Garuda - Garba Rujukan Digital

POTENSI JAMUR INDIGENUS RIAU (Penicillium sp.PN6) DAN Neptunia oleracea UNTUK BIOREMEDIASI OIL SLUDGE Wahyu Lestari; Atria Martina; Rodesia Mustika Roza; Imelda Wardani
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Abstrak Lumpur minyak bumi (oil sludge) memiliki kandungan kontaminan petroleum hidrokarbon terbesar, karenanya pengolahan limbah oil sludge perlu dilakukan agar tidak mencemari lingkungan. Penelitian yang menguji kemampuan jamur indigenus Riau (Penicillium sp. PN6) dan legum akuatik (Neptunia oleracea) dalam meremediasi senyawa hidrokarbon dengan pengukuran total petroleum hidrokarbon (TPH) pada limbah oil sludge telah dilakukan. Pertumbuhan N. oleracea dilakukan pada media limbah oil sludge dengan konsentrasi 25, 50, 75, dan 100%. Selanjutnya dilakukan pemberian inokulum Penicillium sp. PN6 ke dalam media pertumbuhan. Hasil penelitian menunjukkan bahwa persentase pertumbuhan N. oleracea adalah 95,58; 85,87; 25,55; dan 0%, berturut-turut pada limbah oil sludge 25, 50, 75, dan 100%. Terjadi penurunan konsentrasi TPH awal pada semua perlakuan di akhir pengamatan. Penicillium sp. PN6 meningkatkan degradasi TPH dari limbah oil sludge dibandingkan dengan kombinasi Penicillium sp. PN6 dengan N. oleracea, tetapi kombinasi Penicillium sp.PN6 dengan N. oleracea meningkatkan persentase penurunan TPH dari limbah oil sludge dibandingkan dengan menggunakan Penicillium sp. PN6 saja. Penicillium sp. PN6 dan kombinasinya dengan N. oleracea sama-sama mampu menurunkan pH limbah oil sludge.Abstract Oil sludge contains the largest contaminant of petroleum hydrocarbon that needs treatment to prevent its pollution into the environment. A study examining the ability of a Riau indigenous fungi (Penicillium sp. PN6) and an aquatic legume (Neptunia oleracea) to remediate hydrocarbon compounds by determining total petroleum hydrocarbon (TPH) in oil sludge waste has been conducted. The growth of N. oleracea was performed in the oil sludge waste media by concentrations of 25, 50, 75, and 100%. The medium was inoculated with Penicillium sp. PN6. The results showed that the percentage of growth of N. oleracea were 95.58; 85.87; 25.55; and 0%, in the oil sludge waste of 25, 50, 75, and 100%, respectively. There was a decrease in initial TPH concentration at all treatments at the end of the observation. Penicillium sp. PN6 increased more TPH degradation of the oil sludge waste compared to a combination of Penicillium sp. PN6 and N.oleracea, but the combination increased the decrease percentage of TPH compared to those that used Penicillium sp. PN6 only. Penicillium sp. PN6 and its combination with N. oleracea are both able to decrease pH of the oil sludge waste. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 6350

