TMP Musai at 0.5 gr/ml concentration with value 4,47 mm. Antibacterial activity against S.typhi ATCC 14028 and S.flexneri ATCC 12022 shown in TMP Musai at 0.25 gr/ml concentration with value 8,20 mm and 11,73 mm.
Co-Authors Fitra Suzanti Hari Kapli Rahma Dhani Rodesia Mustika Roza