AKTIVITAS ANTIFUNGI ISOLAT AKTINOMISETES ARBORETUM UNIVERSITAS RIAU TERHADAP JAMUR Fusarium oxysporum f.sp lycopersici DAN Ganoderma boninense Vista Queendy; Rodesia Mustika Roza
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakRendahnya produktivitas lahan pertanian di Indonesia berkaitan dengan serangan penyakit tanaman, contohnya penyakit layu Fusarium pada tanaman tomat dan busuk pangkal batang pada tanaman kelapa sawit yang disebabkan oleh Ganoderma boninense. Penelitian sebelumnya telah berhasil mengisolasi aktinomisetes dari tanah Arboretum Universitas Riau, namun belum diketahui kemampuannya dalam menghambat jamur Fusarium oxysporum f.sp lycopersici dan Ganoderma boninense. Penelitian ini bertujuan untuk menguji 32 isolat aktinomisetes terhadap jamur target dengan metode difusi agar. Aktinomisetes potensial yang terpilih, dilanjutkan untuk produksi senyawa antifungi dengan lama waktu fermentasi yang berbeda (4, 5, dan 6 hari) dan diuji dengan metode difusi kertas cakram. Hasil penelitian uji aktivitas antifungi dengan metode difusi agar menunjukkan 31 isolat aktinomisetes mampu menghambat pertumbuhan jamur F. oxysporum f.sp lycopersici dan isolat A2.01 memiliki zona hambat tertinggi yaitu sebesar 36,10 mm. Sebanyak 28 isolat aktinomisetes mampu menghambat jamur G. boninense dan isolat D2.28 memiliki zona hambat tertinggi, yaitu sebesar 45,71 mm. Isolat aktinomisetes Arboretum Universitas Riau sebagian besar dapat menghambat pertumbuhan kedua jamur target dengan metode difusi agar, namun hasil uji senyawa antifungi isolat potensial dengan metode difusi kertas cakram belum mampu membentuk zona hambat. Isolat aktinomisetes potensial dapat diaplikasikan menggunakan kultur langsung secara in vivo.Abstract The low productivity of agricultural land in Indonesia occurred because of plant diseases attack such as Wilt Fusarium in tomato plant and Basal Stem Rot disease in palm oil caused by Ganoderma boninese. Previous research had successfully isolated the actinomycetes from soil of Arboretum University of Riau However, the antifungal potency of those isolates against Fusarium oxysporum f. sp lycopersici and Ganoderma boninense had not been known. This research aimed to screening 32 actinomycetes isolates against the fungal target by using agar diffusion method. The selected actinomycetes were continued to produce crude antifungal by different duration of fermentation (4, 5 and 6 days) using disc diffusion method. The result of the antifungal activity using agar diffusion method showed that there were 31 isolates that able to inhibit the F. oxysporum f. sp lycopersici and isolate A2.01 showed the highest inhibition zone by 36.10 mm. There were 28 isolates that able to inhibit the G. boninense and isolate D2.28 showed the highest inhibition zone by 45.71 mm. Almost all of the isolate was able to inhibit both of two fungals targets with diffussion agar method, but the crude antifungal compound of the potential isolate by disc diffusion method could not inhibit both of the fungal targets. The potential actinomycetes isolate was suggested to be applied as a culture directly in vivo.

ANTAGONISTIC TEST OF RIAU LOCAL FUNGAL ISOLATES AGAINST SOME PATHOGENIC IN CULTIVATED PLANTS Nia Safitri; Atria Martina; Rodesia Mustika Roza
Al-Kauniyah: Jurnal Biologi Vol 12, No 2 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v12i2.8730

Tanaman budi daya merupakan tanaman yang sering diserang oleh cendawan pathogen, sehingga mengakibatkan penurunan populasi dan produksi tanaman. Pengendalian hayati dengan cendawan antagonis merupakan salah satu metode yang paling efektif dan lebih ramah lingkungan dalam menekan pertumbuhan patogen tanaman. Penelitian ini bertujuan untuk menguji aktivitas antagonis cendawan isolat lokal Riau terhadap beberapa cendawan patogen pada tanaman budi daya. Uji antagonis dilakukan secara in vitro dengan metode dual culture menggunakan lima belas cendawan isolat lokal Riau terhadap Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus dan Colletotrichum sansevieria. Hasil penelitian menunjukkan bahwa Trichoderma sp. PNE 4 memiliki aktivitas antagonis tertinggi dan isolat FER C1 serta isolat LLB07 hanya memiliki aktivitas antagonis yang tinggi dalam menekan pertumbuhan cendawan patogen. Trichoderma sp. PNE 4 mampu menghambat pertumbuhan miselium F. oxysporum sebesar 85,30%, G. Philippii (100%), G. boninense (100%), dan C. sansevieria (100%). Isolat FER C1 hanya menghambat R. Microporus (50,39%) dan isolat LLB07 menghambat G. philippii (52,20%). Trichoderma sp. PNE 4 merupakan cendawan uji yang terpilih sebagai cendawan antagonis, karena memiliki kemampuan daya hambat >70%.Abstract Cultivated plants are often attacked by pathogenic fungi resulting in a decline of population and crop production. Biocontrol with antagonistic fungi is one of the most effective and environmentally friendly methods in suppressing the growth of plant pathogens. This study aims to examine the antagonistic activity of local isolates fungi Riau against some pathogenic fungi on cultivated plants. The antagonistic test was performed in vitro by dual culture method using fifteen local isolates fungal Riau against Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus and Colletotrichum sansevieria. The results showed that Trichoderma sp. PNE 4 isolate exhibited highest activites and FER C1 and LLB07 isolates exhibited high activities suppressed the growth of the fungal pathogen. Trichoderma sp. PNE 4 isolate inhibited mycelial growth F. oxysporum (85.30%), G. philippii (100%), G. boninense (100%) and C. sansevieria (100%). FER C1 isolate only inhibited R. microporus (50.39%), and LLB07 isolate inhibited G. philippii (52.20%). Trichoderma sp. PNE 4 isolate is test isolates as fungal antagonistic.

Penerapan teknologi hidroponik sistem deep flow technique sebagai usaha peningkatan pendapatan petani di Desa Sungai Bawang Fitmawati Fitmawati; Isnaini Isnaini; Siti Fatonah; Nery Sofiyanti; Rodesia Mustika Roza
Riau Journal of Empowerment Vol 1 No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The target becomes a self-reliant village must be accompanied by strengthening village ability in supplying the basic needs independently. Vegetables are one of the crucial basic needs to achieve a healthy and prosperous society. The demand for vegetables in Sungai Bawang Village, Kuantan Singingi Regency, is constrained by the available land for vegetable farming. Hydroponic planting system offers the technique without soil that can be implemented anywhere. It has added value in providing clean and hygienic vegetables. Hydroponics technology system of Deep Flow Technique able to provide water and oxygen for plants and suitable for leafy vegetables. Hydroponic is appropriate to be implemented in Sungai Bawang village and did not require huge land in applying this technology. The mentoring strategy is carried out by counseling and demonstration of direct planting stages and the practice by the community. This activity gave a positive influence on the community as well as many people who want to apply this technology. This was the first step in increasing the income of the community, especially farmers. And for the community in general, this technology facilitates the people to get vegetables easily at an affordable price.

Kebun Organik Di Masa Adaptasi Kebiasaan Baru Pandemi Covid-19 Dengan Memanfaatkan Sampah Rumah Tangga Rodesia Mustika Roza; Atria Martina; Hari Kapli; Bernadeta Leni Fibriarti; Wahyu Lestari
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.658-664

The World Health Organization has designated Covid-19 as a pandemic that causes activities outside the home to be restricted. In order to maintain health during the pandemic, many people are starting to choose foodstuffs produced from organic farming. The purpose of this activity is to provide information and motivation to society for reuse household waste with appropriate technology to produce compost. This service activity was carried out in Air Dingin Village, Bukit Raya District, Pekanbaru. The initial stage conveys material about the importance of maintaining health during the pandemic by consuming organic vegetables and fruits grown in their respective yards. Furthermore, the practice of making compost using the direct-use compost method and composting in a composter pot. Composting that reuses household waste has succeeded in opening up insight into the importance of the community's role in personal and environmental health and the public's understanding that a healthy life doesn't have to be expensive. In this activity, the participants showed their enthusiasm and stated that they would practice and share their knowledge with others in making compost to overcome the problem of household waste. This activity is also beneficial to keep the environment clean, healthy and beautiful.

Antibacterial Activity of Extracts of Wild Mango (Mangifera spp.), Ruellia tuberosa L and Leucobryum sp. Causes of Gangrene in Patients with Diabetes Mellitus Rodesia Mustika Roza; Fitmawati; Hari Kapli; Fitra Suzanti
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4339

Plants are the main medicinal source that exists around the world, due to the content of bioactive compounds such as flavonoids, alkaloids, saponins, polyphenols, tannins, terpenoids, and essential oils. These bioactive compounds have antimicrobial activity. The purpose of this study was to analyze the antibacterial activity of a combination extracts of Sumatran wild mango (M. sumatrana, and M. foetida var. Batu), R. tuberosa L. and Leucobryum sp. against bacteria that cause gangrene wounds in diabetics. Preparation of plant extraction is carried out by the method of maceration using 70% alcohol. Testing of antibacterial activity using the disc paper method 6 mm in diameter at an extract concentration of 10% with seven treatments (P1-P7). The test results obtained the largest inhibitory zone diameter against Staphyococcus aureus ATCC 6538 and S. epidermidis ATCC 12228 both shown in combination II extracts at P7 treatment (2:3:1 ratio), 9.04±1.42 mm and 8.85±0.21 mm, respectively. The results of the inhibitory zone resulting from the combination extract test included criteria of resistance to Staphylococcus. In the positive control, the diameter of the chloramphenicol inhibition zone against S. aureus belongs to the sensitive category, but to S. epidermidis it belongs to the resistant category. Combination of methanol extract from wild mango, R. tuberosa L and Leucobryum sp. has the opportunity as the candidate for natural-based antibiotics to replace synthetic antibiotics.

Antibacterial Activity of Bitter Drugs from Lingga Malay Ethnic in Riau Archipelago Against Bacterial Causing Skin and Diarrhea Diseases Rahma Dhani; Rodesia Mustika Roza; Fitmawati
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4430

Infection is the major threat to humanity caused by infectious agents. The most common infectious agent of human is the cause of skin and diarrhea diseases. Melayu Lingga people uses alternative medicine called obat pahit potion to treat skin and diarrhea diseases and mixes by Praktisi Obat Tradisional (POT). The purpose of this research was to determine antibacterial activity of the methanol extract from obat pahit potion against bacteria that cause skin diseases (Staphylococcus aureus ATCC 12600, Staphylococcus epidermidis ATCC 12228, Pseudomonas aeruginosa ATCC 10145) and diarrhea (Escherichia coli ATCC 11775, Salmonella typhi ATCC 14028, Shigella flexneri ATCC 12022) by using disc diffusion method with variation of extract concentration (0.75 gr/ml, 0.5 gr/ml, and 0.25 gr/ml). Antibacterial activity was determined by measuring the inhibitory zone formed around the disc. Based on the result of antibacterial activity test, the largest inhibitory zone was found in S.aureus ATCC 12600, S.epidermidis ATCC 12228 and P.aeruginosa ATCC 10145 shown in POT Musai at 0.25 mg/ml concentrations with value 6,61 mm; 6,10 mm; and 10,11 mm. Meanwhile, antibacterial activity against E.coli ATCC 11775 shown in TMP Musai at 0.5 gr/ml concentration with value 4,47 mm. Antibacterial activity against S.typhi ATCC 14028 and S.flexneri ATCC 12022 shown in TMP Musai at 0.25 gr/ml concentration with value 8,20 mm and 11,73 mm.

The Potential of Wild Mango Leaves from Sumatera as the Immunostimulant Agent Fitmawati Fitmawati; Erwina Juliantari; Agus Saputra; Patar Sinaga; Rodesia Mustika Roza; Mayta Novaliza Isda
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.16549

Wild Mango from Sumatera is potentially new immunostimulant. It contains mangiferin, which is potentially become the immunostimulant candidate. This study aimed to prove the immunostimulatory effect of wild mango leaves extract on white rat peritoneum fluid induced by Staphylococcus aureus bacteria. The immunostimulatory effect was determined based on the activity and capacity of macrophage phagocytosis. This study used 54 male white rats about 130 g - 290 g. Based on the results,the highest phagocytosis activity of macrophage cells was found in ethanol extract of Mangga Hutan leaves dose 2 with a percentage of phagocytosis activity was 84%. The highest phagocytosis capacity of the macrophage cells was also found in ethanol extract of Mangga Hutan leaves dose 2 with an average phagocytic capacity of 171.67 from 50 active macrophages. Results of this study indicated that wild mango leaves from Sumatera has the potential immunostimulant activity. This indicates that the wild mango have the potential for therapeutic efficacy for the prevention of degenerative diseases caused by immune deficiencies.

Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago Fitmawati Fitmawati; Nery Sofiyanti; Rodesia Mustika Roza; Isnaini Isnaini; Yulisa Resti Irawan; Dhaniel Ridho Winata; Awal Prichatin Kusumo Dewi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9808

Obat Pahit is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely Bauhunia semibifida, Cnestis palala and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in Obat Pahit from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of Obat Pahit from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of Bauhinia semibifida (6.6247), Penawa Root (5.0124) and Cnestis palala (5.9968) were much lower than IC50 of mangosteen’s rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesia’s local ethnicities.

Immunomodulatory Effectiveness of Aqueous Obat Pahit Extract of Lingga Malay Ethnic on White Rats (Rattus novergicus) Fitmawati Fitmawati; Rodesia Mustika Roza; Nery Sofiyanti; Isnaini Isnaini; Febrian Lailatul Fitri; Desi Paramita; Awal Prichatin Kusumo Dewi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10496

Obat pahit has been generally known and believed by Lingga Malay society as anti-aging agent. However, the study of Obat pahit is not scientifically proven. This research was aimed to prove immunomodulatory ability of Obat pahit potion from Lingga, Riau Archipelago. This study used white rats as an animal modelling, and Staphylococcus aureus as bacteria tester. The rats had been treated with aqueous Obat pahit extract from three TMPs on dose scales of 0.09, 0.18 and 0.27 mL/200g of body weight through oral administration for 7 days. Furthermore, on the 8th days, the experiment animals were injected by the preparation of bacteria tester through intraperitoneal administration in the amount of 0.5 mL/200 gram of body weigth and subsequently incubated for 1 hour after the injection. There were 2 observed parameters on this study, i.e efectivity and capacity of phagocytosis by leukocytes. The observation of leukocytes-phagocytocis activity was carried out by making a smear preparat samples of peritoneum fluid from rats. After the observation under microscope on a magnification of 100 times. The result was obtained the Obat pahit from Kalan PMT swere more effective on dose 2, while from SP4 and Linau TMPs were much more effective on dose 1. It is therefore, using these data of the results, the advanced doses scale of this Obat pahit would not be necessary. Obat pahit potion from Malay Lingga Malay Ethnic could become raw materials of immunomodulatory herbal medicine based on traditional knowledge. It also potentially as a standardized herbal.

Title

Found 10 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 10 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 10 Documents
Search